Mercurial > repos > iuc > icescreen
changeset 4:c5f7c311f1b0 draft
planemo upload for repository https://forgemia.inra.fr/ices_imes_analysis/icescreen commit a9bc15365abc70c0367d732b790763d8fa53b220
| author | iuc |
|---|---|
| date | Tue, 28 Feb 2023 14:10:38 +0000 |
| parents | 2bb38197ff75 |
| children | 28e82853c217 |
| files | icescreen.xml test-data/NC_013798_298468_322494_NC_020450_643089_661957_detected_ME.summary test-data/NC_013798_298468_322494_NC_020450_643089_661957_detected_ME.tsv test-data/genbank/NC_013798_298468_322494_NC_020450_643089_661957.gb |
| diffstat | 4 files changed, 1831 insertions(+), 3 deletions(-) [+] |
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--- a/icescreen.xml Tue Dec 06 10:40:53 2022 +0000 +++ b/icescreen.xml Tue Feb 28 14:10:38 2023 +0000 @@ -1,7 +1,7 @@ <tool id="icescreen" name="ICEscreen" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="20.09"> <description>detects and annotates ICEs (Integrative and Conjugative Elements) and IMEs (Integrative and Mobilizable Elements) in Firmicutes genomes.</description> <macros> - <token name="@TOOL_VERSION@">1.1.0</token> + <token name="@TOOL_VERSION@">1.1.1</token> <token name="@VERSION_SUFFIX@">0</token> </macros> <requirements> @@ -45,7 +45,7 @@ ]]> </command> <inputs> - <param name="genome" type="data" format="genbank" label="Input genomes to analyze in Genbank format" help="Each Genbank file can only contain ONE record, multi-genbank files are not supported. Each Genbank file must include the ORIGIN nucleotide sequence at the end"/> + <param name="genome" type="data" format="genbank" label="Input genomes to analyze in Genbank format" help="Multi-genbank files (i.e. gbff) are supported. Each record must include the ORIGIN nucleotide sequence at the end."/> <section name="taxonomy" title="Taxonomy of the genomes to analyse" expanded="False"> <param name="mode" type="select" label="Firmicutes is the defaut parameter" help="Streptomyces is EXPERIMENTAL and is NOT recommended."> <option value="firmicutes" selected="true">Firmicutes</option> @@ -115,7 +115,6 @@ <test expect_num_outputs="4"> <param name="genome" value="genbank/NC_004668_137848_164286.gb" ftype="genbank" /> <param name="optional_files" value="output_zip_all_files" /> - <output name="outzip" > <assert_contents> <has_archive_member path=".*/*_detected_SP_withMEIds.tsv"><has_text text="ICE_IME_id" /></has_archive_member> @@ -156,6 +155,18 @@ </assert_contents> </output> </test> + <test expect_num_outputs="3"> + <param name="genome" value="genbank/NC_013798_298468_322494_NC_020450_643089_661957.gb" ftype="genbank" /> + <output name="summary" file="NC_013798_298468_322494_NC_020450_643089_661957_detected_ME.summary" ftype="txt" /> + <output name="detected_me" file="NC_013798_298468_322494_NC_020450_643089_661957_detected_ME.tsv" ftype="tabular" /> + <output name="detected_sp" > + <assert_contents> + <has_text text="ICE_IME_id" /> + <has_text text="WP_044555479.1" /> + <has_text text="WP_015426013.1" /> + </assert_contents> + </output> + </test> </tests> <help><![CDATA[ .. class:: warningmark
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/NC_013798_298468_322494_NC_020450_643089_661957_detected_ME.summary Tue Feb 28 14:10:38 2023 +0000 @@ -0,0 +1,82 @@ + +##################### Main parameters of the ICEscreen analysis ##################### +Maximum distance in CDS between consecutive SPs within a segments: 100 +Maximum distance in CDS between consecutive SPs within an IME element: 14 + + +##################### ICEscreen statistics for genome accession NC_013798.1 ##################### + +##### Mobile elements ##### +## Number of complete elements +Complete ICE (4 types of SP): 1 +Complete IME (R+I, R+C+I with distance between consecutive SPs <= 14 CDSs): 0 +## Number of complete modules +Conjugation module (R+C+V): 0 +Mobilizable element (R+C with distance between consecutive SPs <= 14 CDS): 0 +## Number of partial elements +Partial ICE (at least V): 0 +Other partial element (R+C, R+V, V+C with distance between consecutive SPs > 14 CDS): 0 +## Composite elements +Total nested elements (partial or complete): 0 +Host element: 0 +Guest element: 0 +Element that are both host and guest: 0 + +##### Signature proteins ##### +## Total SPs detected +Total SPs: 4 +Total Integrase: 1 +Total Relaxase: 1 +Total Coupling protein: 1 +Total VirB4: 1 +## Unassigned SPs +Unassigned SPs: 0 +Unassigned Integrase: 0 +Unassigned Relaxase: 0 +Unassigned Coupling protein: 0 +Unassigned VirB4: 0 + +##### Segments ##### +Number of segments with one element: 1 +Number of segments with several elements: 0 +Number of segments with nested elements: 0 +Number of segments with exclusively isolated SPs to manually verify: 0 + +##################### ICEscreen statistics for genome accession NC_020450.1 ##################### + +##### Mobile elements ##### +## Number of complete elements +Complete ICE (4 types of SP): 1 +Complete IME (R+I, R+C+I with distance between consecutive SPs <= 14 CDSs): 0 +## Number of complete modules +Conjugation module (R+C+V): 0 +Mobilizable element (R+C with distance between consecutive SPs <= 14 CDS): 0 +## Number of partial elements +Partial ICE (at least V): 0 +Other partial element (R+C, R+V, V+C with distance between consecutive SPs > 14 CDS): 0 +## Composite elements +Total nested elements (partial or complete): 0 +Host element: 0 +Guest element: 0 +Element that are both host and guest: 0 + +##### Signature proteins ##### +## Total SPs detected +Total SPs: 4 +Total Integrase: 1 +Total Relaxase: 1 +Total Coupling protein: 1 +Total VirB4: 1 +## Unassigned SPs +Unassigned SPs: 0 +Unassigned Integrase: 0 +Unassigned Relaxase: 0 +Unassigned Coupling protein: 0 +Unassigned VirB4: 0 + +##### Segments ##### +Number of segments with one element: 1 +Number of segments with several elements: 0 +Number of segments with nested elements: 0 +Number of segments with exclusively isolated SPs to manually verify: 0 +
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/NC_013798_298468_322494_NC_020450_643089_661957_detected_ME.tsv Tue Feb 28 14:10:38 2023 +0000 @@ -0,0 +1,3 @@ +ICE_IME_id Segment_number Genome_accession Category_of_element Category_of_integrase Host_ICE_IME_ids Guest_ICE_IME_ids Colocalized_ICE_IME_ids ICEline_format ICE_consensus_superfamily_SP_conj_module ICE_consensus_family_SP_conj_module IME_relaxase_family_domains_blast HMM_family_SP_conj_module Integrase_upstream Integrase_downstream Relaxase Coupling_protein VirB4 List_SP_ordered_genomic_position Other_potential_SP_conj_module_need_manual_curation_and_review Other_potential_integrase_need_manual_curation_and_review Comments_regarding_structure +ID_1 1 NC_013798.1 Complete ICE (V+C+R+D) one integrase DDE - - - V8.C7.R2.D Tn5252 TnGBS2 - C:VirD4, R:MOBP, V:VirB4 - GALLO_RS01755 GALLO_RS01740 GALLO_RS01695 GALLO_RS01650 GALLO_RS01650, GALLO_RS01695, GALLO_RS01740, GALLO_RS01755 - - Downstream integrase GALLO_RS01755 has been associated to the structure ID_1 pre-merging because it is adjacent to the conj module and there is no upstream/downstream ambiguity. +ID_2 2 NC_020450.1 Complete ICE (C+R+V+I) one integrase Tyr - - - C1.R1:V5.I Tn916 - - C:TcpA, R:MOBT, V:VirB4 - LILO_RS03350 LILO_RS03260 LILO_RS03250 LILO_RS03320 LILO_RS03250, LILO_RS03260, LILO_RS03320, LILO_RS03350 - - Downstream integrase LILO_RS03350 has been associated to the structure ID_2 pre-merging because it is adjacent to the conj module and there is no upstream/downstream ambiguity.
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/genbank/NC_013798_298468_322494_NC_020450_643089_661957.gb Tue Feb 28 14:10:38 2023 +0000 @@ -0,0 +1,1732 @@ +LOCUS NC_013798 24027 bp DNA linear CON 24-DEC-2022 +DEFINITION Streptococcus gallolyticus UCN34, complete sequence. +ACCESSION NC_013798 REGION: 298468..322494 +VERSION NC_013798.1 +DBLINK BioProject: PRJNA224116 + BioSample: SAMEA2272396 + Assembly: GCF_000027185.1 +KEYWORDS RefSeq; complete genome. +SOURCE Streptococcus gallolyticus UCN34 + ORGANISM Streptococcus gallolyticus UCN34 + Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae; + Streptococcus. +REFERENCE 1 + AUTHORS Rusniok,C., Couve,E., Da Cunha,V., El Gana,R., Zidane,N., + Bouchier,C., Poyart,C., Leclercq,R., Trieu-Cuot,P. and Glaser,P. + TITLE Genome sequence of Streptococcus gallolyticus: insights into its + adaptation to the bovine rumen and its ability to cause + endocarditis + JOURNAL J Bacteriol 192 (8), 2266-2276 (2010) + PUBMED 20139183 +REFERENCE 2 (bases 1 to 24027) + AUTHORS Rusniok,C. + TITLE Direct Submission + JOURNAL Submitted (23-NOV-2009) Rusniok C., Unite de Biologie des Bacteries + Intracellulaires, Institut Pasteur, 25, 28 rue du Docteur Roux, + 75015, FRANCE +COMMENT REFSEQ INFORMATION: The reference sequence is identical to + FN597254.1. + The annotation was added by the NCBI Prokaryotic Genome Annotation + Pipeline (PGAP). Information about PGAP can be found here: + https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ + + ##Genome-Annotation-Data-START## + Annotation Provider :: NCBI RefSeq + Annotation Date :: 12/24/2022 01:57:52 + Annotation Pipeline :: NCBI Prokaryotic Genome + Annotation Pipeline (PGAP) + Annotation Method :: Best-placed reference protein + set; GeneMarkS-2+ + Annotation Software revision :: 6.4 + Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA + Genes (total) :: 2,343 + CDSs (total) :: 2,250 + Genes (coding) :: 2,234 + CDSs (with protein) :: 2,234 + Genes (RNA) :: 93 + rRNAs :: 6, 6, 6 (5S, 16S, 23S) + complete rRNAs :: 6, 6, 6 (5S, 16S, 23S) + tRNAs :: 71 + ncRNAs :: 4 + Pseudo Genes (total) :: 16 + CDSs (without protein) :: 16 + Pseudo Genes (ambiguous residues) :: 0 of 16 + Pseudo Genes (frameshifted) :: 11 of 16 + Pseudo Genes (incomplete) :: 6 of 16 + Pseudo Genes (internal stop) :: 5 of 16 + Pseudo Genes (multiple problems) :: 5 of 16 + CRISPR Arrays :: 2 + ##Genome-Annotation-Data-END## + COMPLETENESS: full length. +FEATURES Location/Qualifiers + source 1..24027 + /organism="Streptococcus gallolyticus UCN34" + /mol_type="genomic DNA" + /strain="UCN34" + /sub_species="gallolyticus" + /db_xref="taxon:637909" + gene <1..24 + /locus_tag="GALLO_RS01645" + /old_locus_tag="GALLO_0278" + CDS <1..24 + /locus_tag="GALLO_RS01645" + /old_locus_tag="GALLO_0278" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_001037290.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="PrgI family protein" + /protein_id="WP_012961395.1" + /translation="MNKLGSEFFKAIDQFERSIIGGFTWRQIIMMLGIVVGAGLAALI + TMFKLPSILFYLSLTLTIVPSFVYGIKKDEVIKERLLFKFKIQERSYQTEYESEEING + KYIPEKGVHEWNDLD" + gene 2..2362 + /locus_tag="GALLO_RS01650" + /old_locus_tag="GALLO_0279" + CDS 2..2362 + /locus_tag="GALLO_RS01650" + /old_locus_tag="GALLO_0279" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_001014604.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="ATP-binding protein" + /protein_id="WP_044555479.1" + /translation="MSGTTLTKEDKERAKQLKKALKASTQNTIKYTSLFEDGLMHIDG + EEYSKTWELGDANYLTADEEEKLDIIDYYVEALNGLDSDNTYQLLIINRPVPSTLLSQ + ITYEVEGDNRDIFRQEYNDMITARFATDQNNFKVEKFVTVSTQSRDRKQAYRKLNDVD + NHFKKQFQVVDIPFKSLNGTDRLNIFADLLRGNPYLNVDYKDVRISDLTTKSFIAPGR + ILFQEDQFLMDKQYCRVLFARSFPAFLNDRLVKSITDIGIELAITIHAKPYDVAEAVK + KVNTAEAGVKMDMVKSQAAAAEKGISSNLAVSSVAQATAEEAEKWKTEINDNDQKMFS + GVFAVMLKADTPEELADYTSRVKQAGRKHVVEFEEIYYHQEEALNTMLPIGKTYLDVK + RRFMRDLTTTNIATQIPFTNTDLQSNSPLANYYGQNQISNNIITLDRQRDLATASGVI + LGSSGSGKSVFVKTNEVIPAILRFPNDRVIIVDPEEEYVDIGRAFDAQVIDIYPGTKT + HFNLMDIPDQDKLRDEDKDFVGQKSSLIMGLFENILQEVTDDDISLIDRVTRLCYEQI + TDRTPTLKDWHDILLEQPEEEAQSLALKSESYTKGSQDIFAYETNVDLNKQVVIFNLK + KLSGKLKPFALMVIQDYIWQHVVEHKGEFVTRAYFDEMQYQFETEDQATFFTNMYARI + RKYGSIPTGITQSVETLLDRKEGRNLLYNSEFIVLLKQKKTTIPYLLKTINLTDALIR + YVEKPKAKGTGLIIAGEIAVPFENPIPENTELFRLVATDAYRKIEE" + gene 2364..2957 + /locus_tag="GALLO_RS01655" + /old_locus_tag="GALLO_0280" + CDS 2364..2957 + /locus_tag="GALLO_RS01655" + /old_locus_tag="GALLO_0280" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_017794877.