annotate eggnog_mapper.xml @ 12:9d1fbff733cf draft

planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
author galaxyp
date Tue, 19 Jul 2022 15:14:52 +0000
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children 844fa988236b
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1 <tool id="eggnog_mapper" name="eggNOG Mapper" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@">
0
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2 <description>functional sequence annotation by orthology</description>
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3 <macros>
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4 <import>eggnog_macros.xml</import>
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5 </macros>
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6 <expand macro="requirements"/>
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7 <expand macro="version_command"/>
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8 <command detect_errors="aggressive"><![CDATA[
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9 #if $ortho_method.m == "no_search"
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10 cat
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11 #for aht in $ortho_method.annotate_hits_table
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12 $aht
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13 #end for
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14 > annotate_hits_table.tsv
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15 &&
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16 #end if
6
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17
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18 emapper.py
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19 --data_dir '$eggnog_data.fields.path'
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20 -m '$ortho_method.m'
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21
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22 #if $ortho_method.m in ['diamond', 'mmseqs', 'cache']:
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23 -i '$ortho_method.input'
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24 --itype '$ortho_method.input_trans.itype'
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25 #if $ortho_method.input_trans.itype in ['CDS', 'genome', 'metagenome']:
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26 $ortho_method.input_trans.translate
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27 #end if
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28 #if $ortho_method.input_trans.itype in ['genome', 'metagenome']:
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29 --genepred $ortho_method.input_trans.genepred
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30 #end if
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31 #elif $ortho_method.m == "no_search"
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32 --annotate_hits_table annotate_hits_table.tsv
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33 #end if
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35 #if $ortho_method.m == 'cache'
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36 --cache '$ortho_method.cache'
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37 #end if
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38
12
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39 #if $ortho_method.m in ['diamond', 'mmseqs']:
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40 ## Diamond option
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41 #if $ortho_method.m == "diamond":
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42 --matrix '$ortho_method.matrix_gapcosts.matrix'
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43 $ortho_method.matrix_gapcosts.gap_costs
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44 --sensmode $ortho_method.sensmode
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45 $ortho_method.dmnd_iterate
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46 $ortho_method.dmnd_ignore_warnings
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47 #elif $ortho_method.m == "mmseqs":
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48 --start_sens $ortho_method.start_sens
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49 --sens_steps $ortho_method.sens_steps
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50 --final_sens $ortho_method.final_sens
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51 #end if
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52
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53 ## Common options for search filtering (applies to diamond and mmseqs only)
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54 #if str($ortho_method.query_cover):
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55 --query_cover $ortho_method.query_cover
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56 #end if
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57 #if str($ortho_method.subject_cover):
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58 --subject_cover $ortho_method.subject_cover
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59 #end if
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60 #if str($ortho_method.pident):
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61 --pident $ortho_method.pident
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62 #end if
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63 #if str($ortho_method.evalue):
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64 --evalue $ortho_method.evalue
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65 #end if
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66 #if str($ortho_method.score):
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67 --score $ortho_method.score
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68 #end if
0
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69 #end if
12
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70
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71 #if $annotation_options.no_annot == "--no_annot"
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72 --no_annot
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73 #else
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74 #if str($annotation_options.seed_ortholog_evalue):
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75 --seed_ortholog_evalue $annotation_options.seed_ortholog_evalue
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76 #end if
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77 #if str($annotation_options.seed_ortholog_score):
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78 --seed_ortholog_score $annotation_options.seed_ortholog_score
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79 #end if
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80 #if $annotation_options.tax_scope:
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81 --tax_scope=$annotation_options.tax_scope
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82 #end if
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83 #if $annotation_options.target_orthologs:
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84 --target_orthologs=$annotation_options.target_orthologs
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85 #end if
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diff changeset
86 #if $annotation_options.go_evidence:
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87 --go_evidence=$annotation_options.go_evidence
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88 #end if
0
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89 #end if
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90 $output_options.no_file_comments
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91 $output_options.report_orthologs
12
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92 $output_options.md5
