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annotate orthologs/ucsb_hamster/README_UCSBhamster @ 0:5b9a38ec4a39 draft default tip
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author | osiris_phylogenetics <ucsb_phylogenetics@lifesci.ucsb.edu> |
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date | Tue, 11 Mar 2014 12:19:13 -0700 |
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1 Dependancies that must be installed and available in path: |
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2 blastall |
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3 formatdb |
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4 |
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5 Dependancies distributed with hamster and galaxy hamster |
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6 BioPerl |
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7 genewise |
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8 rungenewise.pm |
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9 wisecfg -- directory of configuration files for genewise |
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10 |
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11 |
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12 |
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13 QUICKSTART |
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14 |
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15 1. Copy galaxy_hamster files into the directory where you want to |
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16 install the program. |
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17 |
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18 2. execute the install.pl script to configure required directories. |
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19 Type: |
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20 |
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21 ./install.pl |
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22 |
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23 3. Make sure blastall and formatdb are installed on your system, |
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24 and have been configured on your PATH. |
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25 |
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26 4. add ucsb_hamster.xml to Galaxy's tool_conf.xml file. |
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27 |
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28 5. Restart Galaxy |
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29 |
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30 |
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31 Now, ucsb hamster can be used using HMM's constructed in a Galaxy |
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32 history using phytab_hmmbuild. |
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33 |
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34 |
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35 |
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36 |
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37 |
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38 |
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39 DETAILED INSTALLATION PROCEDURES: |
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40 |
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41 If for some reason install.pl does not work, first make sure that |
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42 you have deleted the PATH variable pointing to any existing galaxy_hamster |
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43 installation on your system in the .bashrc file. |
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44 |
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45 If you have deleted this variable and still cannot execute the install script, |
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46 you may follow the detailed installation procedure below. |
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47 |
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48 1. Copy galaxy_hamster files into the directory where you want to install |
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49 the program. |
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50 |
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51 2. Directory that contains the following scripts must be in the overall |
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52 path: |
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53 translate.pl |
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54 emap2fasta.pl |
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55 unbuild.py |
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56 |
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57 You may add the directory into your .bashrc file located in your HOME directory. |
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58 |
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59 For example: |
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60 export PATH="/home/galaxy/galaxy-dist/tools/galaxy_hamster:$PATH"; |
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61 |
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62 3. Type: source .bashrc to reload your environment variables. |
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63 |
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64 4. In the file run_genewise.pm located in the lib direcotry, you must set the full |
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65 path of the genewise configuration directory. The configurat iondirectory is |
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66 called wisecfg and it is distributed with galaxy_hamster. |
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67 |
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68 5. From here, ensure that blastall and formatdb are installed on your system and |
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69 have been configured on your PATH. |
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70 |
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71 6. Add ucsb_hamster.xml to Galaxy's tool_conf.xml file. |
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72 |
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73 7. When installing on an Ubuntu system, the ucsb_hamster.sh script was not finding |
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74 translate.pl even though it was in the path. To fix this I (THO) added translate.pl and |
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75 the main hamster script to /usr/local/bin/ with a symbolic link, which seemed to fix |
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76 this problem. |
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77 |
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78 8. Restart Galaxy. |