Mercurial > repos > lparsons > cutadapt
annotate cutadapt.xml @ 18:5c7e7d905b33 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
author | iuc |
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date | Thu, 13 Dec 2018 20:54:04 -0500 |
parents | 52ef1be47553 |
children | 49370cb85f0f |
rev | line source |
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16
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
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1 <tool id="cutadapt" name="Cutadapt" version="1.16.4" profile="17.09"> |
9 | 2 <description>Remove adapter sequences from Fastq/Fasta</description> |
11
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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changeset
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3 <macros> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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changeset
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4 <import>macros.xml</import> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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changeset
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5 </macros> |
9 | 6 <requirements> |
11
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents:
10
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changeset
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7 <requirement type="package" version="1.16">cutadapt</requirement> |
9 | 8 </requirements> |
10
01d94df2e32a
planemo upload for repository https://bitbucket.org/lance_parsons/cutadapt_galaxy_wrapper
lparsons
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9 |
8
2d6671b10919
Updated to support cutadapt version 1.1 (also include automatic dependency installation)
lparsons
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10 <version_command>cutadapt --version</version_command> |
0
8b064ea16722
Initial version with multiple adapter support
Lance Parsons <lparsons@princeton.edu>
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11 |
11
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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12 <command detect_errors="exit_code"><![CDATA[ |
13
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
parents:
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13 ## Link in the input and output files, so Cutadapt can tell their type |
11
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents:
10
diff
changeset
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14 |
13
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
parents:
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changeset
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15 #import re |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
parents:
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changeset
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16 #set read1 = "input_f" |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
parents:
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changeset
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17 #set read2 = "input_r" |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
parents:
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changeset
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18 #set paired = False |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
parents:
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19 #set library_type = str($library.type) |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
parents:
12
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changeset
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20 #if $library_type == 'paired': |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
parents:
12
diff
changeset
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21 #set paired = True |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
parents:
12
diff
changeset
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22 #set read1 = re.sub('[^\w\-\s]', '_', str($library.input_1.element_identifier)) |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
parents:
12
diff
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23 #set read2 = re.sub('[^\w\-\s]', '_', str($library.input_2.element_identifier)) |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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12
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24 #set input_1 = $library.input_1 |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
parents:
12
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changeset
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25 #set input_2 = $library.input_2 |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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12
diff
changeset
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26 #else if $library_type == 'paired_collection' |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
parents:
12
diff
changeset
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27 #set paired = True |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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28 #set input_1 = $library.input_1.forward |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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12
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29 #set input_2 = $library.input_1.reverse |
14
c76ee709ffb9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 32b4794bb410494ffcfa0da397aaca7fd830a705
iuc
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30 #set read1 = re.sub('[^\w\-\s]', '_', str($library.input_1.name)) + "_1" |
c76ee709ffb9
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 32b4794bb410494ffcfa0da397aaca7fd830a705
iuc
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31 #set read2 = re.sub('[^\w\-\s]', '_', str($library.input_1.name)) + "_2" |
13
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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32 #else |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
parents:
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33 #set input_1 = $library.input_1 |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
parents:
12
diff
changeset
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34 #set read1 = re.sub('[^\w\-\s]', '_', str($library.input_1.element_identifier)) |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
parents:
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changeset
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35 #end if |
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8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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36 |
13
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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37 #if $input_1.is_of_type("fastq.gz", "fastqsanger.gz"): |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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12
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38 #set read1 = $read1 + ".fq.gz" |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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12
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changeset
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39 #set out1 = "out1.gz" |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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12
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40 #else if $input_1.is_of_type("fastq.bz2", "fastqsanger.bz2"): |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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12