0
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93 --output='results'
1
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94 --cpu "\${GALAXY_SLOTS:-4}"
12
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95 --scratch_dir \${TEMP:-\$_GALAXY_JOB_TMP_DIR}
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96 --temp_dir \${TEMP:-\$_GALAXY_JOB_TMP_DIR}
0
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97 ]]></command>
6
3cb37af59360 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 2200885b5049b2d952959001c8a9b5ae5c62bee5"
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98 <inputs>
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99 <param name="eggnog_data" type="select" label="Version of eggNOG Database">
7
4e4c6329f6cd "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit fd234f7532b34a1b6ced0d3ac53a8f42348e23f7"
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100 <options from_data_table="eggnog_mapper_db_versioned">
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101 <filter type="static_value" column="3" value="@IDX_VERSION@" />
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102 </options>
6
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103 </param>
3cb37af59360 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 2200885b5049b2d952959001c8a9b5ae5c62bee5"
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104
12
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105 <conditional name="ortho_method">
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106 <param argument="-m" type="select" label="Method to search seed orthologs">
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107 <option value="diamond" selected="true">Diamond</option>
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108 <option value="mmseqs">MMseqs2</option>
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109 <option value="no_search">Skip search stage: annotate an existing seed orthologs data set</option>
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110 <option value="cache">Skip search stage: annotate based on cached annotations (see also --md5)</option>
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111 </param>
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112 <when value="diamond">
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113 <expand macro="fasta_input"/>
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114 <conditional name="matrix_gapcosts">
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115 <param argument="--matrix" type="select" label="Scoring matrix and gap costs">
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116 <option value="BLOSUM90">BLOSUM90</option>
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117 <option value="BLOSUM80">BLOSUM80</option>
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118 <option value="BLOSUM62" selected="true">BLOSUM62</option>
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119 <option value="BLOSUM50">BLOSUM50</option>
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120 <option value="BLOSUM45">BLOSUM45</option>
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121 <option value="PAM250">PAM250</option>
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122 <option value="PAM70">PAM70</option>
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123 <option value="PAM30">PAM30</option>
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124 </param>
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125 <when value="BLOSUM90">
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126 <param name="gap_costs" type="select" label="Gap Costs">
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127 <option value="--gapopen 9 --gapextend 2">Existence: 9 Extension: 2</option>
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128 <option value="--gapopen 8 --gapextend 2">Existence: 8 Extension: 2</option>
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129 <option value="--gapopen 7 --gapextend 2">Existence: 7 Extension: 2</option>
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130 <option value="--gapopen 6 --gapextend 2">Existence: 6 Extension: 2</option>
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131 <option value="--gapopen 11 --gapextend 1">Existence: 11 Extension: 1</option>
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132 <option value="--gapopen 10 --gapextend 1" selected="true">Existence: 10 Extension: 1</option>
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133 <option value="--gapopen 9 --gapextend 1">Existence: 9 Extension: 1</option>
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134 </param>
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135 </when>
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136 <when value="BLOSUM80">
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137 <param name="gap_costs" type="select" label="Gap Costs">
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138 <option value="--gapopen 8 --gapextend 2">Existence: 8 Extension: 2</option>
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139 <option value="--gapopen 7 --gapextend 2">Existence: 7 Extension: 2</option>
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140 <option value="--gapopen 6 --gapextend 2">Existence: 6 Extension: 2</option>
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141 <option value="--gapopen 11 --gapextend 1">Existence: 11 Extension: 1</option>
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142 <option value="--gapopen 10 --gapextend 1" selected="true">Existence: 10 Extension: 1</option>
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143 <option value="--gapopen 9 --gapextend 1">Existence: 9 Extension: 1</option>
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144 </param>
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145 </when>
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146 <when value="BLOSUM62">
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147 <param name="gap_costs" type="select" label="Gap Costs">
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148 <option value="--gapopen 11 --gapextend 2">Existence: 11 Extension: 2</option>
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149 <option value="--gapopen 10 --gapextend 2">Existence: 10 Extension: 2</option>
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150 <option value="--gapopen 9 --gapextend 2">Existence: 9 Extension: 2</option>
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151 <option value="--gapopen 8 --gapextend 2">Existence: 8 Extension: 2</option>
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diff changeset
152 <option value="--gapopen 7 --gapextend 2">Existence: 7 Extension: 2</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
153 <option value="--gapopen 6 --gapextend 2">Existence: 6 Extension: 2</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
154 <option value="--gapopen 13 --gapextend 1">Existence: 13 Extension: 1</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
155 <option value="--gapopen 12 --gapextend 1">Existence: 12 Extension: 1</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
156 <option value="--gapopen 11 --gapextend 1" selected="true">Existence: 11 Extension: 1</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
157 <option value="--gapopen 10 --gapextend 1">Existence: 10 Extension: 1</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
158 <option value="--gapopen 9 --gapextend 1">Existence: 9 Extension: 1</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
159 </param>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
160 </when>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
161 <when value="BLOSUM50">
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
162 <param name="gap_costs" type="select" label="Gap Costs">
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
