view galaxy-conf/ZScore.xml @ 15:3e477c7e0e73 draft

Update normalization tools to allow normalizing by chromosome (Z-scorer, scale, etc.) Add new tool to convert any interval file to Bed format. Miscellaneous bug fixes.
author timpalpant
date Sat, 09 Jun 2012 16:03:59 -0400
parents eb53be9a09f4
children
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<tool id="WigZScore" name="Z-Score" version="1.2.0">
  <description>a (Big)Wig file</description>
  <command interpreter="sh">galaxyToolRunner.sh wigmath.ZScore -i $input $chr -o $output</command>
  <inputs>
      <param format="bigwig,wig" name="input" type="data" label="Z-score the data in" />
      <param name="chr" type="boolean" checked="false" truevalue="-c" falsevalue="" label="Z-score chromosomes individually" />
  </inputs>
  <outputs>
      <data format="wig" name="output" metadata_source="input" />
  </outputs>
  <tests>
	  <test>
	    <param name="input" value="wigmath1.wig"/>
	    <output name="output" file="zscorer1.wig"/>
	  </test>
	  <test>
	    <param name="input" value="wigmath2.wig"/>
	    <output name="output" file="zscorer2.wig"/>
	  </test>
	  <test>
	    <param name="input" value="wigmath3.wig"/>
	    <output name="output" file="zscorer3.wig"/>
	  </test>
	  <test>
	    <param name="input" value="wigmath1.bw"/>
	    <output name="output" file="zscorer4.wig"/>
	  </test>
	  <test>
	    <param name="input" value="wigmath2.bw"/>
	    <output name="output" file="zscorer5.wig"/>
	  </test>
	  <test>
	    <param name="input" value="wigmath3.bw"/>
	    <output name="output" file="zscorer6.wig"/>
	  </test>
  </tests>
  
  <help>

This tool will compute normal scores (Z-scores) for each of the values in a Wig file. For each base pair, the Z-scored value is equal to the deviance from the mean divided by the standard deviation (i.e. the number of standard deviations a value is away from the mean). The output file should have mean 0 and standard deviation 1.

.. class:: infomark

This tool is equivalent to using the **Mean Shift** tool to shift a Wig file to mean 0, then using the **Scale** tool to scale by 1/(standard deviation).

.. class:: infomark

**TIP:** If your dataset does not appear in the pulldown menu, it means that it is not in Wig or BigWig format. Use "edit attributes" to set the correct format if it was not detected correctly.

  </help>
</tool>