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_012961397.1" + /translation="MFEDVTLKLSKKKEKELLAHYNTKIQDAIEKTFEDKEFYKPMVR + MSGLCRWLDVSTTTVVKWQKEGMPHMVIDGVTLYDKRKILKWLNHFERNKIISQENQM + KVLPGYLSIKHVFGTIITYPKEVVLDLIEDLRETDVQDFSYWSPLYFLEYDELGCPVY + LTYLVNEVGIAMHPNRYYYVTLTEEQEKALESLELSI" + gene 2972..3220 + /locus_tag="GALLO_RS01660" + /old_locus_tag="GALLO_0281" + CDS 2972..3220 + /locus_tag="GALLO_RS01660" + /old_locus_tag="GALLO_0281" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_017647992.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_012961398.1" + /translation="MNLKDFETIFQQQTKARNKRILIVLGIFFSVITAKHIFGLIITS + ILSLAFIVDFVWLRYKKRKKWLETDTKAIAAKRRVESE" + gene 3259..5958 + /locus_tag="GALLO_RS01665" + /old_locus_tag="GALLO_0282" + CDS 3259..5958 + /locus_tag="GALLO_RS01665" + /old_locus_tag="GALLO_0282" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_000066069.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="phage tail tip lysozyme" + /protein_id="WP_012961399.1" + /translation="MSDKNDLRKRVVRNRHIKESVQRHNQLSARKAQKAAKAEYQKAK + TNLKKAEEALNKAKEELKTADLSDKEKAEKLKELEANVKKAKAEVKAAKKVKKKVRKT + NPTVGQRARKTGRNIGKTVSREVLTTALTQDDTLSDAVKLKRQARQTAITMKHAGRAT + AKGTKLAYRGAKGTVKLSVKATKYTYKGARKGTQKATQATAKVAAKTAQGFVTVAKSA + ASAATTLLANPITWIVSGVVALVLFLVIIVSSIFSSNVAQQTEFSLNQSWLHISQVDR + KNSSDQVDYYTDIDSILLYMNYRYGGDWEPDAKWDDGTGGKLAGTLGFNHFSDALDDI + WKEENKDIGNLKTMAELYTNGKEWLKLDDDDLAEYKEILESQSETGKYLAYQELSNPF + YAADDDKSETEYLTISKRYGYTDKDTIDPTSTLQASSGQKLYATMDGKVTVTTKDLKG + NETETTNVIITDSDARFIYYNIASIRVKTGDKVEAGTELGIVSGSSQKIAYVKKYGEV + DPKDSQWLQDIKDKDVSYGYTEKTTKNKTNVWVFVNPGFYFPFVKYSQTTVVTKTSSE + MSGRAKQFYDLVKKYYPNATDNGIAAVVGNFAVESSITAKRAEGDYLSPPVGASDGCW + DDETWLNMGGTEIYGGGYPNILHRGLGLGQWTDTSDGGTRHTMLINYANAIGKKWYEL + ETQVRFMLEGDSPYYQNILKSVLTSNADTDSLTKSFLTKWEGINNGTLSQRQSYAKQA + LSWFHQSIAGGGTLASSWDFPEEYRDKIKSMPTTASMTTQAGSGYKVGQCTWYAYNRL + VELGEITDLSGSYGHLGNGQDWVRNLVAKGWHYSTTPSVGAVCSTAGGFDSTYAIYGH + VMIVEAVNDDGSFLVSECNYAGNQSQIHWRVCQNASYYTFATP" + gene 5975..6592 + /locus_tag="GALLO_RS01670" + /old_locus_tag="GALLO_0284" + /pseudo + CDS 5975..6592 + /locus_tag="GALLO_RS01670" + /old_locus_tag="GALLO_0284" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_017794862.1" + /note="internal stop; Derived by automated computational + analysis using gene prediction method: Protein Homology." + /pseudo + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + gene 6576..6788 + /locus_tag="GALLO_RS11895" + /old_locus_tag="GALLO_0285" + CDS 6576..6788 + /locus_tag="GALLO_RS11895" + /old_locus_tag="GALLO_0285" + /inference="COORDINATES: protein motif:HMM:NF012651.2" + /GO_function="GO:0003697 - single-stranded DNA binding + [Evidence IEA]" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="single-stranded DNA-binding protein" + /protein_id="WP_012961400.1" + /translation="MSLTAKGDTLQVTGRLQTRTYKDKKEVTHKVTELQVDKWYLVGV + SQTQGDYPVKEKTIIKDSDNLDKLSL" + gene 6807..7082 + /locus_tag="GALLO_RS01680" + /old_locus_tag="GALLO_0286" + CDS 6807..7082 + /locus_tag="GALLO_RS01680" + /old_locus_tag="GALLO_0286" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_014295369.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_012961401.1" + /translation="MTEETYTTEQQTSKASRRQKSTSERLMAKQNQLAKAKASQAVMQ + KKIKGLEEEIKILENKRQQEILQEYGMSLSDLEAFLANNKDKLGGDA" + gene 7082..7912 + /locus_tag="GALLO_RS01685" + /old_locus_tag="GALLO_0287" + CDS 7082..7912 + /locus_tag="GALLO_RS01685" + /old_locus_tag="GALLO_0287" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_003030379.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_012961402.1" + /translation="MFRIQNLGTGQEQEFPNRAALLLGLEGEENRCLQLNQIATFQVF + HLDKNQEVLESMELTIPSSGSEDVKMLLGDFGLKKEESRAFWKRGRQKPKQEEQSKDR + SHPVKKVIVKEKAPKPSNLSKSQTASTLFGRGLKALVLVITLLLSCVSLWVSLSAREE + KQKSTAKTVQVTIDQKADVFCRYFIGNYFANSSAREDFVSKSLDLDQMTVEKATTLSV + LLESQTAQQTTTTLTYVISCRYDDETTENKRLTLTVKQNKEAKYGYLVTKLPQLTAYP + " + gene 7931..8425 + /locus_tag="GALLO_RS01690" + /old_locus_tag="GALLO_0288" + CDS 7931..8425 + /locus_tag="GALLO_RS01690" + /old_locus_tag="GALLO_0288" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_000371894.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="DUF3801 domain-containing protein" + /protein_id="WP_012961403.1" + /translation="MEQEQILDRGARVTLDTGRQLFQFLAYAAGQLYKVYDERKLTGK + QSYKNFFNQNSLTKDHIDFLEADVNLKKFTKELEKSGVRFAFKDNSDGTKQVWFEAHN + REVIADALRQTLNEIINDPKKAKEKYMKSEKELTPKEQITKIKEATKEKVDTVKAKKK + GKSI" + gene 8425..10455 + /locus_tag="GALLO_RS01695" + /old_locus_tag="GALLO_0289" + CDS 8425..10455 + /locus_tag="GALLO_RS01695" + /old_locus_tag="GALLO_0289" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_013851992.1" + /GO_component="GO:0016020 - membrane [Evidence IEA]" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="type IV secretory system conjugative DNA + transfer family protein" + /protein_id="WP_012961404.1" + /translation="MTYQKKPFIPYLLLSFVLAFCTHRAYVLYSLAPAPDMSDLFGQY + TYVLDHYFDRPIFYLDTSPLALLAALVGFFIGMLFYLKIKPGGTYRHGEEAGSARFAT + AQELSGFKDSEPTNNMIFSKQAQMGLFNKLLPFQWQLNKNVVVVGLPGDGKTFTYVKP + NLMQMNSSFIVTDPKGLLVREVGSMLEKHGYQVKVFDLVNLTNSDMFNPFHYMTSELD + IDRITEAIVEGTKKGDREGEDFWNQAKLLLNRALIGYLYFDSQVRDYTPNLSMVSELL + RNMKRPNEKEPSPVEKMFDELEAAMPGNYACRQWDLFNSNFEAETRTSVLAIVATQYS + IFDHEAVTDLIKADTMEMETWNTEKTAVFVAISETNKAYSFLASTLFTVMFDQLTHTA + DAIIQGQKEGYTTDELIHVQFIFDEFANIGKIPHFNEVLASVRSREMSVKIIIQAISQ + LDTIYGDKARKSIINNCATLLFLGTNDEDTMRYFSMRAGKQTITQNSYSEQRGQRVSG + TTSIQSHQRDLMTPDEIARIGVDEALVFISKQNVFRDKKAMVSDHPMKDELSNHPTDG + KWYAYRRFMVEDPEFFNAVYQGKISAENIWYPDMADYGEFVKENPFVESSQQATEGLV + DEQVPVQMAETTSESQSQTPPTHLRTVDLETGELFELPPEEEDETDDYYGEV" + gene 10865..11608 + /locus_tag="GALLO_RS01700" + /old_locus_tag="GALLO_0290" + CDS 10865..11608 + /locus_tag="GALLO_RS01700" + /old_locus_tag="GALLO_0290" + /inference="COORDINATES: ab initio + prediction:GeneMarkS-2+" + /note="Derived by automated computational analysis using + gene prediction method: GeneMarkS-2+." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_012961405.1" + /translation="MYDYFDFYGLWIEQLTILQKEGLSQWIENDDFFLFDFLYDLDGI + SFDCGNHYNCSLKFELNDKCYRNSQCHGIVLPLPTQPQLMNTAYSSVDDIVRDLKAAY + GQYLPKDFDYKANICHAQASMWEFGPDNDFKKNDEPIKQELPMFVIDGYHAISSKIGL + MIEYPEKILKDIFDLTEEDLSKSTRLSPLYEVSDSSKEKIYQYFLVDNMGLPLSNDSI + YWIKLSDKDDHLLKKVLIDLTSWISFKYS" + gene 11685..12038 + /locus_tag="GALLO_RS01705" + /old_locus_tag="GALLO_0291" + CDS 11685..12038 + /locus_tag="GALLO_RS01705" + /old_locus_tag="GALLO_0291" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_006739176.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_012961406.1" + /translation="MTIYELDPNFFVNLGKTAPYKVTEIIGKDHPDNFLTPGEEKSFL + ILDKEKAWIVHADSLVSVAEELEGYLGMSVDDFIMISLGTQYHKRFCFNDDYEIWERY + FTKLREAYYSDSSSK" + gene 12092..12574 + /locus_tag="GALLO_RS01710" + /old_locus_tag="GALLO_0292" + CDS 12092..12574 + /locus_tag="GALLO_RS01710" + /old_locus_tag="GALLO_0292" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_001202059.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_012961407.1" + /translation="MTKVLKKLIEENLSPLGIVELIDERYDKFGGYYLLYSLSLTAIT + PEFSESGTVDIEVSFSDYQELTYELGKYSIPFYVNSKEELKCLLEGIVDLPLPFSVSY + EPIKEVTWDRYIVSIDGVKTTRIIELVTMIKVLEDYLSQIEPLLNQNVLKIMKNRENN + " + gene 12609..14345 + /locus_tag="GALLO_RS01715" + /old_locus_tag="GALLO_0293" + CDS 12609..14345 + /locus_tag="GALLO_RS01715" + /old_locus_tag="GALLO_0293" + /EC_number="5.6.2.-" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_017768605.1" + /GO_function="GO:0003677 - DNA binding [Evidence IEA]; + GO:0003916 - DNA topoisomerase activity [Evidence IEA]" + /GO_process="GO:0006265 - DNA topological change [Evidence + IEA]" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="DNA topoisomerase" + /protein_id="WP_052828722.1" + /translation="MKYLLLAEKPDQAKKYATALGNPKNEKGVWRVQSSVLNAEVIVA + PAVGHLVERINPYTNFENWEMTNLPALPEGFSYEPKKDTIKRFNAIKKAVKEVDAIII + GTDPDREGEAIAYRILELIPEALRKIKYRLWANSLTKRGLEQAFANLRSPTDSINYFH + EADARSDADWLVGFNLSPFVTIKMKEEGYLDKKDHSMSVGRVQTPIVSLIVRNDEAIE + NFKPSPYWRLKLIDSEAEVIFSNDIKYQSQMEAEQVLQQLANTAVVKTVTSEEKAQEA + PKLYNLTNLQSEMSKLYHFDATKTKELVQSLYQKGYLSYPRTDSTLITTNEFSYLVDH + IEEYQKAINKQLETPNRSPRKNYVNDKKVLEHYAIIPTENIPDLSELSDDEKLIYQKA + VFRTLLMFTPDYRYQSTSVILDNHGLEFKATGTVTKDKGWRGYFAVKKEDKELPHYQE + GQEVIVDCNLLEEMTKPPTRITEQILLKKLLPKYHLGTSATRDGMIDLIQDKGYVTKH + KKTGQFFPTERGKLLIHYLDQLEIAYTNPETTGKWEEVLAQIGQGKIQKEAFVNKIKW + AITKQIEKGKQL" + gene 14398..18747 + /locus_tag="GALLO_RS01720" + /old_locus_tag="GALLO_0294" + CDS 14398..18747 + /locus_tag="GALLO_RS01720" + /old_locus_tag="GALLO_0294" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_000002618.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="PBECR4 domain-containing protein" + /protein_id="WP_012961409.1" + /translation="MANMQELLSQRFEAQRQKLVSMDIVAVAESLGMTLKPGSSGTYY + WEEHDSFHIYPNTNTFRWWSRSIGTNTIDLVQVVQEEMTGHKPSFREAAAFLETGHFE + NVTVQPIVKEPFEYYLERYEHPDFNIGRQYLKEERGLSDETIDTFLASGNMATATRKK + GDYFEPVIVFKSCDNDGKMIGASLQGLVENRVQHPERGRLKQIMKNSEGTAGFSLDVG + TPKRLVFAEAPIDLMSYYEVNKDDLQDVKLVAMDGLKKGVISRYVADMLTDGKYSQTM + SAEQIRGALDALNQTTNLLQEHSDMITLAVDNDEAGQNFVKGLQDDGIPVIPDLPPRQ + PAQKKMDWNDYLKQMKKESAQMAETPEVEKQSLSELQESLSNEGDLVSTITSDSSIIY + TNTVSFNQDYNLNLEIHSPNEVDNLSDIQAPWTLEVMKKGQSLGYLAYGEDWGNDFDI + EDELVNLENWVKDNQVTNHLYTQKDINAFLASTQEVEPKMGDFVLKGGGIYDFGKSQL + EGEELRQHAIDAMISDITDAETYYLWHDEELEKLNAPDEAFLNFHYHLQDIQYNQNDI + HLYVSESSTDGVTGYLSLDGNSLDSDSIEEYLADQDWTFNQKVQFLKNLKTAVDDTWN + KVTDHYNEQFNAIVDQYGLSLQKEKTPEVPQEQESANSEQQKKVENPIGDLPEQSQEA + APLPNTHEEYSLNESSPTQTQSQPLLHFNISNPDKSIYKVGYHPVNRKELNKLNRYAA + QIQENATWYKNELADSNVTYFYKNNDNLEALKVTFKAEQYPHLIGFFAIDENQSASKT + LDDLASGKGDYQNIMVANRGATFSKIQVLPDFKAVVDSNSFIFDDLSEVERMNRLDLA + KAIRTEDKDVLIAFRNVDGEYLPASLMKVTNKLESELSIANNKDVLGIVGEKNGEFKV + LSVNEEIIKDGGKKMLSIVKNNQFENLSKAEVTAEDFTKVLDAVYNVGAQVGKDNRAN + IPKELYPAWDKYYEYAERYDNNFDVIVKAARADNLFDENSDFYKDVWLRKIQEENQKV + KDSDGDGLTDDEEIALGTNPYSPDTDGDSVIDSIERGSGTDATNPSDTPDNRQKEQMK + HNLTLSEMIKAKDTVALNQHLQEGIKDYFDSDTYKNYLEGMAHFNHYSARNIQLIKAQ + LPEATMVASFEEWKKRNGHVNKGEKALYVQAPVTVIKKDADGNPVINSETGEKETFTY + FKPVPVFDISQISPQQGKQLNLPKSSEAIPAQLNKDYYQNVYRALRDISQKENGIPIR + FRELEQFDGFYNPKTNDITIKKGMTYEQTLSTLIHEMAHSELHNKKSLTERFEGKLTR + SSKELQAESVAYVVSNHLGFDTSNDSFAYLASWSQEPDGLENLKAQLEIVQEEASSLM + NRIDKQLTKYQTLTVSKDETLTETQKRNLEKSANPFYQSLQKAKAEVNNMKKGDEPNN + EQTVKKDNRPTKC" + gene 18796..19089 + /locus_tag="GALLO_RS01725" + /old_locus_tag="GALLO_0295" + CDS 18796..19089 + /locus_tag="GALLO_RS01725" + /old_locus_tag="GALLO_0295" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_000255675.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_012961410.1" + /translation="MITDEMMTITEMILMSYYFDMSEWLKGNKIINSDIVQKSKDDLL + DVLKTDFEQLTTEDNNDYLDDLSISIGTLEELSEDNYQKLKTAIFSWEPSKKK" + gene 19402..19776 + /locus_tag="GALLO_RS01735" + /old_locus_tag="GALLO_0296" + CDS 19402..19776 + /locus_tag="GALLO_RS01735" + /old_locus_tag="GALLO_0296" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_000436063.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="plasmid mobilization relaxosome protein MobC" + /protein_id="WP_012961411.1" + /translation="MTIKRRKRPIEFKLRTTDEEAFQLRQRITMANKKTFQGYALEML + LKGKIETYDYSELQRLRVEVNHIGQNVNQLVRYVNTFDEIDSELFKALQAEIDEMKVL + LIDEFKTKGRAKLNGGDKSNPD" + gene 19748..21367 + /locus_tag="GALLO_RS01740" + /old_locus_tag="GALLO_0297" + CDS 19748..21367 + /locus_tag="GALLO_RS01740" + /old_locus_tag="GALLO_0297" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_008090066.