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changeset 2:1b6570fe022e draft

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from test toolshed - bumped version
author fubar
date Sun, 01 Dec 2013 20:02:56 -0500
parents cf59a9ae0efe
children 9c4d943fbef7
files README clustalw-76de6971a0de/rgclustal/README clustalw-76de6971a0de/rgclustal/rgClustalw.xml clustalw-76de6971a0de/rgclustal/test-data/rgClustal_testin.fasta clustalw-76de6971a0de/rgclustal/test-data/rgClustal_testout.fasta clustalw-76de6971a0de/rgclustal/test-data/rgClustal_testout.log clustalw-76de6971a0de/rgclustal/tool_dependencies.xml rgClustalw.xml test-data/rgClustal_testin.fasta test-data/rgClustal_testout.fasta test-data/rgClustal_testout.log tool_dependencies.xml
diffstat 12 files changed, 387 insertions(+), 387 deletions(-) [+]
line wrap: on
line diff
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/README	Sun Dec 01 20:02:56 2013 -0500
@@ -0,0 +1,55 @@
+Updated july 11 2013 for automated toolshed installation
+
+** This is a wrapper for ClustalW **
+
+This tool allows you to align multiple sequences in Galaxy, using ClustalW2_ with mostly default options which should work reasonably well for many alignments.
+DNA or protein sequences can be aligned. The input file must be a fasta file in your current history.
+
+The alignments will appear as a clustal format file or optionally, as phylip or fasta format files in your history and a text log will be output to your history 
+showing the output Clustalw would normally write to standard output.
+
+If Clustal format is chosen, you have the option of adding basepair counts to the output
+
+A subsequence of the alignment can be output by setting the Output complete parameter to "Partial" and defining the offset and end of the subsequence to be output 
+
+**Installation**
+
+As of July 2013, automated installation from the tool shed should have worked.
+
+Otherwise, the old skool way was:
+
+Make sure clustalw2 is available on the path for all your nodes
+
+Move the test data files to your galaxy root test-data
+Move the xml file to a subdirectory of your tools folder (eg rgenetics/) and then add a line in your tool_conf.xml to point there.
+Run
+sh run_functional_tests.sh -id clustalw
+to make sure the tests work
+
+then restart Galaxy and you should be good to go.
+
+**Attribution**
+
+Clustal attribution and associated documentation are available at http://www.clustal.org
+
+An implementation of a Galaxy Clustal wrapper was written by Hans-Rudolf Hotz in an email on the developer list - 
+http://lists.bx.psu.edu/pipermail/galaxy-dev/2010-November/003732.html
+
+This version by Ross Lazarus for the rgenetics project, builds on Hans-Rudolf's code, adding some additional controls and a log file. It also
+deals with stderr so Cluastalw2 writing there doesn't cause the job to error out. That's encoded in the tail of the command line. 
+
+**License**
+
+Assuming Hans-Rudolf is ok with a new license for this derived work, this version of his wrapper is LGPL like other rgenetics artefacts
+
+Written by Ross Lazarus for the Rgenetics project
+
+Copyright Ross Lazarus at gmail com 2011
+
+All rights reserved.
+
+Released under the LGPL - see http://www.gnu.org/copyleft/lesser.html
+
+
+
+
--- a/clustalw-76de6971a0de/rgclustal/README	Sun Dec 01 19:57:03 2013 -0500
+++ /dev/null	Thu Jan 01 00:00:00 1970 +0000
@@ -1,55 +0,0 @@
-Updated july 11 2013 for automated toolshed installation
-
-** This is a wrapper for ClustalW **
-
-This tool allows you to align multiple sequences in Galaxy, using ClustalW2_ with mostly default options which should work reasonably well for many alignments.
-DNA or protein sequences can be aligned. The input file must be a fasta file in your current history.
-
-The alignments will appear as a clustal format file or optionally, as phylip or fasta format files in your history and a text log will be output to your history 
-showing the output Clustalw would normally write to standard output.
-
-If Clustal format is chosen, you have the option of adding basepair counts to the output
-
-A subsequence of the alignment can be output by setting the Output complete parameter to "Partial" and defining the offset and end of the subsequence to be output 
-
-**Installation**
-
-As of July 2013, automated installation from the tool shed should have worked.
-
-Otherwise, the old skool way was:
-
-Make sure clustalw2 is available on the path for all your nodes
-
-Move the test data files to your galaxy root test-data
-Move the xml file to a subdirectory of your tools folder (eg rgenetics/) and then add a line in your tool_conf.xml to point there.
-Run
-sh run_functional_tests.sh -id clustalw
-to make sure the tests work
-
-then restart Galaxy and you should be good to go.
-
-**Attribution**
-
-Clustal attribution and associated documentation are available at http://www.clustal.org
-
-An implementation of a Galaxy Clustal wrapper was written by Hans-Rudolf Hotz in an email on the developer list - 
-http://lists.bx.psu.edu/pipermail/galaxy-dev/2010-November/003732.html
-
-This version by Ross Lazarus for the rgenetics project, builds on Hans-Rudolf's code, adding some additional controls and a log file. It also
-deals with stderr so Cluastalw2 writing there doesn't cause the job to error out. That's encoded in the tail of the command line. 