163 <option value="--gapopen 13 --gapextend 3">Existence: 13 Extension: 3</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
164 <option value="--gapopen 12 --gapextend 3">Existence: 12 Extension: 3</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
165 <option value="--gapopen 11 --gapextend 3">Existence: 11 Extension: 3</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
166 <option value="--gapopen 10 --gapextend 3">Existence: 10 Extension: 3</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
167 <option value="--gapopen 9 --gapextend 3">Existence: 9 Extension: 3</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
168 <option value="--gapopen 16 --gapextend 2">Existence: 16 Extension: 2</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
169 <option value="--gapopen 15 --gapextend 2">Existence: 15 Extension: 2</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
170 <option value="--gapopen 14 --gapextend 2">Existence: 14 Extension: 2</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
171 <option value="--gapopen 13 --gapextend 2" selected="true">Existence: 13 Extension: 2</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
172 <option value="--gapopen 12 --gapextend 2">Existence: 12 Extension: 2</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
173 <option value="--gapopen 19 --gapextend 1">Existence: 19 Extension: 1</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
174 <option value="--gapopen 18 --gapextend 1">Existence: 18 Extension: 1</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
175 <option value="--gapopen 17 --gapextend 1">Existence: 17 Extension: 1</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
176 <option value="--gapopen 16 --gapextend 1">Existence: 16 Extension: 1</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
177 <option value="--gapopen 15 --gapextend 1">Existence: 15 Extension: 1</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
178 </param>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
179 </when>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
180 <when value="BLOSUM45">
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
181 <param name="gap_costs" type="select" label="Gap Costs">
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
182 <option value="--gapopen 13 --gapextend 3">Existence: 13 Extension: 3</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
183 <option value="--gapopen 12 --gapextend 3">Existence: 12 Extension: 3</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
184 <option value="--gapopen 11 --gapextend 3">Existence: 11 Extension: 3</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
185 <option value="--gapopen 10 --gapextend 3">Existence: 10 Extension: 3</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
186 <option value="--gapopen 15 --gapextend 2" selected="true">Existence: 15 Extension: 2</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
187 <option value="--gapopen 14 --gapextend 2">Existence: 14 Extension: 2</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
188 <option value="--gapopen 13 --gapextend 2">Existence: 13 Extension: 2</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
189 <option value="--gapopen 12 --gapextend 2">Existence: 12 Extension: 2</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
190 <option value="--gapopen 19 --gapextend 1">Existence: 19 Extension: 1</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
191 <option value="--gapopen 18 --gapextend 1">Existence: 18 Extension: 1</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
192 <option value="--gapopen 17 --gapextend 1">Existence: 17 Extension: 1</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
193 <option value="--gapopen 16 --gapextend 1">Existence: 16 Extension: 1</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
194 </param>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
195 </when>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
196 <when value="PAM250">
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
197 <param name="gap_costs" type="select" label="Gap Costs">
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
198 <option value="--gapopen 15 --gapextend 3">Existence: 15 Extension: 3</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
199 <option value="--gapopen 14 --gapextend 3">Existence: 14 Extension: 3</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
200 <option value="--gapopen 13 --gapextend 3">Existence: 13 Extension: 3</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
201 <option value="--gapopen 12 --gapextend 3">Existence: 12 Extension: 3</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
202 <option value="--gapopen 17 --gapextend 2">Existence: 17 Extension: 2</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
203 <option value="--gapopen 16 --gapextend 2">Existence: 16 Extension: 2</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
204 <option value="--gapopen 15 --gapextend 2">Existence: 15 Extension: 2</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
205 <option value="--gapopen 14 --gapextend 2" selected="true">Existence: 14 Extension: 2</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
206 <option value="--gapopen 13 --gapextend 2">Existence: 13 Extension: 2</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
207 <option value="--gapopen 21 --gapextend 1">Existence: 21 Extension: 1</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
208 <option value="--gapopen 20 --gapextend 1">Existence: 20 Extension: 1</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
209 <option value="--gapopen 19 --gapextend 1">Existence: 19 Extension: 1</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
210 <option value="--gapopen 18 --gapextend 1">Existence: 18 Extension: 1</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
211 <option value="--gapopen 17 --gapextend 1">Existence: 17 Extension: 1</option>
8
96cac424c870 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit e45c15081260025e470d23975ef5a734d3f8fc66"
galaxyp
parents: 7
diff changeset
212 </param>
12
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
213 </when>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
214 <when value="PAM70">
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
215 <param name="gap_costs" type="select" label="Gap Costs">
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
216 <option value="--gapopen 12 --gapextend 3">Existence: 12 Extension: 3</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
217 <option value="--gapopen 11 --gapextend 2">Existence: 11 Extension: 2</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
218 <option value="--gapopen 8 --gapextend 2">Existence: 8 Extension: 2</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
219 <option value="--gapopen 7 --gapextend 2">Existence: 7 Extension: 2</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
220 <option value="--gapopen 6 --gapextend 2">Existence: 6 Extension: 2</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
221 <option value="--gapopen 11 --gapextend 1">Existence: 11 Extension: 1</option>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
parents: 11
diff changeset
222 <option value="--gapopen 10 --gapextend 1" selected="true">Existence: 10 Extension: 1</option>
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223 <option value="--gapopen 9 --gapextend 1">Existence: 9 Extension: 1</option>
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224 </param>
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225 </when>
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226 <when value="PAM30">
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227 <param name="gap_costs" type="select" label="Gap Costs">
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228 <option value="--gapopen 15 --gapextend 3">Existence: 15 Extension: 3</option>
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229 <option value="--gapopen 13 --gapextend 3">Existence: 13 Extension: 3</option>