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="relaxase/mobilization nuclease domain-containing + protein" + /protein_id="WP_012961412.1" + /translation="MVVTKVIQIKSSRNLKRAINYITRDNATLKLDSERLEGDDNYSY + EMVNGQVMKRLVSGHDVTDISDPQTIYDDFVLLKQSVDALYNNDELSDLKNDKRVLAH + HIIQSFSPEDGLTPEEVNEIGRKTALELTGGDYQFVVATHMDKGHLHNHIIFNTTNEV + TLKKFRWQKGTKKSLEHISDKYAELYGAKVLEPRLRNSHTDYSAWRRKNNFRFEIKER + LNFLLKHSLDMTDFLQKAKTLNLQVDTSGKYIKYKLLDQPQERFVRDRTLSKKGKFSL + EKIKEQIATNEVVYDLNVIKEKYDEEQESKQDDFEMQLTIEPWQIEQLTHQSIYVPIT + FGLDRKGTVSIPARMLDQNEDGTFTAYLKKNDFFYFLNTDHSEQNRFIKGVTLIKQLS + AQNGEMILTKNKNITNLDRLVDEFNFLAANNVTNSKQFENLQQQFLEQLDETDKTLAA + LGDKIFYLNKLLGALSDYQNNIVPSEVSLDLLEKGKIDKTMKLDELQKEIKELQIERD + TLKKHRDKIVKDYDFTKEIKQTHEKRTGISL" + gene 21472..21660 + /locus_tag="GALLO_RS01745" + /old_locus_tag="GALLO_0298" + CDS 21472..21660 + /locus_tag="GALLO_RS01745" + /old_locus_tag="GALLO_0298" + /inference="COORDINATES: ab initio + prediction:GeneMarkS-2+" + /note="Derived by automated computational analysis using + gene prediction method: GeneMarkS-2+." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_012961413.1" + /translation="MATNKEQIKNSGKCLFVETTTKLSSDELLKAIADDNMTEEQVEQ + LKIDLDIFLKQRAAAFKE" + gene 21688..22395 + /locus_tag="GALLO_RS01750" + /old_locus_tag="GALLO_0299" + CDS 21688..22395 + /locus_tag="GALLO_RS01750" + /old_locus_tag="GALLO_0299" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_013643517.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="class A sortase" + /protein_id="WP_148211633.1" + /translation="MKLLKGLLLVLLLLIGVALIFNKSIRNTLIAWNTNQYQVSQVSK + QTIEQNKDANVSYDFDSVESISTESVLKAQTNSANLPVIGGVAIPEVGINLPIFKGLG + NTELTYGAGTMKENQVMGSGNYALASHHVFGLTGSSQMLFSPLENVKTGMKIYLTDKE + TVYTYIVTSIESVAPSQVAVIDDTEETEVTLVTCTDAEATSRTIVKGVYDSEVSFDQA + PEDILDAFNTAYNQIAL" + gene 22686..24026 + /locus_tag="GALLO_RS01755" + /old_locus_tag="GALLO_0300" + CDS 22686..24026 + /locus_tag="GALLO_RS01755" + /old_locus_tag="GALLO_0300" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_006531843.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="UPF0236 family protein" + /protein_id="WP_044555481.1" + /translation="MVVMRFDERGFVEELYRQREYEFLKRVAAYDKQIAPTMRANGYK + RVDSSERTVLFTFGAMTFSRSRWRRGTKTRYPVDEWLGLKKYMRCSLELMYHLVRYAS + EMSYRQVCRMIKLSYHLDITKDTVLKAVKLTGQLFSEKSHYRYFVEEEVSEKIKAPVI + YIEGDGVLVKTNTGGDEKHNTDLAHFLVHTGTKIVHGRTVLLNKHEIIHTDYEVARDE + LLDYLYNHFEITNQTILVTNSDNGKGYTRRVFQNIQKALKIKRHEHFWDAHHVKEKIT + SFFKSYPKILKDLMFKAIQTHNRKLLKTVLDTVESLVTDDEEYLVFQKFRKKFLNNFK + DTKPAKLRGLSHKGIGVMESQHCKVTFRMKHRGMYWSIKGACAMARLILLERIDQLEN + LFFGNWRKDYQYYKDNQLGVGYLRESKADHSPTPQRIFKRAGKIMSLDRQKFKY" +ORIGIN + 1 catgagtgga acgaccttga ctaaagaaga caaagagcgg gcaaagcagc tcaaaaaagc + 61 gttaaaggct agcacccaaa atacgattaa atacacctct ttatttgaag atgggctcat + 121 gcatattgac ggggaagagt attctaaaac ttgggaactt ggtgatgcga attacctaac + 181 ggcggacgag gaggaaaaac ttgatattat cgactattat gttgaagctt tgaatggttt + 241 ggatagtgat aatacctatc agctgctgat tattaatcgt cctgtaccct caaccctttt + 301 aagtcaaatt acctatgagg tagaaggcga taatcgtgat atctttcgtc aagaatataa + 361 cgatatgatt accgctcgtt ttgcgacaga tcaaaataat tttaaggttg aaaaatttgt + 421 cacggtctct acacaatcgc gagatcgaaa gcaagcctac cgtaaattaa atgacgttga + 481 caatcacttt aagaaacaat ttcaagtcgt tgacatcccc tttaagtcat taaatggaac + 541 tgaccgctta aatatttttg ctgatttact tcgtgggaat ccttatctga acgttgatta + 601 taaagatgtc cgtattagtg atttaaccac aaaatccttt attgctcctg gacgaatctt + 661 gtttcaagag gatcaatttc tgatggataa acagtattgc agggttcttt ttgctagaag + 721 ttttcctgct tttttaaatg atcgcttagt caagtctatt acagatattg gaattgaact + 781 agccattacg attcatgcga agccttatga tgtcgcagaa gctgttaaaa aagtgaatac + 841 ggctgaagca ggtgtcaaaa tggatatggt taagtcacaa gctgcggcag cggagaaagg + 901 tatctcaagt aatctagctg tttcgagtgt ggctcaagct accgctgaag aagctgaaaa + 961 atggaaaaca gaaatcaatg acaatgacca gaaaatgttc tctggtgtct ttgcagtcat + 1021 gcttaaggcc gatacaccag aagaattagc tgattatacc agtcgtgtca aacaggcagg + 1081 ccgaaaacat gtggttgaat ttgaagaaat ctattatcat caagaagaag ccttaaatac + 1141 catgttaccg attggtaaaa cgtaccttga tgtcaaacgt cgctttatgc gtgatttgac + 1201 cacaacaaat attgcaactc aaatcccatt taccaacacg gacttacaaa gtaacagtcc + 1261 attagctaat tattacggtc agaatcaaat ttcgaataac attatcactc ttgaccgcca + 1321 acgtgattta gcaaccgctt caggagttat tctagggtca tctggctcag gaaagtctgt + 1381 ctttgttaaa acaaatgaag tcattccagc gattttaaga tttcctaatg atcgtgtcat + 1441 catcgttgac cctgaagaag aatatgtcga tatcggtcgt gcctttgatg ctcaagtcat + 1501 tgatatctat ccagggacta aaacgcattt taatctcatg gatattcctg atcaagataa + 1561 actacgcgat gaagacaaag actttgtcgg tcaaaaatca tctctcatca tgggcttatt + 1621 tgaaaatatt cttcaagagg tgaccgacga tgatatctct ctgattgacc gtgtgactcg + 1681 tctttgttat gaacagatta ctgatcgaac accaacccta aaggattggc atgatattct + 1741 tctagagcag cctgaagagg aagctcaaag tcttgcgctt aaatcagaat cgtacaccaa + 1801 aggctcacag gatatttttg cttatgaaac gaatgtcgat ttaaataagc aagttgttat + 1861 tttcaacttg aaaaaattaa gtggtaaatt aaaacctttt gcactgatgg tcattcagga + 1921 ttatatttgg caacatgtgg tagaacacaa aggtgagttc gtcactagag catactttga + 1981 tgagatgcag tatcaatttg aaactgaaga ccaagctact ttctttacga atatgtatgc + 2041 tcgtatccgt aaatatggct ctattccaac aggtatcacc caaagcgttg aaactctact + 2101 agatcgtaaa gaaggtcgaa acttgctcta taatagtgaa tttattgttc tcttaaaaca + 2161 aaagaaaaca acgatacctt atctactaaa gacgatcaat cttaccgacg cccttattcg + 2221 ctatgttgaa aagccaaaag cgaaaggaac tggcttaatt attgcgggtg agatagcagt + 2281 accatttgag aatccaattc ctgagaacac tgaattgttc aggttggttg ctaccgatgc + 2341 ttatagaaag atagaggaat aaaatgtttg aagatgttac tttaaagtta tctaaaaaaa + 2401 aggaaaaaga gttattagct cattataata ctaaaataca ggatgctatt gaaaaaacct + 2461 ttgaagacaa ggaattttat aagccaatgg ttcgcatgtc tggtttatgt cgttggctag + 2521 atgtttcgac aactactgtt gttaaatggc aaaaagaagg tatgccccat atggtcattg + 2581 atggagtaac tttatacgat aaacgaaaaa ttttaaaatg gttaaatcat tttgaaagaa + 2641 ataaaattat ttctcaagaa aatcagatga aagttttacc tggctactta tcaattaagc + 2701 atgtatttgg gacaattatt acttatccta aggaagttgt tcttgattta attgaagatc + 2761 ttagagaaac tgatgttcaa gatttttctt attggtcacc actatatttt ttagaatatg + 2821 atgaactagg atgccctgtt tatttaactt atctagtaaa tgaagttggt attgcgatgc + 2881 atccaaatcg ttattactac gttacattga cagaggaaca agagaaggca ctggaaagtt + 2941 tagaattatc tatttaaaaa gaggtattgt tatgaactta aaagattttg agactatttt + 3001 tcaacaacaa actaaagcta gaaataaaag aattttgata gttttaggta tcttttttag + 3061 tgtcataacc gctaagcata tatttggttt aattattact agcatattgt cactggcatt + 3121 catagttgat tttgtttggc tacgctataa gaagcgtaag aaatggctgg aaacagatac + 3181 taaagcaata gcagcaaaac gtagagttga aagtgaatag aaatgcttaa aagaaatata + 3241 cctgaaaggg ggtgaaccat gtcagataaa aatgatttaa gaaagcgtgt ggtgagaaat + 3301 cgtcacatta aagaaagtgt tcaacgtcac aatcagctat ctgctcgaaa agcccaaaag + 3361 gcagcgaaag cagagtatca aaaagcaaaa acaaatctca aaaaagctga ggaagcttta + 3421 aataaagcta aagaagagtt aaaaacggct gatttgtcag ataaagaaaa agctgaaaag + 3481 ctcaaagagt tagaggctaa tgtcaaaaaa gctaaagctg aagtgaaggc tgctaagaag + 3541 gtcaagaaga aggttcggaa aacaaatcct acagttggtc aaagagctcg aaaaacaggt + 3601 agaaatattg gtaaaacggt tagtcgtgaa gtgttaacaa ccgctctgac acaagatgat + 3661 actctttcgg atgccgttaa gttaaaacgt caagcacgcc aaaccgcaat cacaatgaaa + 3721 cacgcaggcc gagcgactgc taaaggaacg aaattagctt atcgtggtgc caaaggaacg + 3781 gttaagttgt ctgtcaaagc taccaaatac acttacaaag gggctaggaa aggcacgcaa + 3841 aaagcgacgc aagctaccgc aaaagtagcc gctaaaacgg ctcaggggtt tgtgacagtt + 3901 gctaaaagtg cggcaagtgc cgcaacgact ttgctggcaa acccaattac ttggattgtt + 3961 agtggcgttg tagccctggt gcttttttta gttattatcg tttcctcaat cttttcaagt + 4021 aacgttgccc aacaaaccga atttagccta aatcagtcgt ggcttcacat ctctcaagtg + 4081 gatcgaaaga actcttctga ccaagtagac tactacaccg acattgattc cattttactt + 4141 tacatgaact accgttatgg tggtgactgg gaacctgatg caaaatggga tgatggtaca + 4201 ggtggaaaac tagcaggtac tcttggcttc aatcactttt cagatgcttt agatgatatt + 4261 tggaaagaag aaaataagga tattggtaac ctaaaaacca tggcggaact ttacactaat + 4321 ggtaaggagt ggctgaaact agatgacgat gatttagctg aatataagga aattcttgaa + 4381 agtcaatctg aaacaggaaa atatttagct tatcaagaat taagcaatcc tttttacgca + 4441 gctgatgatg acaaaagtga aacggagtat ttaacgattt caaagcgtta tgggtacacg + 4501 gataaggata ctattgatcc tacaagtacc ttacaagctt catcaggaca aaaactttat + 4561 gccactatgg atggaaaggt caccgtcaca acaaaagact taaaaggtaa tgaaacagag + 4621 acgactaatg tgattattac agatagcgat gctcggttta tctattataa tattgctagt + 4681 atccgagtca aaacaggtga taaagtcgaa gcaggaactg aacttggaat tgtttcaggt + 4741 agttctcaaa agatagccta tgttaaaaaa tatggtgagg ttgatcctaa agattctcag + 4801 tggttacaag acatcaagga caaagacgtt tcctatggct acaccgaaaa aacaacaaag + 4861 aataagacta atgtctgggt gttcgttaac ccaggctttt attttccctt tgtaaaatat + 4921 agccaaacca ctgtggtgac caaaacatct tctgaaatga gtggtcgagc gaaacagttt + 4981 tatgatttag tgaaaaaata ttacccaaat gcgacggata acggtattgc ggctgttgtt + 5041 ggtaactttg cggtggagtc ctcaattaca gcgaaacgtg ctgaaggcga ttatttaagt + 5101 ccgcctgttg gtgcatctga tggttgctgg gatgatgaaa cttggttaaa tatgggcggt + 5161 acagaaatct acggtggtgg ctaccctaat attcttcatc gaggacttgg cttaggtcaa + 5221 tggacggaca cctctgacgg tgggactcgt cataccatgt taatcaatta tgcaaatgct + 5281 attggcaaaa aatggtatga attggaaacg caagtccgat tcatgttgga aggtgattca + 5341 ccttattatc aaaatatatt gaagagtgtt ttaacttcta atgctgatac agatagcctt + 5401 accaaaagtt tcttaacgaa atgggaaggg atcaacaatg gtacactttc tcaacggcaa + 5461 agctatgcca aacaagcctt atcttggttc caccaatcaa ttgcaggtgg tgggacatta + 5521 gcttcctctt gggattttcc tgaagaatac cgagataaaa tcaaaagcat gccaaccact + 5581 gcaagcatga ccacacaagc aggtagtggt tataaagtgg ggcaatgcac ctggtatgct + 5641 tataaccgtt tggtagaact cggcgaaatc acagacctat caggaagtta tggtcatcta + 5701 ggaaatggtc aggattgggt gaggaattta gttgcgaaag gttggcatta ttcaacgaca + 5761 ccatctgtag gagccgtttg ctcaaccgct ggtggttttg acagcactta cgctatttac + 5821 ggacatgtca tgattgtcga agcggttaat gatgatggta gcttcttagt ttcggaatgc + 5881 aattatgcag gaaatcaatc ccaaattcac tggcgagttt gtcaaaacgc ttcttactat + 5941 acatttgcga caccatagaa aggaaaagaa atcaatgata gatacgaata aactgacctg + 6001 gtataaacgt ctttttttag tgattggctt tattattgtt tttctcatcg gcttaggatt + 6061 cggtcaaatg gggaaaacga atcaaaaaca agtgacaaca cctaagacaa cgaccaaaaa + 6121 ggtaacagca ggtagttaat tgacggaaaa gtgggtcaaa aagttcttaa ttgcttacta + 6181 taccaaaaaa gatttggaag agaaccgcaa tcgttataaa gaatatatga ctgaaggcat + 6241 gtataatgcg acgatttctg aagaagaaac cgcccaaaac caagcctata aaggctatgt + 6301 ggttaatttt gaatttcagt cagcccaaat ttatatcgat caaaccaatg aaaaagtcat + 6361 ttgctacgtg aattacacga atgaccttct tcaggaaaaa gattccagtg aaggcgcaca + 6421 aacagatgtt agcaacacaa caattatcca actaacctac agtaaagtgg ataaaacgta + 6481 tcttgttaat aatatgagta ctctgttaat cacagattct caagacccaa ccagttcaca + 6541 agaagcttat ggcgatgtga ttgcgagtaa ttttggtgag cctaaccgct aaaggagata + 6601 ccttacaagt cacaggacgc ctacaaacac gaacctataa ggataaaaaa gaagtgactc + 6661 acaaagtgac agaacttcag gttgacaaat ggtaccttgt tggtgttagc caaactcaag + 6721 gtgattatcc tgttaaagaa aaaacgatta tcaaggatag tgataacttg gataagttat + 6781 cactttaaaa cgaaaggaaa cagaatatga ctgaagaaac atatacaact gagcaacaga + 6841 cttcaaaagc ttcacgtcgt caaaaatcaa cgagtgagcg tttaatggct aaacagaacc + 6901 agttagcaaa agcaaaagcc tcacaagctg ttatgcaaaa gaaaattaaa ggcttggaag + 6961 aagagattaa gattctcgaa aataaacggc aacaagaaat tttacaagaa tatgggatga + 7021 gcctttcgga tttggaagct tttctagcta ataacaaaga taaattaggg ggtgatgcgt + 7081 aatgtttcgc atccaaaatt taggaacagg acaagaacaa gaatttccaa accgagcagc + 7141 tcttttattg ggattggaag gtgaagaaaa tcggtgttta cagttaaacc aaattgcaac + 7201 ctttcaagtc tttcaccttg ataaaaatca agaagtatta gagtcaatgg aactgaccat + 7261 tccctcatct ggcagtgagg atgtcaaaat gcttttgggt gattttggac tcaaaaaaga + 7321 ggaaagcagg gccttttgga agcgtggtcg tcagaagcca aaacaagaag aacagtctaa + 7381 agatcgttct catccagtca aaaaggttat tgtaaaagaa aaagccccta agccatctaa + 7441 cttatcaaaa agtcaaacag ctagcacact ttttggaagg ggacttaagg cactcgtatt + 7501 agtgattact ttgttactgt cttgtgtgag cctgtgggtc tctttaagcg caagagaaga + 7561 aaaacaaaag tctacggcca aaacagtgca agtcacgatt gatcagaagg cagatgtgtt + 7621 ttgtcgttat tttattggta actattttgc caattcctca gctcgtgagg atttcgtgtc + 7681 gaaatcactg gatcttgacc aaatgacagt tgagaaagct actacgctta gtgtgttact + 7741 ggaaagccaa accgcacagc aaacgacaac aactttgacg tatgtgattt cgtgtcgcta + 7801 tgatgatgag acgaccgaaa ataaacgctt aacattaacg gttaaacaaa ataaggaagc + 7861 taaatatggt tatctagtga ctaaactacc acagttaacc gcttatcctt aagtaggagg + 7921 aaaaaacgtt atggaacaag aacaaatctt agatagaggc gcccgtgtga cgttagatac + 7981 gggaagacaa ctgtttcaat ttttagctta tgcagcaggt cagttatata aagtttatga + 8041 tgaacgcaaa ttaactggga agcagtccta taaaaacttt ttcaatcaga actcactgac + 8101 gaaagatcat atcgatttct tagaagcaga tgtcaacctt aaaaaattta ccaaagagct + 8161 cgaaaaatca ggggtacgtt ttgcttttaa agataatagt gatggcacga aacaggtttg + 8221 gtttgaagcg cacaatagag aagttatcgc tgatgcgctt cgccaaacgc ttaatgagat + 8281 tatcaatgat cctaaaaaag ctaaagaaaa atacatgaaa tctgaaaagg agttaacacc + 8341 aaaagaacag attaccaaaa tcaaggaagc cactaaggaa aaagtggata cggttaaagc + 8401 gaaaaagaaa ggaaagagta tttgatgacc tatcaaaaga aaccattcat tccttatctt + 8461 cttctcagtt ttgttttagc tttttgtaca cacagggcat atgtcttata tagtttagct + 8521 cctgcccctg acatgtctga tctatttggt caatatacct acgttttaga tcattatttt + 8581 gatcgaccaa ttttttatct tgatacgagt ccactagcgc ttttagcagc tttagtgggc + 8641 ttttttattg gcatgctatt ttatttgaaa attaaaccag gtggtactta tcgccatggt + 8701 gaagaagctg ggtcagctcg ctttgcgact gctcaagaac tttctggttt taaagattcc + 8761 gaaccaacca acaacatgat tttttcaaaa caagcccaga tggggctatt taataagtta + 8821 ttaccctttc aatggcaact gaataaaaat gtcgtcgtgg taggtcttcc tggcgatggg + 8881 aagaccttta cttatgtaaa gcctaatttg atgcaaatga attccagttt tatcgtaact + 8941 gaccctaaag gactcttagt tcgtgaggtt ggaagtatgc ttgaaaaaca tggctatcaa + 9001 gtaaaagtct ttgaccttgt caatctcacc aattcagata tgttcaatcc ttttcattac + 9061 atgacgtctg aactagatat tgatcgtatc acggaagcga ttgttgaagg cactaaaaaa + 9121 ggtgatcgtg aaggcgaaga cttttggaat caagcgaaac tacttcttaa tcgggcttta + 9181 attggttacc tctattttga tagtcaagtt agggattata cccctaactt gtcaatggtt + 9241 tctgagcttc ttcgaaacat gaaacgtcca aatgaaaaag aaccaagtcc agttgaaaag + 9301 atgtttgatg agcttgaagc agccatgcca ggaaattatg cttgtcggca atgggatctg + 9361 tttaacagta actttgaagc agaaacaagg acgagtgttc tagccattgt tgctacccaa + 9421 tactctatct ttgaccatga agctgtcaca gatttgatta aagctgatac gatggagatg + 9481 gaaacatgga acactgaaaa aacagcagtc tttgtggcta tttcagaaac gaataaagct + 9541 tatagctttt tagcctctac tttatttaca gttatgtttg accaactaac acacactgca + 9601 gatgcgatta tccaaggaca aaaagagggg tacacgactg atgagttaat acacgtgcaa + 9661 ttcatttttg atgagtttgc gaacattggt aaaattcctc attttaatga agtcttggct + 9721 tctgttcgta gtcgtgaaat gtcggtcaaa attatcattc aggccattag tcagctagac + 9781 acgatttatg gcgataaggc tcgaaaatcg attatcaaca actgtgcaac cttgttattt + 9841 ttagggacta acgatgaaga tactatgcgg tacttttcaa tgcgtgcagg aaaacaaacg + 9901 attacgcaaa acagttattc tgaacagcga gggcaacgtg tttcaggaac aacaagtatt + 9961 caatctcatc agcgggattt aatgacacct gatgagattg ctcgtattgg ggttgatgag + 10021 gctctggtct ttatttccaa acaaaatgtt tttcgggata aaaaagcaat ggttagcgac + 10081 catcctatga aagatgagtt atctaatcat ccaaccgatg gcaagtggta tgcttatcgt + 10141 cggttcatgg tagaagaccc agaattcttt aatgcggttt atcaagggaa aatctcagct + 10201 gagaatattt ggtatccaga tatggcagat tacggtgagt ttgtgaaaga aaatccgttt + 10261 gttgagagtt ctcagcaagc cacagagggt cttgttgatg aacaagtacc cgttcaaatg + 10321 gctgaaacga cctcagaaag ccaatctcag acaccaccaa ctcacttaag gacagtagat + 10381 cttgaaacag gtgaactctt tgaattacca cctgaagaag aggacgaaac tgatgattat + 10441 tatggtgaag tctaacgtca aatgagttat aataacttag ggaggttaac atgacaagag + 10501 aaaagcaaag caaagctctt tttttaaaat acttggcatt tcagaagacg acttattaag + 10561 ttatctgaag gaacatccta acgaccctct agttagtaaa ctagagcaat tattattaaa + 10621 cttatcagct aaaaaatagg ctacatcgtt aaacggtgta gcttttgttg tatgtatatg + 10681 gaggaaaaat agatggaaca aaaatattat gcggtactaa tgcaaatcaa tattggtaag + 10741 agcgtctggt tagtccctca agaaagtggg aaactagcct ggtcatttaa ttagaaatta + 10801 tttttatgct gcagttaatt ttagcaatta gctgtttttt agttacagaa aggattttat + 10861 tattatgtat gactatttcg atttttatgg cctttggata gaacagctta ctatcctaca + 10921 aaaagaagga ctgtctcaat ggatagaaaa cgatgacttc ttcctttttg actttttata + 10981 cgacttagat ggtatttcgt ttgactgtgg caatcattat aattgttctc tcaaatttga + 11041 actaaacgat aaatgttatc gtaacagtca atgtcatggg attgtattac cattaccaac + 11101 ccaacctcaa ctcatgaata ccgcttattc atccgtggac gatattgtac gagacttaaa + 11161 agcagcatat ggtcaatatt taccgaaaga ttttgattat aaagctaata tttgtcatgc + 11221 acaagcttcg atgtgggagt ttggtcctga taatgatttc aagaagaatg atgagcccat + 11281 taagcaagag ttaccaatgt ttgtaattga cgggtatcat gctatttcct caaaaattgg + 11341 tcttatgata gaatatccag aaaagatttt aaaggatatt tttgatttaa ctgaggaaga + 11401 cttaagcaag tcaacaagac tttctccatt atatgaagta agtgatagta gtaaagaaaa + 11461 gatataccag tattttttgg tagataacat gggtttacct ttaagcaatg atagtattta + 11521 ttggataaaa ttatcagata aagatgacca cttgttgaaa aaagttctga ttgatctgac + 11581 ttcatggatt tcatttaaat atagctagct gctcgttaat ttcagagagc tcactcatca + 11641 agaatgactt gattttgaaa taatcgtaaa ggtgaactaa aaagatgact atttatgaat + 11701 tagatcctaa tttttttgta aacttaggta aaacagcacc ttacaaggtg acagaaataa + 11761 tcgggaaaga tcacccagat aattttttaa ctcctggtga ggaaaaaagt ttccttatat + 11821 tggataaaga aaaagcatgg attgttcatg cagactcctt ggtatccgta gctgaggaat + 11881 tagaaggtta tttaggaatg tctgtagacg attttatcat gatttcactt ggaacacagt + 11941 atcataagcg tttttgcttt aatgacgatt acgagatttg ggagagatac tttacgaaat + 12001 taagagaagc ttattactct gactcatcct caaagtaaaa atttcttaac aagttggaat + 12061 catagagtag acagatgaaa gaagaattga tatgacaaaa gtacttaaaa aattgataga + 12121 agaaaactta tcacctctag gaattgtaga gttgattgat gaacgatatg ataagtttgg + 12181 gggatattat ttattatata gtctctcatt gactgctatc accccagaat tttcagaatc + 12241 aggaactgtc gatattgaag ttagttttag cgattatcag gagctgactt acgagttggg + 12301 caaatattct atcccctttt atgttaacag taaagaagaa ctgaagtgct tattggaggg + 12361 aatagtagat cttccactcc ctttttcagt tagttatgaa ccaattaagg aggtaacatg + 12421 ggataggtat atagttagta ttgatggggt taaaaccaca cggataattg aacttgttac + 12481 aatgataaaa gtcttagaag attacctcag tcaaattgaa ccgttactga atcaaaatgt + 12541 tttaaagata atgaagaata gggaaaataa ttagctgctt ttcatttaca gagaggatgg + 12601 tgagttcatt gaaataccta ttactggcag aaaagccaga tcaagccaaa aaatatgcga + 12661 ccgctttggg gaatcctaaa aatgagaaag gcgtttggcg tgttcagtcg tctgtattaa + 12721 atgctgaagt cattgttgct ccagctgtcg gtcatttagt tgaacgaatt aatccatata + 12781 ctaactttga aaattgggag atgactaatc ttcctgcttt gccagaaggc ttttcttatg + 12841 agcctaaaaa ggatactatt aaacggttta atgctatcaa aaaagccgtt aaagaagtag + 12901 atgccattat tattggtact gaccctgacc gtgaaggaga agccattgct tatcggattt + 12961 tagaattaat cccagaggca ttaagaaaga ttaagtatcg tttatgggca aattctttaa + 13021 cgaaaagagg attagaacaa gcttttgcta acttaagaag ccctactgac tccattaact + 13081 attttcatga ggcggacgct agaagtgatg cagactggct ggttgggttt aatttgagtc + 13141 cttttgtaac catcaaaatg aaagaagagg gttacctgga taaaaaggat cattctatgt + 13201 cagttggacg tgtacaaaca cctattgtta gtctaattgt tagaaatgat gaagcgattg + 13261 aaaactttaa accaagtcct tattggcggc taaaacttat tgattctgaa gctgaagtta + 13321 tatttagcaa tgatattaag tatcaatccc aaatggaagc tgaacaagtc ctacaacagt + 13381 tggccaatac agctgttgtc aagacggtta cgagtgaaga aaaggcacaa gaagcgccaa + 13441 aactttataa tctaaccaat ttacaatcag aaatgagtaa actatatcat tttgatgcca + 13501 ctaaaaccaa agaattggtt caaagtctgt atcaaaaagg gtatctttct tatccacgta + 13561 ccgattcaac cttgattaca accaatgagt tttcttatct ggttgatcat atcgaagagt + 13621 atcaaaaagc tattaataaa caactagaga cccctaatcg atcaccaagg aaaaactatg + 13681 tgaatgataa gaaagtgcta gaacactatg ctattattcc aactgagaat attcctgatt + 13741 tgagtgaatt aagtgatgat gaaaagctca tttatcaaaa agcggtcttt agaactttgc + 13801 tgatgtttac gcctgattat cgttaccaat caacaagtgt tattctcgat aatcatggac + 13861 ttgaatttaa agcaacaggt accgtaacga aggataaagg ttggcgtggg tatttcgcag + 13921 tcaaaaaaga agacaaagag ttacctcatt accaggaggg gcaagaggtg attgtcgatt + 13981 gtaacctttt agaagaaatg accaagccac caacacgaat caccgagcaa attctactaa + 14041 agaaattatt accaaaatac catttaggga cgtccgcaac tcgtgatggg atgattgatt + 14101 taattcagga taaaggatac gtcactaaac ataaaaaaac agggcaattt ttcccaactg + 14161 aaagaggaaa actattaatc cactatcttg accaactaga gattgcttat actaatcctg + 14221 aaacaacagg aaaatgggaa gaagtcttag ctcaaattgg tcaaggaaaa attcaaaaag + 14281 aagcctttgt caacaaaatc aaatgggcga ttaccaaaca aattgaaaaa ggcaaacaac + 14341 tttaaacgct actatttagt taacatagta gcgctttttt atggaaagga gtaacgaatg + 14401 gcaaatatgc aagaattgct ttcgcaacgg tttgaagctc aaaggcaaaa actcgttagc + 14461 atggatattg tggcagttgc ggaatcttta ggaatgacac taaaaccagg ttccagtggt + 14521 acctattatt gggaagaaca tgatagcttt catatctacc ctaatacgaa tacgttccgc + 14581 tggtggtcaa gaagtattgg cacaaatact attgacctgg ttcaagtggt acaagaagaa + 14641 atgacaggtc ataaaccaag ttttagagaa gcagcagcct ttcttgaaac tggtcacttt + 14701 gagaatgtaa cagtacaacc aattgtgaaa gaaccttttg agtattatct agagcgctat + 14761 gagcaccctg attttaatat tgggagacag tatctgaaag aagaacgtgg tttgtctgat + 14821 gaaacaattg atacgttttt agcgtcaggc aatatggcaa ctgcaacacg taaaaaaggg + 14881 gattattttg aacctgtaat tgtttttaaa agctgtgaca atgacgggaa aatgattggc + 14941 gccagtctgc aagggcttgt tgaaaatcgt gtacagcatc ctgaacgagg acggcttaag + 15001 caaattatga agaattcgga gggaactgca ggctttagtt tagatgttgg aactcctaaa + 15061 cggttagtct ttgcggaagc cccaatcgat ttaatgtcct attatgaggt caacaaagac + 15121 gacttacaag atgttaagct tgtggctatg gatggtctga aaaagggcgt tattagtcgt + 15181 tatgtcgctg atatgctaac ggatggtaaa tactctcaaa ccatgtcagc tgaacaaatt + 15241 agaggtgctt tggatgcgtt aaatcagacc actaatctgt tacaagagca ttcagatatg + 15301 attacgctag cagttgataa tgatgaggca ggtcaaaatt ttgttaaagg cttgcaggat + 15361 gatggtattc ctgtgatacc agatttacca ccacgtcaac cagctcaaaa gaaaatggat + 15421 tggaacgatt atcttaaaca aatgaagaaa gagagcgcac aaatggcaga aacaccagaa + 15481 gtagaaaaac aatcactttc tgagctccaa gaaagcttat caaacgaagg tgatttagtt + 15541 agtaccatta caagtgatag cagtattatt tatacgaata cagtaagttt taaccaagat + 15601 tataacctta atttagagat tcatagtcct aatgaagtag ataatctctc agatattcaa + 15661 gcaccttgga cacttgaagt tatgaagaaa ggtcagtcac ttggctatct tgcttatggt + 15721 gaagattggg gcaatgattt tgatattgaa gatgagttag ttaatcttga aaattgggtt + 15781 aaagacaacc aagttactaa tcatttatat actcaaaaag atattaatgc gtttttagct + 15841 agcacacaag aggttgagcc caaaatgggt gactttgtcc ttaaaggtgg tggtatttat + 15901 gatttcggaa agagtcagct tgaaggagaa gaattacgtc aacatgccat tgatgcaatg + 15961 atttctgata ttacagatgc tgaaacctat tacctttggc acgatgaaga gttagaaaaa + 16021 ctaaatgcac ccgacgaagc tttcttgaat ttccattacc atttacaaga tattcaatac + 16081 aaccaaaatg atattcatct ttatgtgtca gaatcatcaa ctgatggtgt tacaggttat + 16141 ctttcactag acggaaattc tcttgatagt gatagtattg aagagtattt agctgatcaa + 16201 gattggacgt ttaatcaaaa agtacagttt cttaagaatt taaaaactgc agttgatgat + 16261 acctggaata aagttactga ccattataat gaacaattta atgcaatcgt tgaccagtat + 16321 gggttatctt tacaaaaaga aaaaactccc gaagtacctc aagagcaaga aagtgctaat + 16381 tcagagcaac aaaaaaaggt tgagaaccca atcggggatt tacctgagca aagtcaggag + 16441 gcagcacctc tacctaatac tcacgaagag tactctttga atgagtcgtc accgactcaa + 16501 actcagtctc aacctttact tcattttaac atttctaatc cagataagtc aatttataag + 16561 gtcggttatc atccagtcaa tagaaaagag ttaaacaaat taaatcgcta tgcagcccaa + 16621 attcaagaaa atgcaacctg gtataagaac gaattggctg atagtaatgt cacttatttt + 16681 tataaaaata acgataatct agaagcttta aaggttactt ttaaagcaga gcagtaccca + 16741 catctcatag gtttctttgc aattgatgaa aatcaaagcg caagtaaaac cttggatgat + 16801 ttagcatctg ggaaaggaga ttatcaaaat attatggttg ccaaccgagg ggctaccttt + 16861 agtaaaattc aagtattacc tgattttaaa gctgttgttg attcaaatag ctttattttt + 16921 gatgatttat cagaagtaga acgcatgaat cgtcttgact tagcaaaggc tattcgaaca + 16981 gaggataagg atgttttgat tgcttttaga aatgttgatg gtgagtatct tcctgcatct + 17041 cttatgaaag ttactaataa gcttgagtca gagttgtcaa tcgcaaataa taaagacgtt + 17101 ttaggtattg ttggtgagaa aaatggagag tttaaagttc tttcagtcaa tgaagaaatt + 17161 atcaaagatg gtggtaaaaa aatgttatca attgtaaaaa ataaccaatt tgaaaatctt + 17221 tctaaagcag aagtgactgc tgaagacttt actaaagttt tagatgctgt ttacaatgtt + 17281 ggtgctcaag ttggtaaaga taacagagct aatattccca aagaattgta tccagcttgg + 17341 gataaatact atgaatatgc tgaaagatat gataacaatt ttgatgttat tgttaaagca + 17401 gcaagagcag ataatctctt tgatgaaaat tctgattttt ataaagatgt ctggcttcga + 17461 aaaattcaag aagaaaatca gaaagtaaaa gattctgatg gcgatggtct aaccgatgat + 17521 gaagaaatcg