Mercurial > repos > vandelj > giant_aptsummarize
annotate galaxy/wrappers/ArrayNormalization.xml @ 0:708f43bda2b6 draft
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
author | vandelj |
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date | Fri, 26 Jun 2020 09:35:11 -0400 |
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children | 2762a2622e0d |
rev | line source |
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0
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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1 <tool name="GIANT-Normalization with APT Summarize" id="giant_aptsummarize" version="0.1.1"> |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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2 <description>Apply Affymetrix Power Tool summarize function to .CEL collection</description> |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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3 <requirements> |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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4 <requirement type="package" version="2.10.1">apt-probeset-summarize</requirement> |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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5 </requirements> |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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6 <stdio> |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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7 <regex match="Execution halted" |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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8 source="both" |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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9 level="fatal" |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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10 description="Execution halted, please contact tool developer or administrators." /> |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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11 <exit_code range="10" level="fatal" description="Error in post-treatments, see log file for more information." /> |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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12 <exit_code range="1:9" level="fatal" description="Error during apt command execution, see log file for more information." /> |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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13 </stdio> |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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14 |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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15 |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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16 |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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17 <command> <![CDATA[ |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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18 ##ONLY FOR LOCAL TEST PURPOSE, COMMENT OTHERWISE |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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19 ##set $pathToApt='/mnt/galaxy/home/galaxy/Software/ThermoFischer/apt-1.20.6-x86_64-intel-linux/bin' |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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20 |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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21 mkdir ./apt_output/; |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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22 |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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23 #if $mainCondition.arraySelection=="other": |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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24 #if $CDFfile: |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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25 ##.cdf file should has original name |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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26 cp $CDFfile ./${CDFfile.name}; |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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27 #end if |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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28 #end if |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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29 |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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30 |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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31 ##run APT command |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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32 |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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33 ##ONLY FOR LOCAL TEST PURPOSE COMMENT IF NOT NECESSARY |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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34 ##${pathToApt}/apt-probeset-summarize -a $commandLine |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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35 ##AND UNCOMMENT LINE BELOW |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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36 apt-probeset-summarize -a $commandLine |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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37 |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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38 #if $mainCondition.arraySelection=="other": |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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39 #if $PGFfile and $CLFfile: |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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40 --use-pgf-names |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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41 -p $PGFfile |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
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42 -c $CLFfile |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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43 #end if |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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44 #if $CDFfile: |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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45 -d ./${CDFfile.name} |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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46 #end if |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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47 #if $MPSfile and $normLevel=="core": |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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48 -m $MPSfile |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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49 #end if |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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50 #if $BGPfile: |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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51 -b $BGPfile |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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52 #end if |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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53 |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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54 #else: |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