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230 <option value="--gapopen 14 --gapextend 2">Existence: 14 Extension: 2</option>
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231 <option value="--gapopen 7 --gapextend 2">Existence: 7 Extension: 2</option>
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232 <option value="--gapopen 6 --gapextend 2">Existence: 6 Extension: 2</option>
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233 <option value="--gapopen 5 --gapextend 2">Existence: 5 Extension: 2</option>
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234 <option value="--gapopen 14 --gapextend 1">Existence: 14 Extension: 1</option>
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235 <option value="--gapopen 10 --gapextend 1">Existence: 10 Extension: 1</option>
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236 <option value="--gapopen 9 --gapextend 1" selected="true">Existence: 9 Extension: 1</option>
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237 <option value="--gapopen 8 --gapextend 1">Existence: 8 Extension: 1</option>
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238 </param>
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239 </when>
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240 </conditional>
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241 <param argument="--sensmode" type="select" label="Diamond's sensitivity mode">
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242 <option value="default">default</option>
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243 <option value="fast">fast</option>
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244 <option value="mid-sensitive">mid-sensitive</option>
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245 <option value="sensitive" selected="true">sensitive</option>
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246 <option value="more-sensitive">more-sensitive</option>
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247 <option value="very-sensitive">very-sensitive</option>
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248 <option value="ultra-sensitive">ultra-sensitive</option>
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249 </param>
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250 <param argument="--dmnd_iterate" type="boolean" truevalue="--dmnd_iterate yes" falsevalue="--dmnd_iterate no" checked="false"
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251 label="Run diamond in iterative mode, up to the sensitivity level"/>
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252 <param argument="--dmnd_ignore_warnings" type="boolean" truevalue="--dmnd_ignore_warnings" falsevalue="" checked="false"
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253 label="Ignore Diamond warnings on sequence content (e.g. when a protein contains only ATGC symbols)"/>
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254 <expand macro="common_search_options"/>
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255 </when>
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256 <when value="mmseqs">
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257 <expand macro="fasta_input"/>
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258 <param argument="--start_sens" type="integer" value="3" min="0" max="100" label="Starting sensitivity" />
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259 <param argument="--sens_steps" type="integer" value="3" min="0" max="100" label="Number of sensitivity steps" />
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260 <param argument="--final_sens" type="integer" value="7" min="0" max="100" label="Final sensititivy step" />
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261 <expand macro="common_search_options"/>
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262 </when>
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263 <when value="no_search">
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264 <param argument="--annotate_hits_table" type="data" multiple="true" format="tabular" label="Seed orthologs">
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265 <validator type="expression" message="No seed orthologs">value.metadata.columns == 11</validator>
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266 <!-- would be cool to replace with this validator:
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267 <validator type="expression" message="No seed orthologs">value.metadata.column_names == '@SEED_ORTHOLOG_COLUMNS@'.split()</validator>
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268 but this does not work (yet) in tool tests since column_names can not be set in uploads -->
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269 </param>
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270 </when>
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271 <when value="cache">
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272 <expand macro="fasta_input"/>
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273 <param argument="--cache" type="data" multiple="true" format="tabular" label="EggNOG Annotations with md5 hashes" help="Annotations computed with EggNOG mapper with enabled --md5 option">
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274 <validator type="expression" message="No seed orthologs">value.metadata.columns == 22</validator>
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275 </param>
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276 <param name="output_no_annotations" type="boolean" checked="true" label="Output sequences without annotation" help="Produce an additional FASTA file with the sequences of queries for which an existing annotation was not found using cache mode. This file can be used as input of another eggNOG-mapper run without using the cache, trying to annotate the sequences."/>
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277 </when>
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278 </conditional>
6
3cb37af59360 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 2200885b5049b2d952959001c8a9b5ae5c62bee5"
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279
12
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280 <conditional name="annotation_options">
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281 <param argument="--no_annot" type="select" label="Annotate seed orthologs">
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282 <option value="">Yes</option>
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283 <option value="--no_annot">No</option>
0
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284 </param>
12
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285 <when value="">
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286 <param argument="--seed_ortholog_evalue" type="float" value="0.001" min="0" label="Min E-value threshold">
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287 <help>
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288 Min E-value expected when searching for seed eggNOG ortholog. Applies to phmmer/diamond searches.
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289 Queries not having a significant seed orthologs (E-value less than threshold) will not be annotated.
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290 </help>
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291 </param>
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292 <param argument="--seed_ortholog_score" type="float" optional="true" min="0" label="Minimum bit score threshold">
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293 <help>
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294 Min bit score expected when searching for seed eggNOG ortholog.
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295 Queries not having a significant seed orthologs will not be annotated.
