Mercurial > repos > yating-l > ucsc_blat
annotate blat.xml @ 5:dede60f744f4 draft
planemo upload commit 2d70129e3c77058bd09e7b0da426b5ee3187cce7
author | iuc |
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date | Fri, 23 Jun 2017 23:33:09 -0400 |
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children | 250a7f2b1331 |
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1 <?xml version="1.0"?> |
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2 <tool id="ucsc_blat" name="UCSC BLAT Alignment Tool" version="35-0"> |
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3 <description>BLAST-like sequence alignment tool</description> |
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4 <requirements> |
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5 <requirement type="package" version="35">blat</requirement> |
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6 </requirements> |
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7 <command detect_errors="exit_code"><![CDATA[ |
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8 #if str($reference_source.reference_source_selector) == "history": |
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9 ## blat depends on file extension |
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10 #if $reference_source.database.is_of_type("fasta"): |
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11 #set $reference_fasta_filename = "localref.fa" |
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12 #elif $reference_source.database.is_of_type("twobit"): |
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13 #set $reference_fasta_filename = "localref.2bit" |
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14 #else |
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15 #set $reference_fasta_filename = "localref" |
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16 #end if |
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17 |
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18 ln -s '${reference_source.database}' '${reference_fasta_filename}' && |
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19 #else: |
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20 #set $reference_fasta_filename = str($reference_source.database.fields.path) |
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21 #end if |
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22 |
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23 blat |
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24 #if $noHead == "yes" |
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25 -noHead |
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26 #end if |
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27 |
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28 -q=$query_type |
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29 -t=$database_type |
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30 |
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31 #if $mask_type.mask == "file.out": |
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32 -mask='${mask_type.mask_file}' |
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33 #else: |
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34 -mask=$mask_type.mask |
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35 #end if |
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36 |
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37 '$reference_fasta_filename' |
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38 '${query}' |
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39 |
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40 output |
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41 && sort -k 10,10 -k 12,12n output > '${output_sorted}' |
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42 ]]></command> |
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43 <inputs> |
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44 <conditional name="reference_source"> |
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45 <param name="reference_source_selector" type="select" label="Choose the source for the database"> |
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46 <option value="cached">Locally cached</option> |
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47 <option value="history">History</option> |
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48 </param> |
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49 <when value="cached"> |
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50 <param name="database" type="select" label="Select database"> |
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51 <options from_data_table="all_fasta"> |
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52 <filter type="sort_by" column="2" /> |
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53 </options> |
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54 <validator type="no_options" message="A built-in database is not available" /> |
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55 </param> |
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56 </when> |
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57 <when value="history"> |
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58 <param name="database" type="data" format="fasta, twobit" label="Using database file, either a .fa, .nib or .2bit file" /> |
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59 </when> |
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60 </conditional> |
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61 <param type="data" name="query" format="fasta, twobit" label="Query data, either a .fa, .nib or .2bit file"/> |
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62 <param type="select" name="database_type" format="text" multiple="false" label="database type" help="Choose your database type, the default is dnax" argument="-t"> |
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63 <option value="dna">dna - DNA sequence</option> |
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64 <option value="prot">prot - protein sequence</option> |
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65 <option value="dnax" selected="true">dnax - DNA sequence translated in six frames to protein</option> |
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66 </param> |
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67 <param type="select" name="query_type" format="text" multiple="false" label="query type" help="Choose your query type, the default is rnax" argument="-q"> |
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68 <option value="dna">dna - DNA sequence </option> |
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69 <option value="rna">rna - RNA sequence</option> |
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70 <option value="prot">prot - protein sequence</option> |
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71 <option value="dnax">dnax - DNA sequence translated in six frames to protein</option> |
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72 <option value="rnax" selected="true">rnax - DNA sequence translated in three frames to protein</option> |
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73 </param> |
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74 <param type="select" name="noHead" format="text" label="Suppresses .psl header (so it's just a tab-separated file)." > |
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75 <option value="yes" selected="true">Yes, suppresses .psl header</option> |
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76 <option value="no">No, do not suppresses .psl header</option> |
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77 </param> |
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78 <conditional name="mask_type"> |
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79 <param name="mask" type="select" label="Mask out repeats" help="Alignments won't be started in masked region |
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80 but may extend through it in nucleotide searches. Masked areas |
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81 are ignored entirely in protein or translated searches. Default is lower" argument="-mask"> |
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82 <option value="lower" selected="true">lower - mask out lower-cased sequence</option> |
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83 <option value="upper">upper - mask out upper-cased sequence</option> |