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41 #set read1 = $read1 + ".fq.bz2" |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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12
diff
changeset
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42 #set out1 = "out1.bz2" |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
parents:
12
diff
changeset
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43 #else if $input_1.is_of_type('fasta'): |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
parents:
12
diff
changeset
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44 #set read1 = $read1 + ".fa" |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
parents:
12
diff
changeset
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45 #set out1 = "out1.fa" |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
parents:
12
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46 #else: |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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12
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47 #set read1 = $read1 + ".fq" |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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12
diff
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48 #set out1 = "out1.fq" |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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49 #end if |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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50 ln -f -s '${input_1}' '$read1' && |
12
78e1cf88d133
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
iuc
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51 |
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f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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52 #if $paired: |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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12
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53 #if $input_2.is_of_type("fastq.gz", "fastqsanger.gz"): |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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54 #set read2 = $read2 + ".fq.gz" |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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12
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55 #set out2 = "out2.gz" |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
parents:
12
diff
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56 #else if $input_2.is_of_type("fastq.bz2", "fastqsanger.bz2"): |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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12
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57 #set read2 = $read2 + ".fq.bz2" |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
parents:
12
diff
changeset
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58 #set out2 = "out2.bz2" |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
parents:
12
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59 #else if $input_2.is_of_type('fasta'): |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
parents:
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60 #set read2 = $read2 + ".fa" |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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12
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61 #set out2 = "out2.fa" |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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62 #else: |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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63 #set read2 = $read2 + ".fq" |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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64 #set out2 = "out2.fq" |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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65 #end if |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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66 ln -f -s '${input_2}' '$read2' && |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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67 #end if |
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8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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68 |
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f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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69 ## Run Cutadapt |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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70 |
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8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
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71 #if $output_options.multiple_output: |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
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72 mkdir split && |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
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73 #end if |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
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74 |
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f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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75 cutadapt |
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8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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76 |
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f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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77 ## cutadapt (up to version 1.16) can't be run in multicore mode with these options |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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78 #if not any(($output_options.info_file, $output_options.rest_file, $output_options.wildcard_file, $output_options.too_short_file, $output_options.too_long_file, $output_options.untrimmed_file)) |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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79 -j \${GALAXY_SLOTS:-1} |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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80 #end if |
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8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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81 |
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f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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82 #if str( $library.type ) == "single": |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
iuc
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83 @read1_options@ |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
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84 #if $output_options.multiple_output: |
18
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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85 --output='split/{name}.${input_1.ext}' |
16
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
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86 #else: |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
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87 --output='$out1' |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
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88 #end if |
13
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
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89 #else: |
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90 @read1_options@ |
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91 @read2_options@ |
