2
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
1 <tool id="NFR" name="Nucleosome Free Regions" version="0.1">
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
2 <description>: determine Nucleosome Free Regions from nucleR nucleosome calls</description>
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
3 <requirements>
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
4 <requirement type="binary">docker</requirement>
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
5 </requirements>
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
6 <command>
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
7 <![CDATA[
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
8 docker run -v $__root_dir__/database/files:$__root_dir__/database/files -v /tmp:/tmp -u `id -u`:`id -g` mmbirb/nucleosome-dynamics NFR --input $gff_file --output $output_gff_file --minwidth $minwidth --threshold $threshold
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
9 ]]>
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
10 </command>
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
11 <inputs>
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
12 <param name="gff_file" type="data" format="gff" label="Nucleosome Calls (GFF)" help="Nucleosome calls in GFF format as obtained from nucleR."/>
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
13 <param name="minwidth" size="4" type="integer" value="110" label="Minimum Width" help="Minimum width for a linker fragment to be considered a nucleosome-free region." />
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
14 <param name="threshold" size="4" type="integer" value="140" label="Maximum Width" help="Maximum width for a linker fragment to be considered a nucleosome-free region." />
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
15 </inputs>
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
16 <outputs>
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
17 <data format="gff" name="output_gff_file" label="NFR__${os.path.splitext(($gff_file.name.split('__'))[1])[0]}.gff" />
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
18 </outputs>
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
19 <tests>
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
20 <test>
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
21 <param name="gff_file" value="NR__cellcycleM_chrII.gff"/>
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
22 <param name="minwidth" value="110" />
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
23 <param name="threshold" value="400" />
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
24 <output name="output_gff_file" file="NFR__cellcycleM_chrII.gff" />
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
25 </test>
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
26 </tests>
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
27 <help>
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
28 .. image:: ${static_path}/images/NucleosomeDynamicsLogo.png
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
29 :height: 80
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
30 :width: 200
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
31
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
32 -----
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
33
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
34 Nucleosome Dynamics is a set of tools that take MNase-seq and ATAC-seq aligned reads and performs a serie of nucleosome-related analyses on them.
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
35
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
36 .. class:: infomark
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
37
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
38 Visit the documentation of the original application for learning more about the accepted values and formats. http://mmb.irbbarcelona.org/NucleosomeDynamics/help/usage/nucleosome-dynamics
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
39 </help>
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
40 <citations>
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
41 <citation type="bibtex">
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
42 @misc{github,
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
43 author = {Buitrago D},
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
44 year = {2019},
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
45 title = {Nucleosome Dynamics suite: containerized installation},
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
46 publisher = {GitHub},
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
47 journal = {GitHub repository},
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
48 url = {https://github.com/nucleosome-dynamics/nucleosome_dynamics},
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
49 }</citation>
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
50 </citations>
|
spanish_national_institue_of_bioinformatics
parents:
diff
changeset
|
51 </tool>
|