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84 <option value="out">out - mask according to database.out RepeatMasker .out file</option> |
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85 <option value="file.out">file.out - mask database according to RepeatMasker file.out</option> |
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86 </param> |
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87 <when value="lower" /> |
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88 <when value="upper" /> |
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89 <when value="out" /> |
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90 <when value="file.out"> |
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91 <param name="mask_file" type="data" format="txt" label="RepeatMasker file.out" /> |
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92 </when> |
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93 </conditional> |
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94 </inputs> |
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95 <outputs> |
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96 <data format="psl" name="output_sorted" label="${tool.name} on ${on_string}"></data> |
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97 </outputs> |
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98 <tests> |
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99 <!-- test on query of GenBank RefSeq records for Gallus gallus and database of Amazona vittata --> |
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100 <test> |
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101 <param name="reference_source_selector" value="history" /> |
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102 <param name="database" value="amaVit1_Gallus/amaVit1.fa" /> |
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103 <param name="query" value="amaVit1_Gallus/Gallus_gallus_RefSeq.fa" /> |
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104 <param name="database_type" value="dnax" /> |
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105 <param name="query_type" value="rnax" /> |
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106 <param name="noHead" value="yes" /> |
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107 <param name="mask" value="lower" /> |
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108 <output name="output_sorted" value="amaVit1_Gallus/amaVit1_Gallus_gallus.psl" /> |
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109 </test> |
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110 <!-- test on query of partial mRNA of Drosophila melanogaster and the database of Drosophila biamipes dot chromosome --> |
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111 <test> |
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112 <param name="reference_source_selector" value="history" /> |
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113 <param name="database" value="dbia3/dbia3.fa" /> |
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114 <param name="query" value="dbia3/dmel-transcript.fa" /> |
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115 <param name="database_type" value="dnax" /> |
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116 <param name="query_type" value="rnax" /> |
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117 <param name="noHead" value="yes" /> |
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118 <param name="mask" value="lower" /> |
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119 <output name="output_sorted" value="dbia3/dbia3.sorted.psl" /> |
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120 </test> |
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121 <!-- test on the database masked by repeat masker --> |
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122 <test> |
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123 <param name="reference_source_selector" value="history" /> |
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124 <param name="database" value="dbia3/dbia3_masked.2bit" /> |
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125 <param name="query" value="dbia3/dmel-transcript.fa" /> |
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126 <param name="database_type" value="dnax" /> |
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127 <param name="query_type" value="rnax" /> |
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128 <param name="noHead" value="yes" /> |
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129 <param name="mask" value="file.out" /> |
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130 <param name="mask_file" value="dbia3/dbia3_RM.out" /> |
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131 <output name="output_sorted" value="dbia3/dbia3_masked.sorted.psl" /> |
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132 </test> |
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133 |
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134 </tests> |
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135 <help> |
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136 <![CDATA[ |
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137 BLAT |
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138 ==== |
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139 BLAT is a bioinformatics software a tool which performs rapid mRNA/DNA and cross-species protein alignments. |
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140 |
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141 blat (version: v340)- Standalone blat sequence search command line tool. |
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142 ------------------------------------------------------------------------- |
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143 |
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144 usage: |
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145 ++++++ |
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146 |
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147 $ blat database query [-ooc=11.ooc] output.psl |
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148 |
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149 where: |
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150 database and query are each either a .fa, .nib or .2bit file, |
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151 or a list of these files with one file name per line. |
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152 -ooc=11.ooc tells the program to load over-occurring 11-mers from |
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153 an external file. This will increase the speed |
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154 by a factor of 40 in many cases, but is not required. |
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155 output.psl is the name of the output file. |
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156 |
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157 documentation: |
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158 ++++++++++++++ |
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159 |
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160 See Blat documentation (http://genome.ucsc.edu/goldenPath/help/blatSpec.html) |
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161 |
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162 Source code: |
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163 ++++++++++++ |
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164 |
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165 http://hgdownload.cse.ucsc.edu/admin/exe/ |
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166 |
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167 ]]></help> |
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168 <citations> |
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169 <citation type="doi">10.1101/gr.229202</citation> |
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170 </citations> |
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171 </tool> |
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172 |
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173 |
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174 |
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175 |
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176 |