changeset 0:af6d9ad14a0f draft

"planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_net commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
author qfabrepo
date Mon, 14 Sep 2020 08:13:43 +0000
parents
children 22abc415e142
files phyloseq_net.r phyloseq_net.xml test-data/GP.biom test-data/biom_out.html test-data/count.txt test-data/metadata.txt test-data/observation.txt test-data/test.html
diffstat 8 files changed, 7032 insertions(+), 0 deletions(-) [+]
line wrap: on
line diff
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/phyloseq_net.r	Mon Sep 14 08:13:43 2020 +0000
@@ -0,0 +1,147 @@
+library('getopt')
+library('data.table')
+suppressPackageStartupMessages(library('phyloseq'))
+suppressPackageStartupMessages(library('DESeq2'))
+
+Sys.setenv("DISPLAY"=":1")
+
+options(warn= -1)
+option_specification = matrix(c(
+   'infile','i',2,'character',
+   'metafile','m',2,'character',
+       'biom','b',2,'character',
+     'obsfile','o',2,'character',
+     'norm','n',2,'logical',
+     'xcolumn','x',2,'numeric',
+     'lcolumn','l',2,'numeric',
+     'outdir','d',2,'character',
+   'htmlfile','h',2,'character'
+),byrow=TRUE,ncol=4);
+
+
+options <- getopt(option_specification)
+options(bitmapType="cairo")
+
+matrix.format<-function(x) {
+    m<-as.matrix(x[,-1])
+    rownames(m)<-x[,1]
+    m
+}
+
+
+gm_mean = function(x, na.rm=TRUE){ exp(sum(log(x[x > 0]), na.rm=na.rm) / length(x))}
+
+
+tax_col_norm <- c("Kingdom","Phylum","Class","Order","Family","Genus","Species")
+tax_col_extra <- c("None","Kingdom","Phylum","Class","Order","Family","Genus","Species") 
+
+tax_col_norm_otu <- c("OTUID","Kingdom","Phylum","Class","Order","Family","Genus","Species")
+tax_col_extra_otu <- c("OTUID","None","Kingdom","Phylum","Class","Order","Family","Genus","Species")
+
+if (!is.null(options$outdir)) {
+  # Create the directory
+  dir.create(options$outdir,FALSE)
+}
+
+is.biom<-options$biom
+
+
+pdffile <- gsub("[ ]+", "", paste(options$outdir,"/pdffile.pdf"))
+pngfile_net <- gsub("[ ]+", "", paste(options$outdir,"/net.png"))
+htmlfile <- gsub("[ ]+", "", paste(options$htmlfile))
+
+if(is.biom=="set_biom"){
+
+galaxy_biom <- import_biom(options$infile)
+galaxy_map <- import_qiime_sample_data(options$metafile)
+
+number.of.tax.rank<-length(colnames(tax_table(galaxy_biom)))
+
+	if(number.of.tax.rank == 7){
+	    colnames(tax_table(galaxy_biom)) <- tax_col_norm
+	} else {
+	    colnames(tax_table(galaxy_biom)) <- tax_col_extra
+	}
+
+physeq_galaxy <- merge_phyloseq(galaxy_biom,galaxy_map)
+
+} else {
+
+count.table<-read.table(options$infile,header=T,sep="\t",comment.char="",stringsAsFactors = F)
+meta.table<-read.table(options$metafile,header=T,sep="\t",comment.char="",stringsAsFactors = F)
+tax.table<-read.table(options$obsfile,header=T,sep="\t",comment.char="",stringsAsFactors = F)
+
+
+colnames(count.table)<-gsub("^X","",colnames(count.table))
+colnames(meta.table)<-gsub("^X.","",colnames(meta.table))
+colnames(tax.table)<-gsub("^X.","",colnames(tax.table))
+
+count.table.formatted<-matrix.format(count.table)
+OTU<-otu_table(count.table.formatted,taxa_are_rows = TRUE)
+
+tax.table.new<-as.data.frame(cbind(tax.table[,1],t(as.data.table(strsplit(tax.table[,2],";")))))