-
-**License**
-
-Assuming Hans-Rudolf is ok with a new license for this derived work, this version of his wrapper is LGPL like other rgenetics artefacts
-
-Written by Ross Lazarus for the Rgenetics project
-
-Copyright Ross Lazarus at gmail com 2011
-
-All rights reserved.
-
-Released under the LGPL - see http://www.gnu.org/copyleft/lesser.html
-
-
-
-
--- a/clustalw-76de6971a0de/rgclustal/rgClustalw.xml	Sun Dec 01 19:57:03 2013 -0500
+++ /dev/null	Thu Jan 01 00:00:00 1970 +0000
@@ -1,128 +0,0 @@
-<tool id="clustalw" name="ClustalW" version="0.1">
-   <description>multiple sequence alignment program for DNA or proteins</description>
-   <requirements>
-      <requirement type="package" version="2.1">clustalw</requirement>
-  </requirements>
-   <command> 
-    #if ($range.mode=="part")
-    clustalw2 -infile=$input -outfile=$output -OUTORDER=$out_order -RANGE=$range.seq_range_start,$range.seq_range_end
-    #elif ($range.mode=="complete")
-    clustalw2 -infile=$input -outfile=$output -OUTORDER=$out_order 
-    #end if
-    #if ($outcontrol.outform=="clustal")
-    -SEQNOS=$outcontrol.out_seqnos
-    #end if
-    #if ($outcontrol.outform=="phylip")
-    -OUTPUT=PHYLIP
-    #end if
-    #if ($outcontrol.outform=="fasta")
-    -OUTPUT=FASTA
-    #end if
-    -TYPE=$dnarna 1&gt;$outlog  2&gt;&amp;1
-   </command>
-  <inputs>
-   <page>
-    <param format="fasta" name="input" type="data" label="Fasta File" />
-    <param name="outname" label="Name for output files to make it easy to remember what you did" type="text" size="50" value="Clustal_run" />
-    <param name="dnarna" type="select" label="Data Type">
-      <option value="DNA" selected="True">DNA nucleotide sequences</option>
-      <option value="PROTEIN">Protein sequences</option>
-    </param>
-    <conditional name="outcontrol">
-      <param name="outform" type="select" label="Output alignment format">
-        <option value="clustal" selected="True">Native Clustal output format</option>
-        <option value="phylip">Phylip format</option>
-        <option value="fasta">Fasta format</option>
-      </param>
-      <when value="fasta" />
-      <when value="phylip" />
-      <when value="clustal">
-       <param name="out_seqnos" type="select" label="Show residue numbers in clustal format output">
-         <option value="ON">yes</option>
-         <option value="OFF" selected="true">no</option>
-       </param>
-      </when>
-    </conditional>
-    <param name="out_order" type="select" label="Output Order">
-      <option value="ALIGNED">aligned</option>
-      <option value="INPUT">same order as input file</option>
-    </param>
-
-    <conditional name="range">
-        <param name="mode" type="select" label="Output complete alignment (or specify part to output)">
-          <option value="complete">complete alignment</option>
-          <option value="part">only part of the alignment</option>
-        </param>
-        <when value="complete">
-        </when>
-        <when value="part">    
-           <param name="seq_range_start" size="5" type="integer" value="1" label="start point" help="sequence range to write">
-           </param>
-           <param name="seq_range_end" size="5" type="integer" value="99999" label="end point" >
-           </param> 
-        </when>
-    </conditional>
-   </page>
-  </inputs>
-  <outputs>
-    <data format="clustal" name="output"  label="${outname}_output.${outcontrol.outform}">
-       <change_format>
-           <when input="outcontrol.outform" value="phylip" format="phylip" />
-           <when input="outcontrol.outform" value="fasta" format="fasta" />
-       </change_format>
-    </data>
-    <data format="txt" name="outlog"  label="${outname}_clustal_log.txt"/>
-  </outputs>
-  <tests>
-     <test>
-        <param name="input" value="rgClustal_testin.fasta" />
-      <param name = "outname" value="" />
-      <param name = "outform" value="fasta" />
-      <param name = "dnarna" value="DNA" />
-      <param name = "mode" value="complete" />
-      <param name = "out_order" value="ALIGNED" />
-      <output name="output" file="rgClustal_testout.fasta" ftype="fasta" />
-      <output name="output" file="rgClustal_testout.log" ftype="txt" lines_diff="5" />
-     </test>
-  </tests>
-  <help>
-
-**Note**
-
-This tool allows you to run a multiple sequence alignment with ClustalW2 (see Clustsrc_) using the default options.
- 
-For a tutorial introduction, see ClustalW2_
-
-You can align DNA or protein sequences in the input file which should be multiple sequences to be aligned in a fasta file
-
-A log will be output to your history showing the output Clustal would normally write to standard output.