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296 </help>
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297 </param>
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298 <param argument="--tax_scope" type="integer" optional="true" label="Set taxonomic scope" help="NCBI taxonomy id" />
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299 <param argument="--target_orthologs" type="select" label="target orthologs for functional transfer">
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300 <option value="one2one">one2one</option>
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301 <option value="many2one">many2one</option>
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302 <option value="one2many">one2many</option>
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303 <option value="many2many">many2many</option>
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304 <option value="all" selected="true">all</option>
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305 </param>
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306 <param argument="--go_evidence" type="select"
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307 label="Select the set of GO terms that should be used for annotation">
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308 <option value="experimental">experimental = Use only terms inferred from experimental evidence</option>
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309 <option value="non-electronic" selected="true">non-electronic = Use only non-electronically curated terms</option>
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310 <option value="all" selected="true">All (experimental + non-electronic)</option>
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311 </param>
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312 </when>
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313 <when value="--no_annot"/>
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314 </conditional>
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315
0
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316 <section name="output_options" expanded="false" title="Output Options">
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317 <param argument="--no_file_comments" type="boolean" truevalue="--no_file_comments" falsevalue="" checked="true"
0
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318 label="Exclude header lines and stats from output files"/>
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319 <param argument="--report_orthologs" type="boolean" truevalue="--report_orthologs" falsevalue="" checked="false"
0
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320 label="Output a file with the list of orthologs for each hits"/>
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321 <param argument="--md5" type="boolean" truevalue="--md5" falsevalue="" checked="false"
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322 label="Add md5 hash of each query to annotations"/>
0
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323 </section>
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324 </inputs>
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325 <outputs>
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326 <data name="seed_orthologs" format="tabular" label="${tool.name} on ${on_string}: seed_orthologs" from_work_dir="results.emapper.seed_orthologs">
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327 <filter>ortho_method['m'] not in ['no_search', 'cache']</filter>
0
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328 <actions>
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329 <action name="column_names" type="metadata" default="@SEED_ORTHOLOG_COLUMNS@"/>
0
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330 </actions>
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331 </data>
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332 <data name="annotations" format="tabular" label="${tool.name} on ${on_string}: annotations" from_work_dir="results.emapper.annotations">
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333 <filter>annotation_options['no_annot'] == ''</filter>
0
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334 <actions>
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335 <conditional name="output_options.md5">
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336 <when value="True">
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337 <action name="column_names" type="metadata" default="query,seed_ortholog,evalue,score,max_annot_lvl,COG_category,Description,Preferred_name,GOs,EC,KEGG_ko,KEGG_Pathway,KEGG_Module,KEGG_Reaction,KEGG_rclass,BRITE,KEGG_TC,CAZy,BiGG_Reaction,PFAMseggNOG_OGs,md5"/>
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338 </when>
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339 <when value="False">
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340 <action name="column_names" type="metadata" default="query,seed_ortholog,evalue,score,max_annot_lvl,COG_category,Description,Preferred_name,GOs,EC,KEGG_ko,KEGG_Pathway,KEGG_Module,KEGG_Reaction,KEGG_rclass,BRITE,KEGG_TC,CAZy,BiGG_Reaction,PFAMseggNOG_OGs"/>
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341 </when>
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342 </conditional>
0
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343 </actions>
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344 </data>
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345 <data name="annotations_orthologs" format="tabular" label="${tool.name} on ${on_string}: orthologs" from_work_dir="results.emapper.orthologs">
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346 <filter>ortho_method['m'] != 'cache' and output_options['report_orthologs']</filter>
0
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347 <actions>
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348 <action name="column_names" type="metadata" default="query,orth_type,species,orthologs"/>
0
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349 </actions>
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350 </data>
12
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351 <data name="no_annotations" format="fasta" label="${tool.name} on ${on_string}: Sequences wo annotation" from_work_dir="results.emapper.no_annotations.fasta">
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352 <filter>ortho_method['m'] == 'cache' and output_options['output_no_annotations']</filter>
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353 </data>
0
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354 </outputs>
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355 <tests>
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356 <!-- test producing only seed orthologs-->
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357 <test expect_num_outputs="1">
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358 <param name="eggnog_data" value="@EGGNOG_DB_VERSION@"/>
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359 <conditional name="ortho_method">
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360 <param name="input" value="Nmar_0135.fa" ftype="fasta"/>
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361 </conditional>
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362 <conditional name="annotation_options">
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363 <param name="no_annot" value="--no_annot"/>
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364 </conditional>
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365 <section name="output_options">
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366 <param name="no_file_comments" value="true"/>
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367 </section>
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368 <expand macro="seed_orthologs_assertion"/>
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369 <expand macro="stdout_assertion"/>
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370 </test>
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371
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372 <!-- test producing annotations form seed orthologs -->
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373 <test expect_num_outputs="2">
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374 <param name="eggnog_data" value="@EGGNOG_DB_VERSION@"/> <!-- not passed in test, but required for test to work -->
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375 <conditional name="ortho_method">
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376 <param name="m" value="no_search"/>
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377 <param name="annotate_hits_table" value="DIA_nlim.emapper.seed_orthologs" ftype="tabular">
