annotate rcas.xml @ 0:aa9579837a2e draft

planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
author rnateam
date Thu, 13 Apr 2017 12:36:05 -0400
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children 7c7a2a381dfe
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aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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1 <?xml version="1.0" encoding="UTF-8"?>
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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2 <tool id="rcas" name="RNA Centric Annotation System" version="1.1.1">
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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3 <requirements>
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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4 <requirement type="package" version="1.1.1">bioconductor-rcas</requirement>
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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5 </requirements>
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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6 <stdio>
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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7 <regex match="Execution halted" source="both" level="fatal" description="Execution halted." />
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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8 <exit_code range="1:" />
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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9 </stdio>
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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10 <command><![CDATA[Rscript '$script_file'
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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11
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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12 &&
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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13 mv *RCAS.report.html RCAS.report.html
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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14
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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16 #if $run_anot == "TRUE":
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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17 &&
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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18 mv Figure*summarizeQueryRegions.data.tsv summarizeQueryRegions.data.tsv &&
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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19 mv Figure*query_gene_types.data.tsv query_gene_types.data.tsv &&
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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20 mv Figure*transcriptBoundaryCoverage.fiveprime.data.tsv transcriptBoundaryCoverage.fiveprime.data.tsv &&
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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21 mv Figure*transcriptBoundaryCoverage.threeprime.data.tsv transcriptBoundaryCoverage.threeprime.data.tsv &&
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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22 mv Figure*exonIntronBoundaryCoverage.fiveprime.data.tsv exonIntronBoundaryCoverage.fiveprime.data.tsv &&
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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23 mv Figure*exonIntronBoundaryCoverage.threeprime.data.tsv exonIntronBoundaryCoverage.threeprime.data.tsv &&
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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24 mv Figure*coverageprofilelist.data.tsv coverageprofilelist.data.tsv &&
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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25 mv Table*getTargetedGenesTable.data.tsv getTargetedGenesTable.data.tsv
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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26 #end if
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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27
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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28
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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29 #if $run_GOrich == "TRUE":
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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30 &&
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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31 mv Table*goBP.data.tsv goBP.data.tsv &&
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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32 mv Table*goMF.data.tsv goMF.data.tsv &&
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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33 mv Table*goCC.data.tsv goCC.data.tsv
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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34 #end if
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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35
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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36
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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37 #if $choice.run_PATHrich == "TRUE":
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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38 &&
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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39 mv Table*GSEA.data.tsv GSEA.data.tsv
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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40 #end if
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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41
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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42
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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43 #if $run_motif == "TRUE":
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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44 &&
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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45 mv Table*motif_analysis_table.data.tsv motif_analysis_table.data.tsv
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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46 #end if]]></command>
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47 <configfiles>
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48 <configfile name="script_file">library("RCAS")
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49
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50 runReport(queryFilePath = '${input_BED}',
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51
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52 gffFilePath = '${input_GTF}',
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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53
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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54 #if $choice.run_PATHrich == "TRUE":
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55 msigdbFilePath = '${choice.input_human_msigdb_gmt}',
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56 #end if
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57
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58 annotationSummary = ${run_anot},
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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59
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60 goAnalysis = ${run_GOrich},
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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61
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62 msigdbAnalysis = ${choice.run_PATHrich},
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63
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64 motifAnalysis = ${run_motif},
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65
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66 genomeVersion = '${genomeVersion}',
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67
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68 outDir = getwd(),
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69
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70 printProcessedTables = TRUE,