-
-The alignments will appear as a clustal format file or optionally, as phylip or fasta format files in your history. If you choose fasta as 
-the output format, you can create a 'Logo' image using the Sequence Logo tool.
-
-If Clustal format is chosen, you have the option of adding basepair counts to the output
-
-A subsequence of the alignment can be output by setting the Output complete parameter to "Partial" and defining the offset and end of the subsequence to be output 
-
-----
-
-**Attribution**
-
-Clustal attribution and associated documentation are available at Clustsrc_
-
-The first iteration of this Galaxy wrapper was written by Hans-Rudolf Hotz - see Clustfirst_
-
-It was modified by Ross Lazarus for the rgenetics project
-
-This wrapper is now LGPL
-
-.. _ClustalW2: http://www.ebi.ac.uk/2can/tutorials/protein/clustalw.html  
-
-.. _Clustsrc: http://www.clustal.org
-
-.. _Clustfirst: http://lists.bx.psu.edu/pipermail/galaxy-dev/2010-November/003732.html
-
-  </help>
-
-</tool>
-
--- a/clustalw-76de6971a0de/rgclustal/test-data/rgClustal_testin.fasta	Sun Dec 01 19:57:03 2013 -0500
+++ /dev/null	Thu Jan 01 00:00:00 1970 +0000
@@ -1,25 +0,0 @@
->c_briggsae-chrII(+)/43862-46313
-ATGAGCTTCCACAAAAGCATGAGCTTTCTCAGCTTCTGCCACATCAGCATTCAAATGATC
->c_remanei-Crem_Contig172(-)/123228-124941
-ATGAGCCTCTACAACCGCATGATTCTTTTCAGCCTCTGCCACGTCCGCATTCAAATGCTC
->c_brenneri-Cbre_Contig60(+)/627772-630087
-ATGAGCCTCCACAACAGCATGATTTTTCTCGGCTTCCGCCACATCCGCATTCAAATGATC
->c_elegans-II(+)/9706834-9708803
-ATGAGCCTCTACTACAGCATGATTCTTCTCAGCTTCTGCAACGTCAGCATTCAGATGATC
->c_briggsae-chrIfooI(+)/43862-46313
-CGCACAAATATGATGCACAAATCCACAACCTAAAGCATCTCCGATAACGTTGACCGAAGT
->c_remanei-Crem_Contig172foo(-)/123228-124941
-AGCACAAATGTAATGAACGAATCCGCATCCCAACGCATCGCCAATCACATTCACAGATGT
->c_brenneri-Cbre_Contig60gak(+)/627772-630087
-CGCACAAATGTAGTGGACAAATCCGCATCCCAAAGCGTCTCCGATAACATTTACCGAAGT
->c_elegans-II(+)more/9706834-9708803
-TGCACAAATGTGATGAACGAATCCACATCCCAATGCATCACCGATCACATTGACAGATGT
->c_briggsae-chrII(+)bar/43862-46313
-CCGGAGTCGATCCCTGAAT-----------------------------------------
->c_remanei-Crem_Contig172zot(-)/123228-124941
-ACGAAGTCGGTCCCTATAAGGTATGATTTTATATGA----TGTACCATAAGGAAATAGTC
->c_brenneri-Cbre_Contig60fee(+)/627772-630087
-ACGAAGTCGATCCCTGAAA---------TCAGATGAGCGGTTGACCA---GAGAACAACC
->c_elegans-II(+)meh/9706834-9708803
-ACGAAGTCGGTCCCTGAAC--AATTATTT----TGA----TATA---GAAAGAAACGGTA
-
--- a/clustalw-76de6971a0de/rgclustal/test-data/rgClustal_testout.fasta	Sun Dec 01 19:57:03 2013 -0500
+++ /dev/null	Thu Jan 01 00:00:00 1970 +0000
@@ -1,48 +0,0 @@
->c_briggsae-chrII_+_
----ATGAGCTTCCACAAAAGCATGAGCTTT
-CTCAGCTTCTGCCACATCAGCATTCAAATG
-ATC
->c_brenneri-Cbre_Contig60_+_
----ATGAGCCTCCACAACAGCATGATTTTT
-CTCGGCTTCCGCCACATCCGCATTCAAATG
-ATC
->c_remanei-Crem_Contig172_-_
----ATGAGCCTCTACAACCGCATGATTCTT
-TTCAGCCTCTGCCACGTCCGCATTCAAATG
-CTC
->c_elegans-II_+_
----ATGAGCCTCTACTACAGCATGATTCTT
-CTCAGCTTCTGCAACGTCAGCATTCAGATG
-ATC
->c_briggsae-chrII_+_bar
----CCGGAGTCGATCCCTGAAT--------
-------------------------------
----
->c_brenneri-Cbre_Contig60fee_+_
----ACGAAGTCGATCCCTGAAA--------
--TCAGATGAGCGGTTGACCA---GAGAACA
-ACC
->c_remanei-Crem_Contig172zot_-_
----ACGAAGTCGGTCCCTATAAGGTATGAT
-TTTATATGA----TGTACCATAAGGAAATA
-GTC
->c_elegans-II_+_meh
----ACGAAGTCGGTCCCTGAAC--AATTAT
-TT----TGA----TATA---GAAAGAAACG
-GTA
->c_briggsae-chrIfooI_+_
-CGCACAAATATGATGCACAAATCCACAACC
-TAAAGCATCTCCGATAACGTTGACCGAAGT
----
->c_brenneri-Cbre_Contig60gak_+_
-CGCACAAATGTAGTGGACAAATCCGCATCC
-CAAAGCGTCTCCGATAACATTTACCGAAGT
----
->c_remanei-Crem_Contig172foo_-_
-AGCACAAATGTAATGAACGAATCCGCATCC
-CAACGCATCGCCAATCACATTCACAGATGT
----
->c_elegans-II_+_more
-TGCACAAATGTGATGAACGAATCCACATCC
-CAATGCATCACCGATCACATTGACAGATGT
----
