Mercurial > repos > iuc > mummer_mummer

annotate mummer.xml @ 2:fc143c06a842 draft

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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mummer4 commit 96608e94742d17cb73d57201099ff5f5abbef276"
author iuc
date Fri, 24 Jan 2020 15:57:29 -0500
parents 48badcd29c5d
children 48823c9635a4
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fc143c06a842 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mummer4 commit 96608e94742d17cb73d57201099ff5f5abbef276"
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1 <tool id="mummer_mummer" name="Mummer" version="@MUMMER_VERSION@@WRAPPER_VERSION@">
0
26f2cc1cc1e9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mummer4 commit 8133565adbfc012fa54b96449c2a18d044049107
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2 <description>Align two or more sequences</description>
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
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6 <expand macro="requirements">
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fc143c06a842 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mummer4 commit 96608e94742d17cb73d57201099ff5f5abbef276"
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7 <expand macro="gnuplot_requirement"/>
0
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8 </expand>
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9 <command detect_errors="exit_code">
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10 <![CDATA[
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11 mummer
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12 $anchoring
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13 -l '$min'
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14 $direction
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15 $force
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16 $chars
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17 $print_length
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18 $substring
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19 $position
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20 #if $options.advanced == 'enable'
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21 -k '$options.suffix'
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22 -threads '$options.threads'
26f2cc1cc1e9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mummer4 commit 8133565adbfc012fa54b96449c2a18d044049107
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23 -qthreads '$options.qthreads'
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24 -suflink '$options.suflink'
0
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25 -child '$options.child'
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26 -skip '$options.skip'
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27 -kmer '$options.kmer'
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28 #end if
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29 '$reference_sequence' '$query_sequence' > '$output'
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30 #if $mumplot.plot == 'yes':
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31 && mummerplot
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32 -b '$mumplot.breaklen'
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33 $mumplot.color
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34 $mumplot.coverage
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35 $mumplot.filter
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36 $mumplot.fat
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37 #if $mumplot.labels.IDs == 'yes':
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38 -IdR '$mumplot.labels.ref_id'
26f2cc1cc1e9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mummer4 commit 8133565adbfc012fa54b96449c2a18d044049107
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39 -IdQ '$mumplot.labels.query_id'
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40 #end if
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41 -s '$mumplot.size'
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42 -terminal png
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43 -title '$mumplot.title'
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44 $mumplot.snp
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45 #if $mumplot.range.custom == 'yes':
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46 -x [$mumplot.range.min_x:$mumplot.range.max_x]
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47 -y [$mumplot.range.min_y:$mumplot.range.max_y]
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48 #end if
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49 '$output'
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fc143c06a842 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mummer4 commit 96608e94742d17cb73d57201099ff5f5abbef276"
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50 @MUMMER_GNUPLOT_MANUAL@
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51 #end if
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52 ]]>
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53 </command>
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54 <inputs>
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55 <param name="reference_sequence" type="data" format="fasta" label="Reference Sequence" help="FastA or multi-FastA" />
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56 <param name="query_sequence" type="data" format="fasta" label="Query Sequence" help="FastA or multi-FastA" />
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57 <param name="anchoring" type="select" label="Anchoring" help="Choose a match anchoring strategy">
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58 <option value="">Maximal matches unique in only reference sequence (-mumreference) (Default)</option>
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59 <option value="-mum">Maximal matches unique in both sequences (-mum)</option>
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60 <option value="-maxmatch">All matches reguardless of uniqueness (-maxmatch)</option>
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61 </param>
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62 <param name="min" type="integer" argument="-l" value="20" label="Minimum Match Length" help="Minimum length of a match. (-l)" />
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63 <param name="direction" type="select" label="Direction" help="Direction of complement sequence to use">
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64 <option value="-b">Compute forward and reverse complement matches (-b)</option>
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65 <option value="-r">Compute only reverse complement matches (-r)</option>
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66 </param>
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67 <param name="force" type="boolean" argument="-F" truevalue="-F" falsevalue="" label="Force 4 column output"
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68 help="force 4 column output format regardless of the number of reference sequence inputs. (-F)" />
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69 <param name="chars" type="boolean" argument="-n" truevalue="-n" falsevalue="" label="Match only the characters a, c, g, or t" help="(-n)" />
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70 <param name="print_length" type="boolean" argument="-L" truevalue="-L" falsevalue="" label="Print length of query sequence in header of matches" help="(-L)" />
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71 <param name="substring" type="boolean" argument="-s" truevalue="-s" falsevalue="" label= "Print first 53 characters of the matching substring" help="(-s)" />
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72 <param name="position" type="boolean" argument="-c" truevalue="-c" falsevalue="" label="Position"
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73 help="Report the query position of a reverse complement match relative to the forward strand of the query sequence. (-c)" />
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74 <conditional name="options">
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75 <param name="advanced" type="select" label="Additional options">
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76 <option value="defaults">Use defaults</option>
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77 <option value="enable">Select additional options</option>
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78 </param>
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79 <when value="enable" >
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80 <param name="suffix" type="integer" argument="-k" value="1" label="Suffix" help="Sampled suffix positions. (-k)" />
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81 <param name="threads" type="integer" argument="-threads" value="3" label="Threads"
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82 help="Number of threads to use for -maxmatch; only valid if above parameter is greater than one. (-threads)" />
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83 <param name="qthreads" type="integer" argument="-qthreads" value="3" label="Qthreads" help="Number of threads to use for queries. (-qthreads)" />
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84 <param name="suflink" type="select" label="Suflink" help="Use suffix links in the index and during search? (-suflink)" >
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85 <option value="0">No</option>
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86 <option value="1">Yes</option>
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87 </param>
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88 <param name="child" type="select" label="Child" help="Use child table in the index and during search? (-child)" >
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89 <option value="0">No</option>
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90 <option value="1">Yes</option>
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91 </param>
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92 <param name="skip" type="integer" argument="-skip" value="10" label="Sparsify"
