annotate hyphy_relax.xml @ 23:801547975424 draft

"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/hyphy/ commit 2742ee3b4e90f65352845265d2f85c4263e0eabb"
author iuc
date Tue, 20 Apr 2021 10:32:25 +0000
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1 <?xml version="1.0"?>
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2 <tool id="hyphy_relax" name="HyPhy-RELAX" version="@VERSION@+galaxy0" profile="19.09">
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3 <description>Detect relaxed selection in a codon-based
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4 phylogenetic framework</description>
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5 <macros>
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6 <import>macros.xml</import>
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7 <token name="@OPERATION@">RELAX</token>
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8 <token name="@operation@">relax</token>
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9 </macros>
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10 <expand macro="requirements"/>
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11 <command detect_errors="exit_code"><![CDATA[
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12 @SYMLINK_FILES@
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13 export OMP_NUM_THREADS="\${GALAXY_SLOTS:-1}" &&
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14 hyphy relax
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15 --alignment ./$input_file
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16 #if $input_nhx:
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17 --tree ./input.nhx
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18 #end if
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19 --models '$analysisType'
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20 --code '$gencodeid'
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21 #if $treeAnnotations == "2":
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22 --test TEST
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23 --reference REFERENCE
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24 #else:
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25 --test TEST
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26 #end if
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27 > '$relax_log' ;
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28 @CATCH_ERROR@
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29 ]]></command>
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30 <inputs>
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31 <expand macro="inputs"/>
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32 <expand macro="gencode"/>
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33 <param argument="--model" name="analysisType" type="select" label="Analysis Type" >
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34 <option value="All">All</option>
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35 <option value="Minimal">Minimal</option>
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36 </param>
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37 <param name="treeAnnotations" type="select" label="Tree Annotations" help = "Is there one or two sets of annotated branches in the Newick file?">
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38 <option value="1">TEST only</option>
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39 <option value="2">TEST and REFERENCE</option>
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40 </param>
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41 </inputs>
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42 <outputs>
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43 <data name="relax_log" format="txt"/>
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44 <data name="relax_output" format="hyphy_results.json"/>
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45 </outputs>
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46 <tests>
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47 <test>
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48 <param name="input_file" ftype="fasta" value="relax-in1.fa"/>
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49 <param name="input_nhx" ftype="nhx" value="relax-in1.nhx"/>
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50 <output name="relax_output" file="relax-out1.json" compare="sim_size"/>
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51 </test>
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52 </tests>
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53 <help><![CDATA[
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54 RELAX: a test for selection differences
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55 =======================================
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56
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57 What question does this method answer?
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58 --------------------------------------
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59
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60 Is there evidence the strength of selection has been relaxed (or conversely intensified) on a specified group of lineages (TEST)
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61 relative to a set of reference lineages (RELAX)? Importantly, RELAX is not designed to detect diversifying selection specifically.
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62 We note that the RELAX framework can perform both this specific hypothesis test as well as fit a suite of descriptive models which address,
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63 or example, overall rate differences between test and reference branches or lineage-specific inferences of selection relaxation.
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64
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65 Recommended Applications
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66 ------------------------
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67
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68 1. Testing for a systematic shift (relaxation / intensification) in the distribution of selection pressure associated with major biological transitions such as hosting switching in viruses, lifestyle evolution in bacteria (i.e. transition from free-living to endosymbiotic lifestyle
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69 2. Comparing selective regimes between two subsets of branches in the tree, e.g. to investigate selective differences due an environmental/phenotypic changes.
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70
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71
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72 Brief description
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73 -----------------
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74
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75 RELAX (a random effects test of selection relaxation) uses a random
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76 effects branch-site model framework to test whether a set of 'Test'
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77 branches evolves under relaxed selection relative to a set of
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78 'Reference' branches (R), as measured by the relaxation parameter (K).
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79
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80 Input
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81 -----
1
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82
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83 1. A *FASTA* sequence alignment.
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84 2. A phylogenetic tree in the *Newick* format that is further partitioned into one or two groups (_TEST_ or _TEST_ and _REFERENCE_). See http://hyphy.org/tutorials/phylotree/
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85
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86 Note: the names of sequences in the alignment must match the names of the sequences in the tree.
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87
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88
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89 Output
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90 ------
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91
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92 A JSON file with analysis results (http://hyphy.org/resources/json-fields.pdf).
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93
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94 A custom visualization module for viewing these results is available (see http://vision.hyphy.org/RELAX for an example)
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95
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96 Further reading
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97 ---------------
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98
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99 http://hyphy.org/methods/selection-methods/#RELAX
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100
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101
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102 Tool options
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103 ------------
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104 ::
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105
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106
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107 --code Which genetic code to use
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108
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109 --model Which type of test to run
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110 All : fit 4 different models [default]
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111 Minimal: only perform a 2-model test of relaxation/intensification [faster]
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112
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113
0
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114 ]]></help>
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115 <expand macro="citations">
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116 <citation type="doi">10.1093/molbev/msu400</citation>
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117 </expand>
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118 </tool>