Mercurial > repos > iuc > miniasm

annotate miniasm.xml @ 3:73a3846701e1 draft

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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/miniasm commit 5b819c9a19a38ebdd60bcd0ea3f530bf60a688bb"
author iuc
date Fri, 02 Apr 2021 20:20:44 +0000
parents 8dee0218ac7d
children a7b889b90140
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73a3846701e1 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/miniasm commit 5b819c9a19a38ebdd60bcd0ea3f530bf60a688bb"
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1 <tool id="miniasm" name="miniasm" version="@TOOL_VERSION@+galaxy1">
0
8b6d0d85252b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/miniasm commit 4e244b45154a712c786f6bca774e0b25676f4642
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2 <description>Ultrafast de novo assembly for long noisy reads </description>
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3 <macros>
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2dcd07f40204 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/miniasm commit 7426f5bb77ff445f5f87ab3bdc3febdff7b55fa7"
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4 <token name="@TOOL_VERSION@">0.3</token>
0
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5 </macros>
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6 <requirements>
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7 <requirement type="package" version="@TOOL_VERSION@">miniasm</requirement>
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8 </requirements>
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9 <version_command>miniasm -V</version_command>
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10 <command detect_errors="exit_code"><![CDATA[
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11 miniasm
8b6d0d85252b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/miniasm commit 4e244b45154a712c786f6bca774e0b25676f4642
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12 -f '$readFile'
8b6d0d85252b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/miniasm commit 4e244b45154a712c786f6bca774e0b25676f4642
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13 -m '$preselection.minMatch'
8b6d0d85252b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/miniasm commit 4e244b45154a712c786f6bca774e0b25676f4642
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14 -i '$preselection.minIden'
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15 -s '$preselection.minSpan'
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16 -c '$preselection.minCov'
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17 -o '$overlapping.minOvlp'
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18 -h '$overlapping.maxHang'
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19 -I '$overlapping.intThres'
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20 -g '$graph.maxGapDiff'
8b6d0d85252b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/miniasm commit 4e244b45154a712c786f6bca774e0b25676f4642
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21 -d '$graph.maxBubDist'
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22 -e '$graph.minUtgSize'
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23 -n '$graph.nRounds'
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24 -F '$graph.finalDropRatio'
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25 '$paf' > '$gfa'
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26 ]]></command>
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27 <inputs>
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28 <param name="readFile" type="data" format="fastq,fastq.gz,fasta,fasta.gz" label="Sequence Reads" />
0
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29 <param name="paf" type="data" format="tabular" label="PAF file" help="Pairwise mapping format" />
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30 <section name="preselection" title="Preselection options">
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31 <param argument="-m" name="minMatch" type="integer" value="100" label="Drop mappings having less than this number of matching bases"
8b6d0d85252b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/miniasm commit 4e244b45154a712c786f6bca774e0b25676f4642
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32 help="column 10 in PAF"/>
8b6d0d85252b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/miniasm commit 4e244b45154a712c786f6bca774e0b25676f4642
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33 <param argument="-i" name="minIden" type="float" value="0.05" label="During read filtering, ignore mappings with col10/col11 below this value"
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34 help="Ignored mappings are still used for read overlaps" />
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35 <param argument="-s" name="minSpan" type="integer" value="1000" label="Drop mappings shorter than this number bp"
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36 help="This option also affects the second round of read filtering and minimal overlap length"/>
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37 <param argument="-c" name="minCov" type="integer" value="3" label="Minimal coverage by other reads"
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38 help="In the first round of filtering, miniasm finds the longest region covered by this number or more reads. In the second round, it in addition requires each remaining base to be covered by this many bases at least minSpan/2 from the ends of other reads" />
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39 </section>
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40 <section name="overlapping" title="Overlapping options">
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41 <param argument="-o" name="minOvlp" type="integer" value="1000" label="Minimal overlap length [same as minSpan]" />
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42 <param argument="-h" name="maxHang" type="integer" value="1000" label="Maximum overhang length"
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43 help="An overhang is an unmapped region that should be mapped given a true overlap or true containment. If the overhang is too long, the mapping is considered an internal match and will be ignored" />
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44 <param argument="-I" name="intThres" type="float" value="0.08" label="Minimal ratio of mapping length to mapping+overhang length for a mapping considered a containment or an overlap"
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45 help="This option has a similar role to MaxHang, except that it controls the ratio, not length" />
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46 </section>
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47 <section name="graph" title="Graph layout options">
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48 <param argument="-g" name="maxGapDiff" type="integer" value="1000" label="Maximal gap differences between two reads in a mapping"
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49 help="This parameter is only used for transitive reduction" />
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50 <param argument="-d" name="maxBubDist" type="integer" value="50000" label="Maximal probing distance for bubble popping"
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51 help="Bubbles longer than this value will not be popped" />
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52 <param argument="-e" name="minUtgSize" type="integer" value="4" label="A unitig is considered small if it is composed of less than this many reads"
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53 help="Miniasm may try to remove small unitigs at various steps"/>
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54 <param argument="-n" name="nRounds" type="integer" value="3" label="Rounds of short overlap removal" />
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55 <param argument="-F" name="finalDropRatio" type="float" value="0.8" label="Overlap drop ratio threshold after short unitig removal" />
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56 </section>
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57 </inputs>
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58 <outputs>
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59 <data name="gfa" format="tabular" label="${tool.name} on ${on_string}: Assembly Graph" />
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60 </outputs>
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61 <tests>
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62 <test>
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63 <param name="readFile" value="mini_reads.fq"/>
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64 <param name="paf" value="mini_reads.paf.gz"/>
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65 <output name="gfa" file="mini_reads.gfa"/>
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66 </test>
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67 <test>
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68 <param name="readFile" value="mini_reads.fq.gz"/>
0
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69 <param name="minMatch" value="99"/>
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70 <param name="minIden" value="0.04"/>
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71 <param name="minSpan" value="999"/>
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72 <param name="minCov" value="2"/>
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73 <param name="minOvlp" value="999"/>
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74 <param name="maxHang" value="999"/>
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75 <param name="intThres" value="0.7"/>
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76 <param name="maxGapDiff" value="999"/>
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77 <param name="maxBubDist" value="45000"/>
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78 <param name="minUtgSize" value="3"/>
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79 <param name="nRounds" value="2"/>
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80 <param name="finalDropRatio" value="0.7"/>
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81 <param name="paf" value="mini_reads.paf.gz"/>
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82 <output name="gfa" file="reads_advanced.gfa"/>
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83 </test>
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84 </tests>
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85 <help><![CDATA[
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86 Miniasm is a very fast OLC-based de novo assembler for noisy long reads. It takes all-vs-all read self-mappings
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87 (typically by minimap) as input and outputs an assembly graph in the GFA format. Different from mainstream assemblers,
8b6d0d85252b planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/miniasm commit 4e244b45154a712c786f6bca774e0b25676f4642
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88 miniasm does not have a consensus step. It simply concatenates pieces of read sequences to generate the final unitig
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89 sequences. Thus the per-base error rate is similar to the raw input reads.
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8dee0218ac7d "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/miniasm commit d225098ffed70a721aa688a57b7fe42815a9f042"
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90
8dee0218ac7d "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/miniasm commit d225098ffed70a721aa688a57b7fe42815a9f042"
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91 NOTE: This tool may take a long time depending on the size and characteristics of your dataset.
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92 ]]></help>
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93 <citations>
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94 <citation type="doi">10.1093/bioinformatics/btw152</citation>
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95 </citations>
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96 </tool>
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97