annotate fastx_trimmer.xml @ 5:547e8d00f11c draft

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author iuc
date Wed, 22 Aug 2018 11:06:02 -0400
parents b98f3fa516a3
children 61b4cedc8934
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1 <tool id="cshl_fastx_trimmer" version="1.0.2" name="Trim sequences">
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2 <description></description>
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
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6 <expand macro="requirements" />
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7 <command detect_errors="exit_code"><![CDATA[
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8 @CATS@ fastx_trimmer -v
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9 -f $first
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10 -l $last
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11 -o '$output'
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12 @FQQUAL@
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13 @GZIP@
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14 ]]></command>
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15 <inputs>
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16 <expand macro="fastx_input" />
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17 <param name="first" type="integer" value="1" label="First base to keep" />
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18 <param name="last" type="integer" value="21" label="Last base to keep" />
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19 </inputs>
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20 <outputs>
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21 <data name="output" format_source="input" metadata_source="input" />
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22 </outputs>
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23 <tests>
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24 <test>
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25 <!-- Trim a FASTA file - remove first four bases (e.g. a barcode) -->
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26 <param name="input" value="fastx_trimmer1.fasta" />
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27 <param name="first" value="5"/>
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28 <param name="last" value="36"/>
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29 <output name="output" ftype="fasta" file="fastx_trimmer1.out" />
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30 </test>
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31 <test>
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32 <!-- Trim a FASTQ file - remove last 9 bases (e.g. keep only miRNA length sequences) -->
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33 <param name="input" value="fastx_trimmer2.fastq" ftype="fastqsolexa"/>
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34 <param name="first" value="1"/>
377ac2829eac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/fastx_toolkit/fastx_trimmer commit a1517c9d22029095120643bbe2c8fa53754dd2b7
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35 <param name="last" value="27"/>
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36 <output name="output" ftype="fastqsolexa" file="fastx_trimmer2.out" />
377ac2829eac planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/fastx_toolkit/fastx_trimmer commit a1517c9d22029095120643bbe2c8fa53754dd2b7
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37 </test>
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38 <test>
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39 <!-- Trim a FASTQ.gz file and output FASTQ.gz -->
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40 <param name="input" value="fastx_trimmer2.fastq.gz" ftype="fastqsanger.gz"/>
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41 <param name="first" value="1"/>
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42 <param name="last" value="27"/>
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43 <output name="output" ftype="fastqsanger.gz" decompress="true" file="fastx_trimmer2.out.gz" />
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44 </test>
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45 </tests>
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46 <help><![CDATA[
0
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47 **What it does**
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48
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49 This tool trims (cut bases from) sequences in a FASTA/Q file.
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50
0
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51 --------
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52
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53 **Example**
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54
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55 Input Fasta file (with 36 bases in each sequences)::
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56
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57 >1-1
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58 TATGGTCAGAAACCATATGCAGAGCCTGTAGGCACC
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59 >2-1
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60 CAGCGAGGCTTTAATGCCATTTGGCTGTAGGCACCA
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61
0
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62 Trimming with First=1 and Last=21, we get a FASTA file with 21 bases in each sequences (starting from the first base)::
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63
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64 >1-1
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65 TATGGTCAGAAACCATATGCA
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66 >2-1
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67 CAGCGAGGCTTTAATGCCATT
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68
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69 Trimming with First=6 and Last=10, will generate a FASTA file with 5 bases (bases 6,7,8,9,10) in each sequences::
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70
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71 >1-1
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72 TCAGA
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73 >2-1
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74 AGGCT
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75
0
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76 ------
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77
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78 This tool is based on `FASTX-toolkit`__ by Assaf Gordon.
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79
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80 .. __: http://hannonlab.cshl.edu/fastx_toolkit/
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81 ]]></help>
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82 <expand macro="citations" />
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83 <!-- FASTX-Trimmer is part of the FASTX-toolkit, by A.Gordon (gordon@cshl.edu) -->
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84 </tool>