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92 --output='$out1' |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
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93 --paired-output='$out2' |
f5fdf41c08b8
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94 #end if |
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Updated to support cutadapt version 1.1 (also include automatic dependency installation)
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95 |
13
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96 --error-rate=$adapter_options.error_rate |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
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97 --times=$adapter_options.count |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
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98 --overlap=$adapter_options.overlap |
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99 $adapter_options.no_indels |
f5fdf41c08b8
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100 $adapter_options.match_read_wildcards |
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101 $adapter_options.no_trim |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
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102 $adapter_options.mask_adapter |
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103 |
13
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104 $filter_options.discard |
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105 $filter_options.discard_untrimmed |
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106 #if str($filter_options.min) != '0': |
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107 --minimum-length=$filter_options.min |
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108 #end if |
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109 #if str($filter_options.max) != '0': |
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110 --maximum-length=$filter_options.max |
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111 #end if |
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112 #if $filter_options.max_n: |
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113 --max-n=$filter_options.max_n |
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114 #end if |
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115 #if str( $library.type ) != "single": |
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116 #if $filter_options.pair_filter: |
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117 --pair-filter=$filter_options.pair_filter |
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118 #end if |
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119 #end if |
9 | 120 |
121 | |
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122 #if str($read_mod_options.quality_cutoff) != '0': |
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123 --quality-cutoff=$read_mod_options.quality_cutoff |
f5fdf41c08b8
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1663496ce146a311569a340b0b809dc5127e0830
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124 #end if |
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125 #if str($read_mod_options.nextseq_trim) != '0': |
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126 --nextseq-trim=$read_mod_options.nextseq_trim |
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127 #end if |
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128 $read_mod_options.trim_n |
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129 #if $read_mod_options.prefix != '': |
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130 --prefix="$read_mod_options.prefix" |
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131 #end if |
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132 #if $read_mod_options.suffix != '': |
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133 --suffix="$read_mod_options.suffix" |
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134 #end if |
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135 #if str($read_mod_options.length) != '0': |
17
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 5b7bf5d9a56f6aabaf7951d3e32a082c91937360
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136 --length=$read_mod_options.length |
13
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137 #end if |
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138 #if $read_mod_options.length_tag != '': |
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139 --length-tag="$read_mod_options.length_tag" |
f5fdf41c08b8
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140 #end if |
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Updated to support cutadapt version 1.1 (also include automatic dependency installation)
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141 |
13
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142 '${read1}' |
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143 #if $paired: |
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144 '${read2}' |
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145 #end if |
0
8b064ea16722
Initial version with multiple adapter support
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146 |
13
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147 #if $output_options.report: |
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148 > report.txt |
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149 #end if |
11
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150 ]]></command> |
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151 <inputs> |
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152 |
11
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153 <!-- Reads --> |
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154 <conditional name="library"> |
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155 <param name="type" type="select" label="Single-end or Paired-end reads?"> |
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156 <option value="single">Single-end</option> |
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157 <option value="paired">Paired-end</option> |
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158 <option value="paired_collection">Paired-end Collection</option> |
8665bcc8b847
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159 </param> |
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8b064ea16722
Initial version with multiple adapter support
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160 |
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161 <when value="single"> |
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162 <param name="input_1" format="fastq.gz,fastq,fastqsanger,fastqsanger.gz,fastqsanger.bz2,fasta" type="data" label="FASTQ/A file" help="Should be of datatype "fastq.gz" or "fasta"" /> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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163 <expand macro="single_end_options" /> |
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164 </when> |
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165 |
11
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166 <when value="paired"> |
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167 <param name="input_1" format="fastqsanger,fastqsanger.gz,fastqsanger.bz2,fasta" type="data" label="FASTQ/A file #1" help="Should be of datatype "fastq.gz"or "fasta"" /> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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168 <param name="input_2" format="fastqsanger,fastqsanger.gz,fastqsanger.bz2,fasta" type="data" label="FASTQ/A file #2" help="Should be of datatype "fastq.gz"or "fasta"" /> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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169 <expand macro="paired_end_options" /> |
