Mercurial > repos > bgruening > deeptools_plot_correlation
annotate plotCorrelation.xml @ 29:2c66e43f1c86 draft
planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 810c1359b884661d3f5c7372be0f0f88c859a3b9
author | bgruening |
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date | Thu, 01 Jun 2023 14:49:25 +0000 |
parents | 32e451def88c |
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29
2c66e43f1c86
planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 810c1359b884661d3f5c7372be0f0f88c859a3b9
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1 <tool id="deeptools_plot_correlation" name="plotCorrelation" version="@TOOL_VERSION@+galaxy0" profile="@GALAXY_VERSION@"> |
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ed2767cdf4e8
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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2 <description>Create a heatmap or scatterplot of correlation scores between different samples </description> |
ed2767cdf4e8
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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3 <macros> |
ed2767cdf4e8
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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4 <token name="@BINARY@">plotCorrelation</token> |
ed2767cdf4e8
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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5 <import>deepTools_macros.xml</import> |
ed2767cdf4e8
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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6 </macros> |
ed2767cdf4e8
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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7 <expand macro="requirements"/> |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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8 <command> |
ed2767cdf4e8
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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9 <![CDATA[ |
ed2767cdf4e8
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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10 @BINARY@ |
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planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 5a01e3a370130f32a82d5436504371ed95be1754
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11 --corData '$corData' |
4e8b35fd2173
planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 5a01e3a370130f32a82d5436504371ed95be1754
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12 --plotFile '$outFileName' |
4e8b35fd2173
planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 5a01e3a370130f32a82d5436504371ed95be1754
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13 --corMethod '$corMethod' |
4e8b35fd2173
planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 5a01e3a370130f32a82d5436504371ed95be1754
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14 --whatToPlot '$plotting_type.whatToPlot' |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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15 #if str($plotting_type.whatToPlot) == 'heatmap': |
ed2767cdf4e8
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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16 @HEATMAP_OPTIONS@ |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b5fc4073c884a2d6a4092ea85150db1971010f28
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17 --plotWidth $plotting_type.plotWidth |
d40bd147adf3
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit b5fc4073c884a2d6a4092ea85150db1971010f28
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18 --plotHeight $plotting_type.plotHeight |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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19 #else: |
ed2767cdf4e8
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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20 --plotTitle '$plotting_type.plotTitle' |
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32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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21 #if str($plotting_type.xrange_conditional.xrange_select) == 'yes': |
32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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22 --xRange $plotting_type.xrange_conditional.xRange_min $plotting_type.xrange_conditional.xRange_max |
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planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 38cfe39e3b3c82bbc0c2013e3068bd71adc3a9cb
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23 #end if |
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32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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24 #if str($plotting_type.yrange_conditional.yrange_select) == 'yes': |
32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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25 --yRange $plotting_type.yrange_conditional.yRange_min $plotting_type.yrange_conditional.yRange_max |
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planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit b1f975422b307927bbbe245d57609e9464d5d5c8-dirty
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26 #end if |
90d5df0a49bd
planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit b1f975422b307927bbbe245d57609e9464d5d5c8-dirty
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27 $plotting_type.log1p |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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28 #end if |
ed2767cdf4e8
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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29 $skipZeros |
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4e8b35fd2173
planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 5a01e3a370130f32a82d5436504371ed95be1754
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30 --plotFileFormat '$outFileFormat' |
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ed2767cdf4e8
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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31 $removeOutliers |
ed2767cdf4e8
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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32 #if $outFileCorMatrix: |
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4e8b35fd2173
planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 5a01e3a370130f32a82d5436504371ed95be1754
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33 --outFileCorMatrix '$matrix' |
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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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34 #end if |
ed2767cdf4e8
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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35 |
ed2767cdf4e8
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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36 ]]> |
ed2767cdf4e8
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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37 </command> |
ed2767cdf4e8
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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38 <inputs> |
ed2767cdf4e8
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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39 <param name="corData" format="deeptools_coverage_matrix" type="data" label="Matrix file from the multiBamSummary tool"/> |
ed2767cdf4e8
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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40 <expand macro="corMethod" /> |
ed2767cdf4e8
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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41 |
ed2767cdf4e8
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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42 <conditional name="plotting_type" > |