+
+
+if(length(colnames(tax.table.new)) != length(tax_col_extra_otu)){
+colnames(tax.table.new)<-tax_col_norm_otu
+}else{
+colnames(tax.table.new)<-tax_col_extra_otu
+}
+
+tax.table.formatted<-matrix.format(tax.table.new)
+
+TAX<-tax_table(tax.table.formatted)
+
+physeq_galaxy <- phyloseq(OTU, TAX)
+
+
+galaxy_map<-meta.table
+
+rownames(galaxy_map)<-meta.table[,1]
+
+sampledata<-sample_data(as.data.frame(galaxy_map,row.names=sample_names(galaxy_map),stringsAsFactos=F))
+
+sample_data(physeq_galaxy)<-sampledata
+
+}
+
+
+x.selectedColumn<-colnames(galaxy_map)[options$xcolumn]
+l.selectedColumn<-colnames(galaxy_map)[options$lcolumn]
+
+### normalisation
+if(is.null(options$norm) || options$norm =="false"){
+suppressMessages(raw.count.deseq2.obj<-phyloseq_to_deseq2(physeq_galaxy,as.formula(paste('~',x.selectedColumn,sep=""))))
+geoMeans = apply(counts(raw.count.deseq2.obj), 1, gm_mean)
+
+deseq.obj = estimateSizeFactors(raw.count.deseq2.obj, geoMeans = geoMeans)
+deseq.obj.norm<-otu_table(as.matrix(counts(deseq.obj,normalized=T)),taxa_are_rows=TRUE)
+
+otu_table(physeq_galaxy)<-deseq.obj.norm
+}
+
+
+# Produce PDF file
+
+pdf(pdffile); 
+plot_net(physeq_galaxy,point_label=x.selectedColumn,color=l.selectedColumn)
+garbage<-dev.off();
+
+#Cairo(pngfile_net, type="png", bg="white",pointsize=12,dpi=100,units="in",width=6,height=6) 
+png(pngfile_net,units="in",width=6,height=6,pointsize=12,res=100,bg="white")
+plot_net(physeq_galaxy,point_label=x.selectedColumn,color=l.selectedColumn)
+garbage<-dev.off() 
+
+# Produce the HTML file
+htmlfile_handle <- file(htmlfile)
+html_output = c('<html><body>',
+	            '<table align="center">',
+		        '<tr>',
+		        '<td valign="middle" style="vertical-align:middle;">',
+                '<a href="pdffile.pdf"><img src="net.png"/></a>',
+		        '</td>',
+		        '</tr>',
+		        '</table>',
+                '</html></body>');
+writeLines(html_output, htmlfile_handle);
+close(htmlfile_handle);
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/phyloseq_net.xml	Mon Sep 14 08:13:43 2020 +0000
@@ -0,0 +1,125 @@
+<tool id="phyloseq_net" name="Phyloseq Network Plot" version="1.24.2" hidden="false">
+  <description>Phyloseq Network Plot</description>
+  <requirements>
+        <requirement type="package" version="3.4.1">r-base</requirement>
+        <requirement type="package" version="1.24.2">bioconductor-phyloseq</requirement>
+		<requirement type="package" version="1.20.0">bioconductor-deseq2</requirement>
+		<requirement type="package" version="1.10.4">r-data.table</requirement>
+        <requirement type="package" version="1.20.0">r-getopt</requirement>
+        <requirement type="package" version="9.18">ghostscript</requirement>
+  </requirements>
+  <version_command><![CDATA[
+          echo $(R --version | grep version | grep -v GNU)", phyloseq version" $(R --vanilla --slave -e "library(phyloseq); cat(sessionInfo()\$otherPkgs\$phyloseq\$Version)" 2> /dev/null | grep -v -i "WARNING: ")
+              ]]></version_command>
+  <command detect_errors="exit_code"><![CDATA[
+    Rscript '${__tool_directory__}/phyloseq_net.r'
+    #if str($file_source.file_source_selector) == "set_biom":