cattaggaac aaatccttat agtccagata ctgatggtga cagtgtcata + 17581 gatagtattg aaagaggaag tggaacagat gcgacgaatc cttctgatac accagacaat + 17641 cgacaaaaag aacaaatgaa acacaactta accttgtcgg aaatgattaa agctaaagat + 17701 accgttgctc ttaatcagca cttgcaagaa gggattaaag attactttga tagtgatacc + 17761 tataaaaatt atttggaagg tatggcacac ttcaatcact attctgctcg taacattcaa + 17821 ttgataaaag cacaattgcc agaggcaaca atggtcgctt cttttgaaga atggaaaaag + 17881 agaaatggtc atgttaacaa gggtgaaaaa gctctatatg ttcaagcacc agtaactgtg + 17941 attaaaaaag atgctgacgg taaccctgtc atcaattcag aaactggtga aaaagaaact + 18001 ttcacttact ttaaacccgt tcctgttttt gatattagcc aaatttctcc tcagcaaggc + 18061 aaacagctta acctgcccaa atcatctgaa gcaattcctg cccaacttaa taaagattat + 18121 tatcaaaatg tttatcgagc gctaagagat atctcccaaa aagaaaatgg tattcccatt + 18181 cgttttagag aactagagca attcgatggt ttctacaatc ctaaaacaaa tgatattacg + 18241 atcaaaaaag ggatgaccta tgaacaaacc ctttcaacac taattcatga aatggcacat + 18301 tctgaattac acaacaagaa gagtttgaca gaacgttttg aaggtaaact aacacgttct + 18361 tcaaaagaac tacaagctga atcagtcgct tatgttgtgt caaaccattt agggtttgat + 18421 actagtaacg attcgtttgc ttatcttgct tcttggtcgc aagaaccaga cggattagaa + 18481 aatctaaaag ctcaacttga aattgttcaa gaagaagcta gttcactgat gaaccgcatt + 18541 gataagcagt taactaaata ccaaacgttg acggtttcaa aagatgaaac gttaaccgaa + 18601 actcaaaaac gtaacttaga gaagtcagca aatccctttt atcaaagttt gcaaaaagcc + 18661 aaagccgaag ttaacaacat gaaaaaaggt gatgaaccta acaatgagca aaccgtaaaa + 18721 aaagacaatc gacctacgaa gtgttaacac agcgtaggtc acgtcgaaaa caactaaccc + 18781 cacaaggagg acaatatgat aacagacgaa atgatgacga taactgaaat gatattaatg + 18841 agttattact ttgatatgag cgaatggctc aaaggaaata aaatcattaa tagtgatatc + 18901 gttcaaaaat caaaagatga tttattggat gtgcttaaaa ctgattttga acaactcaca + 18961 accgaagata ataacgatta tcttgatgac ctctcaatca gtattggcac tcttgaagag + 19021 ttgtcagaag ataactacca aaaattaaaa acagctattt tttcttggga gccaagcaag + 19081 aaaaaataaa aatcgtaaaa gaaggtggct gacaattgtt caattgtcag tttttctttt + 19141 gttgcatttt accaaaatgc aagtatcaaa aatggtcatt tttgaaaaat gcacatcgtg + 19201 cgagaaaagg ggtttgggga tttccccaaa atgtaaaaag tcaaaagtcg gtttgtaggg + 19261 tttctgcaaa ctggcttatt gaccttttta ttttcgcaag tggtgcccca cttgctacgc + 19321 ttgccagagt aaaagtaggt ctggcctaag gcttagctga agagctgaca ttataacaaa + 19381 ttgtatagaa tggagagaat aatgacgata aaaagacgca aacgtccaat tgaatttaag + 19441 cttagaacaa ctgatgaaga agcgtttcaa ttacgccaac ggataacaat ggctaataag + 19501 aaaacctttc aaggctatgc gttagagatg ttgttgaaag gcaaaattga aacctatgat + 19561 tattcagaat tacaacggtt gcgagtagaa gttaatcata ttggtcaaaa cgttaaccag + 19621 ttagtgcgtt atgtgaatac gtttgatgag attgatagtg aattgtttaa agctttacaa + 19681 gctgaaattg atgagatgaa agtattgctc attgatgaat ttaaaacgaa aggtcgggca + 19741 aaattaaatg gtggtgacaa aagtaatcca gattaaaagc agtcgtaact taaaacgggc + 19801 aattaattat attacacgtg ataatgcaac attgaaatta gatagcgaac gtttagaagg + 19861 tgatgacaat tattcttatg agatggtcaa tggtcaagtg atgaaacggt tggtatctgg + 19921 tcatgatgtc accgatattt cagatccaca aacaatttat gatgattttg ttttgttaaa + 19981 acaatctgtt gatgctcttt ataataacga tgagctatcc gatttgaaaa atgataaacg + 20041 tgttttagca caccatatca ttcaatcgtt ttcgccagaa gatggcttaa ccccagaaga + 20101 ggttaacgag attggacgaa aaaccgcttt agagttaacg ggaggtgatt atcaatttgt + 20161 cgttgcaaca catatggata aagggcattt gcataatcat attattttta acacaacgaa + 20221 tgaagtgact ctgaaaaagt ttcgttggca aaaaggaacc aaaaagagtt tagaacatat + 20281 ttcagataag tatgctgagc tttatggtgc aaaagtttta gaaccgagac ttagaaattc + 20341 tcacacagat tattcggcat ggcgccgaaa aaataatttt cggtttgaga ttaaagaacg + 20401 attgaatttt ttgttaaagc actctttaga tatgactgac tttttacaaa aagccaaaac + 20461 tttaaattta caagttgata cgagtggtaa atatattaaa tacaaattac ttgaccagcc + 20521 acaagagcgt tttgttcgtg accgtacgtt atcaaaaaaa ggaaagttct cacttgaaaa + 20581 aatcaaagaa caaattgcaa caaacgaagt tgtttatgat ttaaacgtca taaaagaaaa + 20641 atatgatgaa gagcaagaaa gtaaacaaga tgattttgaa atgcagctca ccattgaacc + 20701 atggcaaatt gaacaactca ctcatcagtc aatttatgtt cccattactt ttggtttaga + 20761 tcgaaaagga acggtttcca ttcctgcacg gatgttagac caaaatgaag atggaacgtt + 20821 tacggcttat ctcaagaaaa atgatttctt ttatttttta aatacagatc attcggaaca + 20881 aaatcgtttt attaaaggag tcactttaat caaacagtta tcggctcaaa atggtgaaat + 20941 gattttaaca aaaaataaaa atatcactaa cctagatagg ttagttgatg aatttaactt + 21001 tttagcagca aacaacgtta ccaattcaaa acaatttgaa aatctgcaac aacaattttt + 21061 agagcagctg gatgagacgg ataaaacttt agccgcactg ggtgataaga ttttttatct + 21121 taataaattg ctaggagcat tatctgatta tcaaaataat attgttccat cagaagtgag + 21181 tcttgacctt ttagaaaaag gaaaaattga taagacaatg aaacttgacg agttacaaaa + 21241 agaaattaaa gagcttcaaa ttgaacgtga cacactcaaa aagcatagag ataagattgt + 21301 aaaagattat gactttacta aagagatcaa acaaacgcat gaaaaacgca ctggaatatc + 21361 attgtaagca atttaacaaa agggatagtc tcaaaaagat agtaactaac tttttctata + 21421 gtgcttttta tctgatataa taaactaaat gatacaaaag gaaatgatac tatggcaaca + 21481 aataaagaac agattaaaaa ttcaggaaag tgtctctttg ttgagacgac cacaaaactt + 21541 tcaagtgatg agttattaaa agcaatagct gatgacaaca tgacagaaga gcaagtggaa + 21601 caattaaaaa ttgatctgga tatcttttta aaacaaagag cagctgcttt caaggaatga + 21661 ggtgaggaat tgcaaaaaca taacctaata aaactattaa aggggcttct cttggtgcta + 21721 ttattgctta taggagtagc cttaattttt aacaaatcta tccgaaatac cttaattgcg + 21781 tggaatacta atcagtatca agtctcacag gtttctaaac aaaccattga gcaaaataaa + 21841 gacgctaatg tgagttatga ttttgattcg gtcgaatcca tttcaaccga atctgttcta + 21901 aaagcccaaa ccaatagcgc taatcttcct gttattggtg gggttgctat tccagaggtt + 21961 gggattaatc ttcccatttt taagggactc ggtaatacag aattgactta tggggcagga + 22021 accatgaaag aaaatcaagt catgggaagt ggtaactacg ctcttgccag tcaccatgtg + 22081 tttggcttaa caggttcttc tcaaatgctc ttttcccctt tagaaaatgt taaaacgggg + 22141 atgaagattt acttaacaga taaagaaacg gtgtatactt acattgtcac atcaattgag + 22201 agtgtcgcac ctagtcaagt agcggtgatt gatgatacgg aagagacaga agtgacccta + 22261 gttacgtgta cggatgctga agcaacctca cggacgattg tcaaaggagt ttatgactct + 22321 gaagttagtt ttgaccaagc accggaggac attttagacg cttttaatac agcttataat + 22381 caaattgctt tataaagaca aataaaaaat aggcagtagc tattctaatt agggtagctg + 22441 ctgcctattt ttgtgctttt caagtgttaa aggtacgctt gtgttaattt taagggattg + 22501 aaaagcattt tggctattat aacatagttt cttaaaaggg aaaaggtgat tacaaacttg + 22561 taaccacctt aggagatttt tgaaaagttt ttttgggata ataatagtat agcaattttt + 22621 ctttttataa gctaataatc agcatgttat gtcacaaatt ttcaaaaatt cgttaaaata + 22681 gaactatggt agtaatgaga tttgatgaaa gaggatttgt agaagaactt tatagacaac + 22741 gggagtatga gttcttaaag cgagtcgcag cctatgataa acaaatagct cccactatgc + 22801 gagcaaacgg ttataaaaga gtagatagca gtgagcgaac tgtgttattt acgtttggcg + 22861 caatgacatt ttcaagaagc cgctggcgac gagggacgaa aacccgttat cctgtggatg + 22921 aatggttggg gttgaagaag tatatgcgct gttcgttgga acttatgtat catctggtaa + 22981 gatatgcttc agagatgtct tatcgccaag tttgtcggat gataaagcta tcttaccact + 23041 tagatattac aaaagatacg gtcttaaaag cggtaaaatt aacaggacag ttattctctg + 23101 aaaagagcca ttatcgttac tttgttgagg aagaggtatc agaaaaaatc aaagcaccgg + 23161 ttatttatat tgagggtgat ggtgttctgg taaaaacaaa tacgggtgga gatgagaagc + 23221 acaatacgga tttagcacat tttcttgttc atacgggaac aaaaatagta catggacgaa + 23281 cagtcttgct taataaacat gaaattattc atacagacta tgaagttgcc agagatgaat + 23341 tactagatta tctctataat cattttgaaa tcaccaatca aactattcta gtgactaact + 23401 cagataatgg caagggctat accagacgtg tctttcaaaa tattcaaaag gcgctcaaaa + 23461 ttaaacgtca tgaacacttt tgggacgccc atcatgtcaa ggaaaaaatt acttcatttt + 23521 ttaaaagtta tcctaaaata ctgaaagatt tgatgtttaa agccattcaa acccataatc + 23581 gaaaattatt aaaaactgtt ttggatacag ttgaatcatt ggtcactgac gatgaagagt + 23641 acctagtttt tcaaaagttt aggaagaaat ttttgaataa ttttaaggat acaaaacctg + 23701 ctaaactaag aggtctctcc cataagggaa ttggggtcat ggaaagtcag cactgtaaag + 23761 taacctttcg catgaaacat cgtggcatgt actggtcaat taaaggtgcc tgtgctatgg + 23821 ctagacttat tctgttagaa cggattgatc aattagaaaa cctctttttt ggcaattggc + 23881 gcaaagatta tcagtactat aaagataacc agttaggtgt tggttattta agggagtcga + 23941 aagccgatca ttctcccacg cctcaaagaa tctttaaacg agctggtaaa atcatgtcat + 24001 tagatcgtca aaagtttaaa tattaac +// +LOCUS NC_020450 18869 bp DNA linear CON 24-DEC-2022 +DEFINITION Lactococcus lactis subsp. lactis IO-1, complete sequence. +ACCESSION NC_020450 REGION: 643089..661957 +VERSION NC_020450.1 +DBLINK BioProject: PRJNA224116 + BioSample: SAMD00060995 + Assembly: GCF_000344575.1 +KEYWORDS RefSeq. +SOURCE Lactococcus lactis subsp. lactis IO-1 + ORGANISM Lactococcus lactis subsp. lactis IO-1 + Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae; + Lactococcus. +REFERENCE 1 + AUTHORS Kato,H., Shiwa,Y., Oshima,K., Machii,M., Araya-Kojima,T., Zendo,T., + Shimizu-Kadota,M., Hattori,M., Sonomoto,K. and Yoshikawa,H. + TITLE Complete genome sequence of Lactococcus lactis IO-1, a lactic acid + bacterium that utilizes xylose and produces high levels of L-lactic + acid + JOURNAL J Bacteriol 194 (8), 2102-2103 (2012) + PUBMED 22461545 +REFERENCE 2 (bases 1 to 18869) + AUTHORS Shimizu-Kadota,M., Kato,H., Shiwa,Y., Sonomoto,K. and Yoshikawa,H. + TITLE Direct Submission + JOURNAL Submitted (28-SEP-2011) Contact:Hirofumi Yoshikawa Tokyo University + of Agriculture, Department of Bioscience; 1-1-1 Sakuragaoka, + Setagaya-ku, Tokyo 156-8502, Japan URL + :http://nodai.cc-town.net/laboratory/single.php?id=23 +COMMENT REFSEQ INFORMATION: The reference sequence is identical to + AP012281.1. + The annotation was added by the NCBI Prokaryotic Genome Annotation + Pipeline (PGAP). Information about PGAP can be found here: + https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ + + ##Genome-Assembly-Data-START## + Assembly Method :: phrap v.1.080730 + Genome Coverage :: 8.75x + Sequencing Technology :: ABI 3730xl + ##Genome-Assembly-Data-END## + + ##Genome-Annotation-Data-START## + Annotation Provider :: NCBI RefSeq + Annotation Date :: 12/24/2022 01:48:57 + Annotation Pipeline :: NCBI Prokaryotic Genome + Annotation Pipeline (PGAP) + Annotation Method :: Best-placed reference protein + set; GeneMarkS-2+ + Annotation Software revision :: 6.4 + Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA + Genes (total) :: 2,359 + CDSs (total) :: 2,271 + Genes (coding) :: 2,261 + CDSs (with protein) :: 2,261 + Genes (RNA) :: 88 + rRNAs :: 7, 6, 6 (5S, 16S, 23S) + complete rRNAs :: 7, 6, 6 (5S, 16S, 23S) + tRNAs :: 65 + ncRNAs :: 4 + Pseudo Genes (total) :: 10 + CDSs (without protein) :: 10 + Pseudo Genes (ambiguous residues) :: 0 of 10 + Pseudo Genes (frameshifted) :: 4 of 10 + Pseudo Genes (incomplete) :: 5 of 10 + Pseudo Genes (internal stop) :: 3 of 10 + Pseudo Genes (multiple problems) :: 2 of 10 + ##Genome-Annotation-Data-END## +FEATURES Location/Qualifiers + source 1..18869 + /organism="Lactococcus lactis subsp. lactis IO-1" + /mol_type="genomic DNA" + /strain="IO-1" + /sub_species="lactis" + /db_xref="taxon:1046624" + gene 2..1684 + /locus_tag="LILO_RS03250" + /old_locus_tag="lilo_0603" + CDS 2..1684 + /locus_tag="LILO_RS03250" + /old_locus_tag="lilo_0603" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_015426013.1" + /GO_function="GO:0005524 - ATP binding [Evidence IEA]; + GO:0003677 - DNA binding [Evidence IEA]; GO:0000166 - + nucleotide binding [Evidence IEA]" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="FtsK/SpoIIIE domain-containing protein" + /protein_id="WP_015426013.1" + /translation="MAKRTISKGSRLKLRDKYDREFFVLITGLLFASPVGFFYKNFET + LPPKVLAIYAVDILLIAFSVAFGLLILLYHKFLFFSKNNYKRLLLNYVVKHGLVEKEM + VKDEKGSHEKLKLAPIYLKQPNTYELHTYFPIDGGVHQEKFLDLADGLETTFFADYQE + QNFINESKILSKKAFVEYVFAIDGERNRISVNDVIVDKKLGLKLMNGVYWNYEADPHM + LIAGGTGGGKTVLLMSILSALAKVGHVDICDPKRSDFVGMRDVPVFENRVFFDKESMI + ECLRSKMQFMDERYDYMTNHPDYKAGKRYSDYCLTPEFVLFDEWAAFISSLDFREFDE + VIQILTQIVLKGRQSGVFLILAMQRPDAEYLKSALRDNFMKRLAVGRLTGSGYRMVFG + DENEKKVFKYIKGKIGRGYVANNGELAREFYSPSVPFDKGYDFHEELSKLPVLADTTE + VQLEAPPISNVEQERAESELLEEKFYTITSLSRKLGQPSKTVKTAIERLTSGGYSISE + KSPYNEDDFIALQTVFLTKETEECTLNEAVDTVLADEAEFEKEFVTFDKEVA" + gene 1706..1876 + /locus_tag="LILO_RS12075" + CDS 1706..1876 + /locus_tag="LILO_RS12075" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_003129468.