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170 </when> |
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171 |
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172 <when value="paired_collection"> |
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173 <param name="input_1" format="fastqsanger,fastqsanger.gz,fastqsanger.bz2,fasta" type="data_collection" collection_type="paired" label="Paired Collection" help="Should be of datatype "fastq.gz" or "fasta"" /> |
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174 <expand macro="paired_end_options" /> |
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175 </when> |
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176 |
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177 </conditional> |
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178 |
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179 <!-- Adapter Options --> |
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180 <section name="adapter_options" title="Adapter Options"> |
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181 <param name="error_rate" argument="--error-rate" type="float" min="0" max="1" value="0.1" label="Maximum error rate" help="Maximum allowed error rate (no. of errors divided by the length of the matching region)." /> |
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182 <param name="no_indels" argument="--no-indels" type="boolean" value="False" truevalue="--no-indels" falsevalue="" label="Do not allow indels (Use ONLY with anchored 5' (front) adapters)." help="Do not allow indels in the alignments. That is, allow only mismatches. This option is currently only supported for anchored 5' adapters ('^ADAPTER') (default: both mismatches and indels are allowed)." /> |
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183 <param name="count" argument="--times" type="integer" min="1" value="1" label="Match times" help="Try to remove adapters at most COUNT times. Useful when an adapter gets appended multiple times." /> |
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184 <param name="overlap" argument="--overlap" type="integer" min="1" value="3" label="Minimum overlap length" help="Minimum overlap length. If the overlap between the adapter and the sequence is shorter than LENGTH, the read is not modified. This reduces the number of bases trimmed purely due to short random adapter matches." /> |
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185 <param name="match_read_wildcards" type="select" label="Match Wildcards" help="Allow 'N's as matches. Default: In the adapters but not in the reads"> |
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186 <option value=" " selected="True">In the adapters but not in the reads</option> |
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187 <option value="--match-read-wildcards">In the adapters and in the reads</option> |
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188 <option value="--no-match-adapter-wildcards">Nowhere</option> |
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189 </param> |
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190 <param name="no_trim" argument="--no-trim" type="boolean" value="False" truevalue="--no-trim" falsevalue="" label="Do not trim adapters" help="Match and redirect reads to output/untrimmed-output as usual, but don't remove the adapters (default: trim the adapters)." /> |
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191 <param name="mask_adapter" argument="--mask-adapter" type="boolean" value="False" truevalue="--mask-adapter" falsevalue="" label="Mask Adapters" help="Mask adapter bases with 'N' instead of trimming them (default: trim adapters)." /> |
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192 </section> |
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193 |
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194 <!-- Filter Options --> |
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195 <section name="filter_options" title="Filter Options"> |
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196 <param name="discard" argument="--discard-trimmed" type="boolean" value="False" truevalue="--discard" falsevalue="" label="Discard Trimmed Reads" help="Discard reads that contain the adapter instead of trimming them. Use the 'Minimum overlap length' option in order to avoid throwing away too many randomly matching reads!" /> |
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197 <param name="discard_untrimmed" argument="--discard_untrimmed" type="boolean" value="False" truevalue="--discard-untrimmed" falsevalue="" label="Discard Untrimmed Reads" help="Discard reads that do not contain the adapter." /> |
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198 <param name="min" argument="--minimum-length" type="integer" min="0" optional="True" value="0" label="Minimum length" help="Discard trimmed reads that are shorter than LENGTH. Reads that are too short even before adapter removal are also discarded. In colorspace, an initial primer is not counted. Value of 0 means no minimum length." /> |
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199 <param name="max" argument="--maximum-length" type="integer" min="0" optional="True" value="0" label="Maximum length" help="Discard trimmed reads that are longer than LENGTH. Reads that are too long even before adapter removal are also discarded. In colorspace, an initial primer is not counted. Value of 0 means no maximum length." /> |
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200 <param name="max_n" argument="--max-n" type="float" min="0" optional="True" label="Max N" help="Discard reads with more than this number of 'N' bases. A number between 0 and 1 is interpreted as a fraction of the read length." /> |
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201 <param name="pair_filter" argument="--pair-filter" type="select" optional="True" label="Pair filter" help="Which of the reads in a paired-end read have to match the filtering criterion in order for the pair to be filtered. Default: any"> |
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202 <option value="any" selected="True">any</option> |
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203 <option value="both">both</option> |
9 | 204 </param> |
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205 </section> |
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206 |
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207 <!-- Read Modification Options --> |
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208 <section name="read_mod_options" title="Read Modification Options"> |
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209 <param name="quality_cutoff" argument="--quality-cutoff" type="text" value="0" label="Quality cutoff" help=" Trim low-quality bases from 5' and/or 3' ends of each read before adapter removal. Applied to both reads if data is paired. If one value is given, only the 3' end is trimmed. If two comma-separated cutoffs are given, the 5' end is trimmed with the first cutoff, the 3' end with the second."> |
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210 <sanitizer> |
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211 <valid initial="string.digits"><add value="," /></valid> |
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212 </sanitizer> |
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213 </param> |
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214 <param name="nextseq_trim" argument="--nextseq-trim" type="integer" value="0" label="NextSeq trimming" help="Experimental option for quality trimming of NextSeq data. This is necessary because that machine cannot distinguish between G and reaching the end of the fragment (it encodes G as ‘black’). This option works like regular quality trimming (where one would use -q 20 instead), except that the qualities of G bases are ignored." /> |
