Mercurial > repos > yating-l > jbrowsearchivecreator

annotate blastxmlToGff3.py @ 0:804a93e87cc8 draft

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planemo upload for repository https://github.com/Yating-L/jbrowse_hub commit f22711ea7a464bdaf4d5aaea07f2eacf967aa66e-dirty
author yating-l
date Wed, 12 Apr 2017 17:41:55 -0400
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0
804a93e87cc8 planemo upload for repository https://github.com/Yating-L/jbrowse_hub commit f22711ea7a464bdaf4d5aaea07f2eacf967aa66e-dirty
yating-l
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1 #!/usr/bin/env python
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yating-l
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2
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3
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yating-l
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4 from Bio.Blast import NCBIXML
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yating-l
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5 from collections import OrderedDict
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6 import utils
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7
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8
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9 def align2cigar(hsp_query, hsp_reference):
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10 """
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11 Build CIGAR representation from an hsp_query
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12 input:
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13 hsp_query
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14 hsp_sbjct
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15 output:
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16 CIGAR string
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17 """
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18 query = hsp_query
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19 ref = hsp_reference
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20 # preType, curType:
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21 # 'M' represents match,
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22 # 'I' represents insert a gap into the reference sequence,
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23 # 'D' represents insert a gap into the target (delete from reference)
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24 # some ideas of this algin2cigar function are coming from
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25 # https://gist.github.com/ozagordi/099bdb796507da8d9426
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26 prevType = 'M'
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27 curType = 'M'
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28 count = 0
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29 cigar = []
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30 num = len(query)
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31 for i in range(num):
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32 if query[i] == '-':
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33 curType = 'D'
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34 elif ref[i] == '-':
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35 curType = 'I'
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36 else:
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37 curType = 'M'
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38 if curType == prevType:
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39 count += 1
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40 else:
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41 cigar.append('%s%d' % (prevType, count))
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yating-l
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42 prevType = curType
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43 count = 1
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44 cigar.append('%s%d' % (curType, count))
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45 return ' '.join(cigar)
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46
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47 def gff3_writer(blast_records, gff3_file):
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48 gff3 = open(gff3_file, 'a')
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49 gff3.write("##gff-version 3\n")
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50 seq_regions = dict()
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51 for blast_record in blast_records:
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52 query_name = blast_record.query.split(" ")[0]
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53 source = blast_record.application
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54 method = blast_record.matrix
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55 for alignment in blast_record.alignments:
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56 group = {
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57 "parent_field" : OrderedDict(),
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58 "parent_attribute" : OrderedDict(),
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59 "alignments" : []
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60 }
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61 title = alignment.title.split(" ")
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62 contig_name = title[len(title) - 1]
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63 length = alignment.length
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64 group['parent_field']['seqid'] = contig_name
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65 group['parent_field']['source'] = source
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66 group['parent_field']['type'] = 'match'
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67 group['parent_attribute']['ID'] = contig_name + '_' + query_name
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68 group['parent_attribute']['method'] = method
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69 group['parent_attribute']['length'] = length
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70 if contig_name not in seq_regions:
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71 gff3.write("##sequence-region " + contig_name + ' 1 ' + str(length) + '\n')
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72 seq_regions[contig_name] = length
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73 match_num = 0
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74 coords = [length, 0]
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75 for hsp in alignment.hsps:
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76 hsp_align = {}
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77 field = OrderedDict()
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78 attribute = OrderedDict()
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79 ref = hsp.sbjct
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80 query = hsp.query
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81 field['seqid'] = contig_name
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82 field['source'] = source
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83 field['type'] = 'match_part'
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84
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85 field['start'] = hsp.sbjct_start
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86 if field['start'] < coords[0]:
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87 coords[0] = field['start']
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88 ref_length = len(ref.replace('-', ''))
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89 # if run tblastn, the actual length of reference should be multiplied by 3
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90 if source.lower() == "tblastn":
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91 ref_length *= 3
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92 field['end'] = field['start'] + ref_length - 1
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93 if field['end'] > coords[1]:
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94 coords[1] = field['end']
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95 field['score'] = hsp.score
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96 #decide if the alignment in the same strand or reverse strand
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97 #reading frame
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98 # (+, +), (0, 0), (-, -) => +
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99 # (+, -), (-, +) => -
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100 if hsp.frame[1] * hsp.frame[0] > 0:
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101 field['strand'] = '+'
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102 elif hsp.frame[1] * hsp.frame[0] < 0:
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103 field['strand'] = '-'
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104 else:
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105 if hsp.frame[0] + hsp.frame[1] >= 0:
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106 field['strand'] = '+'
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107 else:
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108 field['strand'] = '-'
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109 field['phase'] = '.'
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110
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111 target_start = hsp.query_start
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112 target_len = len(query.replace('-', ''))
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113 # if run blastx, the actual length of query should be multiplied by 3
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114 if source.lower() == "blastx":
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115 target_len *= 3
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116 target_end = target_start + target_len -1
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117 attribute['ID'] = group['parent_attribute']['ID'] + '_match_' + str(match_num)
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118 attribute['Parent'] = group['parent_attribute']['ID']
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119 attribute['Target'] = query_name + " " + str(target_start) + " " + str(target_end)
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120 attribute['Gap'] = align2cigar(query, ref)
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121 #store the query sequence and match string in the file in order to display alignment with BlastAlignment plugin
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122 attribute['subject'] = hsp.sbjct
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123 attribute['query'] = hsp.query
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124 attribute['match'] = hsp.match
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125 attribute['gaps'] = attribute['match'].count(' ')
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126 similar = attribute['match'].count('+')
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127 attribute['identities'] = len(attribute['match']) - similar - attribute['gaps']
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128 attribute['positives'] = attribute['identities'] + similar
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129 attribute['expect'] = hsp.expect
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130 # show reading frame attribute only if the frame is not (0, 0)
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131 attribute['frame'] = hsp.frame[1]
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132 match_num += 1
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133 hsp_align['field'] = field
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134 hsp_align['attribute'] = attribute
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135 group['alignments'].append(hsp_align)
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136 group['parent_field']['start'] = coords[0]
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137 group['parent_field']['end'] = coords[1]
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138 group['parent_field']['score'] = group['parent_field']['strand'] = group['parent_field']['phase'] = '.'
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139 group['parent_attribute']['match_num'] = match_num
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140 group['alignments'].sort(key=lambda x: (x['field']['start'], x['field']['end']))
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141 utils.write_features(group['parent_field'], group['parent_attribute'], gff3)
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142 prev_end = -1
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143 for align in group['alignments']:
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144 overlap = ''
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145 if align['field']['start'] <= prev_end:
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146 overlap += str(align['field']['start']) + ',' + str(prev_end)
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147 prev_end = align['field']['end']
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148 align['attribute']['overlap'] = overlap
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149 utils.write_features(align['field'], align['attribute'], gff3)
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150 gff3.close()
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151
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152 def blastxml2gff3(xml_file, gff3_file):
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153 result_handle = open(xml_file)
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154 blast_records = NCBIXML.parse(result_handle)
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155 gff3_writer(blast_records, gff3_file)
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156
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157 if __name__ == "__main__":
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158 blastxml2gff3("../dbia3/raw/tblastn_dmel-hits-translation-r6.11.fa_vs_nucleotide_BLAST_database_from_data_3.blastxml", "gff3.txt")
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159