view galaxy-conf/IntervalAverager.xml @ 13:eb53be9a09f4 draft

Uploaded
author timpalpant
date Sat, 19 May 2012 10:36:45 -0400
parents 81d5b81fb3c2
children 3e477c7e0e73
line wrap: on
line source

<tool id="IntervalAverager" name="Average intervals" version="1.0.0">
  <description>that have been aligned</description>
  <command interpreter="sh">
    galaxyToolRunner.sh visualization.IntervalAverager -l $loci -o $output $file1
      #for $input in $inputs
        ${input.file}
      #end for
  </command>
  <inputs>
      <param name="file1" label="Sequencing data" type="data" format="bigwig,wig" />
      <repeat name="inputs" title="Additional sequencing data">
        <param name="file" type="data" format="bigwig,wig" />
      </repeat>
      <param format="bed" name="loci" type="data" label="List of intervals (with alignment points)" />
  </inputs>
  <outputs>
      <data format="tabular" name="output" />
  </outputs>
  
<help>

This tool calculates the average signal for a set of aligned intervals. Intervals are lined up on their alignment point (column 5 in the Bed file), flipped if on the - strand, and averaged. The output is equivalent to aligning the data in a matrix and then taking the columnwise average of the matrix.

Intervals with alignment points must be provided in the following extended Bed format ::

  chr  low  high  id  alignment  strand

.. class:: infomark

**TIP:** If your dataset does not appear in the pulldown menu, it means that it is not in Wig or BigWig format. Use "edit attributes" to set the correct format if it was not detected correctly.

-----

**Syntax**

- **Sequencing data** is the genomic data used to create the average
- **List of intervals** is a list of intervals in Bed format with alignment points

</help>
</tool>