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author | siyuan |
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date | Thu, 20 Feb 2014 00:44:58 -0500 |
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PRADA: Pipeline for RnAseq Data Analysis AUTHOR: Siyuan Zheng, Wandaliz-Torres Garcia, Rahul Vegesna CONTACT: Siyuan Zheng (szheng2@mdanderson.org), Roel Verhaak (rverhaak@mdanderson.org) COPY RIGHT: Verhaak Lab,Department of Bioinformatics and Computational Biology The University of Texas MD Anderson Cancer Center VERSION: 1.2 DATE: 02/16/2014 REQUIRE: Python v2.6 or v2.7; Python Libs: pysam, Biopython PLATFORM: Linux, tested with PBS, B-shell and TC-shell CITATION PRADA: Pipeline for RNA sequencing Data Analysis Bioinformatics. Coming soon. TOOLS INCLUDED: prada-fusion genome-widely identify gene fusions prada-preprocess preprocess paired end RNAseq data prada-frame predict functional consequences of gene fusions prada-homology calculate gene sequence similarity prada-guess-ft supervised search of a gene fusion prada-guess-if supervised search of intragenic fusions (normal splicing excluded) WHAT'S NEW 1. New configur allows more user customization to bwa alignment and PBS script. 2. Reorganized preprocess module for better control of BAM an FASTQ as input. 3. A test data set is added.