Mercurial > repos > iuc > meme_psp_gen

annotate meme_psp_gen.xml @ 2:b48e673af4e8 draft

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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit e2cf796f991cbe8c96e0cc5a0056b7255ac3ad6b
author iuc
date Thu, 17 May 2018 14:11:15 -0400
parents a0fa4efeeee3
children e24560178fad
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1 <tool id="meme_psp_gen" name="MEME psp-gen" version="@WRAPPER_VERSION@.0">
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2 <description>- perform discriminative motif discovery</description>
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
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6 <expand macro="requirements" />
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7 <command detect_errors="exit_code"><![CDATA[
2
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8 ln -s '${primary_sequence}' meme_psp_input_pos.fa &&
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9 ln -s '${control_sequence}' meme_psp_input_neg.fa &&
0
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10 psp-gen
2
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11 -pos meme_psp_input_pos.fa
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12 -neg meme_psp_input_neg.fa
0
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13 -minw $adv.minw
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14 -maxw $adv.maxw
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15 $adv.alphabet
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16 #if str($adv.triples_cond.triples) == 'yes':
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17 -triples
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18 $adv.triples_cond.fixedstart
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19 #end if
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20 $adv.equiv
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21 $adv.revcomp
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22 $adv.scalemin
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23 $adv.scalemax
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24 $adv.maxrange
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25 $adv.raw
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26 #if str($adv.report_scores_cond.report_scores) == 'yes':
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27 -reportscores
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28 $adv.report_scores_cond.verbose
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29 2> '$output_tabular'
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30 #end if
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31 > '$output_psp'
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32 ]]></command>
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33 <inputs>
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34 <param format="fasta" name="primary_sequence" type="data" label="Primary sequence file"/>
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35 <param format="fasta" name="control_sequence" type="data" label="Control sequence file"/>
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36 <section name="adv" title="Additional Options">
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37 <param argument="-minw" type="integer" value="4" min="0" label="Minimum width to use for position specific priors"/>
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38 <param argument="-maxw" type="integer" value="20" min="0" label="Maximum width to use for position specific priors"/>
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39 <param name="alphabet" type="select" label="Alphabet">
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40 <option value="-dna" selected="true">DNA</option>
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41 <option value="-protein">protein</option>
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42 <option value="-rna">RNA</option>
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43 </param>
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44 <conditional name="triples_cond">
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45 <param name="triples" type="select" label="Use spaced triples instead of whole-word matches?" help="Recommended for protein">
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46 <option value="no" selected="true">No</option>
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47 <option value="yes">yes</option>
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48 </param>
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49 <when value="no"/>
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50 <when value="yes">
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51 <param argument="-fixedstart" type="boolean" truevalue="-fixedstart" falsevalue="" checked="False" label="Allow triples to start anywhere within a site?" help="Select 'No' to only consider triples starting at the start of the site"/>
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52 </when>
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53 </conditional>
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54 <param argument="-equiv" type="boolean" truevalue="-equiv" falsevalue="" checked="False" label="Match as equal sequences of letters that appear together?"/>
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55 <param argument="-revcomp" type="boolean" truevalue="-revcomp" falsevalue="" checked="False" label="Consider both strands when calculating position specific priors for alphabets?"/>
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56 <param argument="-scalemin" type="boolean" truevalue="-scalemin" falsevalue="" checked="False" label="Set the lowest score value after scaling?"/>
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57 <param argument="-scalemax" type="boolean" truevalue="-scalemax" falsevalue="" checked="False" label="Set the highest score value after scaling?"/>
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58 <param argument="-maxrange" type="boolean" truevalue="-maxrange" falsevalue="" checked="False" label="Choose the width with the biggest difference between minimum and maximum scores before scaling?" help="Select 'No' to choose the width with the biggest maximum score (before scaling)"/>
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59 <param argument="-raw" type="boolean" truevalue="-raw" falsevalue="" checked="False" label="Output scores instead of priors?"/>
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60 <conditional name="report_scores_cond">
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61 <param name="report_scores" type="select" label="Output primary and control file names, scores and widths?">
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62 <option value="no" selected="true">No</option>
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63 <option value="yes">yes</option>
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64 </param>
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65 <when value="no"/>
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66 <when value="yes">
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67 <param argument="-verbose" type="boolean" truevalue="-verbose" falsevalue="" checked="False" label="Report frequency of each score?"/>
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68 </when>
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69 </conditional>
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70 </section>
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71 <param name="non_commercial_use" label="I certify that I am not using this tool for commercial purposes." type="boolean" truevalue="NON_COMMERCIAL_USE" falsevalue="COMMERCIAL_USE" checked="False">
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72 <validator type="expression" message="This tool is only available for non-commercial use.">value == True</validator>
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73 </param>
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74 </inputs>
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75 <outputs>
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76 <data format="memepsp" name="output_psp"/>
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77 <data format="tabular" name="output_tabular" label="${tool.name} (report) on ${on_string}">
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78 <filter>adv['report_scores_cond']['report_scores'] == 'yes'</filter>
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79 </data>
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80 </outputs>
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81 <tests>
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82 <test>
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83 <param name="primary_sequence" value="meme_psp_protein_input.fasta" ftype="fasta"/>
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84 <param name="control_sequence" value="meme_psp_protein_input.fasta" ftype="fasta"/>
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85 <param name="alphabet" value="protein"/>
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86 <param name="report_scores" value="yes"/>
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87 <param name="verbose" value="-verbose"/>
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88 <param name="non_commercial_use" value="NON_COMMERCIAL_USE"/>
2
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89 <output name="output_psp" file="meme_psp_output_test1.memepsp"/>
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90 <output name="output_tabular" file="meme_psp_output_test1.tabular"/>
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91 </test>
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92 </tests>
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93 <help>
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94
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95 .. class:: warningmark
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96
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97 **WARNING: This tool is only available for non-commercial use. Use for educational, research and non-profit purposes is permitted.
