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1 package edu.unc.genomics.wigmath;
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2
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3 import java.io.BufferedWriter;
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4 import java.io.IOException;
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5 import java.nio.charset.Charset;
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6 import java.nio.file.Files;
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7 import java.nio.file.Path;
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8 import java.util.ArrayList;
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9 import java.util.HashSet;
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10 import java.util.Iterator;
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11 import java.util.List;
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12 import java.util.Set;
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13
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14 import org.apache.log4j.Logger;
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15
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16 import com.beust.jcommander.Parameter;
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17
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18 import edu.unc.genomics.CommandLineTool;
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19 import edu.unc.genomics.io.WigFile;
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20 import edu.unc.genomics.io.WigFileException;
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21
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22 /**
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23 * Abstract class for writing programs to do computation on Wig files
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24 * Concrete subclasses must implement the compute method
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25 *
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26 * @author timpalpant
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27 *
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28 */
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29 public abstract class WigMathTool extends CommandLineTool {
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30
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31 private static final Logger log = Logger.getLogger(WigMathTool.class);
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32
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33 public static final int DEFAULT_CHUNK_SIZE = 500_000;
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34
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35 // TODO: Variable resolution output
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36
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37 @Parameter(names = {"-o", "--output"}, description = "Output file", required = true)
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38 public Path outputFile;
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39
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40 protected List<WigFile> inputs = new ArrayList<WigFile>();
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41
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42 public void addInputFile(WigFile wig) {
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43 inputs.add(wig);
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44 }
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45
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46 /**
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47 * Setup the computation. Should add all input Wig files
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48 * with addInputFile() during setup
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49 */
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50 public abstract void setup();
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51
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52 /**
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53 * Do the computation on a chunk and return the results
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54 * Must return (stop-start+1) values
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55 *
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56 * @param chr
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57 * @param start
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58 * @param stop
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59 * @return the results of the computation for this chunk
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60 * @throws IOException
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61 * @throws WigFileException
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62 */
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63 public abstract float[] compute(String chr, int start, int stop)
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64 throws IOException, WigFileException;
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65
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66 @Override
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67 public void run() throws IOException {
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68 log.debug("Executing setup operations");
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69 setup();
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70
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71 log.debug("Processing files and writing result to disk");
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72 try (BufferedWriter writer = Files.newBufferedWriter(outputFile, Charset.defaultCharset())) {
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73 // Write the Wig header
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74 writer.write("track type=wiggle_0");
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75 writer.newLine();
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76
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77 Set<String> chromosomes = getCommonChromosomes(inputs);
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78 log.debug("Found " + chromosomes.size() + " chromosomes in common between all inputs");
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79 for (String chr : chromosomes) {
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80 int start = getMaxChrStart(inputs, chr);
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81 int stop = getMinChrStop(inputs, chr);
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82 log.debug("Processing chromosome " + chr + " region " + start + "-" + stop);
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83
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84 // Write the chromosome header to output
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85 writer.write("fixedStep chrom="+chr+" start="+start+" step=1 span=1");
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86 writer.newLine();
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87
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88 // Process the chromosome in chunks
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89 int bp = start;
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90 while (bp < stop) {
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91 int chunkStart = bp;
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92 int chunkStop = Math.min(bp+DEFAULT_CHUNK_SIZE-1, stop);
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93 int expectedLength = chunkStop - chunkStart + 1;
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94 log.debug("Processing chunk "+chr+":"+chunkStart+"-"+chunkStop);
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95
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96 float[] result = null;
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97 try {
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98 result = compute(chr, chunkStart, chunkStop);
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99 } catch (WigFileException e) {
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100 log.fatal("Wig file error while processing chunk " + chr + " region " + start + "-" + stop);
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101 e.printStackTrace();
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102 throw new RuntimeException("Wig file error while processing chunk " + chr + " region " + start + "-" + stop);
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103 }
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104
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105 if (result.length != expectedLength) {
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106 log.error("Expected result length="+expectedLength+", got="+result.length);
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107 throw new RuntimeException("Result is not the expected length!");
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108 }
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109
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110 for (int i = 0; i < result.length; i++) {
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111 writer.write(Float.toString(result[i]));
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112 writer.newLine();
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113 }
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114
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115 bp = chunkStop + 1;
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116 }
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117 }
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118 }
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119
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120 for (WigFile wig : inputs) {
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121 wig.close();
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122 }
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123 }
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124
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125 public int getMaxChrStart(List<WigFile> wigs, String chr) {
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126 int max = -1;
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127 for (WigFile wig : wigs) {
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128 if (wig.getChrStart(chr) > max) {
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129 max = wig.getChrStart(chr);
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130 }
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131 }
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132
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133 return max;
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134 }
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135
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136 public int getMinChrStop(List<WigFile> wigs, String chr) {
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137 if (wigs.size() == 0) {
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138 return -1;
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139 }
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140
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141 int min = Integer.MAX_VALUE;
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142 for (WigFile wig : wigs) {
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143 if (wig.getChrStop(chr) < min) {
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144 min = wig.getChrStop(chr);
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145 }
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146 }
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147
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148 return min;
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149 }
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150
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151 public Set<String> getCommonChromosomes(List<WigFile> wigs) {
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152 if (wigs.size() == 0) {
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153 return new HashSet<String>();
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154 }
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155
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156 Set<String> chromosomes = wigs.get(0).chromosomes();
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157 Iterator<String> it = chromosomes.iterator();
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158 while(it.hasNext()) {
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159 String chr = it.next();
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160 for (WigFile wig : wigs) {
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161 if (!wig.includes(chr)) {
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162 it.remove();
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163 break;
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164 }
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165 }
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166 }
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167
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168 return chromosomes;
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169 }
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170 }
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