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_012897120.1" + /translation="MAEKSVRVTISKDNKTTELTLRGVAVKDFEKLFFAVQNQMKSCE + KKEDKHHAIGGE" + gene 2314..3468 + /locus_tag="LILO_RS03260" + /old_locus_tag="lilo_0605" + CDS 2314..3468 + /locus_tag="LILO_RS03260" + /old_locus_tag="lilo_0605" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_014570871.1" + /GO_function="GO:0003677 - DNA binding [Evidence IEA]; + GO:0003916 - DNA topoisomerase activity [Evidence IEA]" + /GO_process="GO:0006270 - DNA replication initiation + [Evidence IEA]" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="replication initiation factor domain-containing + protein" + /protein_id="WP_015426015.1" + /translation="MADLLGISLVYYRKMENGDRPLSKQFEEKIRNSFFKKRESSTVF + VGTNDYTNIRFQTLNVREVVSKILGLNVENFQLNEYNRYQYPFYISYGHINVYYHDKD + IKAGVLIEMSGQACREMEYEFEYHQKQRTWYDFFNDCFLYANKKAPENDDFVKITRFD + LALDEQYNPQEGNFDLFKLLTSAREGRWNGRKQNYSAVLGGRRTKEGMINDGLTVYFG + SKQTHLFFRFYEKDYERASQEMTSVEAIREMYGLRNRYEISMRKEISTDFIKRYIEED + FDLADEGVKIINDNLTFYDKEGNLDSEWYDMMGRMDAYHFTVRPEAPDLNRKYTWFER + GGPVSTYLLLKKAEELTGESRLEEIFNEAELTERQEKFLKEFRMIRGANG" + gene 3604..3933 + /locus_tag="LILO_RS03265" + /old_locus_tag="lilo_0606" + CDS 3604..3933 + /locus_tag="LILO_RS03265" + /old_locus_tag="lilo_0606" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_014570869.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_015426016.1" + /translation="MIKPNIEHFQKRMLRVPNNVRMLQYKMSGYEFNLKKFEQGVYDQ + LFFYIPNETEALGLLNEIGLMFPPDSMTRKKYYSLYQEKRIADLPEGYKMAIYFLIDN + DLAQATL" + gene 4020..4331 + /locus_tag="LILO_RS03270" + /old_locus_tag="lilo_0607" + CDS 4020..4331 + /locus_tag="LILO_RS03270" + /old_locus_tag="lilo_0607" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_019293439.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_015426017.1" + /translation="MPTLKTGLKQLTTDLNEIEAVINAGNAYQEAVSKLKGQGKALRT + LENDTSISDDTVEVIANRTNDLIRNLLKGSTAYNFTKKSFNELKGKLEEAEAQARSGA + K" + gene 4463..4723 + /locus_tag="LILO_RS03275" + /old_locus_tag="lilo_0608" + CDS 4463..4723 + /locus_tag="LILO_RS03275" + /old_locus_tag="lilo_0608" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_003129681.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_015426018.1" + /translation="MTVTNMSKARPQIDFSLHKAPYKDLYDIQILFPDGTNQFWSGIN + EKKVMWLCEKYNPISTPLSIAIAPRFLPKSNIQKPNWEIRKG" + gene 4829..5038 + /locus_tag="LILO_RS03280" + CDS 4829..5038 + /locus_tag="LILO_RS03280" + /inference="COORDINATES: protein motif:HMM:NF024252.2" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="helix-turn-helix transcriptional regulator" + /protein_id="WP_042230155.1" + /translation="MSVFAERLKELRKEKGLTQSQVGVHLGMSQQNYRRWEVGERSPS + GETLIKLADYFDVSTDYLLGRKSEK" + gene 5035..5331 + /locus_tag="LILO_RS03285" + /old_locus_tag="lilo_0609" + CDS 5035..5331 + /locus_tag="LILO_RS03285" + /old_locus_tag="lilo_0609" + /inference="COORDINATES: ab initio + prediction:GeneMarkS-2+" + /note="Derived by automated computational analysis using + gene prediction method: GeneMarkS-2+." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_015426019.1" + /translation="MNIQQLYLIINKMNKVLKVARLISLIPVTLIGLAFEILTSMFLS + GTICWWLAAFKIISENNKIIFYLFSVIIFLILLVYSLIGILKVLENKLKEKEVK" + gene 5331..5750 + /locus_tag="LILO_RS03290" + /old_locus_tag="lilo_0610" + CDS 5331..5750 + /locus_tag="LILO_RS03290" + /old_locus_tag="lilo_0610" + /inference="COORDINATES: protein motif:HMM:NF024363.2" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="DUF3850 domain-containing protein" + /protein_id="WP_168694145.1" + /translation="MTVHKKKLDFQFHKPVETGEKPFEVRINDCDYQVGDIIELKSYG + KCCNYCDGKSYKKYYSGNCSGWMNCYESEAETIRVKILRVIYPKEINYALTHGVILSS + SFGKINLDDVREVLKEYFNRKTLPKDYVLLEIEVIKK" + gene 5846..8254 + /locus_tag="LILO_RS03295" + /old_locus_tag="lilo_0611" + CDS 5846..8254 + /locus_tag="LILO_RS03295" + /old_locus_tag="lilo_0611" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_017865118.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="SpaA isopeptide-forming pilin-related protein" + /protein_id="WP_015426021.1" + /translation="MSKHKKLLNGLTSEKKVTNWKMHKRKKVFVAFAGVLAVGLGTAG + LVYASAQSTSAKASLPTDTTVNWENNKPLYYEIDQTGKEHPKPMLKLSDGTPAWCLGL + GVPLVNNITQAQLDSSNAILNALSDEQIAVINNVAYLAQKQGTLLAYAQAQHATYLLL + DEAGVSVNQTKDLIVKPNTLLQEPNAIKTGANDLITQAKALRKLPSFHGTTVDVVQGV + EKTVTDSNSVLANFPNFKSNVSGLSESVSGNDLKLKADITAKVGITPKAIQFQNTPLS + IADLPYYVFSTDGDSTGKASQSVIASQDPSKANGYLNVNVIGLGETTLTKVDADTGSG + ETQGAAQLKGAVFGLFNKSDNSPVKWSDGQKGYPITVTAGTKADDTNVSIKLGDDLKA + GLKNLDNSKEYYWKETVAPEGYSLSTEKYEVKFDSSSKFDDKTSNFIDDDKATDKVLD + LNFGFIKAQDVNGSLTGLNGRTFRYTPTGDTKGKPIEVTSGANEDSNGVTNNGQVNFT + KIPFGDGLLEELPQKDDKLQLINPISIVTTTNKDKDENITGYTVTFTDTVTKQVITTL + DVSLDKVTDNSTMFKVNLGTLVDKPVTPVVPTIKTKAHTKDGDQTIQKVEISKTTPLY + DKIMTTNAIKGDQMVATLHRVVTGKDGKVTESKVIRTLNFTVDDETVKAQEKQIESTV + DTTKDADVPETSTVAYVWTEDLFDEGKNPKTDEPKAKHDDLKDQDQTLTVEKVEKPVT + PPSSKPTPPSTTPTETPKVSLPTTGSETGDMIMYGGALAVLIALGTGAVYYMKKKKST + KEEG" + gene 8381..8569 + /locus_tag="LILO_RS03300" + CDS 8381..8569 + /locus_tag="LILO_RS03300" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_014570866.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_042230157.1" + /translation="MYVVKMRGGYLCANGGATKHLKFATTFDTKKKAEEVAEKWLRSD + VSFKAVEKESEEYEQNKN" + gene 8550..9821 + /locus_tag="LILO_RS03305" + /old_locus_tag="lilo_0612" + CDS 8550..9821 + /locus_tag="LILO_RS03305" + /old_locus_tag="lilo_0612" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_014570446.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="translation initiation factor IF-2 N-terminal + domain-containing protein" + /protein_id="WP_042230159.1" + /translation="MSRIKISELADEFKIDRKDVLAKAQEFGVSAKLATKTLSESEEK + QLRTYYQQVPHSMAETSKNQVLENVGQKKLDPKEEVSEKKEEPSKAEQVEKKKSKFSI + LGHKKSEKKKGKLLTRLSNEKPPSKKQFTAKQGGALVVGGVGILILSNMVLFGLMASG + YQPTQKIIHEQVSASQKVSGNGLDLQAKNYLDGFVQTYFTFPENEKDQEQAVKDINGY + FVQQLPVISQGLQRSPSKFEEAVLMNLTDNEATYKVTYGAGAVTKKGKSNVVNYHDVT + TLFTIPYQKVGSSYYVSDEPYFSSVNDLQAKESQVPVKTWSGTDNNSASTKKDLDKFT + KALFTAYTTDSDTLKLISEGLSLNDSETFQSLDQATYEPKGDTYQAIVQVTMKNELGT + HVENYQFTISKQKQSYFATDFKHTLPKGNKE" + gene 9834..10097 + /locus_tag="LILO_RS03310" + /old_locus_tag="lilo_0613" + CDS 9834..10097 + /locus_tag="LILO_RS03310" + /old_locus_tag="lilo_0613" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_003131793.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="TcpD family membrane protein" + /protein_id="WP_015426023.1" + /translation="MKELLNYHIAVVYGDIGLSNFWNTFQTNVKYIIFAGTLWFMIRE + WKNKAVGKMVMTFIVGAILFALTLNPETVLQPIGQKLMQMIGA" + gene 10111..10506 + /locus_tag="LILO_RS03315" + CDS 10111..10506 + /locus_tag="LILO_RS03315" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_003131794.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="TcpE family conjugal transfer membrane protein" + /protein_id="WP_042230162.1" + /translation="MSKENKKGLKYSNKSSLSQPLRIQKLFDGWSLGKAWRLSFFIYF + GLFGFILWKLLFSKLVVFPIGFRLVVESFMCYKSALFLAEIRIDDKSPFIYLKDMISF + IITFGLKGKSIYKGWIYPLRKRKEELKEK" + gene 10503..13112 + /locus_tag="LILO_RS03320" + /old_locus_tag="lilo_0614" + CDS 10503..13112 + /locus_tag="LILO_RS03320" + /old_locus_tag="lilo_0614" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_014570447.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="ATP-binding protein" + /protein_id="WP_042230164.1" + /translation="MKKKATWENHILSVHENLVLKDDGTVFAMFEVPASIISMMDEKG + KKNHKQTTQAVITSLYEHLGFEILMKPITKDLLKAYGYLLEDCDPRTREFGEYLLEQS + YLELENELGKIYNHKFFLTVPLSDFAFSGDLKKMMKEGWQRFRKSVVQLIRRELEFTV + NWYEDYEQTALDLEMNLNLLQARPLKQEETLLYNSLNYLRGIDVNQDELLADVNNAIE + NIDDTTVDVRSDGLLEIHHPQGESLLKFLPLADYPDVVNDIHLIEHIQTLPFPVEFRV + KARFRKNKGALGMEATAERTRDRIGTELSEADEQGNVTKNSSVSAYVILEDIIAGVDG + GEYFLDFLPVFAITGSTPDEINYYKKMLMTAMEGLGIKIVPSQWDQPYLFYKMRSTEE + LTRSDKYWVQNMKVSAFVENLFFVSQKVGSDVGFYFGRVDHTQLNWAGNFEKAIASSP + TPFFWNMFQANKDDIEGKLGDSPHVGIFGDTGSGKSFFAKQGFVNHSILKGLSLYIDP + KDEMKSQFLAVRDKLESYLPEMEEKISELVVLIEDDEILARRIRNLEFSYHKPLIDYI + NHIHFVSLDVKDKKNIGALDPIVFLEGNQATELATQVTKGLIGDKLTEDDRFENCFNQ + KVQEVIEARAKGKTVGLLNVFQLMAKSEEDFIRYRAENILSKISGTMLELVFSEGQNS + SVSMDEHITILGVTGLNLAKSGEVKTAQNEMSDIIMYALGDFCRFFGARDREQETVIW + LDEGWFFNTTDIGKGILMRMKRVGRSENNFLVLISQSVKDGASEEDDTAFGTIFAFKE + TGNTTAALKAIGLPDDVEEIIKWYENMTKGLALLKDPFGRIGRVVIQGQNSALNECFK + TSFSKMKSTKAVA" + gene 13112..15229 + /locus_tag="LILO_RS03325" + /old_locus_tag="lilo_0615" + CDS 13112..15229 + /locus_tag="LILO_RS03325" + /old_locus_tag="lilo_0615" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_003131796.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="metal-dependent phosphohydrolase" + /protein_id="WP_015426025.1" + /translation="MKKKMLLVVGLLAFLGGVALPSQADTAFNVPTTPSFSSAKNPTV + SKAPTSSSSTASSKSSTTSPSYPVNNGFVGGDLGDYSSSNSPSKVMNEVEKKKARENL + DNYTSYMYFDDGKFLTGSMSAGMQTFFVRSQFFVTKTIYRFVNAVNEKLNSDSLITQW + TAQMFATVKKIYNQFSNPQLYPIIAIGVISSLLYYWFKRRFLEGVRKVILVMLLVGLF + INGGQKLTEQINSSLNTATTTLMSTVKVAGVSSRNSNDLKTTMVEVPFLYMNFDNVKI + NADGSSNISEDNIVKLLTSDDDNDKLKTIQSDLKDSHLTSKKMGEKVLTALASIFNAI + LVGFIYLAFAVMAFVMRMFFLILLLLLPFVGILSLFPFFDVVILRWAKATGGALIVSN + VVVIGTALISILDSIVSSAVTSMIGSDYFFITLIKFIVYIILFKKREKILEIFKAGHL + GNSGFAGRMDGLLSNVRRKGSNMIKAPLLAGSSAGLVAGLTAGQMATGKAKEIAKNRL + TNGTGSLWHGGLNKKADRTMNKMDKANPNSKKGQKLQAKEEKIKQRLEKRKQKFENPN + LLKRKVADYRKQYQQRKTMNNPNLAQKMEQKNKEKNASIQALYDKNKTQLQARAIERR + MREAVPDQEKPNLSELAKNKQKALAKEKLDERAMKQSAGRMAERFEKEKVSHSSFKRK + PNYPWDNNDTNQKLNNPFVIDEK" + gene 15239..16279 + /locus_tag="LILO_RS11840" + /old_locus_tag="lilo_0616" + CDS 15239..16279 + /locus_tag="LILO_RS11840" + /old_locus_tag="lilo_0616" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_017371146.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="phage tail tip lysozyme" + /protein_id="WP_080619315.1" + /translation="MKKAIKRKVILVSLPFLLLLLPILAFFSLFVGSNSSSDTDINTN + TPQQQTAKVIWDRVLKEGGTKEGAAALLGNNQAESELQPSIIQSNATYNEAKAMDTTL + GGYAFGLAQWDSGRRVNLLNYAKSQKKSWTDTNLQVEFMFEQDGTDSTLLKQLIKGTN + VKQTTEDIMRKWERAGAVDSLSKRQGFAEYWYTFMTTGGDSGTGGGSGITPDIPSGWT + LDKPINTSGYITSSYEYKQCTWFTWNRAKEFGITFSPFMGNGADWQHQAGYTVTTTPT + LHSAVSFSGGQTVGGQWNADPVYGHVAFVEAIHSDGSVLISQSGTGFSTVYTFQVLTK + AQASQLHYVIGK" + gene 16374..16868 + /locus_tag="LILO_RS03335" + /old_locus_tag="lilo_0617" + CDS 16374..16868 + /locus_tag="LILO_RS03335" + /old_locus_tag="lilo_0617" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_017371147.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_015426027.1" + /translation="MTKLEEVRASGKMSERVLENNFRHFDHRLREIEGELRLYPYATL + SEVIAWAEQLKIAIGKIKAIQESSIIKSKKEWGILEEKMLGYLQIDKAFIHVFSDHVI + FLVQLEQRYRQRLSIFANNLDNSVRYLKRYADDLEKQGFSITGILAESRNLSDMNWLS + ILNY" + gene 16894..17385 + /locus_tag="LILO_RS03340" + /old_locus_tag="lilo_0618" + CDS 16894..17385 + /locus_tag="LILO_RS03340" + /old_locus_tag="lilo_0618" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_017371148.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="antirestriction protein ArdA" + /protein_id="WP_015426028.1" + /translation="METPKIYVVNLNSYNNGRTRGKWYELPVDFSRIKSDLLLDVEHG + EEYAIHDFENFYGYKVGEYSSIQELNEYAEKLEEISDIEHLKDFLEIYSIDDVINNKD + DLDFVEAENDEDLAQELIEQMGGLEVLSVETLQRYFNFGAYGRDLAIGDYSKTSHGYI + RDI" + gene 17440..17646 + /locus_tag="LILO_RS03345" + /old_locus_tag="lilo_0619" + CDS 17440..17646 + /locus_tag="LILO_RS03345" + /old_locus_tag="lilo_0619" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_015426029.