sailfish: sailfish.xml annotate

annotate sailfish.xml @ 4:03c74355227f draft

planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/sailfish commit edee3afc7d729be9a224d21729bb7490a3761a0e

author	bgruening
date	Sun, 18 Sep 2016 06:05:57 -0400
parents	20eab032389f
children	1b4ed566a41c

rev	line source
4 03c74355227f planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/sailfish commit edee3afc7d729be9a224d21729bb7490a3761a0e bgruening parents: 2 diff changeset	1 <tool id="sailfish" name="Sailfish" version="0.7.6.1">
0 3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	2 <description>transcript quantification from RNA-seq data</description>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	3 <requirements>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	4 <requirement type="package" version="0.7.6">sailfish</requirement>
4 03c74355227f planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/sailfish commit edee3afc7d729be9a224d21729bb7490a3761a0e bgruening parents: 2 diff changeset	5 <requirement type="package" version="1.57.0">boost</requirement>
0 3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	6 </requirements>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	7 <macros>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	8 <xml name="strandedness">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	9 <param name="strandedness" type="select" label="Specify the strandedness of the reads">
1 06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	10 <option value="U" selected="True">Not stranded (U)</option>
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	11 <option value="SF">read 1 (or single-end read) comes from the forward strand (SF)</option>
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	12 <option value="SR">read 1 (or single-end read) comes from the reverse strand (SR)</option>
0 3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	13 </param>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	14 </xml>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	15 </macros>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	16 <stdio>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	17 <exit_code range="1:" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	18 <exit_code range=":-1" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	19 <regex match="Error:" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	20 <regex match="Exception:" />
1 06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	21 <regex match="Exception :" />
0 3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	22 </stdio>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	23 <version_command>sailfish -version</version_command>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	24 <command>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	25 <![CDATA[
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	26
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	27 #if $refTranscriptSource.TranscriptSource == "history":
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	28 sailfish index
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	29 --transcripts $refTranscriptSource.ownFile
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	30 --kmerSize $refTranscriptSource.kmerSize
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	31 --out ./index_dir
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	32 --threads "\${GALAXY_SLOTS:-4}"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	33 #set $index_path = './index_dir'
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	34 #else:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	35 #set $index_path = $refTranscriptSource.index.fields.path
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	36 #end if
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	37
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	38 &&
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	39
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	40 #if $single_or_paired.single_or_paired_opts == 'single':
1 06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	41
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	42 #if $single_or_paired.input_singles.ext == 'fasta':
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	43 #set $ext = 'fasta'
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	44 #else:
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	45 #set $ext = 'fastq'
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	46 #end if
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	47
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	48 ln -s $single_or_paired.input_singles ./single.$ext &&
0 3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	49 #else:
1 06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	50
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	51 #if $single_or_paired.input_mate1.ext == 'fasta':
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	52 #set $ext = 'fasta'
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	53 #else:
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	54 #set $ext = 'fastq'
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	55 #end if
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	56
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	57 ln -s $single_or_paired.input_mate1 ./mate1.$ext &&
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	58 ln -s $single_or_paired.input_mate2 ./mate2.$ext &&
0 3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	59 #end if
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	60
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	61
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	62 #if $geneMap:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	63 ln -s "$geneMap" ./geneMap.$geneMap.ext &&
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	64 #end if
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	65
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	66 sailfish quant
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	67 --index $index_path
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	68 #if $single_or_paired.single_or_paired_opts == 'single':
1 06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	69 --libType ${single_or_paired.strandedness}
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	70 --unmatedReads ./single.$ext
0 3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	71 #else:
1 06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	72 --mates1 ./mate1.$ext
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	73 --mates2 ./mate2.$ext
0 3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	74 --libType "${single_or_paired.orientation}${single_or_paired.strandedness}"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	75 #end if
4 03c74355227f planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/sailfish commit edee3afc7d729be9a224d21729bb7490a3761a0e bgruening parents: 2 diff changeset	76 --output ./
0 3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	77 $biasCorrect
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	78 --threads "\${GALAXY_SLOTS:-4}"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	79
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	80 #if $fldMean:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	81 --fldMean $fldMean
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	82 #end if
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	83
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	84 #if $fldSD:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	85 --fldSD $fldSD
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	86 #end if
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	87
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	88 #if $maxReadOcc:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	89 --maxReadOcc $maxReadOcc
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	90 #end if
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	91
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	92 #if $geneMap:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	93 --geneMap ./geneMap.${geneMap.ext}
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	94 #end if
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	95
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	96 $noEffectiveLengthCorrection
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	97 $useVBOpt