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378 <!-- this has no effect at the moment since column_names can not be set in uploads <metadata name="column_names" value="@SEED_ORTHOLOG_COLUMNS@"/> -->
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379 </param>
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380 </conditional>
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381 <conditional name="annotation_options">
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382 </conditional>
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383 <section name="output_options">
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384 <param name="report_orthologs" value="true"/>
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385 <param name="no_file_comments" value="true"/>
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386 </section>
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387 <expand macro="annotations_assertion"/>
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388 <expand macro="annotations_orthologs_assertion"/>
11
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389 <expand macro="stdout_assertion"/>
0
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390 </test>
12
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391
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392 <!-- test producing seed orthologs and annotations at once (ie the same as the previous two tests but in a single tool run) -->
11
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393 <test expect_num_outputs="3">
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394 <param name="eggnog_data" value="@EGGNOG_DB_VERSION@"/> <!-- not passed in test, but required for test to work -->
12
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395 <conditional name="ortho_method">
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396 <param name="input" value="Nmar_0135.fa" ftype="fasta"/>
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397 </conditional>
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398 <section name="output_options">
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399 <param name="report_orthologs" value="true"/>
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400 <param name="no_file_comments" value="true"/>
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401 <param name="md5" value="true"/>
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402 </section>
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403 <expand macro="seed_orthologs_assertion"/>
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404 <expand macro="annotations_assertion" columns="22" add_metadata_columm_names=",md5" add_column_names="&#009;md5" add_column_re="\t[\d\w]+"/>
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405 <expand macro="annotations_orthologs_assertion"/>
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406 <expand macro="stdout_assertion"/>
6
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407 </test>
12
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diff changeset
408
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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409 <!-- test using chached annotations from previous run -->
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410 <test expect_num_outputs="2">
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411 <param name="eggnog_data" value="@EGGNOG_DB_VERSION@"/> <!-- not passed in test, but required for test to work -->
12
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412 <conditional name="ortho_method">
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413 <param name="m" value="cache"/>
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414 <param name="input" value="Nmar_0135.fa" ftype="fasta"/>
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415 <param name="cache" value="DIA_nlim.emapper.annotations_cached" ftype="tabular"/>
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416 </conditional>
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417 <section name="output_options">
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418 <param name="report_orthologs" value="true"/>
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419 <param name="no_file_comments" value="true"/>
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420 <param name="md5" value="true"/>
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421 </section>
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422 <expand macro="annotations_assertion" columns="22" add_metadata_columm_names=",md5" add_column_names="&#009;md5" add_column_re="\t[\d\w]+"/>
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423 <output name="no_annotations" ftype="fasta">
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424 <assert_contents>
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425 <has_n_lines n="0"/>
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426 </assert_contents>
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427 </output>
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428 <expand macro="stdout_assertion"/>
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429 </test>
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430
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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431 <!-- test setting tax scope-->
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432 <test expect_num_outputs="3">
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433 <param name="eggnog_data" value="@EGGNOG_DB_VERSION@"/> <!-- not passed in test, but required for test to work -->
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diff changeset
434 <conditional name="ortho_method">
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435 <param name="input" value="Nmar_0135.fa" ftype="fasta"/>
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436 </conditional>
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437 <section name="annotation_options">
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438 <param name="tax_scope" value="651137" />
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439 </section>
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440 <section name="output_options">
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441 <param name="report_orthologs" value="true"/>
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442 <param name="no_file_comments" value="true"/>
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443 </section>
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diff changeset
444 <expand macro="seed_orthologs_assertion"/>
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445 <expand macro="annotations_assertion"/>
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diff changeset
446 <expand macro="annotations_orthologs_assertion"/>
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447 <expand macro="stdout_assertion">
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diff changeset
448 <has_text text="--tax_scope=651137"/>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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diff changeset
449 </expand>
0
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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450 </test>
12
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diff changeset
451
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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diff changeset
452 <!-- test setting a diamond option-->
11
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453 <test expect_num_outputs="3">
8
96cac424c870 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit e45c15081260025e470d23975ef5a734d3f8fc66"
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diff changeset
454 <param name="eggnog_data" value="@EGGNOG_DB_VERSION@"/> <!-- not passed in test, but required for test to work -->
12
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diff changeset
455 <conditional name="ortho_method">
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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diff changeset
456 <param name="m" value="diamond" />
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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diff changeset
457 <param name="input" value="Nmar_0135.fa" ftype="fasta"/>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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diff changeset
458 <param name="sensmode" value="fast" />
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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diff changeset
459 </conditional>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
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diff changeset
460 <section name="output_options">
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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diff changeset