--- a/clustalw-76de6971a0de/rgclustal/test-data/rgClustal_testout.log	Sun Dec 01 19:57:03 2013 -0500
+++ /dev/null	Thu Jan 01 00:00:00 1970 +0000
@@ -1,112 +0,0 @@
-
-
-
- CLUSTAL 2.1 Multiple Sequence Alignments
-
-
-Sequence type explicitly set to DNA
-Sequence format is Pearson
-Sequence 1: c_briggsae-chrII_+_/43862-46313                 60 bp
-Sequence 2: c_remanei-Crem_Contig172_-_/123228-124941       60 bp
-Sequence 3: c_brenneri-Cbre_Contig60_+_/627772-630087       60 bp
-Sequence 4: c_elegans-II_+_/9706834-9708803                 60 bp
-Sequence 5: c_briggsae-chrIfooI_+_/43862-46313              60 bp
-Sequence 6: c_remanei-Crem_Contig172foo_-_/123228-124941    60 bp
-Sequence 7: c_brenneri-Cbre_Contig60gak_+_/627772-630087    60 bp
-Sequence 8: c_elegans-II_+_more/9706834-9708803             60 bp
-Sequence 9: c_briggsae-chrII_+_bar/43862-46313              60 bp
-Sequence 10: c_remanei-Crem_Contig172zot_-_/123228-124941    60 bp
-Sequence 11: c_brenneri-Cbre_Contig60fee_+_/627772-630087    60 bp
-Sequence 12: c_elegans-II_+_meh/9706834-9708803              60 bp
-Start of Pairwise alignments
-Aligning...
-
-Sequences (1:2) Aligned. Score:  80
-Sequences (1:3) Aligned. Score:  88
-Sequences (1:4) Aligned. Score:  83
-Sequences (1:5) Aligned. Score:  21
-Sequences (1:6) Aligned. Score:  20
-Sequences (1:7) Aligned. Score:  23
-Sequences (1:8) Aligned. Score:  18
-Sequences (1:9) Aligned. Score:  21
-Sequences (1:10) Aligned. Score:  16
-Sequences (1:11) Aligned. Score:  25
-Sequences (1:12) Aligned. Score:  10
-Sequences (2:3) Aligned. Score:  85
-Sequences (2:4) Aligned. Score:  86
-Sequences (2:5) Aligned. Score:  21
-Sequences (2:6) Aligned. Score:  20
-Sequences (2:7) Aligned. Score:  25
-Sequences (2:8) Aligned. Score:  20
-Sequences (2:9) Aligned. Score:  36
-Sequences (2:10) Aligned. Score:  16
-Sequences (2:11) Aligned. Score:  22
-Sequences (2:12) Aligned. Score:  17
-Sequences (3:4) Aligned. Score:  85
-Sequences (3:5) Aligned. Score:  13
-Sequences (3:6) Aligned. Score:  20
-Sequences (3:7) Aligned. Score:  25
-Sequences (3:8) Aligned. Score:  20
-Sequences (3:9) Aligned. Score:  36
-Sequences (3:10) Aligned. Score:  16
-Sequences (3:11) Aligned. Score:  18
-Sequences (3:12) Aligned. Score:  25
-Sequences (4:5) Aligned. Score:  13
-Sequences (4:6) Aligned. Score:  11
-Sequences (4:7) Aligned. Score:  20
-Sequences (4:8) Aligned. Score:  10
-Sequences (4:9) Aligned. Score:  31
-Sequences (4:10) Aligned. Score:  17
-Sequences (4:11) Aligned. Score:  29
-Sequences (4:12) Aligned. Score:  14
-Sequences (5:6) Aligned. Score:  73
-Sequences (5:7) Aligned. Score:  83
-Sequences (5:8) Aligned. Score:  80
-Sequences (5:9) Aligned. Score:  31
-Sequences (5:10) Aligned. Score:  14
-Sequences (5:11) Aligned. Score:  14
-Sequences (5:12) Aligned. Score:  12
-Sequences (6:7) Aligned. Score:  80
-Sequences (6:8) Aligned. Score:  88
-Sequences (6:9) Aligned. Score:  26
-Sequences (6:10) Aligned. Score:  16
-Sequences (6:11) Aligned. Score:  25
-Sequences (6:12) Aligned. Score:  12
-Sequences (7:8) Aligned. Score:  78
-Sequences (7:9) Aligned. Score:  31
-Sequences (7:10) Aligned. Score:  10
-Sequences (7:11) Aligned. Score:  12
-Sequences (7:12) Aligned. Score:  12
-Sequences (8:9) Aligned. Score:  31
-Sequences (8:10) Aligned. Score:  10
-Sequences (8:11) Aligned. Score:  14
-Sequences (8:12) Aligned. Score:  12
-Sequences (9:10) Aligned. Score:  63
-Sequences (9:11) Aligned. Score:  84
-Sequences (9:12) Aligned. Score:  78
-Sequences (10:11) Aligned. Score:  64
-Sequences (10:12) Aligned. Score:  76
-Sequences (11:12) Aligned. Score:  46
-Guide tree file created:   [/share/shared/galaxy/database/files/002/dataset_2705.dnd]
-
-There are 11 groups
-Start of Multiple Alignment
-
-Aligning...