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	98 $allowOrphans
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	99
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	100 $unsmoothedFLD
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	101 --maxFragLen ${maxFragLen}
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	102 --txpAggregationKey "${txpAggregationKey}"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	103
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	104 ]]>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	105 </command>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	106 <inputs>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	107 <conditional name="refTranscriptSource">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	108 <param name="TranscriptSource" type="select" label="Select a reference transcriptome from your history or use a built-in index?" help="Built-ins were indexed using default options">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	109 <option value="indexed">Use a built-in index</option>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	110 <option value="history" selected="True">Use one from the history</option>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	111 </param>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	112 <when value="indexed">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	113 <param name="index" type="select" label="Select a reference transcriptome" help="If your transcriptome of interest is not listed, contact your Galaxy admin">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	114 <options from_data_table="sailfish_indexes">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	115 <filter type="sort_by" column="2"/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	116 <validator type="no_options" message="No indexes are available for the selected input dataset"/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	117 </options>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	118 </param>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	119 </when> <!-- build-in -->
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	120 <when value="history">
4 03c74355227f planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/sailfish commit edee3afc7d729be9a224d21729bb7490a3761a0e bgruening parents: 2 diff changeset	121 <param name="ownFile" type="data" format="fasta" metadata_name="dbkey" label="Select the reference transcriptome" help="in FASTA format" />
0 3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	122 <param argument="kmerSize" type="integer" value="21" max="32" label="The size of the k-mer on which the index is built"
4 03c74355227f planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/sailfish commit edee3afc7d729be9a224d21729bb7490a3761a0e bgruening parents: 2 diff changeset	123 help="There is a tradeoff here between the distinctiveness of the k-mers and their robustness to errors.
0 3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	124 The shorter the k-mers, the more robust they will be to errors in the reads, but the longer the k-mers,
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	125 the more distinct they will be. We generally recommend using a k-mer size of at least 20."/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	126 </when> <!-- history -->
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	127 </conditional> <!-- refTranscriptSource -->
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	128
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	129 <conditional name="single_or_paired">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	130 <param name="single_or_paired_opts" type="select" label="Is this library mate-paired?">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	131 <option value="single">Single-end</option>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	132 <option value="paired">Paired-end</option>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	133 </param>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	134 <when value="single">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	135 <param name="input_singles" type="data" format="fastq,fasta" label="FASTQ/FASTA file" help="FASTQ file." />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	136 <expand macro="strandedness" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	137 </when>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	138 <when value="paired">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	139 <param name="input_mate1" type="data" format="fastq,fasta" label="Mate pair 1" help="FASTQ file." />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	140 <param name="input_mate2" type="data" format="fastq,fasta" label="Mate pair 2" help="FASTQ file." />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	141 <param name="orientation" type="select" label="Relative orientation of reads within a pair">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	142 <option value="M">Mates are oriented in the same direction (M = matching)</option>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	143 <option value="O">Mates are oriented away from each other (O = outward)</option>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	144 <option value="I" selected="True">Mates are oriented toward each other (I = inward)</option>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	145 </param>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	146 <expand macro="strandedness" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	147 </when>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	148 </conditional>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	149
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	150 <param argument="--geneMap" type="data" format="tabular,gff,gtf" optional="True" label="File containing a mapping of transcripts to genes"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	151 help="Calculates the aggregated gene-level abundance estimations. This file should be eiher a GTF file or tab-delimited format
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	152 where each line contains the name of a transcript and the gene to which it belongs separated by a tab." />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	153
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	154 <param argument="--biasCorrect" type="boolean" truevalue="--biasCorrect" falsevalue="" checked="False"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	155 label="Perform bias correction" help=""/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	156
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	157 <param argument="--fldMean" type="integer" value="200" optional="True" label="Calculate effective lengths"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	158 help="If single end reads are being used for quantification, or there are an insufficient number of uniquely mapping reads when performing paired-end quantification
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	159 to estimate the empirical fragment length distribution, then use this value to calculate effective lengths."/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	160
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	161 <param argument="--fldSD" type="integer" value="80" optional="True" label="Standard deviation"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	162 help="The standard deviation used in the fragment length distribution for single-end quantification or when an empirical distribution cannot be learned."/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	163
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	164 <param argument="--maxReadOcc" type="integer" value="200" optional="True" label="Maximal read mapping occurence"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	165 help="Reads mapping to more than this many places won't be considered."/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	166
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	167 <param argument="--noEffectiveLengthCorrection" type="boolean" truevalue="--noEffectiveLengthCorrection" falsevalue="" checked="False"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	168 label="Disable effective length correction" help="Disables effective length correction when computing the probability that a fragment was generated from a transcript.
