Mercurial > repos > vandelj > giant_gsea_format

annotate galaxy/wrappers/FormatForGSEA.xml @ 0:3022feec50fe draft

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"planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
author vandelj
date Fri, 26 Jun 2020 09:36:46 -0400
parents
children d72f1bc5ce9e
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3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
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1 <tool name="GIANT-GSEA Formatting" id="giant_gsea_format" version="0.2.0">
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
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2 <description>Format input files for GSEA software</description>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
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3 <code file="../../src/General_functions.py"/>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
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4 <stdio>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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5 <regex match="Execution halted"
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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6 source="both"
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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7 level="fatal"
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8 description="Execution halted." />
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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9 <exit_code range="10" level="fatal" description="Error during file generation, see log file for more information." />
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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10 </stdio>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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11 <command> <![CDATA[
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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12 #if $mainCondition.selection=="classicGSEA":
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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13
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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14 awk 'BEGIN{FS="\t";OFS="";ORS="";nlines=0} ARGIND==1 && FNR>1{nlines++} ARGIND==2 && FNR==1{print "\#1.2\n"nlines"\t"NF-1"\n";print "NAME\tDESCRIPTION"; for(i=2;i<=NF;i++)print"\t"\$i;print "\n"} ARGIND==2 && FNR>1{print \$1"\tna";for(i=2;i<=NF;i++)print "\t"\$i;print "\n"}' $mainCondition.expressionData $mainCondition.expressionData > $outExpression;
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
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16 if [ ! -e $outExpression ]; then
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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17 printf "[ERROR]Expression file failed" >> $log;
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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18 exit 10;
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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19 fi
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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20 ;
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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21 awk -v factor="$mainCondition.factorToInclude" 'BEGIN{FS="\t";OFS="";ORS="";nameCond="";line="";cpt=0;lgt=0} ARGIND==1 && FNR==1{for(iCond=2;iCond<=NF;iCond++){conditionOrder[iCond-1]=\$iCond};cpt=NF-1} ARGIND==2 && FNR==1{for(i=1;i<=NF;i++)if(\$i==factor)factorInd=i} ARGIND==2 && FNR>1{valueFact[\$1]=\$factorInd} END{for(i=1;i<=cpt;i++){ line=line""valueFact[conditionOrder[i]]" "; if(dico[valueFact[conditionOrder[i]]]!=1){lgt++; nameCond=nameCond""valueFact[conditionOrder[i]]" ";dico[valueFact[conditionOrder[i]]]=1}};print cpt" "lgt" 1\n";print "\# "nameCond"\n";print line}' $mainCondition.expressionData $mainCondition.conditionInformation > $outPhenotypes;
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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22
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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23 if [ ! -e $outPhenotypes ]; then
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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24 printf "[ERROR]Phenotype file failed" >> $log;
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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25 exit 10;
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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26 fi
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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27 ;
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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28 #else:
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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29
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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30 if [ \$(awk 'NR==1{print (NF-2)%5}' $mainCondition.differentialAnalysis ) -ne 0 ]; then
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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31 printf "[ERROR] please check that differential analysis file respect requested input format especially concerning the column number" >> $log;
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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32 exit 10;
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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33 fi
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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34 ;
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35
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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36 awk -v comparison="$mainCondition.comparisonsToUse" -v rkIndice="$mainCondition.rankingIndice" -v pvalTh="$mainCondition.pvalThreshold" 'BEGIN{FS="\t"} NR==1{if(rkIndice=="Tstat" || rkIndice=="absTstat"){start=7}else{start=6};for(i=start;i<=NF;i=i+5){if(\$i==comparison)refCol=i};} NR>2{if(rkIndice=="Tstat" || rkIndice=="absTstat"){if(\$(refCol-3)<pvalTh){if(rkIndice=="Tstat"){val=\$refCol}else{if(\$refCol>0){val=\$refCol}else{val=-\$refCol}};print \$1"\t"val}}else{if(\$(refCol-2)<pvalTh){if(rkIndice=="FC"){val=\$refCol}else{if(\$refCol>0){val=\$refCol}else{val=-\$refCol}};print \$1"\t"val}}}' $mainCondition.differentialAnalysis > ./temp.txt;
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
vandelj
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37 printf "#NAME\tSCORE\n" > $outRankedGenes;
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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38 LC_ALL=C sort -t$'\t' -k2,2 -gr ./temp.txt >> $outRankedGenes;
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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39 rm ./temp.txt;
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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40
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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41 if [ ! -e $outRankedGenes ]; then
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42 printf "[ERROR]Rank file failed" >> $log;
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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43 exit 10;
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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44 fi
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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45 ;
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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46 #end if
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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47
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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48 printf "[INFO]End of tool script" >> $log;
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49 ]]>
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50 </command>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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51 <inputs>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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52 <param type="text" name="title" value="GSEAformat_toPersonalize" label="Title for output (without space)"/>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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53 <conditional name="mainCondition">
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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54 <param name="selection" type="select" label="GSEA configuration" force_select="true">
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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55 <option value="classicGSEA">GSEA analysis </option>
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56 <option value="rankedGSEA">Pre-ranked GSEA analysis</option>
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57 </param>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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58 <when value="classicGSEA">
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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59 <param type="data" name="expressionData" format="tabular" label="Normalized expression tabular file" optional="false" multiple="false">
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60 </param>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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61 <param type="data" name="conditionInformation" format="tabular" label="Factor information tabular file" optional="false" multiple="false">
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62 </param>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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63 <param name="factorToInclude" type="select" label="Reference factor" multiple="false" optional="false" refresh_on_change="true"
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64 dynamic_options="get_column_names_filteredList(mainCondition['conditionInformation'].file_name,[0])">
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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65 </param>
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66 </when>
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67 <when value="rankedGSEA">
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68 <param type="data" name="differentialAnalysis" format="tabular" label="Differential analysis tabular file (as given by LIMMA diff.exp. tool)" multiple="false" help="This file should contain only annotated gene names or only probe identifiers as rows but no both kinds.">