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55 |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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56 #if $mainCondition.arrayID.fields.pathPGF!="null" and $mainCondition.arrayID.fields.pathCLF!="null": |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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57 --use-pgf-names |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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58 -p $mainCondition.arrayID.fields.pathPGF |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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59 -c $mainCondition.arrayID.fields.pathCLF |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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60 #else: |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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61 #if $mainCondition.arrayID.fields.pathCDF!="null": |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
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62 -d $mainCondition.arrayID.fields.pathCDF |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
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63 #end if |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
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64 #end if |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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65 #if $normLevel=="core" and $mainCondition.arrayID.fields.pathMPS!="null": |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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66 -m $mainCondition.arrayID.fields.pathMPS |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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67 #end if |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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68 #if $mainCondition.arrayID.fields.pathBGP!="null": |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
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69 -b $mainCondition.arrayID.fields.pathBGP |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
parents:
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70 #end if |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
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71 #end if |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
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72 |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
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73 -o ./apt_output/ |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
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74 |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
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75 \$(echo '$inputData' | tr "," " ") >> $log 2>&1; |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
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76 |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
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77 ret_code=\$?; |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
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78 |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
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79 printf "[INFO]Normalized files: "$inputData >> $log; |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
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80 |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
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81 if [ \$ret_code != 0 ]; then |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
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82 exit \$ret_code; |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
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83 else |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
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84 |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
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85 ##launch post-treatment scripts |
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86 |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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87 bash $scriptFormat; |
708f43bda2b6
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88 #if $annotationCondition.addAnnotation=="true": |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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89 echo "[INFO]Annotation file used:" >> $log; |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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90 #if $mainCondition.arraySelection=="other": |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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91 bash $scriptAnnotation $mainCondition.annotationFile; |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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92 echo $mainCondition.annotationFile.name >> $log; |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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93 #else: |
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94 #if $normLevel=="core": |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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95 bash $scriptAnnotation $mainCondition.arrayID.fields.pathAnnotTrans; |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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96 #else: |
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97 bash $scriptAnnotation $mainCondition.arrayID.fields.pathAnnotProbe; |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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98 #end if |
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99 cat $mainCondition.arrayID.fields.versionInfo >> $log; |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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100 #end if |
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101 #end if |
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102 fi; |
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103 printf "[INFO]End of tool script" >> $log; |
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"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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104 ]]> |
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105 </command> |
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106 |
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107 |
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108 |
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109 <configfiles> |
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110 <configfile name="scriptFormat"> |
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111 <![CDATA[ |
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112 #for $inputDataset in $inputData |
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113 echo '${inputDataset.name}' >> ./tempColumnName.txt |
708f43bda2b6
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114 #end for |