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="hypothetical protein" + /protein_id="WP_015426029.1" + /translation="MEENYKDKRGISKLFDVPIKTLNNDLTEMRRTEFNVYILRPSHK + RVYINVQGYKSFLEYKQKIRESTI" + gene 17729..18868 + /locus_tag="LILO_RS03350" + /old_locus_tag="lilo_0620" + CDS 17729..18868 + /locus_tag="LILO_RS03350" + /old_locus_tag="lilo_0620" + /inference="COORDINATES: similar to AA + sequence:RefSeq:WP_014570452.1" + /note="Derived by automated computational analysis using + gene prediction method: Protein Homology." + /codon_start=1 + /transl_table=11 + /product="site-specific integrase" + /protein_id="WP_042230166.1" + /translation="MFYKQLDSGKYRYFEKYFDEKRNKWRQVTVTLKSKSRVAQSEAK + NRLARKIEQSKKVPTAIELQEQIVQNKTIQEIYEEWVVIRKQDVKPASFVAEQISLKG + FIEKFSKYKVSEVTTADIQSYLMELDIANSTRKNRRIYIRILFKYAENIGYINSNPAD + KVVLPKVRLEIETLERANEKFLSKEEMSSVLIFCKSYKKNIRYTLAMEFIFLTGCRFG + EFASIRYQDVDFKNKLLRIDHTLEYRVAKYDDRVIQTPKTVGSIRTISLSNRCLEIID + YFQKNCLDDKFVFVNAVGGIFRQPVFYKFICDNCQKVLGNERKYGIHLLRHSHVSLLV + ELGVPIKAIMERVGHRDESITLRIYSHISGTIKNEISQKLNQINL" +ORIGIN + 1 aatggcaaaa cgtacaataa gtaaaggctc tcgtttaaag cttagagata agtatgaccg + 61 tgaatttttt gtattgatta ctggtttact tttcgctagt cctgtagggt tcttttataa + 121 aaattttgaa accttgccac caaaagtgct agcaatttat gctgtagata ttctgctcat + 181 tgcattttcg gtggcttttg gtttattgat tttgctttat cacaagttcc ttttcttttc + 241 aaaaaacaat tataaaagat tgttactgaa ctatgttgtg aagcatggtt tagttgaaaa + 301 agaaatggtt aaagatgaaa aaggaagtca tgaaaagtta aaactagcgc ctatttatct + 361 gaagcaacct aacacgtatg aattacatac atattttcca attgacggtg gagttcatca + 421 agaaaaattt cttgatttag ctgacggttt agaaacaact ttttttgccg attatcaaga + 481 acaaaatttt attaatgaaa gtaaaattct ttctaaaaaa gcatttgttg agtatgtttt + 541 tgctattgac ggagaacgta atcgtatttc agttaatgat gtcatagtcg ataaaaaatt + 601 aggtttaaaa ttaatgaatg gggtttactg gaattatgaa gctgacccac atatgcttat + 661 tgcaggtggt actgggggtg gcaaaaccgt tcttttgatg tcaattttat cagcccttgc + 721 taaagtagga catgttgata tatgtgaccc taaacgttct gactttgtgg gaatgagaga + 781 tgttccagtt tttgaaaatc gtgtgttctt tgataaagag tctatgattg agtgtttacg + 841 ttcaaaaatg caatttatgg acgaacgcta tgattatatg acaaatcacc ctgattataa + 901 ggcagggaaa cgatattctg attattgttt gacacctgaa tttgtacttt ttgatgaatg + 961 ggctgctttt atttctagtt tagattttag agaatttgat gaagtaattc aaattttaac + 1021 tcaaattgtt ttaaagggtc gtcaatcagg ggtcttctta attcttgcca tgcaacgtcc + 1081 agatgctgaa tatctgaaat cagctttacg tgataacttt atgaaacggt tggcggttgg + 1141 tcgtttgact ggttcaggtt atcgtatggt ctttggtgat gaaaatgaga aaaaagtttt + 1201 caaatacatc aagggtaaaa ttggtcgtgg ttatgttgcc aataatggcg aacttgcacg + 1261 tgaattttat agcccttctg taccatttga taaaggctat gattttcatg aagagctttc + 1321 aaaattacca gttttagctg atactactga agttcagctt gaagctccac caatttctaa + 1381 cgttgaacag gaacgagcag aaagtgagct tttggaagaa aaattttata cgattacctc + 1441 tttatctcgc aagttgggtc aaccgtcaaa aactgtgaaa acagccatag agcgtttaac + 1501 aagtggcgga tattctataa gtgaaaaatc accttacaat gaagatgatt ttattgcttt + 1561 acaaactgtt ttcttaacca aagaaacaga agaatgtact ttgaatgaag ccgttgatac + 1621 tgtcttagct gatgaagcag agtttgaaaa agaatttgtg acttttgaca aagaagttgc + 1681 ctagtttgaa aatgaggtaa aaataatggc agaaaaaagt gttagagtaa ctatttcaaa + 1741 ggataataaa acgacggaat tgacccttag aggggttgct gttaaagact ttgaaaagct + 1801 attctttgct gttcaaaatc aaatgaaatc ttgtgaaaaa aaggaagata aacaccacgc + 1861 aatcggcggt gagtaaatga taggaacttg tttccgtttg tctttagaca acttgccgta + 1921 ggcaagccat tttccacggt cgaattgccc tcgcaaacgg tagtgcgcga cagcggttta + 1981 ttcttgaaag aattggagga aaaactattg ggttttgttt taaggctatt gcgttttaaa + 2041 cgtaatgctt tttttgaaaa agtgcaagaa cgtaagcata gctccgagcg tagcgtaagg + 2101 agtgagcgtg tgattgcacg gctgacggct tgccgtcagc ttggacttga ccccccaaat + 2161 gctaatgagg gggggtacaa gtgatacaag tctaactatc ctagttatat caaggttcta + 2221 ggttgtatca cctgtgactt atgtttagaa agtgaggaac gaacgattga attaaaggat + 2281 atacgctcta gcgcaaatgt tactcaacaa ttcatggctg acttactagg tatatcttta + 2341 gtctattatc gaaaaatgga aaatggagac cgtccactct cgaaacagtt tgaagaaaaa + 2401 attagaaatt cttttttcaa aaaaagagag agttcaacgg tctttgttgg tacaaatgac + 2461 tatacaaata ttcgttttca gactttaaat gttcgtgaag tagtttctaa gattttaggt + 2521 ctaaatgttg agaattttca acttaatgag tataaccgtt atcaataccc tttctatatt + 2581 agttacggtc acatcaatgt ttattatcac gataaagata taaaagcagg tgtgctaatt + 2641 gaaatgagcg gtcaagcgtg ccgtgaaatg gaatatgagt ttgaatatca tcaaaaacag + 2701 cgtacttggt acgatttttt taacgattgt tttctctatg ccaataaaaa agcaccagaa + 2761 aatgatgatt ttgtaaaaat cacacgtttt gatttagcac ttgatgaaca atacaaccca + 2821 caagaaggaa attttgactt gttcaaactt ttaacttctg cccgtgaggg gcggtggaat + 2881 ggtcgtaaac aaaactattc tgcggtactt ggtggaagaa gaacaaaaga gggaatgatt + 2941 aatgacggtc taaccgttta ttttggatct aaacaaactc atttattttt tcggttctac + 3001 gaaaaagatt atgagagagc aagtcaagaa atgacttcgg ttgaagcaat acgtgaaatg + 3061 tatggtttgc gaaatcgtta tgaaatttcc atgcgaaaag aaatatcaac cgactttata + 3121 aaaagatata tcgaggaaga ttttgattta gccgatgaag gggtcaaaat tattaatgat + 3181 aatctgacat tttatgataa ggaaggaaac ttagatagtg aatggtacga tatgatgggt + 3241 agaatggatg cttatcattt tactgttcgt cctgaagcgc ctgatttgaa ccgaaaatat + 3301 acatggtttg aacgtggtgg tcctgtatca acttatctat tgttaaaaaa agctgaagaa + 3361 ctgacaggtg aaagtcgttt ggaagaaatt tttaatgaag ctgaactcac agaaagacaa + 3421 gaaaagttcc tgaaggaatt tagaatgata aggggtgcaa atggatagca tattcaaatt + 3481 agcaggtttt tcagttaatg aaatagttta taaagtgtta tttatcatta ttttatgggc + 3541 tataattggt gcaatacttg gtgaaatttt tcagtgataa ttagataagg aggaaaggga + 3601 gaggtgataa aacctaatat agaacatttt caaaaaagaa tgttgagagt tccaaacaac + 3661 gtccgaatgc tccagtataa aatgagtgga tatgaattta atttgaaaaa atttgagcag + 3721 ggagtttatg accagctctt tttttatatt ccaaatgaaa ctgaagcgtt gggattgctc + 3781 aatgaaatag gcttgatgtt tccacctgat tcaatgacac gaaaaaaata ttattcactc + 3841 tatcaagaaa aaaggattgc ggatttgcct gaaggctata aaatggcaat ttatttttta + 3901 attgataatg atttagcaca agccacttta tgatttttca taaataaaaa caaagttatt + 3961 tttcagataa gggaaggcaa attttgtggt gggaaataaa aaaagaaaag agataaaata + 4021 tgccaacttt aaaaacaggc cttaaacaac ttacaactga tttaaacgaa attgaagctg + 4081 taatcaatgc aggaaatgcc tatcaagaag ccgtttcaaa actcaaagga caaggaaaag + 4141 cacttcgtac acttgaaaat gatacctcaa tttcagatga tacagtcgaa gttattgcca + 4201 atcgtacaaa tgatttgatt agaaatcttc tcaaaggttc aactgcatac aactttacaa + 4261 agaaatcatt taatgaatta aaaggaaaac tggaagaagc agaagcgcaa gcacgtagtg + 4321 gcgctaaata aatagtccac gtaaaacttt aaaataaggg gagggtttgc ctttccttat + 4381 ctgaaaaata acattgtaaa agcctcactc atggtaaggc ataagagaaa ggacacctca + 4441 ccatgagtga gaaataaaaa atatgacagt aacaaatatg agcaaagcac gaccacagat + 4501 tgatttttca cttcataaag caccatataa agatttatat gatattcaaa ttttgtttcc + 4561 tgatggtaca aatcaatttt ggtctggaat taatgaaaaa aaggtcatgt ggctgtgtga + 4621 gaaatacaat cctatctcaa ctccgctgtc aattgcgatt gctcctcgat ttttaccaaa + 4681 atctaatatt caaaaaccta attgggaaat tagaaagggt tagaaaatag atagaaacaa + 4741 atatttaaaa atcgctattt attttgatat tttcttagca atttttggac taacttattt + 4801 attaatgaaa ttctttggag gttgaattat gagtgtattt gctgaaagat taaaagagtt + 4861 gcgaaaagaa aaaggactta cacaaagtca agtaggtgtt catttgggaa tgtcacagca + 4921 aaattatcgt aggtgggaag taggcgaacg ttcacctagt ggtgaaactt tgataaagct + 4981 cgcagattat tttgatgtta gcacagatta tttattggga agaaaaagtg aaaaatgaac + 5041 attcaacaac tctatttaat tattaataaa atgaataaag tgcttaaagt tgctagactt + 5101 atttcgctaa ttccagttac gcttattgga ttagcttttg aaatacttac ctctatgttc + 5161 ttatcaggta caatatgttg gtggctggca gcttttaaaa ttatttcaga aaataataag + 5221 attatatttt atctttttag tgtcattatc ttcctcatat tgctagttta tagtttgatt + 5281 gggattctta aagttttaga aaacaaactt aaagagaaag aagttaaatg atgacagtac + 5341 ataagaaaaa actagatttc caatttcata aacccgttga aacgggggaa aaaccttttg + 5401 aagttcgtat aaatgattgt gattatcaag taggcgatat tattgaacta aagtcttatg + 5461 ggaaatgttg taactattgt gatggaaaat catataaaaa atattattca ggaaattgta + 5521 gcggttggat gaattgctat gaatctgaag ctgaaactat cagagtaaag atattaagag + 5581 ttatttatcc taaagaaatc aattatgctt taacgcatgg ggttatttta tcaagcagtt + 5641 tcggtaaaat aaatcttgat gatgttagag aagtattgaa agaatacttt aacagaaaaa + 5701 cgttacctaa agattatgta cttcttgaaa ttgaggttat taaaaaataa ataaaacttg + 5761 tccgaaacga caaaaaacta gtcgcttaac tgtcagaaat ggcagttttt ttattttagc + 5821 taaattaaaa aaaggagatt tactcatgag taaacacaaa aaattgttaa atggcttaac + 5881 ttctgaaaag aaagttacca attggaaaat gcacaaacgt aaaaaagtat ttgttgcctt + 5941 tgcaggagtt ttggctgtgg gtttgggtac agcaggactt gtatatgcta gtgcacaaag + 6001 tacaagtgcg aaggctagtt taccaacaga tacaacggtt aactgggaaa ataacaagcc + 6061 tttgtattat gaaatcgacc aaacaggaaa agaacaccca aaacctatgc ttaaactatc + 6121 agatggaaca cctgcatggt gtttgggtct tggtgtgcct ttggttaaca acataactca + 6181 agctcaatta gattctagta atgcaatttt aaatgcctta tctgatgaac agattgctgt + 6241 gattaacaat gtggcatatt tagctcaaaa acaaggaacg ttgttggcat atgcacaagc + 6301 tcaacatgcc acataccttt tacttgatga agcaggggta tctgtcaatc agacaaaaga + 6361 tttgattgta aaaccaaaca ctttattaca agaacctaat gccattaaaa caggcgcaaa + 6421 tgacttgatt acacaagcaa aagctcttcg taaattgcca agttttcatg gaacaacggt + 6481 agatgtggtt caaggggtag aaaaaacagt tactgattca aacagtgttc ttgctaattt + 6541 tccaaatttc aaaagtaatg tttctggttt atcagaaagt gtttcaggca atgatttgaa + 6601 actgaaagct gatattacgg caaaagttgg aatcactcca aaagcaattc aattccaaaa + 6661 cacacctctt tcaattgctg atttacctta ttatgtcttt tcgactgatg gagatagtac + 6721 agggaaagca agtcaatctg tgattgcttc acaagaccct tcaaaagcta atggttatct + 6781 aaacgttaat gttattggtc ttggagaaac aacattgaca aaagtggatg ccgatactgg + 6841 cagtggtgaa acacaaggag ctgcacaatt aaaaggggct gtctttggtt tgttcaataa + 6901 atcagacaat tcacctgtga aatggtctga cggtcaaaaa ggatatccta ttacagttac + 6961 tgctggtact aaagcagatg atactaatgt ttccattaag cttggtgatg acttgaaagc + 7021 aggattgaaa aatcttgata actcaaaaga gtattattgg aaagaaaccg ttgctcctga + 7081 aggttatagc ttatcaaccg aaaaatatga agtaaaattt gattcttcaa gtaaatttga + 7141 tgataaaaca tcaaacttta tagatgatga taaggcaact gataaagtcc ttgacttaaa + 7201 ttttggcttt atcaaagcgc aagatgtcaa cggttcattg actggcttaa atggtcgtac + 7261 tttccgatat actcctacag gagatactaa aggaaaacca attgaagtga cttcaggagc + 7321 aaatgaagat tctaacggtg taacgaacaa cggacaagtt aatttcacca aaattccttt + 7381 tggtgatgga cttcttgaag aactcccaca aaaagatgat aaacttcaat taatcaaccc + 7441 tatctctatt gttacgacaa ctaacaagga taaggatgaa aatatcacag gttatacagt + 7501 cacatttact gatactgtta caaaacaagt gattacaacg cttgatgttt cgttggataa + 7561 agtaactgat aattctacaa tgttcaaagt taatcttgga actttggtag ataaaccagt + 7621 tactcctgtc gtgccaacca ttaaaacaaa ggcacatacc aaagatggcg accaaacgat + 7681 tcaaaaagtc gaaatttcca aaacaactcc tctctacgat aaaatcatga caacaaatgc + 7741 tataaaaggc gaccaaatgg tggctacact tcaccgtgtt gtgacgggta aagacggaaa + 7801 agtgacagaa tcaaaagtta tccgtacttt gaatttcacg gttgatgatg aaacggtcaa + 7861 agcacaagaa aaacaaattg aatctactgt tgatacaacg aaagacgctg atgttcctga + 7921 aacttcaact gtagcctatg tttggactga agatttattt gatgaaggaa aaaatcctaa + 7981 aacagatgaa ccaaaagcaa aacatgatga tttgaaagac caagaccaaa cattgaccgt + 8041 cgaaaaagtt gaaaaaccag ttactccacc aagttcaaaa cctactccac caagtaccac + 8101 tcctacagaa actcctaaag tttcattacc aactacagga agtgaaacag gcgatatgat + 8161 tatgtatggt ggtgcattag ccgtcttaat cgctcttggt actggtgctg tttattatat + 8221 gaaaaagaaa aaatcaacta aagaagaagg gtagaaaaga gcagacttga tgtctgcttt + 8281 tattttgaat aaaaatgaaa aatacaaaat ataaatcaag aactccacca aataaatttt + 8341 atgaagtgtt atttcaaatt tataaataag agaggaaaaa atgtacgttg taaaaatgcg + 8401 tggtggctat ctttgtgcca atggtggagc gactaaacac ttaaaatttg ctaccacttt + 8461 tgatactaaa aagaaagctg aagaagtggc tgaaaaatgg ttaagaagtg atgtatcttt + 8521 taaagctgtt gaaaaagaaa gtgaggaata tgagcagaat aaaaattagt gagttggcag + 8581 atgaattcaa aattgacaga aaagatgtgc tggctaaagc gcaggaattt ggggtttcag + 8641 ctaaattggc aacgaaaacc ttgagtgaat cagaagaaaa acagttgaga acatattatc + 8701 aacaggttcc acattcaatg gctgaaacaa gcaaaaatca agttttagaa aatgtaggac + 8761 agaaaaagct tgaccctaaa gaagaagttt cagaaaaaaa agaagaaccc tctaaagcag + 8821 aacaagtgga aaaaaagaaa tcaaaatttt caatattggg tcataaaaag tctgaaaaga + 8881 aaaaggggaa actgctcact cgcctttcaa atgaaaaacc accgtcaaaa aagcagttta + 8941 ctgcaaagca gggtggggca ttagttgtgg gtggggttgg aatcttaata ttatctaaca + 9001 tggtcttgtt tggtttaatg gcaagtggtt atcagccgac acaaaaaatc attcatgaac + 9061 aggtaagtgc cagtcaaaaa gtttcaggaa atggacttga tttacaagcg aaaaattatc + 9121 ttgatggctt tgttcaaacc tactttactt ttcctgaaaa tgaaaaagac caagaacaag + 9181 cggtcaaaga tattaatggt tactttgttc aacaattgcc agtcattagc caagggcttc + 9241 aacgttcacc ttcaaagttt gaagaagcgg