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69 </param>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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70 <param name="comparisonsToUse" type="select" label="Reference contrast" optional="false" multiple="false" refresh_on_change="true" dynamic_options="get_column_names_filteredList(mainCondition['differentialAnalysis'].file_name,[0,1],5)">
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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71 <validator type="empty_field" message="You should specify one factor"></validator>
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72 </param>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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73 <param type="select" name="rankingIndice" display="radio" label="Reference statistic">
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74 <option value="FC">Relative value of Log2(Fold Change)</option>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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75 <option value="absFC">Absolute value of Log2(Fold Change)</option>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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76 <option value="Tstat">Relative value of moderated t-statistic</option>
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77 <option value="absTstat">Absolute value of moderated t-statistic</option>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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78 </param>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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79 <param name="pvalThreshold" type="float" value="0.05" label="FDR p-val threshold">
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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80 <validator type="in_range" min="0" max="1" exclude_min="true" message="Threshold should be between 0 and 1"/>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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81 </param>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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82 </when>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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83 </conditional>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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84 </inputs>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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85
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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86 <outputs>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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87 <data format="gct" name="outExpression" label="${title}_Expressions">
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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88 <filter>mainCondition['selection']=="classicGSEA"</filter>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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89 </data>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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90
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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91 <data format="cls" name="outPhenotypes" label="${title}_Phenotypes">
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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92 <filter>mainCondition['selection']=="classicGSEA"</filter>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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93 </data>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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94
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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95 <data format="rnk" name="outRankedGenes" label="${title}_Ranked_Genes">
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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96 <filter>mainCondition['selection']=="rankedGSEA"</filter>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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97 </data>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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98
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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99 <data format="txt" name="log" label="${title}_Log" />
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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100 </outputs>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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101
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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102 <tests>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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103 <test maxseconds="3600" >
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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104 <param name="selection" value="classicGSEA" />
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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105 <param name="expressionData" value="./NormalizedData.tabular" />
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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106 <param name="conditionInformation" value="./conditionGroups.txt" />
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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107 <param name="factorToInclude" value="Treatment" />
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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108 <output name="outExpression" file="./GSEA-Formatting/outputExpression.gct" />
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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109 <output name="outPhenotypes" file="./GSEA-Formatting/outputPhenotypesTreatment.cls" />
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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110 <output name="log" file="./GSEA-Formatting/outputRanks.log" />
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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111 </test>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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112 <test maxseconds="3600" >
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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113 <param name="selection" value="classicGSEA" />
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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114 <param name="expressionData" value="./NormalizedData.tabular" />
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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115 <param name="conditionInformation" value="./conditionGroups.txt" />
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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116 <param name="factorToInclude" value="Type" />
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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117 <output name="outExpression" file="./GSEA-Formatting/outputExpression.gct" />
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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118 <output name="outPhenotypes" file="./GSEA-Formatting/outputPhenotypesType.cls" />
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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119 <output name="log" file="./GSEA-Formatting/outputRanks.log" />
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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120 </test>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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121 <test maxseconds="3600" >
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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122 <param name="selection" value="rankedGSEA" />
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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123 <param name="differentialAnalysis" value="./LIMMAstatistics.tabular" />
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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124 <param name="comparisonsToUse" value="WT*Control-KO*Control" />
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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125 <param name="rankingIndice" value="FC" />
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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126 <param name="pvalThreshold" value="0.05" />
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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127 <output name="outRankedGenes" file="./GSEA-Formatting/outputRanks.rnk" />
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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128 <output name="log" file="./GSEA-Formatting/outputRanks.log" />
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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129 </test>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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130 </tests>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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131 <help>
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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132 <![CDATA[
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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133 **What it does**
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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134
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135 Generate input files required for GSEA analysis.
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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136
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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137 -----
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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138
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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139 **Parameters**
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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140
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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141 \- **Title** to personalize output file names (please avoid special characters).