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115 awk ' BEGIN{firstLine=1;OFS="";ORS=""} ARGIND==1{tab[FNR]=$1;nbCol=FNR} ARGIND==2 && !($1 ~ /\\#/){if(firstLine==0){print "\n"\$0}else{print "Conditions";for(i=1;i<=nbCol;i++){print "\t"tab[i]};firstLine=0}}' ./tempColumnName.txt ./apt_output/*summary.txt > $outputData |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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116 |
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117 if [ ! -e $outputData ]; then |
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118 printf "[ERROR]Formating results failed" >> $log; |
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119 exit 10 |
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120 fi |
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121 ]]> |
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122 </configfile> |
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123 |
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124 <configfile name="scriptAnnotation"> |
708f43bda2b6
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125 <![CDATA[ |
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126 #if $annotationCondition.addAnnotation=="true": |
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"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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127 #if $annotationCondition.mergingMethod=="none": |
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128 |
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129 #if $annotationCondition.keepAnnotated=="true": |
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130 awk 'BEGIN{OFS="\t"} ARGIND==1{dico[\$1]=\$2} ARGIND==2 && FNR==1{print \$0} ARGIND==2 && FNR>1{if(\$1 in dico){\$1=dico[\$1]"_("\$1")";print \$0}}' \$1 $outputData > ./tempData |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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131 #else: |
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132 awk 'BEGIN{OFS="\t"} ARGIND==1{dico[\$1]=\$2} ARGIND==2 && FNR==1{print \$0} ARGIND==2 && FNR>1{if(\$1 in dico){\$1=dico[\$1]"_("\$1")"};print \$0}' \$1 $outputData > ./tempData |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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133 #end if |
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134 |
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135 #end if |
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136 #if $annotationCondition.mergingMethod=="mean": |
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"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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137 |
708f43bda2b6
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138 #if $annotationCondition.keepAnnotated=="true": |
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"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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139 awk 'BEGIN{OFS="\t"} ARGIND==1{dico[\$1]=\$2} ARGIND==2 && FNR==1{print \$0;nbCol=NF} ARGIND==2 && FNR>1{if(\$1 in dico){cpt[dico[\$1]]++;for(i=2;i<=nbCol;i++)sum[dico[\$1]][i]+=\$i}} END{for(iName in cpt){line=iName;for(iCol=2;iCol<=nbCol;iCol++){line=line"\t"sum[iName][iCol]/cpt[iName]};print line}}' \$1 $outputData > ./tempData |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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140 #else: |
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141 awk 'BEGIN{OFS="\t"} ARGIND==1{dico[\$1]=\$2} ARGIND==2 && FNR==1{print \$0;nbCol=NF} ARGIND==2 && FNR>1{if(\$1 in dico){cpt[dico[\$1]]++;for(i=2;i<=nbCol;i++)sum[dico[\$1]][i]+=\$i}else{print \$0}} END{for(iName in cpt){line=iName;for(iCol=2;iCol<=nbCol;iCol++){line=line"\t"sum[iName][iCol]/cpt[iName]};print line}}' \$1 $outputData > ./tempData |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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142 #end if |
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143 |
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144 #end if |
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145 |
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146 #if $annotationCondition.mergingMethod=="higherVar": |
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"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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147 |
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148 #if $annotationCondition.keepAnnotated=="true": |
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"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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149 awk 'BEGIN{OFS="\t"} ARGIND==1{dico[\$1]=\$2} ARGIND==2 && FNR==1{print \$0;nbCol=NF} ARGIND==2 && FNR>1{if(\$1 in dico){mean=0;for(i=2;i<=nbCol;i++){mean+=\$i};mean=mean/(nbCol-1);curVar=0;for(i=2;i<=nbCol;i++){curVar+=(mean-\$i)^2};if(curVar>var[dico[\$1]]){var[dico[\$1]]=curVar;for(i=2;i<=nbCol;i++){bestVar[dico[\$1]][i]=\$i}}}} END{for(iName in bestVar){line=iName;for(iCol=2;iCol<=nbCol;iCol++){line=line"\t"bestVar[iName][iCol]};print line}}' \$1 $outputData > ./tempData |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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150 #else: |
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151 awk 'BEGIN{OFS="\t"} ARGIND==1{dico[\$1]=\$2} ARGIND==2 && FNR==1{print \$0;nbCol=NF} ARGIND==2 && FNR>1{if(\$1 in dico){mean=0;for(i=2;i<=nbCol;i++){mean+=\$i};mean=mean/(nbCol-1);curVar=0;for(i=2;i<=nbCol;i++){curVar+=(mean-\$i)^2};if(curVar>var[dico[\$1]]){var[dico[\$1]]=curVar;for(i=2;i<=nbCol;i++){bestVar[dico[\$1]][i]=\$i}}}else{print \$0}} END{for(iName in bestVar){line=iName;for(iCol=2;iCol<=nbCol;iCol++){line=line"\t"bestVar[iName][iCol]};print line}}' \$1 $outputData > ./tempData |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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152 #end if |
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153 |
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154 #end if |
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155 |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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156 #if $annotationCondition.mergingMethod=="lowerVar": |
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"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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157 |
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158 #if $annotationCondition.keepAnnotated=="true": |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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159 awk 'BEGIN{OFS="\t"} ARGIND==1{dico[\$1]=\$2} ARGIND==2 && FNR==1{print \$0;nbCol=NF} ARGIND==2 && FNR>1{if(\$1 in dico){mean=0;for(i=2;i<=nbCol;i++){mean+=\$i};mean=mean/(nbCol-1);curVar=0;for(i=2;i<=nbCol;i++){curVar+=(mean-\$i)^2};if(var[dico[\$1]]==0 || curVar<var[dico[\$1]]){var[dico[\$1]]=curVar;for(i=2;i<=nbCol;i++){bestVar[dico[\$1]][i]=\$i}}}} END{for(iName in bestVar){line=iName;for(iCol=2;iCol<=nbCol;iCol++){line=line"\t"bestVar[iName][iCol]};print line}}' \$1 $outputData > ./tempData |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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160 #else: |
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161 awk 'BEGIN{OFS="\t"} ARGIND==1{dico[\$1]=\$2} ARGIND==2 && FNR==1{print \$0;nbCol=NF} ARGIND==2 && FNR>1{if(\$1 in dico){mean=0;for(i=2;i<=nbCol;i++){mean+=\$i};mean=mean/(nbCol-1);curVar=0;for(i=2;i<=nbCol;i++){curVar+=(mean-\$i)^2};if(var[dico[\$1]]==0 || curVar<var[dico[\$1]]){var[dico[\$1]]=curVar;for(i=2;i<=nbCol;i++){bestVar[dico[\$1]][i]=\$i}}}else{print \$0}} END{for(iName in bestVar){line=iName;for(iCol=2;iCol<=nbCol;iCol++){line=line"\t"bestVar[iName][iCol]};print line}}' \$1 $outputData > ./tempData |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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162 #end if |