ed2767cdf4e8
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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43 <param argument="--whatToPlot" type="select" label="Plotting type"> |
ed2767cdf4e8
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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44 <option value="heatmap" selected="True">Heatmap</option> |
ed2767cdf4e8
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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45 <option value="scatterplot">Scatterplot</option> |
ed2767cdf4e8
planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 09975f870c75347fba5c6777c9f3b442bdeeb289
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46 </param> |
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47 <when value="heatmap"> |
ed2767cdf4e8
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48 <expand macro="heatmap_options" /> |
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49 <expand macro="plotWidthHeight" PLOTWIDTH="11.0" PLOTHEIGHT="9.5" /> |
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50 </when> |
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51 <when value="scatterplot"> |
ed2767cdf4e8
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52 <expand macro="plotTitle" /> |
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32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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53 <conditional name="xrange_conditional"> |
32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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54 <param type="select" name="xrange_select" label="Manually set X axis range?"> |
32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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55 <option value="no" selected="True">No</option> |
32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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56 <option value="yes">Yes</option> |
32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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57 </param> |
32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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58 <when value="yes"> |
32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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59 <param name="xRange_min" type="integer" value="0" label="X axis minimum value" |
32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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60 help="X axis minimum value. Not specifying this scales these such that the full range of dots is displayed." /> |
32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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61 <param name="xRange_max" type="integer" value="1000" label="X axis maximum value" |
32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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62 help="X axis maximum value. Not specifying this scales these such that the full range of dots is displayed." /> |
32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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63 </when> |
32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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64 <when value="no" /> |
32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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65 </conditional> |
32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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66 <conditional name="yrange_conditional"> |
32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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67 <param type="select" name="yrange_select" label="Manually set Y axis range?"> |
32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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68 <option value="no" selected="True">No</option> |
32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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69 <option value="yes">Yes</option> |
32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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70 </param> |
32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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71 <when value="yes"> |
32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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72 <param name="yRange_min" type="integer" value="0" label="Y axis minimum value" |
32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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73 help="Y axis minimum value. Not specifying this scales these such that the full range of dots is displayed." /> |
32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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74 <param name="yRange_max" type="integer" value="1000" label="Y axis maximum value" |
32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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75 help="Y axis maximum value. Not specifying this scales these such that the full range of dots is displayed." /> |
32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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76 </when> |
32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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77 <when value="no" /> |
32e451def88c
"planemo upload for repository https://github.com/deeptools/deepTools/tree/master/galaxy/wrapper/ commit 7c89456b6a986259c96b8d280c53e4ab155696ef"
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78 </conditional> |
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79 <param argument="--log1p" type="boolean" truevalue="--log1p" falsevalue="" |
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80 label="Log transform" |
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81 help="Plot the natural log of the scatter plot after adding 1. Note that this is ONLY for plotting, the correlation is unaffected." /> |
12
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82 </when> |
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83 </conditional> |
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84 |
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85 <expand macro="skipZeros" /> |
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86 |
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87 <expand macro="input_image_file_format" /> |
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88 |
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89 <param argument="--removeOutliers" type="boolean" |
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90 truevalue="--removeOutliers" falsevalue="" label="Remove regions with very large counts" |
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91 help="If set, bins with very large counts are removed. Bins |
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92 with abnormally high reads counts artificially |
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93 increase pearson correlation; that's why, by default, |
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94 plotCorrelation tries to remove outliers using the median |
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95 absolute deviation (MAD) method applying a threshold |
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96 of 200 to only consider extremely large deviations |
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97 from the median. ENCODE blacklist page (https://sites. |
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98 google.com/site/anshulkundaje/projects/blacklists) |
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99 contains useful information about regions with |
