Mercurial > repos > bgruening > rnaz
annotate rnaz.xml @ 3:3c43015da1d8 draft
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit 2c2cc0e0638ebe372836509f5ff3d05a0bb34210-dirty
author | bgruening |
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date | Thu, 28 Sep 2017 11:57:06 -0400 |
parents | 580ee1e91801 |
children | 58fd61a8362e |
rev | line source |
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580ee1e91801
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e85474d72b07c70ca061f51b7165951038a06a6c
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1 <tool id="rnaz" name="RNAz" version="2.1.1"> |
0 | 2 <description>predicting structurally conserved and thermodynamically stable RNA secondary structures</description> |
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e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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3 |
0 | 4 <requirements> |
5 <requirement type="package" version="2.1">rnaz</requirement> | |
6 </requirements> | |
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e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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7 |
0 | 8 <stdio> |
9 <exit_code range="1:" level="fatal" description="Error occurred. Please check Tool Standard Error" /> | |
10 <exit_code range=":-1" level="fatal" description="Error occurred. Please check Tool Standard Error" /> | |
11 </stdio> | |
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e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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12 |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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13 <version_command>RNAz --version</version_command> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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14 <command> |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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15 <![CDATA[ |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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16 RNAz '$input' |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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17 --$forward_or_reverse |
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580ee1e91801
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e85474d72b07c70ca061f51b7165951038a06a6c
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18 $zscore |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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19 $locarnate |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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20 $noshuffle |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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21 #if $cutoff != -1.0: |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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22 --cutoff=$cutoff |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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23 #end if |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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24 > temp.txt && |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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25 grep -v -E "^ |^#|^$" temp.txt > '$outfile' && |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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26 grep -E "^ |^#|^$" temp.txt |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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27 ]]> |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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28 </command> |
0 | 29 <inputs> |
30 <param format="txt" name="input" type="data" label="Input Alignment File" /> | |
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e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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31 <param name="forward_or_reverse" type="select" label="Scored strand"> |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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32 <option value="forward">Score forward strand (-f)</option> |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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33 <option value="reverse">Score reverse strand (-r)</option> |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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34 <option value="both-strands">Score both strands (-b)</option> |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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35 </param> |
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580ee1e91801
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e85474d72b07c70ca061f51b7165951038a06a6c
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36 <param name="zscore" type="select" label="Which type of z-scores"> |
580ee1e91801
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e85474d72b07c70ca061f51b7165951038a06a6c
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37 <option value="--mononucleotide">Use mononucleotide shuffled z-scores</option> |
580ee1e91801
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e85474d72b07c70ca061f51b7165951038a06a6c
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38 <option value="--dinucleotide" selected="true">Use dinucleotide shuffled z-scores</option> |
580ee1e91801
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e85474d72b07c70ca061f51b7165951038a06a6c
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39 </param> |
1
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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40 <param argument="--cutoff" label="Probability cutoff" type="float" value="-1.0" help="-1.0 to deactivate"/> |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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41 <param argument="--locarnate" type="boolean" checked="false" truevalue="--locarnate" falsevalue="" label="Use decision model for structural alignments" /> |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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42 <param argument="--no-shuffle" name="noshuffle" type="boolean" checked="false" truevalue="--no-shuffle" falsevalue="" label="Never fall back to shuffling" /> |
0 | 43 </inputs> |
44 <outputs> | |
45 <data name="outfile" format="fasta" /> | |
46 </outputs> | |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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47 <tests> |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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48 <test> |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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49 <param name="input" value="rnaz_input_trna.aln"/> |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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50 <output name="outfile" file="rnaz_result_trna.fasta"/> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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51 </test> |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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52 <test> |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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53 <param name="input" value="rnaz_test_input2.aln"/> |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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54 <output name="outfile" file="rnaz_result2.fasta"/> |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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55 </test> |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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56 </tests> |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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57 <help> |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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58 <![CDATA[ |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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59 |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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60 **What it does** |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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61 |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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62 RNAz is a program for predicting structurally conserved and thermodynamically stable RNA secondary structures in multiple sequence alignments. It can be used in genome wide screens to detect functional RNA structures, as found in noncoding RNAs and cis-acting regulatory elements of mRNAs. |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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63 |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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64 **Input** |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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65 |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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66 Input is a multiple sequence alignment file. Currently the the following alignment formats can be read: CLUSTALW, FASTA, PHYLIP,NEXUS, MAF, and XMFA. Alignments can be generated by any sequence based alignment program. Sequence alignment tools can be found in Galaxy too (e.g. ClustalW). |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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67 |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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68 Example: |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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69 |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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70 CLUSTAL 2.1 multiple sequence alignment |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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71 |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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72 |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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73 sacCer1 GCCTTGTTGGCGCAATCGGTAGCGCGTATGACTCTTAATCATAAGGTTAGGGGTTCGAGC |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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74 |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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75 sacKlu GCCTTGTTGGCGCAATCGGTAGCGCGTATGACTCTTAATCATAAGGCTAGGGGTTCGAGC |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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76 |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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77 sacBay GCCTTGTTGGCGCAATCGGTAGCGCGTATGACTCTTAATCATAAGGTTAGGGGTTCGAGC |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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78 |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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79 sacCas GCTTCAGTAGCTCAGTCGGAAGAGCGTCAGTCTCATAATCTGAAGGTCGAGAGTTCGAAC |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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80 |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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81 \** * * \** \** \**\** \** \**\** * *\** \**\**\* *\**\* * \**\**\** * |
0 | 82 |
83 | |
1
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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84 **Output** |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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85 |
e23c455f8335
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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86 In Galaxy RNAz gives you 2 output files: a summary file and a result file. For the example input they look like this: |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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87 |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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88 Summary: |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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89 |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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90 Sequences: 4 |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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91 Columns: 60 |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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92 Reading direction: forward |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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93 Mean pairwise identity: 82.50 |
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planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rnaz commit e02ab52f82468dc7751e6564bdf6e959bbba3a65
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94 Shannon entropy: 0.28395 |
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95 G+C content: 0.51667 |
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96 Mean single sequence MFE: -16.67 |
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97 Consensus MFE: -15.59 |
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98 Energy contribution: -15.53 |
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99 Covariance contribution: -0.06 |
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100 Combinations/Pair: 1.26 |
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101 Mean z-score: -0.66 |
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102 Structure conservation index: 0.93 |
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103 Background model: mononucleotide |
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104 Decision model: sequence based alignment quality |
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105 SVM decision value: -0.64 |
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106 SVM RNA-class probability: 0.238023 |
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107 Prediction: OTHER |
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108 |
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109 |
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110 Result file: |
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111 |
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112 >sacCer1 |
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113 |
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114 GCCUUGUUGGCGCAAUCGGUAGCGCGUAUGACUCUUAAUCAUAAGGUUAGGGGUUCGAGC |
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115 |
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116 ..((((...((((........))))....(((((((((((....))))))))))))))). ( -19.00, z-score = -1.44, R) |
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117 |
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118 >sacKlu |
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119 |
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120 GCCUUGUUGGCGCAAUCGGUAGCGCGUAUGACUCUUAAUCAUAAGGCUAGGGGUUCGAGC |
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121 |
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122 ..((((...((((........))))....((((((((..(....)..)))))))))))). ( -16.00, z-score = -0.11, R) |
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123 |
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124 >sacBay |
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125 |
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126 GCCUUGUUGGCGCAAUCGGUAGCGCGUAUGACUCUUAAUCAUAAGGUUAGGGGUUCGAGC |
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127 |
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128 ..((((...((((........))))....(((((((((((....))))))))))))))). ( -19.00, z-score = -1.44, R) |
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129 |
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130 >sacCas |
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131 |
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132 GCUUCAGUAGCUCAGUCGGAAGAGCGUCAGUCUCAUAAUCUGAAGGUCGAGAGUUCGAAC |
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133 |
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134 .((((((..((((........))))..............)))))).(((......))).. ( -12.69, z-score = 0.35, R) |
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135 |
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136 >consensus |
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137 |
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138 GCCUUGUUGGCGCAAUCGGUAGCGCGUAUGACUCUUAAUCAUAAGGUUAGGGGUUCGAGC |
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139 |
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140 ..((((...((((........))))....(((((((((((....))))))))))))))). (-15.59 = -15.53 + -0.06) |
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141 |
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142 |
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143 |
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144 |
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145 |
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146 |
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147 ]]> |
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148 |
0 | 149 </help> |
1
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150 |
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151 <citations> |
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152 <citation type="doi">10.1142/9789814295291_0009</citation> |
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153 </citations> |
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154 |
0 | 155 </tool> |