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71
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72 sampleN = '${downsampling}',
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73
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74 selfContained = TRUE)</configfile>
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75 </configfiles>
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76 <inputs>
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77 <param name="genomeVersion" type="select" label="Genome Version">
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78 <option value="hg19" selected="true">hg19</option>
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79 <option value="dm3">dm3</option>
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80 <option value="ce10">ce10</option>
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81 <option value="mm9">mm9</option>
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82 </param>
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83 <param name="input_BED" type="data" format="BED" label="Target regions in BED format" help="The coordinates should be in accord with Genome Version." />
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84 <param name="input_GTF" type="data" format="GTF" label="Reference annotation in ENSEMBL GTF format" />
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85 <param name="run_anot" label="Run annotation." type="boolean" falsevalue="FALSE" truevalue="TRUE" checked="True" help="Use this option if you want to run annotation." />
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86 <param name="run_GOrich" label="Run GO term enrichment" type="boolean" falsevalue="FALSE" truevalue="TRUE" checked="False" help="Use this option if you want to run GO term enrichment (supported genome versions: hg19, mm9, dm3)." />
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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87 <conditional name="choice">
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88 <param name="run_PATHrich" type="select" label="Run gene set enrichment" help="Use this option if you want to run gene set enrichment.">
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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89 <option value="FALSE" selected="True">No</option>
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90 <option value="TRUE">Yes</option>
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91 </param>
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92 <when value="TRUE">
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93 <param name="input_human_msigdb_gmt" type="data" format="tabular" label="Human Molecular Signatures Database (MSigDB)" help="The database is usesd for gene set enrichment (supported genome versions: hg19, mm9, dm3). For non-human species, the human MSigDB will be automatically converted accordingly." />
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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94 </when>
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95 <when value="FALSE" />
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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96 </conditional>
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97 <param name="run_motif" label="Run motif search" type="boolean" falsevalue="FALSE" truevalue="TRUE" checked="False" help="Use this option if you want to run motif search." />
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98 <param name="downsampling" label="Downsampling (N)" type="text" value="0" help="Randomly sample query regions down to (N)" />
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99 </inputs>
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100 <outputs>
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101 <data name="report" format="html" from_work_dir="RCAS.report.html" label="${tool.name} on ${on_string}: dynamic HTML" />
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102 <data name="summarizeQueryRegions" format="tsv" from_work_dir="summarizeQueryRegions.data.tsv" label="${tool.name} on ${on_string}: Query regions summary">
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103 <filter>run_anot == True</filter>
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104 </data>
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105 <data name="query_gene_types" format="tsv" from_work_dir="query_gene_types.data.tsv" label="${tool.name} on ${on_string}: Query gene types">
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106 <filter>run_anot == True</filter>
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107 </data>
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108 <data name="transcriptBoundaryCoverage.fiveprime" format="tsv" from_work_dir="transcriptBoundaryCoverage.fiveprime.data.tsv" label="${tool.name} on ${on_string}: Transcript boundary coverage (5')">
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109 <filter>run_anot == True</filter>
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110 </data>
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111 <data name="transcriptBoundaryCoverage.threeprime" format="tsv" from_work_dir="transcriptBoundaryCoverage.threeprime.data.tsv" label="${tool.name} on ${on_string}: Transcript boundary coverage (3')">
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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112 <filter>run_anot == True</filter>
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113 </data>
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114 <data name="exonIntronBoundaryCoverage.fiveprime" format="tsv" from_work_dir="exonIntronBoundaryCoverage.fiveprime.data.tsv" label="${tool.name} on ${on_string}: Exon-intron boundary coverage (5')">
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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115 <filter>run_anot == True</filter>
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116 </data>
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117 <data name="exonIntronBoundaryCoverage.threeprime" format="tsv" from_work_dir="exonIntronBoundaryCoverage.threeprime.data.tsv" label="${tool.name} on ${on_string}: Exon-intron boundary coverage (3')">
aa9579837a2e planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rcas/ commit 3b9aec9beb72407e01f4a0c5b31c17ac3f92a851
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118 <filter>run_anot == True</filter>
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119 </data>
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120 <data name="coverageprofilelist" format="tsv" from_work_dir="coverageprofilelist.data.tsv" label="${tool.name} on ${on_string}: Coverage profile list">
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121 <filter>run_anot == True</filter>
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122 </data>
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123 <data name="getTargetedGenesTable" format="tsv" from_work_dir="getTargetedGenesTable.data.tsv" label="${tool.name} on ${on_string}: Targeted genes">
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124 <filter>run_anot == True</filter>
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125 </data>
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126 <data name="goCC" format="tsv" from_work_dir="goCC.data.tsv" label="${tool.name} on ${on_string}: GO term enrichment (cellular compartments)">
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127 <filter>run_GOrich == True</filter>
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128 </data>
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129 <data name="goBP" format="tsv" from_work_dir="goBP.data.tsv" label="${tool.name} on ${on_string}: GO term enrichment (biological processes)">
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130 <filter>run_GOrich == True</filter>
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131 </data>
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132 <data name="goMF" format="tsv" from_work_dir="goMF.data.tsv" label="${tool.name} on ${on_string}: GO term enrichment (molecular functions)">
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133 <filter>run_GOrich == True</filter>
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134 </data>
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135 <data name="GSEA" format="tsv" from_work_dir="GSEA.data.tsv" label="${tool.name} on ${on_string}: Gene set enrichment analysis">
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136 <filter>choice['run_PATHrich'] == "TRUE"</filter>