-Group 1: Sequences:   2      Score:1045
-Group 2: Sequences:   2      Score:1016
-Group 3: Sequences:   4      Score:1001
-Group 4: Sequences:   2      Score:313
-Group 5: Sequences:   2      Score:731
-Group 6: Sequences:   4      Score:516
-Group 7: Sequences:   8      Score:344
-Group 8: Sequences:   2      Score:1016
-Group 9: Sequences:   2      Score:1054
-Group 10: Sequences:   4      Score:945
-Group 11: Sequences:  12      Score:380
-Alignment Score 6283
-firstres = 1 lastres = 63
-FASTA file created!
-
-Fasta-Alignment file created    [/share/shared/galaxy/database/files/002/dataset_2726.dat]
-
--- a/clustalw-76de6971a0de/rgclustal/tool_dependencies.xml	Sun Dec 01 19:57:03 2013 -0500
+++ /dev/null	Thu Jan 01 00:00:00 1970 +0000
@@ -1,19 +0,0 @@
-<?xml version="1.0" encoding="UTF-8"?>
-<tool_dependency>
-    <package name="clustalw" version="2.1">
-        <install version="1.0"> 
-           <actions>
-                <action type="download_by_url">http://www.clustal.org/download/current/clustalw-2.1.tar.gz</action>
-                <action type="shell_command">./configure &amp;&amp; make</action>
-                <action type="move_file"><source>src/clustalw2</source><destination>$INSTALL_DIR</destination></action>
-                <action type="set_environment">
-                    <environment_variable action="prepend_to" name="PATH">$INSTALL_DIR</environment_variable>
-                </action>
-           </actions>
-        </install>
-        <readme>Installs clustalw from http://www.clustal.org/download/current/clustalw-2.1.tar.gz
-       </readme>
-    </package>
-</tool_dependency>
-
-
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/rgClustalw.xml	Sun Dec 01 20:02:56 2013 -0500
@@ -0,0 +1,128 @@
+<tool id="clustalw" name="ClustalW" version="0.2">
+   <description>multiple sequence alignment program for DNA or proteins</description>
+   <requirements>
+      <requirement type="package" version="2.1">clustalw</requirement>
+  </requirements>
+   <command> 
+    #if ($range.mode=="part")
+    clustalw2 -infile=$input -outfile=$output -OUTORDER=$out_order -RANGE=$range.seq_range_start,$range.seq_range_end
+    #elif ($range.mode=="complete")
+    clustalw2 -infile=$input -outfile=$output -OUTORDER=$out_order 
+    #end if
+    #if ($outcontrol.outform=="clustal")
+    -SEQNOS=$outcontrol.out_seqnos
+    #end if
+    #if ($outcontrol.outform=="phylip")
+    -OUTPUT=PHYLIP
+    #end if
+    #if ($outcontrol.outform=="fasta")
+    -OUTPUT=FASTA
+    #end if
+    -TYPE=$dnarna 1&gt;$outlog  2&gt;&amp;1
+   </command>
+  <inputs>
+   <page>
+    <param format="fasta" name="input" type="data" label="Fasta File" />
+    <param name="outname" label="Name for output files to make it easy to remember what you did" type="text" size="50" value="Clustal_run" />
+    <param name="dnarna" type="select" label="Data Type">
+      <option value="DNA" selected="True">DNA nucleotide sequences</option>
+      <option value="PROTEIN">Protein sequences</option>
+    </param>
+    <conditional name="outcontrol">
+      <param name="outform" type="select" label="Output alignment format">
+        <option value="clustal" selected="True">Native Clustal output format</option>
+        <option value="phylip">Phylip format</option>
+        <option value="fasta">Fasta format</option>
+      </param>
+      <when value="fasta" />
+      <when value="phylip" />
+      <when value="clustal">
+       <param name="out_seqnos" type="select" label="Show residue numbers in clustal format output">
