Mercurial > repos > drosofff > blastx_to_scaffold
annotate blastx_to_scaffold.xml @ 1:940c0c669e96 draft default tip
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author | drosofff |
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date | Wed, 09 Nov 2016 11:39:20 -0500 |
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1 <tool id="blastx2scaffold" name="blastx_to_scaffold" version="0.9.0"> |
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2 <description>Generate DNA scaffold from blastx alignment of Contigs</description> |
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3 <requirements> |
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4 </requirements> |
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5 <command interpreter="python"> |
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6 blastx_to_scaffold.py --sequences $sequences |
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7 --blastx-tab $blastx_tab |
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8 --output $output |
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9 </command> |
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10 <inputs> |
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11 <param name="sequences" type="data" format="fasta" label="Select a fasta contigs file"/> |
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12 <param name="blastx_tab" type="data" format="tabular" label="Select a blastx output from your history" help="must have 13 columns with column 13 containing the subject lenght, other columns are standard"/> |
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13 |
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14 </inputs> |
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15 <outputs> |
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16 <data format="fasta" name="output"/> |
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17 </outputs> |
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18 |
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19 |
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20 <tests> |
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21 <test> |
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22 <param name="sequences" value="contigs.fa" ftype="fasta"/> |
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23 <param name="blastx_tab" value="blastx.tab" ftype="tabular"/> |
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24 <output name="output" file="scaffold.fa" ftype="fasta"/> |
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25 </test> |
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26 </tests> |
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27 |
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28 |
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29 <help> |
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30 |
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31 |
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32 **What it Does** |
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33 This tool starts from DNA contigs that aligned to a subject protein sequence through blastx. |
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34 The contigs must be provided in fasta format. The blastx output must be tabular, the 12 standard column plus column 13 with the length of the blastx subject. |
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35 The final scaffold is a DNA sequence. |
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36 Sequences of the subject protein which were not aligned to the contigs are replaced by Ns in this scaffold. |
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37 |
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38 **Attribution** |
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39 This Galaxy tool was created by drosofff@gmail.com on 28/05/2015 |
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40 </help> |
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41 |
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42 </tool> |