+        --infile='$input' 
+        --metafile='$metadata_input' 
+        --biom="${file_source.file_source_selector}" 
+        --xcolumn="${xaxis_column}" 
+        --lcolumn="${legend_column}" 
+        --outdir="$htmlfile.files_path" 
+        --htmlfile='$htmlfile'
+    #else:
+        --infile='$input' 
+        --metafile='$metadata_input' 
+        --biom="${file_source.file_source_selector}" 
+        --obsfile="${file_source.obstable}" 
+        --norm="${file_source.norm_bool}" 
+        --xcolumn="${xaxis_column}" 
+        --lcolumn="${legend_column}" 
+        --outdir="$htmlfile.files_path" 
+        --htmlfile='$htmlfile'
+    #end if
+  ]]></command>
+ 
+  <inputs>
+
+    <conditional name="file_source">
+      <param name="file_source_selector" type="select" label="Choose a file type">
+	<option value="set_biom" selected="True"> BIOM File</option>
+	<option value="set_table"> Count File </option>
+      </param>
+      <when value="set_biom">
+	 <param format="biom1" name="input" type="data" label="Input File"/>
+
+      </when>
+      <when value="set_table">
+	<param format="tabular" name="input" type="data" label="Input File"/>
+	<param format="tabular" name="obstable" type="data" label="Observation file"/>
+	<param name="norm_bool" type="boolean" truevalue="true" falsevalue="false" checked="false" label="is the data normalised?"/>
+      </when>
+    </conditional>
+    <param format="tabular" name="metadata_input" type="data" label="Metadata file"/>
+    <param name="xaxis_column" type="data_column" data_ref="metadata_input" use_header_names="TRUE" label="Select a group for distance matrix"/>
+    <param name="legend_column" type="data_column" data_ref="metadata_input" use_header_names="TRUE" label="Column used as legend"/>
+  </inputs>
+ 
+  <outputs>
+        <data format="html" name="htmlfile" label="${tool.name}.html"/>
+  </outputs>
+
+  <tests>
+  	 <test>  <!-- Test #1: Test BIOM format input -->
+	 		<!-- <conditional name="file_source"> -->
+				<param name="file_source_selector" value="set_biom"/>
+				<param name="input" value="GP.biom" ftype="biom1" />
+				<param name="norm_bool" value="true"/>
+				<param name="metadata_input" value="metadata.txt" ftype="tabular"/>
+				<param name="xaxis_column" value="5" />
+				<param name="legend_column" value="4" />
+			<!-- </conditional>-->
+			 <output name="htmlfile" ftype="html" file="biom_out.html" />
+	 </test>
+	 <test>  <!-- Test #2: Test TABULAR format inputs -->
+	 		<!-- <conditional name="file_source"> -->
+				 <param name="file_source_selector" value="set_table" />
+				 <param name="input" value="count.txt" ftype="tabular" />
+				 <param name="obstable" value="observation.txt" ftype="tabular" />
+				 <param name="metadata_input" value="metadata.txt" ftype="tabular" />
+				 <param name="norm_bool" value="true" />
+				 <param name="xaxis_column" value="5" />
+				 <param name="legend_column" value="4" />
+			<!-- </conditional> -->
+			<output name="htmlfile" ftype="html" file="test.html" />
+	 </test>
+  </tests>
+
+  <help>
+**What it does**
+Creates a correlation network plot between samples using an R package called phyloseq_.
