Mercurial > repos > tduigou > rpbasicdesign
annotate rpbasicdesign.xml @ 6:ce36e195a61b draft
planemo upload for repository https://github.com/brsynth/rpbasicdesign commit 2e8aa2e7c0581c7eed2074d15f7e4317ecde2ad6
author | tduigou |
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date | Mon, 01 May 2023 01:25:39 +0000 |
parents | e743b6118dae |
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1 <tool id="rpbasicdesign" name="BasicDesign" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="21.09"> |
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2 <description>Build DNA-BOT input files from rpSBML</description> |
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3 <macros> |
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4 <token name="@VERSION_SUFFIX@">0</token> |
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5 <token name="@TOOL_VERSION@">1.2.2</token> |
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6 </macros> |
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7 <requirements> |
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8 <requirement type="package" version="@TOOL_VERSION@">rpbasicdesign</requirement> |
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9 </requirements> |
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10 <command detect_errors="exit_code"><![CDATA[ |
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11 python -m rpbasicdesign.cli |
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12 --rpsbml_file '$rpsbml_file' |
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13 #if $adv.parts_files |
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14 #set files = '" "'.join([str($file) for $file in $adv.parts_files]) |
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15 --parts_files "${files}" |
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16 #end if |
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17 --lms_id '$adv.lms_id' |
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18 --lmp_id '$adv.lmp_id' |
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19 --backbone_id '$backbone_id' |
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20 --sample_size '$sample_size' |
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21 $adv.cds_permutation |
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22 --o_dnabot_dir 'out/dnabot_in' |
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23 $adv.sbol_output |
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24 --max_enz_per_rxn $adv.max_enz_per_rxn |
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25 --max_gene_per_construct $adv.max_gene_per_construct |
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26 ]]></command> |
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27 <inputs> |
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28 <param name="rpsbml_file" type="data" format="sbml" label="rpSBML file" help="SBML file from which enzymes UniProt IDs will be collected."/> |
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29 <param argument="--backbone_id" type="text" value="BASIC_SEVA_37_CmR-p15A.1" label="Backbone part ID" help="Part ID to be used as the backbone."> |
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30 <sanitizer invalid_char=""> |
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31 <valid initial="string.letters,string.digits"> |
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32 <add value=":" /> |
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33 <add value="." /> |
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34 <add value="-" /> |
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35 <add value="_" /> |
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36 </valid> |
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37 </sanitizer> |
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38 <validator type="empty_field" message="Backbone ID is required"/> |
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39 </param> |
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40 <param argument="--sample_size" type="integer" value="88" min="1" max="88" label="Sample size" help="Number of construct to generate."/> |
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41 <section name="adv" title="Advanced Options" expanded="false"> |
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42 <param name="parts_files" type="data" format="csv" optional="true" multiple="true" label="Linkers and user parts" help="List of files providing available linkers and user parts (backbone, promoters, ...) for constructs. Default: Standard Biolegio Parts (BBP-18500)" /> |
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43 <param argument="--lms_id" type="text" value="LMS" label="LMS part ID" help="Part ID to be used as the LMS methylated linker." > |
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44 <sanitizer invalid_char=""> |
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45 <valid initial="string.letters,string.digits"> |
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46 <add value=":" /> |
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47 <add value="-" /> |
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48 <add value="_" /> |
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49 <add value="." /> |
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50 </valid> |
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51 </sanitizer> |
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52 <validator type="empty_field" message="LMS ID is required"/> |
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53 </param> |
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54 <param argument="--lmp_id" type="text" value="LMP" label="LMP part ID" help="Part ID to be used as the LMP methylated linker."> |
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55 <sanitizer invalid_char=""> |
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56 <valid initial="string.letters,string.digits"> |
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57 <add value=":" /> |
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58 <add value="-" /> |
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59 <add value="_" /> |
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60 <add value="." /> |
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61 </valid> |
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62 </sanitizer> |
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63 <validator type="empty_field" message="LMP ID is required"/> |
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64 </param> |
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65 <param argument="--cds_permutation" type="boolean" truevalue="--cds_permutation true" falsevalue="--cds_permutation false" checked="true" label="Perform CDS permutation?" help="Whether all combinations of CDS permutation should be built." /> |
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66 <param argument="--sbol_output" type="boolean" checked="false" truevalue="--o_sbol_dir out/sbol_export" falsevalue="" label="Output SBOL results?" help="Whether SBOL (Synthetic Biology Open Language) depictions of constructs should be outputted" /> |
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67 <param argument="--max_enz_per_rxn" type="integer" value="1" min="1" max="99" label="Maximum number of enyzme to consider per reaction." help="Maximum number of enyzme to consider per reaction. If more enzymes are available for a given reaction, then only the last one listed in the MIRIAM annotation section will be kept."/> |
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68 <param argument="--max_gene_per_construct" type="integer" value="3" min="1" max="10" label="Maximum number of genes per construct" help="If more genes are required, i.e. more reactions are described in the input SBML file, then the execution will failed."/> |
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69 </section> |
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70 </inputs> |
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71 <outputs> |
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72 <data name="Constructs" format="csv" from_work_dir="out/dnabot_in/constructs.csv" label="${tool.name} on ${rpsbml_file.name}: constructs" /> |
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73 <data name="User_parts_plate" format="csv" from_work_dir="out/dnabot_in/user_parts_plate.csv" label="${tool.name} on ${rpsbml_file.name}: User parts plate"/> |
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74 <data name="Biolegio_plate" format="csv" from_work_dir="out/dnabot_in/biolegio_plate.csv" label="${tool.name} on ${rpsbml_file.name}: Biolegio plate"/> |
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75 <collection name="sbol_dir" type="list" label="${tool.name} on ${rpsbml_file.name}: SBOL constructs"> |
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76 <filter> adv['sbol_output'] </filter> |
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77 <discover_datasets pattern="__designation_and_ext__" format="xml" directory="out/sbol_export" /> |
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78 </collection> |