461 <param name="report_orthologs" value="true"/>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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diff changeset
462 <param name="no_file_comments" value="true"/>
8
96cac424c870 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit e45c15081260025e470d23975ef5a734d3f8fc66"
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diff changeset
463 </section>
12
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diff changeset
464 <expand macro="seed_orthologs_assertion"/>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
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diff changeset
465 <expand macro="annotations_assertion"/>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
galaxyp
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diff changeset
466 <expand macro="annotations_orthologs_assertion"/>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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diff changeset
467 <expand macro="stdout_assertion">
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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diff changeset
468 <has_text text="--sensmode fast"/>
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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diff changeset
469 </expand>
8
96cac424c870 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit e45c15081260025e470d23975ef5a734d3f8fc66"
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diff changeset
470 </test>
96cac424c870 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit e45c15081260025e470d23975ef5a734d3f8fc66"
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diff changeset
471 <!-- not enabled as it requires a specific .db file, hard to minimize -->
96cac424c870 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit e45c15081260025e470d23975ef5a734d3f8fc66"
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diff changeset
472 <!--test>
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diff changeset
473 <param name="eggnog_data" value="@EGGNOG_DB_VERSION@"/>
8
96cac424c870 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit e45c15081260025e470d23975ef5a734d3f8fc66"
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474 <param name="input" value="Nmar_0135.fa" ftype="fasta"/>
96cac424c870 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit e45c15081260025e470d23975ef5a734d3f8fc66"
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diff changeset
475 <section name="seed_ortho_options">
96cac424c870 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit e45c15081260025e470d23975ef5a734d3f8fc66"
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diff changeset
476 <conditional name="ortho_method">
96cac424c870 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit e45c15081260025e470d23975ef5a734d3f8fc66"
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diff changeset
477 <param name="m" value="mmseqs" />
96cac424c870 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit e45c15081260025e470d23975ef5a734d3f8fc66"
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diff changeset
478 <param name="start_sens" value="4" />
96cac424c870 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit e45c15081260025e470d23975ef5a734d3f8fc66"
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479 </conditional>
96cac424c870 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit e45c15081260025e470d23975ef5a734d3f8fc66"
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480 </section>
96cac424c870 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit e45c15081260025e470d23975ef5a734d3f8fc66"
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diff changeset
481 <param name="report_orthologs" value="true"/>
96cac424c870 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit e45c15081260025e470d23975ef5a734d3f8fc66"
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diff changeset
482 <param name="no_file_comments" value="true"/>
96cac424c870 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit e45c15081260025e470d23975ef5a734d3f8fc66"
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diff changeset
483 <output name="seed_orthologs" file="DIA_nlim.emapper.seed_orthologs" ftype="tabular" compare="sim_size"/>
96cac424c870 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit e45c15081260025e470d23975ef5a734d3f8fc66"
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diff changeset
484 <output name="annotations" file="DIA_nlim.emapper.annotations" ftype="tabular" compare="sim_size"/>
96cac424c870 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit e45c15081260025e470d23975ef5a734d3f8fc66"
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diff changeset
485 <output name="annotations_orthologs" file="DIA_nlim.emapper.annotations_orthologs" ftype="tabular"/>
96cac424c870 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit e45c15081260025e470d23975ef5a734d3f8fc66"
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diff changeset
486 </test-->
0
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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diff changeset
487 </tests>
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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diff changeset
488 <help><![CDATA[
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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diff changeset
489
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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diff changeset
490 eggnog-mapper
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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diff changeset
491 =============
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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diff changeset
492 Overview
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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diff changeset
493 --------
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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diff changeset
494
6
3cb37af59360 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 2200885b5049b2d952959001c8a9b5ae5c62bee5"
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diff changeset
495 ``eggnog-mapper`` is a tool for fast functional annotation of novel sequences (genes or proteins) using precomputed eggNOG-based orthology assignments.
3cb37af59360 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 2200885b5049b2d952959001c8a9b5ae5c62bee5"
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496 Obvious examples include the annotation of novel genomes, transcriptomes or even metagenomic gene catalogs.
3cb37af59360 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 2200885b5049b2d952959001c8a9b5ae5c62bee5"
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diff changeset
497 The use of orthology predictions for functional annotation is considered more precise than traditional homology searches,
0
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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498 as it avoids transferring annotations from paralogs (duplicate genes with a higher chance of being involved in functional divergence).
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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diff changeset
499
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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500 EggNOG-mapper is also available as a public online resource: `<http://beta-eggnogdb.embl.de/#/app/emapper>`_.
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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diff changeset
501
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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diff changeset
502 Outputs
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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503 -------
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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diff changeset
504
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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diff changeset
505 **seed_orthologs**
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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diff changeset
506
6
3cb37af59360 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 2200885b5049b2d952959001c8a9b5ae5c62bee5"
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diff changeset
507 each line in the file provides the best match of each query within the best Orthologous Group (OG)
3cb37af59360 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 2200885b5049b2d952959001c8a9b5ae5c62bee5"
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diff changeset
508 reported in the [project].hmm_hits file, obtained running PHMMER against all sequences within the best OG.