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	169 If this flag is passed in, the fragment length distribution is not taken into account when computing this probability."/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	170
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	171 <param argument="--useVBOpt" type="boolean" truevalue="--useVBOpt" falsevalue="" checked="False"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	172 label="Use Variational Bayesian EM algorithm for optimization" help=""/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	173
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	174 <param argument="--allowOrphans" type="boolean" truevalue="--allowOrphans" falsevalue="" checked="False"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	175 label="Consider orphaned reads as valid hits when performing lightweight-alignment"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	176 help="This option will increase sensitivity (allow more reads to map and more transcripts to be detected), but may decrease specificity as orphaned alignments are more likely to be spurious."/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	177
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	178 <param argument="--unsmoothedFLD" type="boolean" truevalue="--unsmoothedFLD" falsevalue="" checked="False"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	179 label="Use the un-smoothed approach to effective length correction" help="This traditional approach works by convolving the FLD with the characteristic function over each transcript."/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	180
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	181 <param argument="--maxFragLen" type="integer" value="1000" optional="True"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	182 label="The maximum length of a fragment to consider when building the empirical fragment length distribution"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	183 help=""/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	184
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	185 <param argument="--txpAggregationKey" value="gene_id" type="text" label="The key for aggregating transcripts during gene-level estimates"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	186 help="The default is the gene_id field, but other fields (e.g. gene_name) might be useful depending on the specifics of the annotation being used." />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	187
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	188 </inputs>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	189 <outputs>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	190 <data name="output_quant" format="tabular" from_work_dir="quant.sf" label="${tool.name} on ${on_string} (Quantification)" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	191 <data name="output_bias_corrected_quant" format="tabular" from_work_dir="quant_bias_corrected.sf" label="${tool.name} on ${on_string} (Bias corrected Quantification)">
1 06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	192 <filter>biasCorrect is True</filter>
0 3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	193 </data>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	194 <data name="output_gene_quant" format="tabular" from_work_dir="quant.genes.sf" label="${tool.name} on ${on_string} (Gene Quantification)">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	195 <filter>geneMap is True</filter>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	196 </data>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	197 </outputs>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	198 <tests>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	199 <test>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	200 <param name="single_or_paired_opts" value="paired" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	201 <param name="input_mate1" value="reads_1.fastq" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	202 <param name="input_mate2" value="reads_2.fastq" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	203 <param name="biasCorrect" value="True" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	204 <param name="TranscriptSource" value="history" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	205 <param name="ownFile" value="transcripts.fasta" ftype="fasta" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	206 <output file="sailfish_quant_result1.tab" ftype="tabular" name="output_quant" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	207 <output file="sailfish_bias_result1.tab" ftype="tabular" name="output_bias_corrected_quant" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	208 </test>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	209 </tests>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	210 <help>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	211 <![CDATA[
4 03c74355227f planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/sailfish commit edee3afc7d729be9a224d21729bb7490a3761a0e bgruening parents: 2 diff changeset	212
0 3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	213 What it does
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	214
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	215 Sailfish is a tool for transcript quantification from RNA-seq data. It
2 20eab032389f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit b9ccc98180d52233931768511b909ccd6142f158 bgruening parents: 1 diff changeset	216 requires a set of target transcripts (either from a reference or de-novo
0 3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	217 assembly) to quantify. All you need to run Sailfish is a fasta file containing
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	218 your reference transcripts and a (set of) fasta/fastq file(s) containing your
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	219 reads. Sailfish runs in two phases; indexing and quantification. The indexing
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	220 step is independent of the reads, and only need to be run one for a particular
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	221 set of reference transcripts and choice of k (the k-mer size). The
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	222 quantification step, obviously, is specific to the set of RNA-seq reads and is
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	223 thus run more frequently.
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	224
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	225 When the quantification output contains a number of columns:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	226 (1) Transcript ID,
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	227 (2) Transcript Length,
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	228 (3) Transcripts per Million (TPM) and
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	229 (4) Estimated number of reads (an estimate of the number of reads drawn from this transcript given the transcript’s relative abundance and length).
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	230
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	231 The first two columns are self-explanatory, the next four are measures of transcript abundance and the final is a commonly used input for differential expression tools.
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	232 The Transcripts per Million quantification number is computed as described in [1], and is meant as an estimate of the number of transcripts, per million observed transcripts,
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	233 originating from each isoform. Its benefit over the F/RPKM measure is that it is independent of the mean expressed transcript length
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	234 (i.e. if the mean expressed transcript length varies between samples, for example, this alone can affect differential analysis based on the K/RPKM.).
