Mercurial > repos > q2d2 > qiime2__deblur__denoise_16s
changeset 1:2572fc0f88f2 draft
planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__deblur commit 69da7976573cc07a363ac66bdacc9269d7cd3732
author | q2d2 |
---|---|
date | Fri, 13 Jan 2023 22:42:34 +0000 |
parents | 0cba463cfb72 |
children | b883cbea5623 |
files | qiime2__deblur__denoise_16S.xml test-data/.gitkeep test-data/denoise_16S.test0.demux-filtered.qza test-data/visualize_stats.test0.deblur-stats.qza |
diffstat | 3 files changed, 65 insertions(+), 8 deletions(-) [+] |
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--- a/qiime2__deblur__denoise_16S.xml Mon Aug 29 19:25:20 2022 +0000 +++ b/qiime2__deblur__denoise_16S.xml Fri Jan 13 22:42:34 2023 +0000 @@ -1,31 +1,31 @@ <?xml version='1.0' encoding='utf-8'?> <!-- -Copyright (c) 2022, QIIME 2 development team. +Copyright (c) 2023, QIIME 2 development team. Distributed under the terms of the Modified BSD License. (SPDX: BSD-3-Clause) --> <!-- This tool was automatically generated by: - q2galaxy (version: 2022.8.1) + q2galaxy (version: 2022.11.1) for: - qiime2 (version: 2022.8.1) + qiime2 (version: 2022.11.1) --> -<tool name="qiime2 deblur denoise-16S" id="qiime2__deblur__denoise_16S" version="2022.8.0+q2galaxy.2022.8.1.2" profile="22.05" license="BSD-3-Clause"> +<tool name="qiime2 deblur denoise-16S" id="qiime2__deblur__denoise_16S" version="2022.11.1+q2galaxy.2022.11.1.2" profile="22.05" license="BSD-3-Clause"> <description>Deblur sequences using a 16S positive filter.</description> <requirements> - <container type="docker">quay.io/qiime2/core:2022.8</container> + <container type="docker">quay.io/qiime2/core:2022.11</container> </requirements> <version_command>q2galaxy version deblur</version_command> - <command detect_errors="aggressive">q2galaxy run deblur denoise_16S '$inputs'</command> + <command detect_errors="exit_code">q2galaxy run deblur denoise_16S '$inputs'</command> <configfiles> <inputs name="inputs" data_style="paths"/> </configfiles> <inputs> <param name="demultiplexed_seqs" type="data" format="qza" label="demultiplexed_seqs: SampleData[SequencesWithQuality | PairedEndSequencesWithQuality | JoinedSequencesWithQuality]" help="[required] The demultiplexed sequences to be denoised."> <options options_filter_attribute="metadata.semantic_type"> + <filter type="add_value" value="SampleData[SequencesWithQuality]"/> <filter type="add_value" value="SampleData[PairedEndSequencesWithQuality]"/> <filter type="add_value" value="SampleData[JoinedSequencesWithQuality]"/> - <filter type="add_value" value="SampleData[SequencesWithQuality]"/> </options> <validator type="expression" message="Incompatible type">hasattr(value.metadata, "semantic_type") and value.metadata.semantic_type in ['SampleData[JoinedSequencesWithQuality]', 'SampleData[PairedEndSequencesWithQuality]', 'SampleData[SequencesWithQuality]']</validator> </param> @@ -47,7 +47,34 @@ <data name="representative_sequences" format="qza" label="${tool.name} on ${on_string}: representative_sequences.qza" from_work_dir="representative_sequences.qza"/> <data name="stats" format="qza" label="${tool.name} on ${on_string}: stats.qza" from_work_dir="stats.qza"/> </outputs> - <tests/> + <tests> + <test> + <param name="demultiplexed_seqs" value="denoise_16S.test0.demux-filtered.qza" ftype="qza"/> + <param name="trim_length" value="120"/> + <param name="sample_stats" value="__q2galaxy__::literal::True"/> + <output name="representative_sequences" ftype="qza"> + <assert_contents> + <has_archive_member path="[0-9a-f]{8}-[0-9a-f]{4}-[4][0-9a-f]{3}-[89ab][0-9a-f]{3}-[0-9a-f]{12}\/metadata.yaml"> + <has_line_matching expression="type: FeatureData\[Sequence\]"/> + </has_archive_member> + </assert_contents> + </output> + <output name="table" ftype="qza"> + <assert_contents> + <has_archive_member path="[0-9a-f]{8}-[0-9a-f]{4}-[4][0-9a-f]{3}-[89ab][0-9a-f]{3}-[0-9a-f]{12}\/metadata.yaml"> + <has_line_matching expression="type: FeatureTable\[Frequency\]"/> + </has_archive_member> + </assert_contents> + </output> + <output name="stats" ftype="qza"> + <assert_contents> + <has_archive_member path="[0-9a-f]{8}-[0-9a-f]{4}-[4][0-9a-f]{3}-[89ab][0-9a-f]{3}-[0-9a-f]{12}\/metadata.yaml"> + <has_line_matching expression="type: DeblurStats"/> + </has_archive_member> + </assert_contents> + </output> + </test> + </tests> <help> QIIME 2: deblur denoise-16S =========================== @@ -66,6 +93,36 @@ ------------ Perform sequence quality control for Illumina data using the Deblur workflow with a 16S reference as a positive filter. Only forward reads are supported at this time. The specific reference used is the 88% OTUs from Greengenes 13_8. This mode of operation should only be used when data were generated from a 16S amplicon protocol on an Illumina platform. The reference is only used to assess whether each sequence is likely to be 16S by a local alignment using SortMeRNA with a permissive e-value; the reference is not used to characterize the sequences. +Examples: +--------- + +denoise_16S +*********** +Using the ``qiime2 deblur denoise-16S`` tool: + #. Set *"demultiplexed_seqs"* to ``#: demux-filtered.qza`` + #. Set *"trim_length"* to ``120`` + #. Expand the ``additional options`` section + + - Set *"sample_stats"* to ``Yes`` + + #. Press the ``Execute`` button. + +Once completed, for each new entry in your history, use the ``Edit`` button to set the name as follows: + (Renaming is optional, but it will make any subsequent steps easier to complete.) + + .. list-table:: + :align: left + :header-rows: 1 + + * - History Name + - *"Name"* to set (be sure to press ``Save``) + * - ``#: qiime2 deblur denoise-16S [...] : table.qza`` + - ``table.qza`` + * - ``#: qiime2 deblur denoise-16S [...] : representative_sequences.qza`` + - ``representative-sequences.qza`` + * - ``#: qiime2 deblur denoise-16S [...] : stats.qza`` + - ``denoising-stats.qza`` + |