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215 <param name="trim_n" argument="--trim-n" type="boolean" truevalue="--trim-n" falsevalue="" checked="False" label="Trim Ns" help="Trim N's on ends of reads." /> |
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216 <param name="prefix" argument="--prefix" label="Prefix" type="text" help="Add this prefix to read names" /> |
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217 <param name="suffix" argument="--suffix" label="Suffix" type="text" help="Add this suffix to read names" /> |
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218 <param name="strip_suffix" argument="--strip-suffix" label="Strip suffix" type="text" help="Remove this suffix from read names if present." /> |
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219 <param name="length" argument="--length" type="integer" value="0" label="Length" help="Shorten reads to this length. This modification is applied after adapter trimming." /> |
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220 <param name="length_tag" argument="--length-tag" label="Length Tag" type="text" help="Search for TAG followed by a decimal number in the name of the read (description/comment field of the FASTA or FASTQ file). Replace the decimal number with the correct length of the trimmed read. For example, use --length-tag 'length=' to search for fields like 'length=123'." /> |
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221 </section> |
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222 |
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223 <!-- Output Options --> |
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224 <section name="output_options" title="Output Options"> |
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225 <param name="report" type="boolean" value="False" label="Report" help="Cutadapt's per-adapter statistics. You can use this file with MultiQC."/> |
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226 <param name="info_file" argument="--info-file" type="boolean" value="False" label="Info File" help="Write information about each read and its adapter matches to a file."/> |
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227 <param name="rest_file" argument="--rest-file" type="boolean" value="False" label="Rest of Read" help="When the adapter matches in the middle of a read, write the rest (after the adapter) into a file."/> |
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228 <param name="wildcard_file" argument="--wildcard-file" type="boolean" value="False" label="Wildcard File" help="When the adapter has wildcard bases ('N's) write adapter bases matching wildcard positions to file."/> |
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229 <param name="too_short_file" argument="--too-short-output" type="boolean" value="False" label="Too Short Reads" help="Write reads that are too short (according to minimum length specified) to a file. (default: discard reads)"/> |
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230 <param name="too_long_file" argument="--too-long-output" type="boolean" value="False" label="Too Long Reads" help="Write reads that are too long (according to maximum length specified) to a file. (default: discard reads)"/> |
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231 <param name="untrimmed_file" argument="--untrimmed-output" type="boolean" value="False" label="Untrimmed Reads" help="Write reads that do not contain the adapter to a separate file, instead of writing them to the regular output file. (default: output to same file as trimmed)"/> |
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232 <param name="multiple_output" argument="" type="boolean" value="False" label="Multiple output" help="Create a separate file for each adapter trimmed (default: all trimmed reads are in a single file)"/> |
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233 </section> |
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234 |
9 | 235 </inputs> |
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236 |
9 | 237 <outputs> |
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238 <data name="out1" format="fastqsanger" metadata_source="input_1" from_work_dir="out1*" label="${tool.name} on ${on_string}: Read 1 Output"> |
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239 <filter>(output_options['multiple_output'] is False)</filter> |
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240 <expand macro="inherit_format_1" /> |
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241 </data> |
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242 <data name="out2" format="fastqsanger" metadata_source="input_2" from_work_dir="out2*" label="${tool.name} on ${on_string}: Read 2 Output" > |
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243 <filter>(library['type'] == 'paired' or library['type'] == 'paired_collection')</filter> |
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244 <expand macro="inherit_format_2" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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245 </data> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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246 |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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247 <data name="report" format="txt" from_work_dir="report.txt" label="${tool.name} on ${on_string}: Report"> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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248 <filter>(output_options['report'] is True)</filter> |
9 | 249 </data> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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250 |
12
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
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251 <data name="info_file" format="txt" metadata_source="input_1" label="${tool.name} on ${on_string}: Info File" > |
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252 <filter>(output_options['info_file'] is True)</filter> |
9 | 253 </data> |
11
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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254 |
12
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
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255 <data name="rest_output" format="fastqsanger" metadata_source="input_1" label="${tool.name} on ${on_string}: Rest of Reads (R1 only)" > |
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256 <filter>(output_options['rest_file'] is True)</filter> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
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257 <expand macro="inherit_format_1" /> |
9 | 258 </data> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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259 |
12
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
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260 <data name="wild_output" format="txt" metadata_source="input_1" label="${tool.name} on ${on_string}: Wildcard File" > |
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261 <filter>(output_options['wildcard_file'] is True)</filter> |
9 | 262 </data> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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263 |
12
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
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264 <data name="untrimmed_output" format="fastqsanger" metadata_source="input_1" label="${tool.name} on ${on_string}: Untrimmed Read 1" > |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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265 <filter>(output_options['untrimmed_file'] is True)</filter> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