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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142
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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143 \- **GSEA configuration** : "GSEA analysis" requires expression dataset and phenotype label for each sample whereas "Pre-ranked GSEA Analysis" needs ranked list of samples extracted from differential analysis results.
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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144
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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145 - **GSEA Analysis**
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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146
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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147 \- **Expression tabular file** with samples as columns and genes as rows (header row contains sample names and first column gene identifiers).
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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148
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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149 ::
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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150
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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151 Conditions 157_(HuGene-2_0-st).CEL 156_(HuGene-2_0-st).CEL 155_(HuGene-2_0-st).CEL 154_(HuGene-2_0-st).CEL
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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152 DDX11L2 4.500872 4.429759 4.780281 4.996189
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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153 MIR1302-2 3.415065 3.520472 3.471503 3.567988
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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154 OR4F5 3.737956 3.011586 3.424494 3.497545
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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155 VWA1 5.189621 5.129595 4.806793 5.227014
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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156
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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157 \- **Factor information tabular file** with factors as columns and samples as rows (header row contains factor names and first column sample names).
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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158
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159 ::
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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160
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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161 Conditions Sex Treatment Reaction
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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162 138_(HuGene-2_0-st).CEL 1 TreatA Pos
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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163 148_(HuGene-2_0-st).CEL 0 NoTreat Pos
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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164 139_(HuGene-2_0-st).CEL 0 TreatB Neg
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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165 149_(HuGene-2_0-st).CEL 0 NoTreat Neg
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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166
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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167 \- **Reference factor** to use as phenotype in GSEA amongst available factors in factor information file.
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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168
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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169 - **Pre-ranked GSEA Analysis**
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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170
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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171 \- **Differential analysis tabular file** with contrasts statistics (p-val, FDR p-val, FC, log2(FC) and moderated t-statistic) as columns and genes as rows (first and second rows contain contrasts definition and first and second columns contain gene identifiers and functional informations). Please respect the GIANT-Differential Expression Analysis with LIMMA tool output format.
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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172
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173 ::
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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174
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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175 LIMMA comparison WT*Treat WT*Treat WT*Treat WT*Treat WT*Treat
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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176 Gene Info p-val FDR.p-val FC log2(FC) t-stat
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177 ARSD na 0.0057 0.41 0.8389 -0.2534 -5.175
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178 TTTY10 na 1.6e-07 0.0074 0.6403 -0.6432 -6.122
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179 MIR548AL na 0.072 0.2914 1.711 0.775 10.43
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180
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181
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182 \- **Reference contrast** from available contrasts in differential analysis file to use for gene ranking.
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183
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184 \- **Reference statistic** from reference contrast used to rank genes. Relative or absolute value of log2(FC) or moderated t-statistic is used to sort genes in a decreasing way.
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185
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186 \- **FDR p-val threshold** to discard genes with higher FDR p-val than specific threshold [0.05 recommended]. Genes with high FDR p-val are not significant for differential expression in reference contrast.
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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187
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188
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189 -----
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190
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191 **Outputs**
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192
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193 Depends on GSEA configuration:
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194
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195 - **GSEA Analysis**
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196
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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197 \- **phenotype file (.cls)** to use as "phenotype labels" file for GSEA.
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198
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199 \- **expression file (.gct)** to use as "expression dataset" file for GSEA.
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200
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201 - **Pre-ranked GSEA Analysis**
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202
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203 \- **pre-ranked file (.rnk)** to use as "ranked list" file for GSEA pre-ranked.
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204
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205 For all configurations:
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206
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207 \- **LOG file** containing information about execution. Useful especially if tool execution fails. Please attach this log file in any bug report.
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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208 ]]>
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209 </help>
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210
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211 <citations>
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212 <citation type="bibtex">@misc{vandel_jimmy_2018_1477870, author = {Vandel, J. and Gheeraert, C. and Eeckhoute, J. and Staels, B. and Lefebvre, P. and Dubois-Chevalier, J.}, title = {GIANT: Galaxy-based Interactive tools for ANalaysis of Transcriptomic data}, month = nov, year = 2018, doi = {10.5281/zenodo.1477870}, url = {https://doi.org/10.5281/zenodo.1477870}
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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213 }</citation>
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214
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215 <citation type="bibtex">@article {Subramanian15545,
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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216 author = {Subramanian, Aravind and Tamayo, Pablo and Mootha, Vamsi K. and Mukherjee, Sayan and Ebert, Benjamin L. and Gillette, Michael A. and Paulovich, Amanda and Pomeroy, Scott L. and Golub, Todd R. and Lander, Eric S. and Mesirov, Jill P.},
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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217 title = {Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles},
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218 volume = {102},
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219 number = {43},
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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220 pages = {15545--15550},
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221 year = {2005},
3022feec50fe "planemo upload for repository https://github.com/juliechevalier/GIANT/tree/master commit cb276a594444c8f32e9819fefde3a21f121d35df"
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222 publisher = {National Academy of Sciences},
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223 issn = {0027-8424},
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224 journal = {Proceedings of the National Academy of Sciences}
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225 }</citation>
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226 </citations>
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227
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228 </tool>