Mercurial > repos > vipints > fml_gff3togtf
diff gff_fmap.xml @ 5:6e589f267c14
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author | devteam |
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date | Tue, 04 Nov 2014 12:15:19 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/gff_fmap.xml Tue Nov 04 12:15:19 2014 -0500 @@ -0,0 +1,83 @@ +<tool id="fml_gfffmap" name="GFF-map" version="2.0.0"> + <description>features</description> + <command interpreter="python"> + gff_fmap.py $gff_input > $idmapping + </command> + <inputs> + <param format="gff3,gff" name="gff_input" type="data" label="Query file" help="Provide genome annotation file in GFF."/> + </inputs> + <outputs> + <data format="txt" name="idmapping" label="${tool.name} on ${on_string}: parent child id map"/> + </outputs> + <tests> + <test> + <param name="gff_input" value="Feature_ID_mapping_W.gff3" /> + <output name="idmapping" file="Feature_ID_mapping_W.txt" /> + </test> + <test> + <param name="gff_input" value="Aly_JGI.gff3" /> + <output name="idmapping" file="Feature_ID_mapping_R.txt" /> + </test> + </tests> + <help> + +**What it does** + +GFF-map provides the features (gene, mRNA, UTR's, exon, CDS etc) relationship based on their identifier mapping in a given GFF file. + +-------- + +**Example** + +- The features ID mapping in the following data in GFF3:: + + ##gff-version 3 + 17 protein_coding gene 7255208 7258258 . + . ID=ENSG00000213859;Name=KCTD11 + 17 protein_coding mRNA 7255208 7258258 . + . ID=ENST00000333751;Name=KCTD11-001;Parent=ENSG00000213859 + 17 protein_coding protein 7256262 7256960 . + 0 ID=ENSP00000328352;Name=ENSP00000328352 + 17 protein_coding five_prime_UTR 7255208 7256261 . + . Parent=ENST00000333751 + 17 protein_coding CDS 7256262 7256960 . + 0 Name=CDS:KCTD11;Parent=ENST00000333751,ENSP00000328352 + 17 protein_coding three_prime_UTR 7256961 7258258 . + . Parent=ENST00000333751 + 17 protein_coding exon 7255208 7258258 . + . Parent=ENST00000333751 + +- Will be displayed as:: + + +-----------------------+---------------------------------+ + | Parent feature type | Associated child feature type(s)| + +-----------------------+---------------------------------+ + | protein_coding gene | protein_coding mRNA | + +-----------------------+---------------------------------+ + | protein_coding protein| protein_coding CDS | + +-----------------------+---------------------------------+ + | protein_coding mRNA | protein_coding CDS | + | | protein_coding exon | + | | protein_coding five_prime_UTR | + | | protein_coding three_prime_UTR | + +-----------------------+---------------------------------+ + +-------- + +**About formats** + +**GFF3 format** General Feature Format is a format for describing genes and other features associated with DNA, RNA and Protein sequences. GFF3 lines have nine tab-separated fields:: + + 1. seqid - Must be a chromosome or scaffold. + 2. source - The program that generated this feature. + 3. type - The name of this type of feature. Some examples of standard feature types are "gene", "CDS", "protein", "mRNA", and "exon". + 4. start - The starting position of the feature in the sequence. The first base is numbered 1. + 5. stop - The ending position of the feature (inclusive). + 6. score - A score between 0 and 1000. If there is no score value, enter ".". + 7. strand - Valid entries include '+', '-', or '.' (for don't know/care). + 8. phase - If the feature is a coding exon, frame should be a number between 0-2 that represents the reading frame of the first base. If the feature is not a coding exon, the value should be '.'. + 9. attributes - All lines with the same group are linked together into a single item. + +-------- + +**Copyright** + +2009-2014 Max Planck Society, University of Tübingen & Memorial Sloan Kettering Cancer Center + +Sreedharan VT, Schultheiss SJ, Jean G, Kahles A, Bohnert R, Drewe P, Mudrakarta P, Görnitz N, Zeller G, Rätsch G. Oqtans: the RNA-seq workbench in the cloud for complete and reproducible quantitative transcriptome analysis. Bioinformatics 10.1093/bioinformatics/btt731 (2014) + +</help> +</tool>