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266 <expand macro="inherit_format_1" /> |
9 | 267 </data> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
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268 <data name="untrimmed_paired_output" format="fastqsanger" metadata_source="input_2" label="${tool.name} on ${on_string}: Untrimmed Read 2" > |
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269 <filter>(library['type'] == 'paired' or library['type'] == 'paired_collection')</filter> |
8665bcc8b847
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270 <filter>(output_options['untrimmed_file'] is True)</filter> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
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271 <expand macro="inherit_format_2" /> |
11
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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272 </data> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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273 |
12
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
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274 <data name="too_short_output" format="fastqsanger" metadata_source="input_1" label="${tool.name} on ${on_string}: Too Short Read 1" > |
11
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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275 <filter>(output_options['too_short_file'] is True)</filter> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
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276 <expand macro="inherit_format_1" /> |
9 | 277 </data> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
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278 <data name="too_short_paired_output" format="fastqsanger" metadata_source="input_2" label="${tool.name} on ${on_string}: Too Short Read 2" > |
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279 <filter>(library['type'] == 'paired' or library['type'] == 'paired_collection')</filter> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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280 <filter>(output_options['too_short_file'] is True)</filter> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
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281 <expand macro="inherit_format_2" /> |
9 | 282 </data> |
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283 |
12
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284 <data name="too_long_output" format="fastqsanger" metadata_source="input_1" label="${tool.name} on ${on_string}: Too Long Read 1" > |
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8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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285 <filter>(output_options['too_long_file'] is True)</filter> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
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286 <expand macro="inherit_format_1" /> |
11
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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287 </data> |
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288 <data name="too_long_paired_output" format="fastqsanger" metadata_source="input_2" label="${tool.name} on ${on_string}: Too Long Read 2" > |
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289 <filter>(library['type'] == 'paired' or library['type'] == 'paired_collection')</filter> |
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290 <filter>(output_options['too_long_file'] is True)</filter> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
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291 <expand macro="inherit_format_2" /> |
9 | 292 </data> |
16
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
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293 |
18
5c7e7d905b33
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294 <collection name="split_output" type="list" label="${tool.name} on ${on_string}: Split outputs" > |
5c7e7d905b33
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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295 <discover_datasets pattern="(?P<designation>.+)\.(?P<ext>fastq.*)" directory="split" /> |
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296 <filter>(output_options['multiple_output'] is True)</filter> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
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297 </collection> |
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298 |
9 | 299 </outputs> |
300 | |
301 <tests> | |
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302 <!-- Ensure fastq works --> |
9 | 303 <test> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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304 <param name="type" value="single" /> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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305 <param name="input_1" ftype="fastq" value="cutadapt_small.fastq" /> |
8665bcc8b847
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306 <param name="adapter_source_list" value="user"/> |
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307 <param name="adapter" value="AGATCGGAAGAGC"/> |
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308 <output name="out1" file="cutadapt_small.out" ftype="fastq"/> |
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309 </test> |
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310 <!-- Ensure single end fastq.gz works --> |
8665bcc8b847
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311 <test> |
8665bcc8b847
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312 <param name="type" value="single" /> |
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313 <param name="input_1" ftype="fastq.gz" value="bwa-mem-fastq1.fq.gz" /> |
8665bcc8b847
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314 <param name="adapter_source_list" value="user"/> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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315 <param name="adapter" value="AGATCGGAAGAGC"/> |
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316 <output name="out1" decompress="True" file="cutadapt_out1.fq.gz" ftype="fastq.gz"/> |
9 | 317 </test> |
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318 <!-- Ensure paired end fastq.gz works --> |
9 | 319 <test> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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320 <param name="type" value="paired" /> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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321 <param name="input_1" ftype="fastq.gz" value="bwa-mem-fastq1.fq.gz" /> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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322 <param name="input_2" ftype="fastq.gz" value="bwa-mem-fastq2.fq.gz" /> |
8665bcc8b847
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323 <param name="adapter_source_list" value="user"/> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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324 <param name="adapter" value="AGATCGGAAGAGC"/> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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325 <param name="adapter_source_list2" value="user"/> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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326 <param name="adapter2" value="AGATCGGAAGAGC"/> |
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327 <output name="out1" decompress="True" file="cutadapt_out1.fq.gz" ftype="fastq.gz"/> |