+
+.. _phyloseq: https://joey711.github.io/phyloseq/plot_network-examples.html
+
+-----
+
+**Input**
+
+
+- **Choose a file type** - choose the input file type (e.g., BIOM or count table)
+- **Input file** - choose the input file
+- **Observation** - appear only if the "count table" selected in "choose a file type"
+- **is the data normalised** - appear only if the "count table" selected in "choose a file type"
+- **Select a group for distance matrix** - This group will be used to calculate the distance matrix
+- **Column used as legend** - select a group from the metadata for legend
+
+
+.. _BIOM: http://biom-format.org/
+
+-----
+=========
+Resources
+=========
+
+**Wrapper Authors**
+
+QFAB Bioinformatics (support@qfab.org)
+
+  </help>
+  <citations>
+	 <citation type="doi">10.18129/B9.bioc.phyloseq</citation>
+	 <citation type="dio">doi:10.1186/2047-217X-1-7</citation>
+  </citations>
+
+</tool>
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/GP.biom	Mon Sep 14 08:13:43 2020 +0000
@@ -0,0 +1,1 @@
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/biom_out.html	Mon Sep 14 08:13:43 2020 +0000
@@ -0,0 +1,13 @@
+<html><body>
+<table align="center">
+<tr>
+<td valign="middle" style="vertical-align:middle;">
+<<<<<<< HEAD
+<a href="pdffile.pdf"><img src="net.png"/></a>
+=======
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+>>>>>>> 7071f56839dc493d472263deb5bf5bf3e3909674
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+</tr>
+</table>
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/count.txt	Mon Sep 14 08:13:43 2020 +0000
@@ -0,0 +1,3359 @@
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/metadata.txt	Mon Sep 14 08:13:43 2020 +0000
@@ -0,0 +1,19 @@
+#SampleID	dpw	time	Food	Replicate_Group
+F3D0	0	Early	None	Group1
+F3D1	1	Early	None	Group1
+F3D2	2	Early	None	Group1
+F3D3	3	Early	Cheddar	Group2
+F3D5	5	Early	Cheddar	Group2
+F3D6	6	Early	Cheddar	Group2
+F3D7	7	Early	Swiss	Group3
+F3D8	8	Early	Swiss	Group3
+F3D9	9	Early	Swiss	Group3
+F3D141	141	Late	Cheddar	Group4
+F3D142	142	Late	Cheddar	Group4
+F3D143	143	Late	Cheddar	Group4
+F3D144	144	Late	None	Group5
+F3D145	145	Late	None	Group5
+F3D146	146	Late	None	Group5
+F3D147	147	Late	Swiss	Group6
+F3D148	148	Late	Swiss	Group6
+F3D149	149	Late	Swiss	Group6
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/observation.txt	Mon Sep 14 08:13:43 2020 +0000
@@ -0,0 +1,3359 @@
+OTUID	taxonomy
+775126	k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__; g__; s__
+228502	k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__Ruminococcaceae; g__Ruminococcus; s__
+314713	k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__Ruminococcaceae; g__Ruminococcus; s__
+314425	k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__Ruminococcaceae; g__Ruminococcus; s__
+316122	k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__; g__; s__
+432015	k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__; g__; s__
+4374047	k__Bacteria; p__Actinobacteria; c__Coriobacteriia; o__Coriobacteriales; f__Coriobacteriaceae; g__Adlercreutzia; s__
+344726	k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__Lachnospiraceae; g__; s__
+1078157	k__Bacteria; p__Firmicutes; c__Bacilli; o__Bacillales; f__Staphylococcaceae; g__Staphylococcus; s__
+974097	k__Bacteria; p__Firmicutes; c__Bacilli; o__Bacillales; f__Staphylococcaceae; g__Staphylococcus; s__
+323154	k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__Lachnospiraceae; g__; s__
+266555	k__Bacteria; p__Actinobacteria; c__Coriobacteriia; o__Coriobacteriales; f__Coriobacteriaceae; g__Adlercreutzia; s__
+4316029	k__Bacteria; p__Firmicutes; c__Bacilli; o__Bacillales; f__Bacillaceae; g__; s__
+317867	k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__; g__; s__
+190425	k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__; g__; s__
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+271499	k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__; g__; s__
+338644	k__Bacteria; p__Actinobacteria; c__Coriobacteriia; o__Coriobacteriales; f__Coriobacteriaceae; g__Adlercreutzia; s__
+338640	k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__Ruminococcaceae; g__Ruminococcus; s__
+313804	k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__Lachnospiraceae; g__; s__
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+351343	k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__Lachnospiraceae; g__[Ruminococcus]; s__gnavus
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+339092	k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__Lachnospiraceae; g__[Ruminococcus]; s__gnavus
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+162485	k__Bacteria; p__Firmicutes; c__Clostridia; o__Clostridiales; f__Lachnospiraceae; g__; s__
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/test.html	Mon Sep 14 08:13:43 2020 +0000
@@ -0,0 +1,9 @@
+<html><body>
+<table align="center">
+<tr>
+<td valign="middle" style="vertical-align:middle;">
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