Mercurial > repos > yating-l > bamtobigwig
changeset 0:61f39c77b13d draft
planemo upload commit 250051c3d660df43da92c76913552767ce142a45
author | yating-l |
---|---|
date | Wed, 12 Apr 2017 17:52:09 -0400 |
parents | |
children | 2e812b4f91ce |
files | LICENSE README.md bam_to_bigwig.xml tool_dependencies.xml |
diffstat | 4 files changed, 78 insertions(+), 0 deletions(-) [+] |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/LICENSE Wed Apr 12 17:52:09 2017 -0400 @@ -0,0 +1,21 @@ +The MIT License (MIT) + +Copyright (c) 2016 RĂ©mi Marenco + +Permission is hereby granted, free of charge, to any person obtaining a copy +of this software and associated documentation files (the "Software"), to deal +in the Software without restriction, including without limitation the rights +to use, copy, modify, merge, publish, distribute, sublicense, and/or sell +copies of the Software, and to permit persons to whom the Software is +furnished to do so, subject to the following conditions: + +The above copyright notice and this permission notice shall be included in all +copies or substantial portions of the Software. + +THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR +IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, +FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE +AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER +LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, +OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE +SOFTWARE.
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/README.md Wed Apr 12 17:52:09 2017 -0400 @@ -0,0 +1,2 @@ +# bamToBigwig +Galaxy wrapper for Bam to Bigwig conversion
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/bam_to_bigwig.xml Wed Apr 12 17:52:09 2017 -0400 @@ -0,0 +1,30 @@ +<tool id="bam_to_bigwig" name="Convert Bam to BigWig" version="0.1.0"> + <requirements> + <requirement type="package" version="1.0">ucsc_tools_340</requirement> + <requirement type="package" version="2.24">bedtools</requirement> + </requirements> + <stdio> + <exit_code range="1:" /> + </stdio> + <command><![CDATA[ + faToTwoBit $input_reference_fasta two_bit.2bit; + twoBitInfo two_bit.2bit stdout | sort -k2nr > chrom.sizes; + bedtools genomecov -bg -split -ibam $input_bam -g chrom.sizes > temp.bg; + bedGraphToBigWig temp.bg chrom.sizes $output_bigwig; + ]]></command> + <inputs> + <param format="bam" name="input_bam" type="data" label="Choose BAM file" /> + <param format="fasta" name="input_reference_fasta" + type="data" label="Choose Reference Genome (Fasta)" /> + </inputs> + <outputs> + <data format="bigwig" name="output_bigwig" /> + </outputs> + <help> + This tool converts a Bam file to a BigWig file. + + It needs: + - A Bam file + - The reference genome as a Fasta file + </help> +</tool> \ No newline at end of file
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool_dependencies.xml Wed Apr 12 17:52:09 2017 -0400 @@ -0,0 +1,25 @@ +<?xml version="1.0"?> +<tool_dependency> + <package name="ucsc_tools_340" version="1.0"> + <install version="1.0"> + <actions_group> + <actions architecture="x86_64" os="linux"> + <action type="download_by_url">http://old-gep.wustl.edu/~galaxy/ucsc_tools_340.tar.gz</action> + <action type="move_directory_files"> + <source_directory>.</source_directory> + <destination_directory>$INSTALL_DIR/bin</destination_directory> + </action> + </actions> + <action type="set_environment"> + <environment_variable action="prepend_to" name="PATH">$INSTALL_DIR/bin</environment_variable> + </action> + </actions_group> + </install> + <readme>The well known UCSC tools from Jim Kent.</readme> + </package> + + <package name="bedtools" version="2.24"> + <repository changeset_revision="3416a1d4a582" name="package_bedtools_2_24" owner="iuc" prior_installation_required="True" toolshed="https://toolshed.g2.bx.psu.edu" /> + </package> + +</tool_dependency>