Mercurial > repos > timpalpant > java_genomics_toolkit
annotate galaxy-conf/IntervalStats.xml @ 15:3e477c7e0e73 draft
Update normalization tools to allow normalizing by chromosome (Z-scorer, scale, etc.) Add new tool to convert any interval file to Bed format. Miscellaneous bug fixes.
author | timpalpant |
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date | Sat, 09 Jun 2012 16:03:59 -0400 |
parents | 81d5b81fb3c2 |
children |
rev | line source |
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81d5b81fb3c2
Added help for all tools in the toolkit. Many bug fixes and a few new nucleosome tools.
timpalpant
parents:
4
diff
changeset
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1 <tool id="IntervalStats" name="Compute mean/min/max of intervals" version="1.0.0"> |
81d5b81fb3c2
Added help for all tools in the toolkit. Many bug fixes and a few new nucleosome tools.
timpalpant
parents:
4
diff
changeset
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2 <description>of data in a Wiggle file</description> |
4 | 3 <command interpreter="sh"> |
12
81d5b81fb3c2
Added help for all tools in the toolkit. Many bug fixes and a few new nucleosome tools.
timpalpant
parents:
4
diff
changeset
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4 galaxyToolRunner.sh ngs.IntervalStats -l $windows -s $stat -o $output |
4 | 5 #for $input in $inputs |
6 ${input.file} | |
7 #end for | |
8 </command> | |
9 <inputs> | |
10 <repeat name="inputs" title="BigWig file"> | |
11 <param name="file" type="data" format="bigwig,wig" /> | |
12 </repeat> | |
13 <param format="bed,bedgraph,gff" name="windows" type="data" label="List of intervals" /> | |
12
81d5b81fb3c2
Added help for all tools in the toolkit. Many bug fixes and a few new nucleosome tools.
timpalpant
parents:
4
diff
changeset
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14 <param name="stat" type="select" optional="true" label="For each interval, compute the"> |
4 | 15 <option value="mean">Mean</option> |
12
81d5b81fb3c2
Added help for all tools in the toolkit. Many bug fixes and a few new nucleosome tools.
timpalpant
parents:
4
diff
changeset
|
16 <!-- TODO <option value="median">Median</option> --> |
4 | 17 <option value="max">Max</option> |
18 <option value="min">Min</option> | |
12
81d5b81fb3c2
Added help for all tools in the toolkit. Many bug fixes and a few new nucleosome tools.
timpalpant
parents:
4
diff
changeset
|
19 </param> |
4 | 20 </inputs> |
21 <outputs> | |
22 <data format="tabular" name="output" /> | |
23 </outputs> | |
24 | |
25 <help> | |
12
81d5b81fb3c2
Added help for all tools in the toolkit. Many bug fixes and a few new nucleosome tools.
timpalpant
parents:
4
diff
changeset
|
26 |
81d5b81fb3c2
Added help for all tools in the toolkit. Many bug fixes and a few new nucleosome tools.
timpalpant
parents:
4
diff
changeset
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27 This tool calculates the arithmetic mean, maximum, or minimum value for the Wig data in each interval. For each Wig file provided, an additional column is added to the output file in the order that they are added above. |
81d5b81fb3c2
Added help for all tools in the toolkit. Many bug fixes and a few new nucleosome tools.
timpalpant
parents:
4
diff
changeset
|
28 |
81d5b81fb3c2
Added help for all tools in the toolkit. Many bug fixes and a few new nucleosome tools.
timpalpant
parents:
4
diff
changeset
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29 .. class:: infomark |
81d5b81fb3c2
Added help for all tools in the toolkit. Many bug fixes and a few new nucleosome tools.
timpalpant
parents:
4
diff
changeset
|
30 |
81d5b81fb3c2
Added help for all tools in the toolkit. Many bug fixes and a few new nucleosome tools.
timpalpant
parents:
4
diff
changeset
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31 **TIP:** If your dataset does not appear in the pulldown menu, it means that it is not in Wig or BigWig format. Use "edit attributes" to set the correct format if it was not detected correctly. |
81d5b81fb3c2
Added help for all tools in the toolkit. Many bug fixes and a few new nucleosome tools.
timpalpant
parents:
4
diff
changeset
|
32 |
81d5b81fb3c2
Added help for all tools in the toolkit. Many bug fixes and a few new nucleosome tools.
timpalpant
parents:
4
diff
changeset
|
33 ----- |
4 | 34 |
12
81d5b81fb3c2
Added help for all tools in the toolkit. Many bug fixes and a few new nucleosome tools.
timpalpant
parents:
4
diff
changeset
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35 **Example** |
81d5b81fb3c2
Added help for all tools in the toolkit. Many bug fixes and a few new nucleosome tools.
timpalpant
parents:
4
diff
changeset
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36 |
81d5b81fb3c2
Added help for all tools in the toolkit. Many bug fixes and a few new nucleosome tools.
timpalpant
parents:
4
diff
changeset
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37 Calculate the mean change in nucleosome occupancy for each gene in the yeast genome: |
81d5b81fb3c2
Added help for all tools in the toolkit. Many bug fixes and a few new nucleosome tools.
timpalpant
parents:
4
diff
changeset
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38 |
81d5b81fb3c2
Added help for all tools in the toolkit. Many bug fixes and a few new nucleosome tools.
timpalpant
parents:
4
diff
changeset
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39 - 1. Create a "change in occupancy" dataset by subtracting the normalized occupancy Wig files from your two conditions using the WigMath -> Subtract tool. |
81d5b81fb3c2
Added help for all tools in the toolkit. Many bug fixes and a few new nucleosome tools.
timpalpant
parents:
4
diff
changeset
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40 - 2. Upload a list of intervals corresponding to the genes in the yeast genome, or pull the data from UCSC using Get Data -> UCSC Main. |
81d5b81fb3c2
Added help for all tools in the toolkit. Many bug fixes and a few new nucleosome tools.
timpalpant
parents:
4
diff
changeset
|
41 - 3. Calculate the mean change in occupancy for each gene using this tool and the datasets from (1) and (2). |
81d5b81fb3c2
Added help for all tools in the toolkit. Many bug fixes and a few new nucleosome tools.
timpalpant
parents:
4
diff
changeset
|
42 |
4 | 43 </help> |
44 </tool> |