<?xml version='1.0' encoding='utf-8'?>
<!--
Copyright (c) 2024, QIIME 2 development team.

Distributed under the terms of the Modified BSD License. (SPDX: BSD-3-Clause)
-->
<!--
This tool was automatically generated by:
    q2galaxy (version: 2024.5.0)
for:
    qiime2 (version: 2024.5.0)
-->
<tool name="qiime2 demux subsample-paired" id="qiime2__demux__subsample_paired" version="2024.5.0+q2galaxy.2024.5.0" profile="22.05" license="BSD-3-Clause">
    <description>Subsample paired-end sequences without replacement.</description>
    <requirements>
        <container type="docker">quay.io/qiime2/amplicon:2024.5</container>
    </requirements>
    <version_command>q2galaxy version demux</version_command>
    <command detect_errors="exit_code">q2galaxy run demux subsample_paired '$inputs'</command>
    <configfiles>
        <inputs name="inputs" data_style="staging_path_and_source_path"/>
    </configfiles>
    <inputs>
        <param name="sequences" type="data" format="qza" label="sequences: SampleData[PairedEndSequencesWithQuality]" help="[required]  The demultiplexed sequences to be subsampled.">
            <options options_filter_attribute="metadata.semantic_type">
                <filter type="add_value" value="SampleData[PairedEndSequencesWithQuality]"/>
            </options>
            <validator type="expression" message="Incompatible type">hasattr(value.metadata, "semantic_type") and value.metadata.semantic_type in ['SampleData[PairedEndSequencesWithQuality]']</validator>
        </param>
        <param name="fraction" type="float" min="1e-06" max="0.999999" value="" label="fraction: Float % Range(0, 1, inclusive_start=False)" help="[required]  The fraction of sequences to retain in subsample."/>
    </inputs>
    <outputs>
        <data name="subsampled_sequences" format="qza" label="${tool.name} on ${on_string}: subsampled_sequences.qza" from_work_dir="subsampled_sequences.qza"/>
    </outputs>
    <tests/>
    <help>
QIIME 2: demux subsample-paired
===============================
Subsample paired-end sequences without replacement.


Outputs:
--------
:subsampled_sequences.qza: The subsampled sequences.

|  

Description:
------------
Generate a random subsample of paired-end sequences containing approximately the fraction of input sequences specified by the fraction parameter. The number of output samples will always be equal to the number of input samples, even if some of those samples contain no sequences after subsampling.


|  

</help>
    <citations>
        <citation type="doi">10.1038/s41587-019-0209-9</citation>
    </citations>
</tool>