78e1cf88d133
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328 <output name="out2" decompress="True" file="cutadapt_out2.fq.gz" ftype="fastq.gz"/> |
78e1cf88d133
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
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329 </test> |
78e1cf88d133
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330 <!-- Ensure paired collection works --> |
78e1cf88d133
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
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331 <test> |
78e1cf88d133
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332 <param name="type" value="paired_collection" /> |
78e1cf88d133
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
iuc
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diff
changeset
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333 <param name="input_1"> |
78e1cf88d133
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
iuc
parents:
11
diff
changeset
|
334 <collection type="paired"> |
78e1cf88d133
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
iuc
parents:
11
diff
changeset
|
335 <element name="forward" ftype="fastq.gz" value="bwa-mem-fastq1.fq.gz" /> |
78e1cf88d133
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
iuc
parents:
11
diff
changeset
|
336 <element name="reverse" ftype="fastq.gz" value="bwa-mem-fastq2.fq.gz" /> |
78e1cf88d133
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
iuc
parents:
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diff
changeset
|
337 </collection> |
78e1cf88d133
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
iuc
parents:
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diff
changeset
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338 </param> |
78e1cf88d133
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
iuc
parents:
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diff
changeset
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339 <param name="adapter_source_list" value="user"/> |
78e1cf88d133
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
iuc
parents:
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diff
changeset
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340 <param name="adapter" value="AGATCGGAAGAGC"/> |
78e1cf88d133
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
iuc
parents:
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diff
changeset
|
341 <param name="adapter_source_list2" value="user"/> |
78e1cf88d133
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
iuc
parents:
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diff
changeset
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342 <param name="adapter2" value="AGATCGGAAGAGC"/> |
78e1cf88d133
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
iuc
parents:
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diff
changeset
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343 <output name="out1" decompress="True" file="cutadapt_out1.fq.gz" ftype="fastq.gz"/> |
78e1cf88d133
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
iuc
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diff
changeset
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344 <output name="out2" decompress="True" file="cutadapt_out2.fq.gz" ftype="fastq.gz"/> |
11
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents:
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diff
changeset
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345 </test> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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diff
changeset
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346 <!-- Ensure built-in adapters work --> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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changeset
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347 <test> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents:
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diff
changeset
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348 <param name="type" value="single" /> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents:
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diff
changeset
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349 <param name="input_1" ftype="fastq" value="cutadapt_small.fastq" /> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents:
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diff
changeset
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350 <param name="adapter_source_list" value="builtin"/> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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diff
changeset
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351 <param name="adapter" value="TGTAGGCC"/> |
12
78e1cf88d133
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
iuc
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diff
changeset
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352 <output name="out1" file="cutadapt_builtin.out" ftype="fastq"/> |
11
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents:
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changeset
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353 </test> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents:
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diff
changeset
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354 <!-- Ensure discard file output works --> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents:
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diff
changeset
|
355 <test> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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diff
changeset
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356 <param name="input_1" ftype="fastq" value="cutadapt_small.fastq" /> |
9 | 357 <param name="adapter_source_list" value="user"/> |
358 <param name="adapter" value="TTAGACATATCTCCGTCG"/> | |
359 <param name="output_filtering" value="filter"/> | |
11
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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changeset
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360 <param name="discard" value="True"/> |
9 | 361 <param name="read_modification" value="none"/> |
362 <param name="output_type" value="default"/> | |
12
78e1cf88d133
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
iuc
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diff
changeset
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363 <output name="out1" file="cutadapt_discard.out" ftype="fastq"/> |
9 | 364 </test> |
11
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents:
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diff
changeset
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365 <!-- Ensure rest file output works --> |
9 | 366 <test> |
11
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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diff
changeset
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367 <param name="input_1" ftype="fasta" value="cutadapt_rest.fa" /> |
9 | 368 <param name="adapter_source_list" value="user"/> |
11
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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diff
changeset
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369 <param name="adapter" value="AAAGATG"/> |
9 | 370 <param name="output_filtering" value="default"/> |
371 <param name="read_modification" value="none"/> | |
372 <param name="output_type" value="additional"/> | |
11
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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changeset
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373 <param name="rest_file" value="True"/> |
