Mercurial > repos > q2d2 > qiime2__demux__emp_single

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author q2d2
date Mon, 29 Aug 2022 19:27:37 +0000
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320c9af45b63 planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__demux commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1
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1 <?xml version='1.0' encoding='utf-8'?>
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2 <!--
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3 Copyright (c) 2022, QIIME 2 development team.
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4
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5 Distributed under the terms of the Modified BSD License. (SPDX: BSD-3-Clause)
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6 -->
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7 <!--
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8 This tool was automatically generated by:
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9 q2galaxy (version: 2022.8.1)
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10 for:
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11 qiime2 (version: 2022.8.1)
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12 -->
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13 <tool name="qiime2 demux emp-single" id="qiime2__demux__emp_single" version="2022.8.0+q2galaxy.2022.8.1.2" profile="22.05" license="BSD-3-Clause">
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14 <description>Demultiplex sequence data generated with the EMP protocol.</description>
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15 <requirements>
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16 <container type="docker">quay.io/qiime2/core:2022.8</container>
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17 </requirements>
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18 <version_command>q2galaxy version demux</version_command>
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19 <command detect_errors="aggressive">q2galaxy run demux emp_single '$inputs'</command>
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20 <configfiles>
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21 <inputs name="inputs" data_style="paths"/>
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22 </configfiles>
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23 <inputs>
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24 <param name="seqs" type="data" format="qza" label="seqs: RawSequences | EMPSingleEndSequences | EMPPairedEndSequences" help="[required] The single-end sequences to be demultiplexed.">
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25 <options options_filter_attribute="metadata.semantic_type">
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26 <filter type="add_value" value="RawSequences"/>
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27 <filter type="add_value" value="EMPSingleEndSequences"/>
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28 <filter type="add_value" value="EMPPairedEndSequences"/>
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29 </options>
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30 <validator type="expression" message="Incompatible type">hasattr(value.metadata, "semantic_type") and value.metadata.semantic_type in ['EMPPairedEndSequences', 'EMPSingleEndSequences', 'RawSequences']</validator>
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31 </param>
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32 <conditional name="barcodes">
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33 <param name="type" type="select" label="barcodes: MetadataColumn[Categorical]" help="[required] The sample metadata column containing the per-sample barcodes.">
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34 <option value="tsv" selected="true">Metadata from TSV</option>
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35 <option value="qza">Metadata from Artifact</option>
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36 </param>
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37 <when value="tsv">
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38 <param name="source" type="data" format="tabular,qiime2.tabular" label="Metadata Source"/>
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39 <param name="column" type="data_column" label="Column Name" data_ref="source" use_header_names="true">
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40 <validator type="expression" message="The first column cannot be selected (they are IDs).">value != "1"</validator>
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41 </param>
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42 </when>
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43 <when value="qza">
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44 <param name="source" type="data" format="qza" label="Metadata Source"/>
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45 <param name="column" type="text" label="Column Name">
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46 <validator type="empty_field"/>
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47 </param>
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48 </when>
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49 </conditional>
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50 <section name="__q2galaxy__GUI__section__extra_opts__" title="Click here for additional options">
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51 <param name="golay_error_correction" type="boolean" truevalue="__q2galaxy__::literal::True" falsevalue="__q2galaxy__::literal::False" checked="true" label="golay_error_correction: Bool" help="[default: Yes] Perform 12nt Golay error correction on the barcode reads."/>
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52 <param name="rev_comp_barcodes" type="boolean" truevalue="__q2galaxy__::literal::True" falsevalue="__q2galaxy__::literal::False" label="rev_comp_barcodes: Bool" help="[default: No] If provided, the barcode sequence reads will be reverse complemented prior to demultiplexing."/>
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53 <param name="rev_comp_mapping_barcodes" type="boolean" truevalue="__q2galaxy__::literal::True" falsevalue="__q2galaxy__::literal::False" label="rev_comp_mapping_barcodes: Bool" help="[default: No] If provided, the barcode sequences in the sample metadata will be reverse complemented prior to demultiplexing."/>
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54 <param name="ignore_description_mismatch" type="boolean" truevalue="__q2galaxy__::literal::True" falsevalue="__q2galaxy__::literal::False" label="ignore_description_mismatch: Bool" help="[default: No] If enabled, ignore mismatches in sequence record description fields."/>
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55 </section>
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56 </inputs>
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57 <outputs>
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58 <data name="per_sample_sequences" format="qza" label="${tool.name} on ${on_string}: per_sample_sequences.qza" from_work_dir="per_sample_sequences.qza"/>
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59 <data name="error_correction_details" format="qza" label="${tool.name} on ${on_string}: error_correction_details.qza" from_work_dir="error_correction_details.qza"/>
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60 </outputs>
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61 <tests/>
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62 <help>
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63 QIIME 2: demux emp-single
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64 =========================
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65 Demultiplex sequence data generated with the EMP protocol.
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66
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67
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68 Outputs:
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69 --------
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70 :per_sample_sequences.qza: The resulting demultiplexed sequences.
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71 :error_correction_details.qza: Detail about the barcode error corrections.
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72
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73 |
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74
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75 Description:
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76 ------------
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77 Demultiplex sequence data (i.e., map barcode reads to sample ids) for data generated with the Earth Microbiome Project (EMP) amplicon sequencing protocol. Details about this protocol can be found at http://www.earthmicrobiome.org/protocols-and-standards/
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78
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79
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80 |
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81
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82 </help>
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83 <citations>
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84 <citation type="doi">10.1038/nmeth.1184</citation>
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85 <citation type="doi">10.1101/gr.085464.108</citation>
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86 <citation type="doi">10.1038/s41587-019-0209-9</citation>
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87 </citations>
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88 </tool>