Mercurial > repos > iuc > bctools_remove_spurious_events
annotate rm_spurious_events.xml @ 2:84aff696aa97 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit 758b42538f722759e7b5414f64c9a7973cc221b4"
author | iuc |
---|---|
date | Wed, 25 Dec 2019 11:39:45 -0500 |
parents | 9ad7bf370022 |
children | 5c72eb992651 |
rev | line source |
---|---|
2
84aff696aa97
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit 758b42538f722759e7b5414f64c9a7973cc221b4"
iuc
parents:
0
diff
changeset
|
1 <tool id="bctools_remove_spurious_events" name="Remove spurious" version="@VERSION@+galaxy1"> |
0
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
2 <description>crosslinking events</description> |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
3 <macros> |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
4 <import>macros.xml</import> |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
5 </macros> |
2
84aff696aa97
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit 758b42538f722759e7b5414f64c9a7973cc221b4"
iuc
parents:
0
diff
changeset
|
6 <expand macro="requirements"> |
84aff696aa97
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit 758b42538f722759e7b5414f64c9a7973cc221b4"
iuc
parents:
0
diff
changeset
|
7 <requirement type="package" version="8.31">coreutils</requirement> |
84aff696aa97
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit 758b42538f722759e7b5414f64c9a7973cc221b4"
iuc
parents:
0
diff
changeset
|
8 </expand> |
0
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
9 <command detect_errors="exit_code"><![CDATA[ |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
10 rm_spurious_events.py |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
11 '$events' |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
12 --threshold '$threshold' |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
13 --outfile '$events_filtered' |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
14 ]]></command> |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
15 <inputs> |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
16 <param name="events" type="data" format="bed" label="BED6 file containing alignments"/> |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
17 <param name="threshold" argument="--threshold" type="float" value="0.1" optional="true" label="Threshold for spurious event removal" help="Remove all crosslinking events that combine less reads than this fraction of the maximum number of reads at this position."/> |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
18 </inputs> |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
19 <outputs> |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
20 <data name="events_filtered" format="bed"/> |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
21 </outputs> |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
22 <tests> |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
23 <test> |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
24 <param name="events" value="merged_pcr_dupes_spurious.bed"/> |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
25 <param name="threshold" value="0.5"/> |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
26 <output name="events_filtered" file="merged_pcr_dupes_spurious_filtered_thresh05.bed"/> |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
27 </test> |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
28 </tests> |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
29 <help><![CDATA[ |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
30 |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
31 bctools - Remove spurious events originating from errors in unique molecular identifiers (UMIs) |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
32 =============================================================================================== |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
33 |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
34 This tool compares all events sharing the same coordinates. Among each group |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
35 of events the maximum number of PCR duplicates is determined. By default, all events that |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
36 are supported by less than 10 percent of this maximum count are removed. |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
37 |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
38 Input |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
39 ----- |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
40 |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
41 * BED6 containing crosslinking events with score field set to number of PCR duplicates |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
42 |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
43 Output |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
44 ------ |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
45 |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
46 * BED6 with spurious crosslinking events removed, sorted by fields chrom, start, stop, strand |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
47 |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
48 ]]></help> |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
49 <expand macro="citations"/> |
9ad7bf370022
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit ae3b9baaf7040ed8b165d17466b8b2fe016d3d48
iuc
parents:
diff
changeset
|
50 </tool> |