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137 </data>
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138 <data name="motif" format="tsv" from_work_dir="motif_analysis_table.data.tsv" label="${tool.name} on ${on_string}: Motif">
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139 <filter>run_motif == True</filter>
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140 </data>
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141 </outputs>
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142 <tests>
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143 <test>
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144 <param name="input_BED" value="input.TIA1.bed" />
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145 <param name="input_GTF" value="input.Homo_sapiens.GRCh37-chr1-f10k.75.gtf" />
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146 <param name="input_human_msigdb_gmt" value="input.msigdb_test.gmt" />
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147 <param name="run_anot" value="TRUE" />
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148 <param name="run_GOrich" value="TRUE" />
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149 <param name="run_PATHrich" value="TRUE" />
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150 <param name="run_motif" value="TRUE" />
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151 <param name="genomeVersion" value="hg19" />
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152 <output name="report" file="output.TIA1.bed.RCAS.report.html" ftype="html" compare="sim_size" />
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153 <output name="summarizeQueryRegions" file="summarizeQueryRegions.data.tsv" ftype="tsv" />
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154 <output name="query_gene_types" file="query_gene_types.data.tsv" ftype="tsv" />
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155 <output name="transcriptBoundaryCoverage.fiveprime" file="transcriptBoundaryCoverage.fiveprime.data.tsv" ftype="tsv" />
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156 <output name="transcriptBoundaryCoverage.threeprime" file="transcriptBoundaryCoverage.threeprime.data.tsv" ftype="tsv" />
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157 <output name="exonIntronBoundaryCoverage.fiveprime" file="exonIntronBoundaryCoverage.fiveprime.data.tsv" ftype="tsv" />
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158 <output name="exonIntronBoundaryCoverage.threeprime" file="exonIntronBoundaryCoverage.threeprime.data.tsv" ftype="tsv" />
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159 <output name="coverageprofilelist" file="coverageprofilelist.data.tsv" ftype="tsv" />
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160 <output name="getTargetedGenesTable" file="getTargetedGenesTable.data.tsv" ftype="tsv" />
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161 <output name="goCC" file="goCC.data.tsv" ftype="tsv" compare="sim_size"/>
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162 <output name="goBP" file="goBP.data.tsv" ftype="tsv" compare="sim_size"/>
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163 <output name="goMF" file="goMF.data.tsv" ftype="tsv" compare="sim_size"/>
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164 <output name="GSEA" file="GSEA.data.tsv" ftype="tsv" />
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165 <output name="motif" file="motif_analysis_table.data.tsv" ftype="tsv" compare="sim_size" />
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166 </test>
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167 </tests>
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168 <help><![CDATA[.. class:: infomark
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169
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170 **Purpose**
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171
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172 RNA Centric Annotation System (RCAS) automatically generates
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173 dynamic annotations for custom input files
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174 that contain transcriptomic target regions.
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175 RCAS automatically summarizes annotated features
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176 that overlap with targets in the BED file,
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177 and perform enrichment analysis of
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178 Gene Ontology (GO) terms, gene set and motifs.
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179
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180 -----
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181
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182 .. class:: infomark
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183
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184 **Inputs**
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185
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186 1. A target region file in BED format
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187 2. A reference annotation file in GTF format
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188 3. A Human Molecular Signatures Database (MSigDB) (only needed for gene set enrichment)
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189
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190 -----
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191
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192 .. class:: infomark
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193
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194 **Outputs**
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195
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196 The outputs consist of a dynamic HTML file and
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197 a dozen of tabular files.
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198 The dynamic HTML file is composed of the interactive tables and figures,
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199 which can be downloaded and viewed with a web browser.
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200
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201 The tabular files correspond to the respective figures in the HTML file,
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202 which are the results
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203 of different RCAS analysis, as following:
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204
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205 1. Annotation summary for query regions
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206
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207 * Query regions summary
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208 * Query gene types
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209 * Transcript boundary coverage (5')
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210 * Transcript boundary coverage (3')
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211 * Exon-intron boundary coverage (5')
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212 * Exon-intron boundary coverage (3')
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213 * Coverage profile list
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214 * Targeted genes
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215
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216 2. GO term analysis results
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217
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218 * GO term enrichment (cellular compartments)
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219 * GO term enrichment (biological processes)
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220 * GO term enrichment (molecular functions)
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221
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222 3. Gene set enrichment analysis results
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223
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224 4. Motif analysis results]]></help>
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225 <citations>
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226 <citation type="doi">10.1093/nar/gkx120</citation>
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227 </citations>
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228 </tool>