Mercurial > repos > wolma > mimodd
diff cloudmap.xml @ 0:6231ae8f87b8
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author | wolma |
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date | Wed, 11 Feb 2015 08:29:02 -0500 |
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children | a548b3c6ed00 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/cloudmap.xml Wed Feb 11 08:29:02 2015 -0500 @@ -0,0 +1,70 @@ +<tool id="cloudmap_prepare" name="Prepare variant data for mapping"> + <description>with the CloudMap series of tools.</description> + <version_command>mimodd version -q</version_command> + <command> + mimodd cloudmap "$ifile" ${run.mode} + + #if $str($run.mode) != "SVD": + "${run.refsample}" + #end if + + "$sample" -o "$ofile" + + #if $seqdict: + -s "$dictfile" + #end if + </command> + + <inputs> + <param name="ifile" type="data" format="vcf" label="vcf input file" /> + <conditional name="run"> + <param name="mode" type="select" label="CloudMap analysis to prepare data for"> + <option value="SVD">EMS Variant Density Mapping</option> + <option value="VAF">Variant Discovery / Hawaiian Variant Mapping</option> + </param> + <when value="SVD"> + <param name="refsample" type="hidden" value="None" /> + </when> + <when value="VAF"> + <param name="refsample" type="text" label="name of the reference sample" help="the sample that provides mapping strain variants" /> + </when> + </conditional> + <param name="sample" type="text" label="subject sample name" help="the sample to perform CloudMap mapping for" /> + <param name="seqdict" type="boolean" checked="true" label="Generate species configuration file for CloudMap" /> + + </inputs> + + <outputs> + <data name="ofile" format="vcf" label="CloudMap-ready ${run.mode} File from ${on_string}" /> + <data name="dictfile" format="tabular" label="Species Configuration File for CloudMap from ${on_string}"> + <filter>seqdict</filter> + </data> + </outputs> + + <help> +.. class:: infomark + + **What it does** + +The purpose of this tool is to provide compatibility of the MiModD analysis workflow with the external `CloudMap`_ *EMS Variant Density Mapping*, *Variant Discovery Mapping* and *Hawaiian Variant Mapping* tools. + +These tools complement MiModD by providing easily interpreted visualizations of mapping-by-sequencing analysis workflows. + +The tool converts a VCF file as generated by the *Extract Variant Sites* or *VCF Filter* tools to the format expected by the *CloudMap* series of tools. + +Optionally, it also extracts the chromosome names and sizes and reports them in the *CloudMap* *species configuration file* format. +Such a file is required as input to the current versions of the *CloudMap* *Hawaiian* and *Variant Density* mapping tools, if you are working with a species other than the natively supported ones (i.e., other than *C. elegans* or *A. thaliana*). + +To use the output datasets of the tool with *CloudMap*, you only have to upload them to any public Galaxy server that hosts *CloudMap* like, e.g., the main Galaxy server at https://usegalaxy.org . + +.. class:: warningmark + + EMS Variant Density Mapping is currently limited to *C. elegans* and other species with six chromosomes on the *CloudMap* side. + +More information on combining MiModD and CloudMap in mapping-by-sequencing analyses can be found in the `corresponding section of the MiModD User Guide`_. + +.. _CloudMap: https://usegalaxy.org/u/gm2123/p/cloudmap +.. _corresponding section of the MiModD User Guide: http://mimodd.readthedocs.org/en/latest/cloudmap.html + + </help> +</tool>