+         <option value="ON">yes</option>
+         <option value="OFF" selected="true">no</option>
+       </param>
+      </when>
+    </conditional>
+    <param name="out_order" type="select" label="Output Order">
+      <option value="ALIGNED">aligned</option>
+      <option value="INPUT">same order as input file</option>
+    </param>
+
+    <conditional name="range">
+        <param name="mode" type="select" label="Output complete alignment (or specify part to output)">
+          <option value="complete">complete alignment</option>
+          <option value="part">only part of the alignment</option>
+        </param>
+        <when value="complete">
+        </when>
+        <when value="part">    
+           <param name="seq_range_start" size="5" type="integer" value="1" label="start point" help="sequence range to write">
+           </param>
+           <param name="seq_range_end" size="5" type="integer" value="99999" label="end point" >
+           </param> 
+        </when>
+    </conditional>
+   </page>
+  </inputs>
+  <outputs>
+    <data format="clustal" name="output"  label="${outname}_output.${outcontrol.outform}">
+       <change_format>
+           <when input="outcontrol.outform" value="phylip" format="phylip" />
+           <when input="outcontrol.outform" value="fasta" format="fasta" />
+       </change_format>
+    </data>
+    <data format="txt" name="outlog"  label="${outname}_clustal_log.txt"/>
+  </outputs>
+  <tests>
+     <test>
+        <param name="input" value="rgClustal_testin.fasta" />
+      <param name = "outname" value="" />
+      <param name = "outform" value="fasta" />
+      <param name = "dnarna" value="DNA" />
+      <param name = "mode" value="complete" />
+      <param name = "out_order" value="ALIGNED" />
+      <output name="output" file="rgClustal_testout.fasta" ftype="fasta" />
+      <output name="output" file="rgClustal_testout.log" ftype="txt" lines_diff="5" />
+     </test>
+  </tests>
+  <help>
+
+**Note**
+
+This tool allows you to run a multiple sequence alignment with ClustalW2 (see Clustsrc_) using the default options.
+ 
+For a tutorial introduction, see ClustalW2_
+
+You can align DNA or protein sequences in the input file which should be multiple sequences to be aligned in a fasta file
+
+A log will be output to your history showing the output Clustal would normally write to standard output.
+
+The alignments will appear as a clustal format file or optionally, as phylip or fasta format files in your history. If you choose fasta as 
+the output format, you can create a 'Logo' image using the Sequence Logo tool.
+
+If Clustal format is chosen, you have the option of adding basepair counts to the output
+
+A subsequence of the alignment can be output by setting the Output complete parameter to "Partial" and defining the offset and end of the subsequence to be output 
+
+----
+
+**Attribution**
+
+Clustal attribution and associated documentation are available at Clustsrc_
+
+The first iteration of this Galaxy wrapper was written by Hans-Rudolf Hotz - see Clustfirst_
+
+It was modified by Ross Lazarus for the rgenetics project
+
+This wrapper is now LGPL
+
+.. _ClustalW2: http://www.ebi.ac.uk/2can/tutorials/protein/clustalw.html  
+
+.. _Clustsrc: http://www.clustal.org
+
+.. _Clustfirst: http://lists.bx.psu.edu/pipermail/galaxy-dev/2010-November/003732.html
+
+  </help>
+
+</tool>
+
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/rgClustal_testin.fasta	Sun Dec 01 20:02:56 2013 -0500
@@ -0,0 +1,25 @@
+>c_briggsae-chrII(+)/43862-46313