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163 |
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164 #end if |
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165 |
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166 if [ -e ./tempData ]; then |
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167 mv -f ./tempData $outputData |
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168 else |
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169 printf "[ERROR]Annotation merge failed" >> $log; |
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170 exit 10 |
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171 fi |
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172 |
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173 #end if |
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174 |
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175 ]]> |
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176 </configfile> |
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177 </configfiles> |
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178 |
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179 |
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180 |
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181 <inputs> |
708f43bda2b6
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182 <param type="text" name="title" value="APT_toPersonalize" label="Title for output"/> |
708f43bda2b6
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183 |
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184 <param type="data" name="inputData" format="cel" label=".cel collection file" optional="false" multiple="true"> |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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185 <validator type="empty_dataset" message="At least one data file should be selected"></validator> |
708f43bda2b6
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186 </param> |
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187 |
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188 <param type="select" name="commandLine" display="radio" label="Normalization to perform" help="For more details go to APT webpage"> |
708f43bda2b6
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189 <option value="rma-gc-scale" selected="true">gc correction + scale intensity + rma</option> |
708f43bda2b6
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190 <option value="scale-intensities,rma-bg,quant-norm.sketch=0.usepm=true.bioc=true,pm-only,med-polish">scale intensity + rma</option> |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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191 <option value="gc-correction,rma-bg,quant-norm.sketch=0.usepm=true.bioc=true,pm-only,med-polish">gc correction + rma</option> |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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192 <option value="rma">rma</option> |
708f43bda2b6
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193 </param> |
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194 |
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195 <param type="select" name="normLevel" display="radio" label="Normalization level" help="'Core genes' option is not available for all arrays"> |
708f43bda2b6
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196 <option value="core">Core genes</option> |
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197 <option value="probeset">Probe set</option> |
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198 </param> |
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199 |
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200 <conditional name="mainCondition"> |
708f43bda2b6
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201 |
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202 <param name="arraySelection" type="select" label="Select GeneChip array configuration files"> |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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203 <option value="common">pre-loaded array files</option> |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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204 <option value="other">array files selection</option> |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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205 </param> |
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206 |
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207 <when value="common"> |
708f43bda2b6
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208 <param name="arrayID" label="Name" type="select"> |
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"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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209 <options from_data_table="aptTool"> |
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210 </options> |
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211 </param> |
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212 </when> |
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213 |
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214 <when value="other"> |
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215 |
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216 <param type="data" format="data" name="PGFfile" label=".pgf indicates which probes are grouped together into a probeset*" optional="true" multiple="false"> |
708f43bda2b6
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217 </param> |
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218 |
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219 <param type="data" format="data" name="CLFfile" label=".clf indicates where the probes are located in the CEL file*" optional="true" multiple="false"> |
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220 </param> |
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221 |
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222 <param type="data" format="data" name="CDFfile" label=".cdf contains both .pgf and .clf informations* (requested only if .pgf and .clf files are not available)" optional="true" multiple="false"> |
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"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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223 </param> |
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224 |
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225 <param type="data" format="data" name="MPSfile" label=".mps defines a probeset as a collection of existing probesets* (requested for normalization at gene level)" optional="true" multiple="false" help="If .mps is not selected/available a 'probeset level' normalization will be automatically performed"> |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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226 </param> |
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227 |
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228 <param type="data" format="data" name="BGPfile" label=".bgp indicates which probes are to be used for computing background* (requested only if GC driven background correction is applied)" optional="true" multiple="false" help="*Files should be downloaded from corresponding array specific Affymetrix webpage to insure format compatibility"> |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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229 </param> |