3cb37af59360 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 2200885b5049b2d952959001c8a9b5ae5c62bee5"
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diff changeset
509 The seed ortholog is used to fetch fine-grained orthology relationships from eggNOG.
3cb37af59360 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 2200885b5049b2d952959001c8a9b5ae5c62bee5"
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diff changeset
510 If using the diamond search mode, seed orthologs are directly
0
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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diff changeset
511 obtained from the best matching sequences by running DIAMOND against the whole eggNOG protein space.
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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diff changeset
512
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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513 **annotations**
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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diff changeset
514
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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diff changeset
515 This file provides final annotations of each query. Tab-delimited columns in the file are:
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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diff changeset
516
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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517 - ``query_name``: query sequence name
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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518 - ``seed_eggNOG_ortholog``: best protein match in eggNOG
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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diff changeset
519 - ``seed_ortholog_evalue``: best protein match (e-value)
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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520 - ``seed_ortholog_score``: best protein match (bit-score)
6
3cb37af59360 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 2200885b5049b2d952959001c8a9b5ae5c62bee5"
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diff changeset
521 - ``predicted_taxonomic_group``
3cb37af59360 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 2200885b5049b2d952959001c8a9b5ae5c62bee5"
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522 - ``predicted_protein_name``: Predicted protein name for query sequences
0
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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523 - ``GO_terms``: Comma delimited list of predicted Gene Ontology terms
6
3cb37af59360 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 2200885b5049b2d952959001c8a9b5ae5c62bee5"
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diff changeset
524 - ``EC_number``
3cb37af59360 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 2200885b5049b2d952959001c8a9b5ae5c62bee5"
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525 - ``KEGG_KO``
3cb37af59360 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 2200885b5049b2d952959001c8a9b5ae5c62bee5"
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526 - ``KEGG_Pathway``: Comma delimited list of predicted KEGG pathways
3cb37af59360 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 2200885b5049b2d952959001c8a9b5ae5c62bee5"
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527 - ``KEGG_Module``
3cb37af59360 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 2200885b5049b2d952959001c8a9b5ae5c62bee5"
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528 - ``KEGG_Reaction``
3cb37af59360 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 2200885b5049b2d952959001c8a9b5ae5c62bee5"
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529 - ``KEGG_rclass``
3cb37af59360 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 2200885b5049b2d952959001c8a9b5ae5c62bee5"
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530 - ``BRITE``
3cb37af59360 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 2200885b5049b2d952959001c8a9b5ae5c62bee5"
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531 - ``KEGG_TC``
3cb37af59360 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 2200885b5049b2d952959001c8a9b5ae5c62bee5"
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532 - ``CAZy``
3cb37af59360 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 2200885b5049b2d952959001c8a9b5ae5c62bee5"
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533 - ``BiGG_Reactions``
0
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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534 - ``Annotation_tax_scope``: The taxonomic scope used to annotate this query sequence
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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535 - ``Matching_OGs``: Comma delimited list of matching eggNOG Orthologous Groups
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3cb37af59360 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 2200885b5049b2d952959001c8a9b5ae5c62bee5"
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536 - ``best_OG|evalue|score``: Best matching Orthologous Groups (deprecated, use smallest from eggnog OGs)
3cb37af59360 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 2200885b5049b2d952959001c8a9b5ae5c62bee5"
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537 - ``COG_functional_categories``: COG functional category inferred from best matching OG
3cb37af59360 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 2200885b5049b2d952959001c8a9b5ae5c62bee5"
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538 - ``eggNOG_free_text_description``
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f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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539
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540 **Recommentation for large input data**
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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541
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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542 EggNOG-mapper consists of two phases
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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543
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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544 1. finding seed orthologous sequences (compute intensive)
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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545 2. expanding annotations (IO intensive)
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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546
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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547 by default (i.e. if *Method to search seed orthologs* is not *Skip search stage...* and *Annotate seed orthologs* is *Yes*)
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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548 both phases are executed within one tool run.
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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549
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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550 For large input FASTA datasets in can be favourable to split this in two separate
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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551 tool runs as follows:
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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552
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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553 1. Split the FASTA (e.g. 1M seqs per data set)
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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554 2. Run the search phase only (set *Annotate seed orthologs* to *No*) on the separate FASTA files.
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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555 3. Run the annotation phase (set *Method to search seed orthologs* to *Skip search stage...*)
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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556
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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557 See [also](https://github.com/eggnogdb/eggnog-mapper/wiki/eggNOG-mapper-v2.1.5-to-v2.1.8#Setting_up_large_annotation_jobs)
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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558
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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559 Another alternative is to use cached annotations (produced in a run with --md5 enabled).
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560
9d1fbff733cf planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit 07877ba6d3fb6e28e94500f2392db6393cf325fd
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561
0
f4e5279b5d10 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper commit 9dbc338105e83091a636166030f618ec881c5f6f
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562 ]]></help>
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a5a3bdd0954b "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/eggnog_mapper/eggnog_mapper commit ba81f4bfe31157aa6b986e81d5e4405ae1372b3b"
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563 <expand macro="citations"/>
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564 </tool>