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79 </outputs> |
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80 <tests> |
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81 <test expect_num_outputs="4"> |
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82 <!-- test 1: check if identical outputs are produced (Lycopene input)--> |
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83 <param name="rpsbml_file" value="lycopene_CrtEBI_from_selenzy.xml" /> |
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84 <param name="sample_size" value="3" /> |
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85 <output name="Constructs" file="constructs_lycopene.csv" ftype="csv" compare="diff"> |
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86 <assert_contents> |
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87 <has_n_lines n="4"/> |
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88 </assert_contents> |
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89 </output> |
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90 <output name="User_parts_plate" file="user_parts_plate_lycopene.csv" ftype="csv" compare="diff"/> |
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91 <output name="Biolegio_plate" file="biolegio_plate_lycopene.csv" ftype="csv" compare="diff"/> |
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92 <param name="sbol_output" value="true" /> |
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93 <output_collection name="sbol_dir" type="list" count="3"> |
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94 <element name="BASIC_construct_A1"> |
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95 <assert_contents> |
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96 <is_valid_xml /> |
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97 <has_text text="BASIC_construct_A1" /> |
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98 <has_n_lines n="339" /> |
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99 </assert_contents> |
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100 </element> |
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101 <element name="BASIC_construct_B1"> |
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102 <assert_contents> |
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103 <is_valid_xml /> |
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104 <has_text text="BASIC_construct_B1" /> |
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105 <has_n_lines n="339" /> |
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106 </assert_contents> |
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107 </element> |
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108 <element name="BASIC_construct_C1"> |
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109 <assert_contents> |
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110 <is_valid_xml /> |
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111 <has_text text="BASIC_construct_C1" /> |
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112 <has_n_lines n="339" /> |
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113 </assert_contents> |
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114 </element> |
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115 </output_collection> |
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116 </test> |
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117 <test expect_num_outputs="3"> |
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118 <!-- test 2: check if identical outputs are produced (Muconate input)--> |
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119 <param name="rpsbml_file" value="muconate_example.xml" /> |
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120 <param name="sample_size" value="88" /> |
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121 <output name="Constructs" file="constructs_muconate.csv" ftype="csv" compare="diff"> |
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122 <assert_contents> |
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123 <has_n_lines n="89"/> |
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124 </assert_contents> |
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125 </output> |
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126 <output name="User_parts_plate" file="user_parts_plate_muconate.csv" ftype="csv" compare="diff"/> |
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127 <output name="Biolegio_plate" file="biolegio_plate_muconate.csv" ftype="csv" compare="diff"/> |
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128 </test> |
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129 </tests> |
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130 <help><![CDATA[ |
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131 rpbasicdesign |
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132 ================ |
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133 rpbasicdesign extracts enzyme IDs from rpSBML (System Biology Markup Language) files containing additionnal annotations (e.g. reaction rules ID) and produced by the RP (RetroPath) suite available in `SynBioCAD Galaxy platform <https://galaxy-synbiocad.org/>`_, to generate genetic constructs compliant with the BASIC (Biopart Assembly Standard for Idempotent Cloning) assembly approach. CSV files produced are ready to be used with DNA-Bot to generate instructions for automated build of the genetic constructs using OpenTrons liquid handling robots. |
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134 Input |
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135 ----- |
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136 Required: |
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137 * **rpSBML file**\ : rpSBML file from which enzymes UniProt IDs will be collected. |
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138 Advanced options: |
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139 * **Linkers and user parts**\ : (string) List of files providing available linkers and user parts (backbone, promoters, ...) for constructs. Default: ( `Standard Biolegio Parts <https://www.biolegio.com/>`_: BBP-18500). |
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140 * **LMS part ID**\ : (string) part ID to be used as the LMS methylated linker. Default: LMS. |
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141 * **LMP part ID**\ : (string) part ID to be used as the LMP methylated linker. Default: LMP. |
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142 * **Backbone part ID**\ : (string) part ID to be used as the backbone. Default: BASIC_SEVA_37_CmR-p15A.1. |
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143 * **Sample size**\ : (int) Number of construct to generate. Default: 88. |
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144 * **Perform CDS permutation?**\ : (boolean) Whether all combinations of CDS permutation should be built Default: true. |
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145 * **Maximum number of enyzme to consider per reaction**\ : (int) Maximum number of enyzme to consider per reaction. If more enzymes are available for a given reaction, then only the last one listed in the MIRIAM annotation section will be kept. (Default: 1). |
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146 * **Maximum number of genes per construct**\ : (int) If more genes are required, i.e. more reactions are described in the input SBML file, then the execution will failed. (Default: 3). |
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147 Output |
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148 ------ |
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149 * **constructs**\ : CSV construct file listing the constructs to be built. |
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150 * **User parts plate**\ : CSV file listing the DNA parts to be included into each construct. |
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151 * **Biolegio plate**\ : CSV file listing the plate coordinates of the BASIC linkers. |
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152 * **SBOL constructs**\ : (optional) one SBOL (Synthetic Biology Open Language) file is produced for each construct generated in XML format. |
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153 Project Links |
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154 ------------------ |
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155 * `GitHub <https://github.com/brsynth/rpbasicdesign>`_ |
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156 License |
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157 ------- |
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158 * `MIT <https://github.com/brsynth/rpbasicdesign/blob/master/LICENSE.txt>`_ |
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159 ]]></help> |
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160 <citations> |
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161 <citation type="doi">10.1093/synbio/ysaa010</citation> |
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162 </citations> |
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163 </tool> |