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230 |
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231 <param type="data" name="annotationFile" format="tabular" label="tabular file containing available probesets annotation (requested if annotation options are selected)" optional="true" multiple="false" > |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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232 </param> |
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233 |
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234 </when> |
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235 </conditional> |
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236 |
708f43bda2b6
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237 <conditional name="annotationCondition"> |
708f43bda2b6
"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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238 <param type="boolean" name="addAnnotation" checked="false" label="Add gene annotation"> |
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239 </param> |
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240 <when value="true"> |
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241 <param type="boolean" name="keepAnnotated" checked="false" label="Discard probe set without gene annotation"> |
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242 </param> |
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243 |
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244 <param type="select" name="mergingMethod" display="radio" label="Merging approach for probe set with same gene annotation"> |
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245 <option value="none">No merging</option> |
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246 <option value="mean" selected="true">Mean between probes [recommended]</option> |
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247 <option value="higherVar">Keep probe with higher variance</option> |
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248 <option value="lowerVar">Keep probe with lower variance</option> |
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249 </param> |
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250 </when> |
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251 <when value="false"> |
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252 </when> |
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253 </conditional> |
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254 </inputs> |
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255 |
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256 |
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257 |
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258 <outputs> |
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259 <data format="tabular" name="outputData" label="${title}_NormalizedData"/> |
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260 <data format="txt" name="log" label="${title}_Log" /> |
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261 </outputs> |
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262 |
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263 |
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264 |
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265 <tests> |
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266 <test maxseconds="3600"> |
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267 <param name="inputData" value="./CELfiles/GSM205766.CEL,./CELfiles/GSM205767.CEL,./CELfiles/GSM205768.CEL" /> |
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268 <param name="commandLine" value="rma-gc-scale" /> |
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269 <param name="normLevel" value="core" /> |
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270 <param name="arraySelection" value="other" /> |
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271 <param name="CDFfile" value="./Mouse430_2.cdf" /> |
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272 <param name="annotationFile" value="./formatedAnnotation.csv" /> |
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273 <param name="addAnnotation" value="true" /> |
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274 <param name="keepAnnotated" value="false" /> |
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275 <param name="mergingMethod" value="mean" /> |
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276 <output name="outputData" file="./APT-summarize/expressionOutput.csv" /> |
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277 <output name="log" file="./APT-summarize/output.log" lines_diff="2" /> |
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278 </test> |
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279 </tests> |
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280 |
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281 |
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282 |
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283 |
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284 <help> |
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285 <![CDATA[ |
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286 **What it does** |
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287 |
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288 To normalize expression data from Affymetrix GeneChip arrays through Affymetrix Power Tools software. |
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289 |
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290 ----- |
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291 |
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292 **Parameters** |
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293 |
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294 \- **Title** to personalize output file names (please avoid special characters). |
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295 |
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296 |
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297 \- **.CEL files** you want to normalize (you can select multiple .CEL files or unique collection). |
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298 |
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299 |
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300 \- **Analysis to perform** : as this tool run the 'apt-probeset-summarize' command line from Affymetrix Power Tool software, this field represents the desired analysis pathway (-a parameter). Analysis pathways are summarized through predifined pathway alias with full description of all run steps below: |
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301 |
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302 - gc correction + scale intensity + rma: "gc-correction,scale-intensities,rma-bg,quant-norm.sketch=0.usepm=true.bioc=true,pm-only,med-polish" |
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303 |
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304 - scale intensity + rma: "scale-intensities,rma-bg,quant-norm.sketch=0.usepm=true.bioc=true,pm-only,med-polish" |
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305 |
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306 - gc correction + rma: "gc-correction,rma-bg,quant-norm.sketch=0.usepm=true.bioc=true,pm-only,med-polish" |
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307 |