+ATGAGCTTCCACAAAAGCATGAGCTTTCTCAGCTTCTGCCACATCAGCATTCAAATGATC
+>c_remanei-Crem_Contig172(-)/123228-124941
+ATGAGCCTCTACAACCGCATGATTCTTTTCAGCCTCTGCCACGTCCGCATTCAAATGCTC
+>c_brenneri-Cbre_Contig60(+)/627772-630087
+ATGAGCCTCCACAACAGCATGATTTTTCTCGGCTTCCGCCACATCCGCATTCAAATGATC
+>c_elegans-II(+)/9706834-9708803
+ATGAGCCTCTACTACAGCATGATTCTTCTCAGCTTCTGCAACGTCAGCATTCAGATGATC
+>c_briggsae-chrIfooI(+)/43862-46313
+CGCACAAATATGATGCACAAATCCACAACCTAAAGCATCTCCGATAACGTTGACCGAAGT
+>c_remanei-Crem_Contig172foo(-)/123228-124941
+AGCACAAATGTAATGAACGAATCCGCATCCCAACGCATCGCCAATCACATTCACAGATGT
+>c_brenneri-Cbre_Contig60gak(+)/627772-630087
+CGCACAAATGTAGTGGACAAATCCGCATCCCAAAGCGTCTCCGATAACATTTACCGAAGT
+>c_elegans-II(+)more/9706834-9708803
+TGCACAAATGTGATGAACGAATCCACATCCCAATGCATCACCGATCACATTGACAGATGT
+>c_briggsae-chrII(+)bar/43862-46313
+CCGGAGTCGATCCCTGAAT-----------------------------------------
+>c_remanei-Crem_Contig172zot(-)/123228-124941
+ACGAAGTCGGTCCCTATAAGGTATGATTTTATATGA----TGTACCATAAGGAAATAGTC
+>c_brenneri-Cbre_Contig60fee(+)/627772-630087
+ACGAAGTCGATCCCTGAAA---------TCAGATGAGCGGTTGACCA---GAGAACAACC
+>c_elegans-II(+)meh/9706834-9708803
+ACGAAGTCGGTCCCTGAAC--AATTATTT----TGA----TATA---GAAAGAAACGGTA
+
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/rgClustal_testout.fasta	Sun Dec 01 20:02:56 2013 -0500
@@ -0,0 +1,48 @@
+>c_briggsae-chrII_+_
+---ATGAGCTTCCACAAAAGCATGAGCTTT
+CTCAGCTTCTGCCACATCAGCATTCAAATG
+ATC
+>c_brenneri-Cbre_Contig60_+_
+---ATGAGCCTCCACAACAGCATGATTTTT
+CTCGGCTTCCGCCACATCCGCATTCAAATG
+ATC
+>c_remanei-Crem_Contig172_-_
+---ATGAGCCTCTACAACCGCATGATTCTT
+TTCAGCCTCTGCCACGTCCGCATTCAAATG
+CTC
+>c_elegans-II_+_
+---ATGAGCCTCTACTACAGCATGATTCTT
+CTCAGCTTCTGCAACGTCAGCATTCAGATG
+ATC
+>c_briggsae-chrII_+_bar
+---CCGGAGTCGATCCCTGAAT--------
+------------------------------
+---
+>c_brenneri-Cbre_Contig60fee_+_
+---ACGAAGTCGATCCCTGAAA--------
+-TCAGATGAGCGGTTGACCA---GAGAACA
+ACC
+>c_remanei-Crem_Contig172zot_-_
+---ACGAAGTCGGTCCCTATAAGGTATGAT
+TTTATATGA----TGTACCATAAGGAAATA
+GTC
+>c_elegans-II_+_meh
+---ACGAAGTCGGTCCCTGAAC--AATTAT
+TT----TGA----TATA---GAAAGAAACG
+GTA
+>c_briggsae-chrIfooI_+_
+CGCACAAATATGATGCACAAATCCACAACC
+TAAAGCATCTCCGATAACGTTGACCGAAGT
+---
+>c_brenneri-Cbre_Contig60gak_+_
+CGCACAAATGTAGTGGACAAATCCGCATCC
+CAAAGCGTCTCCGATAACATTTACCGAAGT
+---
+>c_remanei-Crem_Contig172foo_-_
+AGCACAAATGTAATGAACGAATCCGCATCC
+CAACGCATCGCCAATCACATTCACAGATGT
+---
+>c_elegans-II_+_more
+TGCACAAATGTGATGAACGAATCCACATCC
+CAATGCATCACCGATCACATTGACAGATGT
+---
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/rgClustal_testout.log	Sun Dec 01 20:02:56 2013 -0500
@@ -0,0 +1,112 @@
+
+
+
+ CLUSTAL 2.1 Multiple Sequence Alignments
+
+
+Sequence type explicitly set to DNA
+Sequence format is Pearson
+Sequence 1: c_briggsae-chrII_+_/43862-46313                 60 bp
+Sequence 2: c_remanei-Crem_Contig172_-_/123228-124941       60 bp
+Sequence 3: c_brenneri-Cbre_Contig60_+_/627772-630087       60 bp
+Sequence 4: c_elegans-II_+_/9706834-9708803                 60 bp
+Sequence 5: c_briggsae-chrIfooI_+_/43862-46313              60 bp
+Sequence 6: c_remanei-Crem_Contig172foo_-_/123228-124941    60 bp
+Sequence 7: c_brenneri-Cbre_Contig60gak_+_/627772-630087    60 bp
+Sequence 8: c_elegans-II_+_more/9706834-9708803             60 bp
+Sequence 9: c_briggsae-chrII_+_bar/43862-46313              60 bp
+Sequence 10: c_remanei-Crem_Contig172zot_-_/123228-124941    60 bp
+Sequence 11: c_brenneri-Cbre_Contig60fee_+_/627772-630087    60 bp
+Sequence 12: c_elegans-II_+_meh/9706834-9708803              60 bp
+Start of Pairwise alignments
+Aligning...