tattaatgaa tttaaccgat aatgaagcca + 9301 cttataaagt gacttatggg gcaggggcag tcacgaaaaa gggaaaatca aatgtggtga + 9361 attatcatga tgtcacaact ttatttacta ttccttatca aaaagtaggg tcaagttatt + 9421 atgtttctga tgaaccttac ttttcaagtg ttaatgattt acaagctaaa gaaagtcaag + 9481 tccctgtcaa aacatggtca ggaacagata ataatagcgc ttcgactaag aaagacttag + 9541 ataagttcac aaaagcttta tttacggctt atacaactga tagtgatacc ttaaagctga + 9601 tttcagaagg gttatctttg aatgatagcg aaacgttcca gtctttagac caagccactt + 9661 atgaacctaa aggtgacaca tatcaagcta ttgttcaagt gacaatgaaa aatgagctag + 9721 gtactcatgt agaaaactat caatttacaa tttcaaaaca aaagcagtct tattttgcga + 9781 ctgactttaa gcatactttg ccaaaaggca ataaagaata ggagaaatta aaggtgaaag + 9841 aattattaaa ttatcatatt gccgttgttt atggagatat tggactttct aatttttgga + 9901 atacatttca aaccaatgtg aaatatatta tttttgcagg gactttatgg tttatgataa + 9961 gggaatggaa aaacaaagcg gttggtaaaa tggtaatgac ttttattgtt ggggctattt + 10021 tatttgcatt aacattgaat ccagaaactg tccttcaacc aataggacaa aagttaatgc + 10081 aaatgattgg ggcgtaatta aggagcgaaa atgtcaaaag aaaataaaaa aggactgaaa + 10141 tattcaaata aaagttcact ttcacaaccg ttaagaatcc aaaagctttt tgatggatgg + 10201 tcgctaggca aggcttggcg gttgtccttt tttatttact ttggattgtt tggctttatt + 10261 ttatggaaac ttttattttc aaaattagtg gtttttccta ttggctttcg attggtcgtt + 10321 gagagcttta tgtgttataa aagcgcctta tttttagcgg aaatacggat tgatgataaa + 10381 tcgccgttta tttatctaaa agatatgata agtttcatta taacttttgg cttgaaagga + 10441 aaaagcattt ataaagggtg gatttaccct ttacgcaaga gaaaagagga attaaaagaa + 10501 aaatgaagaa aaaagcgaca tgggaaaatc atattttatc agttcatgaa aatttggttt + 10561 taaaagatga tggcacagtt tttgccatgt ttgaagttcc agcttcaatt atttcaatga + 10621 tggatgaaaa aggaaagaaa aaccataaac aaacaacaca agcggtgatt accagtttgt + 10681 atgaacattt aggctttgaa attttgatga agcctatcac aaaggattta ctcaaagcgt + 10741 acggctattt actggaagat tgtgacccac gaacacgaga gtttggggag tatttattag + 10801 aacaatctta tcttgaatta gaaaatgagt tagggaagat ttacaatcat aaattctttt + 10861 taactgttcc attgagtgac tttgcttttt caggcgattt gaaaaagatg atgaaagaag + 10921 ggtggcaaag atttagaaaa tcagtcgttc aattgattcg ccgtgagctt gaatttacgg + 10981 tcaattggta tgaagattat gaacaaacgg ctttagattt agaaatgaat ttaaatctcc + 11041 ttcaagctcg tccattaaaa caagaagaaa cactgcttta taattcctta aattacttgc + 11101 gtggcattga tgtcaatcaa gatgaacttt tggctgatgt gaacaatgcg attgaaaata + 11161 ttgatgatac aacggttgat gttcgctcag atggtttgct tgaaattcat catccccaag + 11221 gagagagcct gttaaaattt ttgcccctcg ctgattaccc tgatgtggtc aatgatattc + 11281 atttaataga acatattcaa accttgccct ttccagttga atttcgtgtc aaggctcgtt + 11341 ttagaaagaa caaaggggct ttaggaatgg aagcaacagc agaaagaaca cgtgaccgaa + 11401 taggaacgga actttctgaa gcagatgagc aaggcaatgt gactaaaaac tcatctgtga + 11461 gtgcctatgt gattcttgaa gatattattg caggggtgga tggtggggaa tatttcttag + 11521 actttttacc tgtttttgcg attactggtt caacgcctga tgaaattaat tattataaaa + 11581 aaatgttaat gacggcaatg gaaggtttag gaatcaaaat tgttcctagt caatgggatc + 11641 aaccctatct attttataaa atgcgctcaa cggaagagtt gacccgttcg gataagtatt + 11701 gggtgcaaaa tatgaaagtt tcggcatttg ttgaaaattt attctttgtg agccaaaagg + 11761 ttggttcgga tgttgggttt tattttggac gagttgacca tactcaactg aattgggcag + 11821 gaaattttga aaaagccatc gctagctcac ctactccttt tttctggaat atgtttcaag + 11881 ccaataagga cgatattgaa gggaaattgg gagatagtcc tcacgtgggt atttttggtg + 11941 atacaggctc agggaaatct ttttttgcca aacaaggatt tgttaatcat tctatcttaa + 12001 aaggattgag cctttatatt gaccctaaag atgaaatgaa aagtcaattt ttagctgttc + 12061 gagataaatt agaatcttat ctgcctgaaa tggaagaaaa aatttctgaa ttagtggttc + 12121 tcattgaaga tgatgagatt ctagctcgaa gaattaggaa tttagagttt tcttatcata + 12181 aaccactgat tgattatatt aatcatattc attttgtcag cttggatgtc aaagataaga + 12241 aaaacatcgg agcgcttgac cctattgtct ttttagaagg caatcaagcc acagagcttg + 12301 ccacgcaagt cacaaaaggt ttaattggcg ataagttaac agaagatgac cgctttgaga + 12361 attgtttcaa tcaaaaagtt caagaagtga ttgaagccag agccaaagga aaaacggtag + 12421 gacttttaaa tgtttttcaa ctcatggcaa aatcagaaga agattttatc agataccgag + 12481 cagaaaatat tttatctaaa atttcaggta cgatgttaga gctggtcttt tcagaaggtc + 12541 aaaattcctc agtcagtatg gatgaacata ttactatttt aggagtgaca ggcttaaatt + 12601 tagcgaaatc aggggaagtc aaaacagctc aaaatgaaat gtctgatatt atcatgtatg + 12661 ccttaggtga tttttgtcgg ttctttgggg cgagagatag agaacaggaa acggtcattt + 12721 ggttagatga aggttggttt tttaatacaa cagatattgg aaaaggaatt ttaatgcgaa + 12781 tgaaacgtgt cgggcgttca gaaaacaatt ttttagtttt gattagccaa agtgtgaaag + 12841 atggtgcatc agaagaagat gatacggcat ttggtacaat ctttgccttt aaagaaactg + 12901 ggaacacaac ggcagctctt aaagcgattg gactgcctga tgatgtggaa gaaatcatta + 12961 aatggtatga aaatatgaca aaagggctgg cgcttttgaa agacccattt ggtcgaattg + 13021 gtcgtgtggt cattcaaggt caaaattcag cgttgaatga atgctttaaa acctcattta + 13081 gtaaaatgaa atcaacgaag gcggtggcat aatgaaaaag aaaatgttgt tagtggtagg + 13141 gctgttggca tttttaggag gggttgccct acctagtcaa gcggatacgg cttttaacgt + 13201 tccaaccact cctagttttt cgagtgctaa aaatccaaca gtttcaaaag cccctacttc + 13261 atcatcttca acggcttcaa gcaaaagctc aaccacaagt ccttcttatc cagttaacaa + 13321 tgggtttgtt ggtggggatt tgggtgatta ttcttcgtct aactctccaa gtaaagtaat + 13381 gaatgaagta gagaagaaaa aagcgaggga aaatttagat aattatacct cttatatgta + 13441 ttttgatgat ggaaaatttc tcacgggctc aatgagtgct gggatgcaga ccttttttgt + 13501 tcgttctcaa tttttcgtca caaaaaccat ttatcggttt gtgaatgcgg tcaatgaaaa + 13561 actcaatagt gattctttga ttacacaatg gacagcgcaa atgtttgcga cagtaaaaaa + 13621 aatttacaat caattttcta accctcaact ttacccaatc attgccattg gcgttattag + 13681 tagcttattg tattattggt ttaaacgtcg ttttttagaa ggagtgagaa aagtcatact + 13741 ggtcatgtta ttagtggggc tttttatcaa tgggggtcaa aaactaactg aacaaatcaa + 13801 ttcatcattg aatacagcta caactacgtt gatgtcaacg gttaaggtgg caggcgtttc + 13861 ttcaagaaat tcaaatgatt taaaaacaac aatggtcgaa gtcccttttc tttatatgaa + 13921 ttttgataat gtcaaaatca atgcggatgg ttcatcaaat atttctgagg ataatattgt + 13981 aaaacttctc acctctgatg atgacaatga taagttaaaa acgattcaat ctgatttaaa + 14041 agattcacat ttgacctcga aaaaaatggg cgaaaaagta ttaactgctc tagccagtat + 14101 tttcaatgcg atattagttg gttttattta cctagctttt gcggtgatgg cttttgtcat + 14161 gcgaatgttt ttcttaattc tcttactttt actacccttt gtggggattc tttccttatt + 14221 tcctttcttt gatgtagtga ttttaaggtg ggcaaaagca acaggtgggg cgttgattgt + 14281 ttctaatgtg gtcgttattg ggacagcttt aattagtatc ttagatagta ttgtgtcttc + 14341 tgcggtaact tcaatgattg ggagcgatta tttctttatt acactcatca aatttattgt + 14401 ttatattatc ctctttaaaa agcgtgaaaa gattcttgaa atatttaagg caggacatct + 14461 ggggaatagt ggctttgcag ggcgtatgga tggcttactc tcaaatgtgc ggagaaaagg + 14521 ctcaaatatg attaaagctc cacttctagc aggttcttca gcaggactgg tcgcaggttt + 14581 aaccgcaggt caaatggcaa ctggtaaggc gaaagaaatt gctaaaaatc gtttgacaaa + 14641 cggtacaggc agtttatggc atggtggttt gaataaaaaa gcagaccgta ccatgaataa + 14701 aatggataaa gcaaatccta atagtaaaaa aggtcaaaaa ttacaggcta aagaagaaaa + 14761 aatcaaacaa cgtttggaaa aacgcaaaca gaaatttgaa aatcctaatc tgctaaaacg + 14821 aaaagtagct gactatcgca aacagtatca acaacgaaaa actatgaata acccaaatct + 14881 tgctcaaaag atggaacaaa aaaataaaga aaaaaatgct tcgattcaag ctctttatga + 14941 taaaaataaa actcaacttc aagcaagagc aattgagcgt agaatgcgtg aagctgttcc + 15001 tgaccaagaa aaacctaatc tatcagaatt agctaaaaat aagcaaaaag ctttagctaa + 15061 agaaaagtta gatgaaagag caatgaaaca atcagcaggt agaatggctg aaagatttga + 15121 aaaggaaaaa gttagtcata gtagttttaa acgtaaacca aactatcctt gggataataa + 15181 tgataccaat caaaaactta acaatccatt tgtgattgat gaaaaatagg gggtgaaaat + 15241 gaaaaaagca attaagcgga aggttatatt agttagcctt ccttttttgt tgcttttatt + 15301 gccaatatta gctttctttt cattatttgt tggttcaaat tcttcttcag atacagatat + 15361 taataccaat acaccacagc aacaaacagc aaaagtgatt tgggatagag ttctaaaaga + 15421 aggtggaacg aaagaagggg cggctgcttt acttggtaat aatcaagcag aaagtgaact + 15481 tcaaccttca attattcaat ctaatgcaac ttataatgaa gcaaaagcaa tggataccac + 15541 tttaggcggt tatgctttcg gtttggctca atgggatagt ggcaggcgag taaatttgct + 15601 taactatgca aaaagtcaga agaaatcttg gacagatact aatcttcaag tcgagttcat + 15661 gtttgagcaa gacggtacag attctacgtt gcttaaacaa ttaattaaag gaactaatgt + 15721 taagcaaaca actgaagata ttatgcgaaa gtgggaacgt gcaggggcgg ttgatagcct + 15781 ttcaaaacgt caaggttttg cggaatattg gtacacgttc atgacgactg gtggtgatag + 15841 tggaactggt ggcggttcag gtattactcc agatatacct tcaggttgga ctttggataa + 15901 accaattaac acaagcggtt atattacttc aagttatgaa tataaacaat gtacttggtt + 15961 tacgtggaat cgagcgaaag aattcggtat tacttttagt ccatttatgg gaaatggtgc + 16021 tgattggcaa catcaagcag gatatactgt aacgacaact ccaacactcc atagtgcggt + 16081 tagctttagc ggtggtcaaa cagtaggcgg tcaatggaac gcagacccag tttatggtca + 16141 cgtggctttt gtggaagcaa tacattcaga cggttcagtc ttaatttcac agtcaggaac + 16201 tggttttagt acggtttata ctttccaagt gctgacaaaa gcacaagcca gtcaattaca + 16261 ttacgtgata ggaaaataaa aaatagtcga ttaacgttcg gtattagcct agttttcacg + 16321 ttgaaaattc cagggttttg aaagttattt ccgaacaatg gaaaggagag agtatgacta + 16381 aacttgaaga agttagagca agtgggaaaa tgtcagaaag ggtattagag aataatttta + 16441 gacactttga ccacagactg cgagaaatag aaggagaatt aagactttac ccttatgcga + 16501 ctttatctga agttatagca tgggctgaac aactaaaaat agcaattggg aaaattaaag + 16561 caatacaaga aagtagtatt ataaaatcta aaaaagaatg gggaatctta gaagaaaaaa + 16621 tgttgggtta tttgcagata gacaaagcat ttattcatgt attttctgac catgttattt + 16681 tccttgttca attggaacag cggtatcggc aaagattaag tatttttgcg aacaatttag + 16741 ataatagtgt tcgctatcta aaaagatatg cggatgattt agaaaagcaa ggattttcta + 16801 taactggaat acttgctgaa agtagaaatt taagtgatat gaactggttg agtatcttaa + 16861 attattaatt tagaaaatta tgagggtata aaaatggaaa caccaaaaat ttatgttgta + 16921 aatttaaaca gttataacaa tggaagaaca agggggaagt ggtatgaatt acctgttgat + 16981 ttttcaagaa ttaaatcaga tttactttta gatgtagagc atggggaaga atatgcaata + 17041 catgactttg aaaattttta cggttataaa gtaggcgagt attcgtcaat tcaggaactt + 17101 aatgagtatg cggaaaaatt agaagaaatt tctgacatag aacatttaaa agattttctt + 17161 gaaatttata gtattgatga tgttatcaat aataaagatg atttggactt tgtggaagct + 17221 gaaaatgatg aagatttggc acaagaatta attgagcaaa tgggcggttt agaagttcta + 17281 agcgtagaaa cgttacaaag atattttaac tttggtgctt atggtcgaga tttagcaatt + 17341 ggtgattatt caaaaacaag tcatggatat attagagata tttaaaggtt aagtaaaaga + 17401 gcaagaaaaa ccttgctcta ttttttgttg gaggaaaaaa tggaagaaaa ttataaagat + 17461 aaacggggaa tttcaaaatt atttgatgta cctataaaaa cacttaataa tgatttaact + 17521 gaaatgagac gaacagaatt taatgtttat atattacgac caagtcataa acgtgtttat + 17581 ataaatgtcc agggttataa atcttttctt gaatataagc aaaaaataag agaaagtaca + 17641 atttagaaaa aagataaaag taatatacag atatgatata atataagtgc tgtatagctt + 17701 tcttttatct ttggtaaaag gaggaaatat gttttataag caacttgata gtggaaaata + 17761 tagatacttt gaaaaatatt tcgatgaaaa gagaaataaa tggcgacaag taacggtcac + 17821 tttaaaatca aaatcaaggg ttgctcaatc agaagctaaa aatagattag caagaaaaat + 17881 tgaacaatca aaaaaagtac caactgctat cgagcttcag gaacaaatcg ttcaaaataa + 17941 gacgatacaa gaaatttatg aagaatgggt tgtaattaga aaacaagatg ttaagccagc + 18001 aagttttgta gcggagcaaa tttctttaaa aggatttatc gaaaaatttt caaaatataa + 18061 agtctcagaa gtgacgaccg cagatataca aagttattta atggaattag atattgcgaa + 18121 ttcaacaaga aaaaatcgta gaatttacat tagaattttg ttcaaatatg ctgaaaacat + 18181 cgggtatatt aattcaaatc cagctgacaa agtagtatta cctaaagtaa ggttagaaat + 18241 tgaaacatta gaacgagcaa atgaaaaatt tttaagtaaa gaagaaatga gttctgtttt + 18301 gatattttgc aaatcttata aaaaaaatat aagatacact ttagctatgg aatttatttt + 18361 cctaacagga tgtagattcg gtgaatttgc ttctattcgt tatcaagatg ttgatttcaa + 18421 aaataagtta ctaagaattg accacacttt agaatatcgt gttgcaaaat atgatgatcg + 18481 agttattcaa acacctaaaa cggtaggttc gattcgtaca attagtttaa gtaatcgttg + 18541 cttggaaatt attgattatt tccaaaaaaa ctgtttagat gataagtttg tttttgtaaa + 18601 tgcggttggt ggaattttca gacaacctgt attttataag tttatttgtg ataattgtca + 18661 aaaagtatta ggaaatgaaa gaaaatacgg tatacattta ttgagacatt ctcatgtatc + 18721 attacttgtg gaacttggag ttccaattaa agcaattatg gaacgagttg gtcatagaga + 18781 cgagtcaatc actctgagga tttattctca tataagtggt acaattaaaa atgaaattag + 18841 tcaaaagttg aaccaaatta atctctaaa +// +