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98 Before using, be sure to review, agree, and comply with the license.**
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99
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100 psp-gen is used to allow MEME to perform discriminative motif discovery—to find motifs overrepresented in one set of sequences compared to in another set.
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101 It takes two files as input—the sequence file to be input to MEME, (the "primary" file) and a "control" sequence file of sequences believed not to contain
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102 the same motifs as in the "primary" file. psp-gen creates a file for use by MEME that encapsulates information about probable discriminative motifs. psp-gen
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103 records its chosen motif width in the file, and MEME is able to adjust the data when it tries different motif widths.
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104
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105 .. class:: infomark
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106
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107 For detailed information on psp-gen, click here_, or view the license_.
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108
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109 .. _here: http://meme-suite.org/doc/psp-gen.html?man_type=web
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110 .. _license: http://meme-suite.org/doc/copyright.html?man_type=web
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111
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112 -----
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113
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114 **Required options**
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115
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116 * **Primary sequence file** - a file containing FASTA formatted sequences which are to be used as the primary set in PSP calculation.
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117 * **Control sequence file** - a file containing FASTA formatted sequences which are to be used as the control set in PSP calculation.
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118
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119 **Additional options**
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120
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121 * **Minimum width to use for position specific priors** - the minimum width to use with selecting the "best" width for PSPs.
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122 * **Maximum width to use for position specific priors** - the maximum width to use with selecting the "best" width for PSPs.
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123 * **Alphabet** - The alphabet to be used, one of DNA, protein or RNA.
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124 * **Use spaced triples instead of whole-word matches** - use spaced triples instead of whole-word matches (recommended when using the protein alphabet).
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125
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126 * **Allow triples to start anywhere within a site** - when using the -triples option, select 'Yes' to only consider triples starting at the start of the site or 'No' to allow triples to start anywhere in a width 'w' site.
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127
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128 * **Match as equal sequences of letters that appear together** - select 'Yes' to match as equal any sequence of letter that appears together. Separate letter groups using "-" (e.g. -equiv "IVL-HKR") means treat all occurrences of I, V or L as the same, and all occurrences of H, K or R as the same.
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129 * **Consider both strands when calculating position specific priors for alphabets** - select 'Yes' to consider both strands when calculating PSPs for complementable alphabets or 'No to consider only the given strand.
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130 * **Set the lowest score value after scaling** - select 'Yes' to set the lowest score to 0.1 unless the the following "highest score" option is selected, in which case the lowest score is highest score - 1.
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131 * **Set the highest score value after scaling** - select 'Yes' to set the highest score to 0.9 unless the previous "lowest score" option is selected, in which case the highest score is lowest score + 1.
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132 * **Choose the width with the biggest difference between minimum and maximum scores before scaling** - select 'Yes' to choose the width with the biggest difference between minimum and maximum scores before scaling, or 'No' to choose the width with the biggest maximum score before scaling.
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133 * **Output scores instead of priors** - select 'Yes' to output scores instead of position specific priors.
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134 * **Output primary and control file names, scores and widths** - select 'Yes' to produce an additional tabular output consisting of control file names, lowest and highest scores and lowest and highest widths.
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135
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136 * **Report frequency of each score** - select 'Yes' to include the frequency of each score in the output.
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137
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138 </help>
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139 <citations>
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140 <citation type="doi">10.1186/1471-2105-11-179</citation>
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141 </citations>
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142 </tool>