+
+Sequences (1:2) Aligned. Score:  80
+Sequences (1:3) Aligned. Score:  88
+Sequences (1:4) Aligned. Score:  83
+Sequences (1:5) Aligned. Score:  21
+Sequences (1:6) Aligned. Score:  20
+Sequences (1:7) Aligned. Score:  23
+Sequences (1:8) Aligned. Score:  18
+Sequences (1:9) Aligned. Score:  21
+Sequences (1:10) Aligned. Score:  16
+Sequences (1:11) Aligned. Score:  25
+Sequences (1:12) Aligned. Score:  10
+Sequences (2:3) Aligned. Score:  85
+Sequences (2:4) Aligned. Score:  86
+Sequences (2:5) Aligned. Score:  21
+Sequences (2:6) Aligned. Score:  20
+Sequences (2:7) Aligned. Score:  25
+Sequences (2:8) Aligned. Score:  20
+Sequences (2:9) Aligned. Score:  36
+Sequences (2:10) Aligned. Score:  16
+Sequences (2:11) Aligned. Score:  22
+Sequences (2:12) Aligned. Score:  17
+Sequences (3:4) Aligned. Score:  85
+Sequences (3:5) Aligned. Score:  13
+Sequences (3:6) Aligned. Score:  20
+Sequences (3:7) Aligned. Score:  25
+Sequences (3:8) Aligned. Score:  20
+Sequences (3:9) Aligned. Score:  36
+Sequences (3:10) Aligned. Score:  16
+Sequences (3:11) Aligned. Score:  18
+Sequences (3:12) Aligned. Score:  25
+Sequences (4:5) Aligned. Score:  13
+Sequences (4:6) Aligned. Score:  11
+Sequences (4:7) Aligned. Score:  20
+Sequences (4:8) Aligned. Score:  10
+Sequences (4:9) Aligned. Score:  31
+Sequences (4:10) Aligned. Score:  17
+Sequences (4:11) Aligned. Score:  29
+Sequences (4:12) Aligned. Score:  14
+Sequences (5:6) Aligned. Score:  73
+Sequences (5:7) Aligned. Score:  83
+Sequences (5:8) Aligned. Score:  80
+Sequences (5:9) Aligned. Score:  31
+Sequences (5:10) Aligned. Score:  14
+Sequences (5:11) Aligned. Score:  14
+Sequences (5:12) Aligned. Score:  12
+Sequences (6:7) Aligned. Score:  80
+Sequences (6:8) Aligned. Score:  88
+Sequences (6:9) Aligned. Score:  26
+Sequences (6:10) Aligned. Score:  16
+Sequences (6:11) Aligned. Score:  25
+Sequences (6:12) Aligned. Score:  12
+Sequences (7:8) Aligned. Score:  78
+Sequences (7:9) Aligned. Score:  31
+Sequences (7:10) Aligned. Score:  10
+Sequences (7:11) Aligned. Score:  12
+Sequences (7:12) Aligned. Score:  12
+Sequences (8:9) Aligned. Score:  31
+Sequences (8:10) Aligned. Score:  10
+Sequences (8:11) Aligned. Score:  14
+Sequences (8:12) Aligned. Score:  12
+Sequences (9:10) Aligned. Score:  63
+Sequences (9:11) Aligned. Score:  84
+Sequences (9:12) Aligned. Score:  78
+Sequences (10:11) Aligned. Score:  64
+Sequences (10:12) Aligned. Score:  76
+Sequences (11:12) Aligned. Score:  46
+Guide tree file created:   [/share/shared/galaxy/database/files/002/dataset_2705.dnd]
+
+There are 11 groups
+Start of Multiple Alignment
+
+Aligning...
+Group 1: Sequences:   2      Score:1045
+Group 2: Sequences:   2      Score:1016
+Group 3: Sequences:   4      Score:1001
+Group 4: Sequences:   2      Score:313
+Group 5: Sequences:   2      Score:731
+Group 6: Sequences:   4      Score:516
+Group 7: Sequences:   8      Score:344
+Group 8: Sequences:   2      Score:1016
+Group 9: Sequences:   2      Score:1054
+Group 10: Sequences:   4      Score:945
+Group 11: Sequences:  12      Score:380
+Alignment Score 6283
+firstres = 1 lastres = 63
+FASTA file created!
+
+Fasta-Alignment file created    [/share/shared/galaxy/database/files/002/dataset_2726.dat]
+
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/tool_dependencies.xml	Sun Dec 01 20:02:56 2013 -0500
@@ -0,0 +1,19 @@
+<?xml version="1.0" encoding="UTF-8"?>
+<tool_dependency>
+    <package name="clustalw" version="2.1">
+        <install version="1.0"> 
+           <actions>
+                <action type="download_by_url">http://www.clustal.org/download/current/clustalw-2.1.tar.gz</action>
+                <action type="shell_command">./configure &amp;&amp; make</action>
+                <action type="move_file"><source>src/clustalw2</source><destination>$INSTALL_DIR</destination></action>
+                <action type="set_environment">
+                    <environment_variable action="prepend_to" name="PATH">$INSTALL_DIR</environment_variable>
+                </action>
+           </actions>
+        </install>
+        <readme>Installs clustalw from http://www.clustal.org/download/current/clustalw-2.1.tar.gz
+       </readme>
+    </package>
+</tool_dependency>
+
+