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308 - rma: "rma-bg,quant-norm.sketch=0.usepm=true.bioc=true,pm-only,med-polish" |
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309 |
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310 Please refer to `APT tool web page`__ to get more details about analysis steps. |
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311 |
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312 \- **Normalization level** to choose when GeneChip array kind allows such distinction (ie. when a .mps file exists in Affymetrix webpage). 'Probe set level' is the first level where probes for same exon are merged in probe sets. 'Gene level' [recommended] is an upper level where several probe sets are merged in meta probe sets. If a meta probe set can be see as a good single gene expression indicator, several meta probe sets can still share same gene annotation. |
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313 |
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314 \- **GeneChip array configuration files** according to .CEL files. Configuration files for given arrays can be pre-loaded by administrators of the used Galaxy instance and listed in the aptTool.loc file. In this case you just have to select the array kind according to .CEL files, thus pre-loaded configuration files will be used automatically for normalization. If configuration files were not pre-loaded, you have to choose "array files selection" and then select manually configuration files for the corresponding array. Configuration files (.pgf, .clf, .cdf, .mps, .bgp) are available in library archive files from the Affymetrix webpage of the array (as an example, configuration files for HTA2.0 array are found in the 'Main Analysis Files' in the 'Support file' section of `this page`__). All these files are not mandatory for all normalizations. For basic normalization, both .pgf and.clf files or only .cdf file are mandatory. .mps file is requested for gene level normalization if it is available (see Normalization level parameter). .bgp file is requested for analysis pathway with specific background computation. Annotation file is a simple two column tabular file, with first column containing probe set names and second corresponding annotations, it should not contains any column header. This file can be generated from Affymetrix annotation file available in the same section as array configuration files in Affymetrix webpages, please keep a trace of used annotation version to insure reproducibility. |
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315 |
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316 Extract of annotation file:: |
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317 |
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318 TC01000020.hg.1 SAMD11 |
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319 TC01000021.hg.1 KLHL17 |
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320 TC01000022.hg.1 PLEKHN1 |
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321 TC01000023.hg.1 ISG15 |
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322 TC01000024.hg.1 AGRN |
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323 |
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324 \- **Gene annotation** : add gene annotation when available (for pre-loaded arrays, annotation file path should be filled in the aptTool.loc file, for other arrays, annotation file should be selected manually). You can choose to keep or discard in normalized file (meta) probe sets without match in annotation file and select merging policy for probe sets sharing same gene annotation. |
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325 |
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326 ----- |
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327 |
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328 **Advanced parameters** |
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329 |
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330 \- **Additional parameter** to give to 'apt-probeset-summarize' command line except -a, -o, -d, -p, -c, -m, -b parameters already filled by previous fields. |
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331 |
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332 ----- |
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333 |
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334 **Outputs** |
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335 |
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336 \- **tabular file** containing log2 transformed normalized data as tab delimited matrix. First row contains .CEL names and first column contains (meta) probe set names and/or annotated gene names:: |
708f43bda2b6
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337 |
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338 Conditions 157_(HuGene-2_0-st).CEL 156_(HuGene-2_0-st).CEL 155_(HuGene-2_0-st).CEL 154_(HuGene-2_0-st).CEL |
708f43bda2b6
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339 DDX11L2 4.500872 4.429759 4.780281 4.996189 |
708f43bda2b6
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340 MIR1302-2 3.415065 3.520472 3.471503 3.567988 |
708f43bda2b6
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341 OR4F5 3.737956 3.011586 3.424494 3.497545 |
708f43bda2b6
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342 VWA1 5.189621 5.129595 4.806793 5.227014 |
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343 |
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344 \- **LOG file** containing information about execution. Useful especially if tool execution fais. Please attach this log file in any bug report. |
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345 |
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346 .. __: http://media.affymetrix.com/support/developer/powertools/changelog/apt-probeset-summarize.html#intro |
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347 |
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348 .. __: https://www.thermofisher.com/order/catalog/product/902233#/902233 |
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349 |
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350 ]]> |
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351 </help> |
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352 |
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353 |
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354 <citations> |
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355 <citation type="bibtex">@misc{vandel_jimmy_2018_1477870, author = {Vandel, J. and Gheeraert, C. and Eeckhoute, J. and Staels, B. and Lefebvre, P. and Dubois-Chevalier, J.}, title = {GIANT: Galaxy-based Interactive tools for ANalaysis of Transcriptomic data}, month = nov, year = 2018, doi = {10.5281/zenodo.1477870}, url = {https://doi.org/10.5281/zenodo.1477870} |
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356 }</citation> |
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357 |
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358 <citation type="bibtex">@online{apt, author = {Thermo Fisher Scientific}, title = {Affymetrix Power Tools}, publisher = {Life Technologies Corp.}, address = {USA, MA}, version = {2.10.2}, year = {2018}, url = {https://www.affymetrix.com/support/developer/powertools/changelog/index.html} |
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359 }</citation> |
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360 </citations> |
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361 </tool> |