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	235
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	236
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	237
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	238 Fragment Library Types
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	239 ======================
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	240
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	241 There are numerous library preparation protocols for RNA-seq that result in
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	242 sequencing reads with different characteristics. For example, reads can be
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	243 single end (only one side of a fragment is recorded as a read) or paired-end
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	244 (reads are generated from both ends of a fragment). Further, the sequencing
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	245 reads themselves may be unstraned or strand-specific. Finally, paired-end
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	246 protocols will have a specified relative orientation. To characterize the
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	247 various different typs of sequencing libraries, we've created a miniature
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	248 "language" that allows for the succinct description of the many different types
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	249 of possible fragment libraries. For paired-end reads, the possible
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	250 orientations, along with a graphical description of what they mean, are
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	251 illustrated below:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	252
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	253 .. image:: ReadLibraryIllustration.png
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	254
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	255 The library type string consists of three parts: the relative orientation of
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	256 the reads, the strandedness of the library, and the directionality of the
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	257 reads.
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	258
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	259 The first part of the library string (relative orientation) is only provided if
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	260 the library is paired-end. The possible options are:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	261
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	262 ::
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	263
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	264 I = inward
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	265 O = outward
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	266 M = matching
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	267
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	268 The second part of the read library string specifies whether the protocol is
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	269 stranded or unstranded; the options are:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	270
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	271 ::
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	272
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	273 S = stranded
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	274 U = unstranded
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	275
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	276 If the protocol is unstranded, then we're done. The final part of the library
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	277 string specifies the strand from which the read originates in a strand-specific
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	278 protocol — it is only provided if the library is stranded (i.e. if the
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	279 library format string is of the form S). The possible values are:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	280
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	281 ::
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	282
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	283 F = read 1 (or single-end read) comes from the forward strand
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	284 R = read 1 (or single-end read) comes from the reverse strand
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	285
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	286 So, for example, if you wanted to specify a fragment library of strand-specific
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	287 paired-end reads, oriented toward each other, where read 1 comes from the
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	288 forward strand and read 2 comes from the reverse strand, you would specify ``-l
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	289 ISF`` on the command line. This designates that the library being processed has
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	290 the type "ISF" meaning, I\ nward (the relative orientation), S\ tranted
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	291 (the protocol is strand-specific), F\ orward (read 1 comes from the forward
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	292 strand).
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	293
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	294 The single end library strings are a bit simpler than their pair-end counter
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	295 parts, since there is no relative orientation of which to speak. Thus, the
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	296 only possible library format types for single-end reads are ``U`` (for
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	297 unstranded), ``SF`` (for strand-specific reads coming from the forward strand)
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	298 and ``SR`` (for strand-specific reads coming from the reverse strand).
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	299
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	300 A few more examples of some library format strings and their interpretations are:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	301
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	302 ::
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	303
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	304 IU (an unstranded paired-end library where the reads face each other)
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	305
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	306 ::
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	307
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	308 SF (a stranded single-end protocol where the reads come from the forward strand)
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	309
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	310 ::
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	311
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	312 OSR (a stranded paired-end protocol where the reads face away from each other,
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	313 read1 comes from reverse strand and read2 comes from the forward strand)
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	314
4 03c74355227f planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/sailfish commit edee3afc7d729be9a224d21729bb7490a3761a0e bgruening parents: 2 diff changeset	315 .. note:: Correspondence to TopHat library types
0 3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	316
4 03c74355227f planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/sailfish commit edee3afc7d729be9a224d21729bb7490a3761a0e bgruening parents: 2 diff changeset	317 The popular `TopHat <http://ccb.jhu.edu/software/tophat/index.shtml>`_ RNA-seq
0 3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	318 read aligner has a different convention for specifying the format of the library.
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	319 Below is a table that provides the corresponding sailfish/salmon library format
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	320 string for each of the potential TopHat library types:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	321
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	322
4 03c74355227f planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/sailfish commit edee3afc7d729be9a224d21729bb7490a3761a0e bgruening parents: 2 diff changeset	323 +---------------------+-------------------------+
0 3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	324 \| TopHat \| Salmon (and Sailfish) \|
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	325 +=====================+============+============+
4 03c74355227f planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/sailfish commit edee3afc7d729be9a224d21729bb7490a3761a0e bgruening parents: 2 diff changeset	326 \| \| Paired-end \| Single-end \|
0 3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	327 +---------------------+------------+------------+
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	328 \|``-fr-unstranded`` \|``-l IU`` \|``-l U`` \|
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	329 +---------------------+------------+------------+
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	330 \|``-fr-firststrand`` \|``-l ISR`` \|``-l SR`` \|
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	331 +---------------------+------------+------------+
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	332 \|``-fr-secondstrand`` \|``-l ISF`` \|``-l SF`` \|
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	333 +---------------------+------------+------------+
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	334
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	335 The remaining salmon library format strings are not directly expressible in terms
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	336 of the TopHat library types, and so there is no direct mapping for them.
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	337
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	338
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	339 ]]>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	340 </help>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	341 </tool>

Mercurial > repos > bgruening > sailfish

annotate sailfish.xml @ 4:03c74355227f draft