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100 unusually high counts."/> |
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101 |
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102 <param name="outFileCorMatrix" type="boolean" label="Save the matrix of values underlying the heatmap"/> |
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103 |
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104 </inputs> |
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105 <outputs> |
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106 <expand macro="output_image_file_format_not_nested" /> |
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107 <data format="tabular" name="matrix" label="${tool.name} on ${on_string}: Correlation matrix"> |
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108 <filter>outFileCorMatrix is True</filter> |
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109 </data> |
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110 </outputs> |
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111 <tests> |
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112 <test> |
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113 <param name="corData" value="multiBamSummary_result1.npz" ftype="deeptools_coverage_matrix" /> |
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114 <param name="outFileFormat" value="png" /> |
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115 <param name="outFileCorMatrix" value="True" /> |
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116 <output name="matrix" file="plotCorrelation_result1.tabular" ftype="tabular" /> |
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117 <output name="outFileName" file="plotCorrelation_result1.png" ftype="png" compare="sim_size" delta="400" /> |
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118 </test> |
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119 <test> |
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120 <param name="corData" value="multiBamSummary_result1.npz" ftype="deeptools_coverage_matrix" /> |
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121 <param name="outFileFormat" value="png" /> |
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122 <param name="whatToPlot" value="scatterplot" /> |
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123 <param name="removeOutliers" value="True" /> |
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124 <param name="plotTitle" value="Test Plot" /> |
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125 <output name="outFileName" file="plotCorrelation_result2.png" ftype="png" compare="sim_size" delta="300" /> |
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126 </test> |
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127 </tests> |
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128 <help> |
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129 <![CDATA[ |
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130 What it does |
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131 ------------ |
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132 |
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133 This tools takes the default output of ``multiBamSummary`` or ``multiBigwigSummary``, and computes the pairwise correlation among samples. |
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134 Results can be visualized as **scatterplots** or as a **heatmap** of correlation coefficients (see below for examples). |
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135 |
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136 Theoretical Background |
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137 ---------------------- |
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138 |
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139 The result of the correlation computation is a **table of correlation coefficients** that indicates how "strong" the relationship between two samples is and it will consist of numbers between -1 and 1. (-1 indicates perfect anti-correlation, 1 perfect correlation.) |
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140 |
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141 We offer two different functions for the correlation computation: *Pearson* or *Spearman*. |
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142 |
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143 The *Pearson method* measures the **metric differences** between samples and is therefore influenced by outliers. |
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144 The *Spearman method* is based on **rankings**. |
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145 |
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146 Output |
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147 ------ |
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148 |
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149 The default output is a **diagnostic plot** -- either a scatterplot or a clustered heatmap displaying the values for each pair-wise correlation (see below for example plots). |
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150 |
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151 Optionally, you can also obtain a table of the pairwise correlation coefficients. |
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152 |
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153 .. image:: $PATH_TO_IMAGES/plotCorrelation_output.png |
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154 :width: 600 |
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155 :height: 271 |
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156 |
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157 Example plots |
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158 ------------- |
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159 |
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160 The following is the output of ``plotCorrelation`` with our test ChIP-Seq datasets (to be found under "Shared Data" --> "Data Library"). |
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161 |
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162 Average coverages were computed over 10 kb bins for chromosome X, |
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163 from bigWig files using ``multiBigwigSummary``. This was then used with ``plotCorrelation`` to make a heatmap of Spearman correlation coefficients. |
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164 |
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165 .. image:: $PATH_TO_IMAGES/plotCorrelation_galaxy_bw_heatmap_output.png |
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166 :width: 600 |
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167 :height: 518 |
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168 |
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169 The scatterplot could look like this: |
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170 |
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171 .. image:: $PATH_TO_IMAGES/plotCorrelation_scatterplot_PearsonCorr_bigwigScores.png |
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172 :width: 600 |
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173 :height: 600 |
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174 |
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175 ----- |
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176 |
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177 @REFERENCES@ |
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178 ]]> |
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179 </help> |
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180 <expand macro="citations" /> |
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181 </tool> |