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author | wolma |
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date | Thu, 21 Jul 2016 03:56:19 -0400 |
parents | 5db0545b9004 |
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<tool id="bamsort" name="Sort BAM file" version="0.1.7.3"> <description>Sort a BAM file by coordinates (or names) of the mapped reads</description> <macros> <import>toolshed_macros.xml</import> </macros> <expand macro="requirements" /> <version_command>python3 -m MiModD version -q</version_command> <command> python3 -m MiModD sort "$input.ifile" -o "$output" --iformat $input.iformat --oformat $oformat $by_name </command> <inputs> <conditional name="input"> <param label="Input data format" name="iformat" type="select"> <option value="bam">bam</option> <option value="sam">sam</option> </param> <when value="bam"> <param format="bam" label="BAM input file to sort" name="ifile" type="data" /> </when> <when value="sam"> <param format="sam" label="SAM input file to sort" name="ifile" type="data" /> </when> </conditional> <param label="Output format for the sorted data" name="oformat" type="select"> <option value="bam">bam</option> <option value="sam">sam</option> </param> <param checked="false" falsevalue="" help="A less common option, but necessary, e.g., if you want to re-align sorted output from a previous run of the Snap Align Tool." label="Sort by read names instead of coordinates" name="by_name" truevalue="-n" type="boolean" /> </inputs> <outputs> <data format="bam" label="Sorted output from MiModd ${tool.name} on ${on_string}" name="output"> <change_format> <when format="sam" input="oformat" value="sam" /> </change_format> </data> </outputs> <help> .. class:: infomark **What it does** The tool sorts a BAM file of aligned reads, typically by the reference genome coordinates that the reads have been mapped to. Coordinate-sorted input files are expected by most downstream MiModD tools, but note that the *SNAP Read Alignment* produces coordinate-sorted output by default and it is only necessary to sort files that come from other sources or from *SNAP Read Alignment* jobs with a custom sort order. The option *Sort by read names instead of coordinates* is useful if you want to re-align coordinate-sorted paired-end data. In *paired-end mode*, the *SNAP Read Alignment* tool expects the reads in the input file to be arranged in read pairs, i.e., the forward read information of a pair must be followed immediately by its reverse mate information, which is typically not the case in coordinate-sorted files. Resorting such files by read names fixes this problem. </help> </tool>