26f2cc1cc1e9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mummer4 commit 8133565adbfc012fa54b96449c2a18d044049107
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93 help="Sparsify the MEM-finding algorithm even more, performing jumps of skip*k [auto (l-10)/k]. (-skip)" />
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94 <param name="kmer" type="integer" argument="-kmer" value="1" label="kmer Table"
26f2cc1cc1e9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mummer4 commit 8133565adbfc012fa54b96449c2a18d044049107
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95 help="Use kmer table containing sa-intervals (speeds up searching first k characters) in the index and during search. (-kmer)" />
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96 </when>
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97 <when value="defaults" />
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98 </conditional>
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99 <conditional name="mumplot" >
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100 <param name="plot" type="select" label="Do you want to output a 2-D dotplot of the input sequences?" >
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101 <option value="yes">YES</option>
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102 <option value="no">NO</option>
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103 </param>
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104 <when value="yes" >
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105 <expand macro="mumplot_input" />
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106 </when>
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107 <when value="no" />
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108 </conditional>
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109 </inputs>
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110 <outputs>
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111 <data name="output" format="tabular" from_work_dir="mummer.txt" label="${tool.name} on ${on_string}: alignment" />
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112 <data name="png_output" format="png" from_work_dir="out.png" label="${tool.name} on ${on_string}: plot" >
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113 <filter>mumplot['plot'] == 'yes'</filter>
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114 </data>
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115 </outputs>
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116 <tests>
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117 <test>
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118 <param name="reference_sequence" ftype="fasta" value="human_aqp3.fasta" />
26f2cc1cc1e9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mummer4 commit 8133565adbfc012fa54b96449c2a18d044049107
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119 <param name="query_sequence" ftype="fasta" value="mouse_aqp3.fasta" />
26f2cc1cc1e9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mummer4 commit 8133565adbfc012fa54b96449c2a18d044049107
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120 <param name="options" value="defaults" />
26f2cc1cc1e9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mummer4 commit 8133565adbfc012fa54b96449c2a18d044049107
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121 <param name="plot" value="yes" />
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122 <output name="output" ftype="tabular" compare="diff" value="mummer.txt" />
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123 <output name="png_output" ftype="png" compare="sim_size" value="plot.png" />
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124 </test>
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125 </tests>
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126 <help><![CDATA[
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127 This is the core program of the MUMmer package. It is the suffix-tree based match finding routine, and the main part of every MUMmer script. By default, mummer now finds maximal matches regardless of their uniqueness. Limiting the output to only unique matches can be specified by choosing alternative Anchoring strategies.
26f2cc1cc1e9 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mummer4 commit 8133565adbfc012fa54b96449c2a18d044049107
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128
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129 **Options:**::
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130
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131 mummer
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132
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133 -mumreference Compute maximal matches that are unique in the reference- sequence but not
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134 necessarily in the query-sequence (default)
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135
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136 -maxmatch Compute all maximal matches regardless of their uniqueness
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137
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138 -l Set the minimum length of a match
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139
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140 -b Compute forward and reverse complement matches
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141
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142 -r Compute only reverse complement matches
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143
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144 -mum Compute maximal matches that are unique in both sequences
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145
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146 -F Force 4 column output format regardless of the number of reference sequence inputs
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147
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148 -n Match only the characters a, c, g, or t
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149
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150 -L Print length of query sequence in header of matches
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151
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152 -s Print first 53 characters of the matching substring
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153
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154 -c Report the query position of a reverse complement match
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155 relative to the forward strand of the query sequence
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156
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157 mummerplot
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158
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159 -b Highlight alignments with breakpoints further than breaklen nucleotides from the nearest
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160 sequence end
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161
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162 -color Color plot lines with a percent similarity gradient or turn off all plot color (default
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163 color by match dir) If the plot is very sparse, edit the .gp script to plot with
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164 'linespoints' instead of 'lines'
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165
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166 -c Generate a reference coverage plot (default for .tiling)
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167
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168 --filter Only display .delta alignments which represent the "best" hit to any particular spot on
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169 either sequence, i.e. a one-to-one mapping of reference and query subsequences
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170
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171 --fat Layout sequences using fattest alignment only
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172
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173 -IdR Plot a particular reference sequence ID on the X-axis
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174
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175 -IdQ Plot a particular query sequence ID on the Y-axis
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176
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177 -s Set the output size to small, medium or large (--small) (--medium) (--large) (default 'small')
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178
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179 --SNP Highlight SNP locations in each alignment
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180
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181 -title Specify the gnuplot plot title (default none)
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182
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183 -x Set the xrange for the plot '[min:max]'
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184
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185 -y Set the yrange for the plot '[min:max]'
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186
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187 -R Plot an ordered set of reference sequences from Rfile
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188
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189 -Q Plot an ordered set of query sequences from Qfile
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190
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191 --layout Layout a .delta multiplot in an intelligible fashion, this option requires the -R -Q options
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192
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193 ]]></help>
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194 <expand macro="citation" />
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195 </tool>