12
78e1cf88d133
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
iuc
parents:
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diff
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374 <output name="out1" file="cutadapt_rest.out" ftype="fasta"/> |
78e1cf88d133
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
iuc
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diff
changeset
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375 <output name="rest_output" file="cutadapt_rest2.out" ftype="fasta"/> |
9 | 376 </test> |
11
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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diff
changeset
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377 <!-- Ensure nextseq-trim option works --> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents:
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diff
changeset
|
378 <test> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents:
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diff
changeset
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379 <param name="type" value="single" /> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents:
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diff
changeset
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380 <param name="input_1" ftype="fastq.gz" value="bwa-mem-fastq1.fq.gz" /> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents:
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diff
changeset
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381 <param name="adapter_source_list" value="user"/> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents:
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diff
changeset
|
382 <param name="adapter" value="AGATCGGAAGAGC"/> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents:
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diff
changeset
|
383 <param name="read_modification" value="modify"/> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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diff
changeset
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384 <param name="nextseq_trim" value="20" /> |
12
78e1cf88d133
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
iuc
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diff
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385 <output name="out1" decompress="True" file="cutadapt_nextseq_out.fq.gz" ftype="fastq.gz"/> |
11
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents:
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diff
changeset
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386 </test> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents:
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diff
changeset
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387 <!-- Ensure Report and Info file output work --> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents:
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diff
changeset
|
388 <test> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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diff
changeset
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389 <param name="type" value="single" /> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents:
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diff
changeset
|
390 <param name="input_1" ftype="fastq" value="cutadapt_small.fastq" /> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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changeset
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391 <param name="adapter_source_list" value="user"/> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents:
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diff
changeset
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392 <param name="adapter" value="AGATCGGAAGAGC"/> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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changeset
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393 <param name="report" value="True" /> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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changeset
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394 <param name="info_file" value="True" /> |
12
78e1cf88d133
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
iuc
parents:
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diff
changeset
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395 <output name="out1" value="cutadapt_small.out" ftype="fastq"/> |
11
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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changeset
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396 <output name="report"> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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changeset
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397 <assert_contents> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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398 <has_text text="Summary"/> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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changeset
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399 </assert_contents> |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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changeset
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400 </output> |
12
78e1cf88d133
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
iuc
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changeset
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401 <output name="info_file" value="cutadapt_info_out.txt" ftype="txt"/> |
11
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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402 </test> |
16
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
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changeset
|
403 |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
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diff
changeset
|
404 |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
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changeset
|
405 <test> |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
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changeset
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406 <conditional name="library"> |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
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changeset
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407 <param name="type" value="single" /> |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents:
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changeset
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408 <param name="input_1" ftype="fastq" value="cutadapt_in_split.fastq" /> |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
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changeset
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409 <section name="r1" > |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents:
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diff
changeset
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410 <repeat name="front_adapters"> |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents:
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diff
changeset
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411 <conditional name="front_adapter_source"> |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents:
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diff
changeset
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412 <param name="front_adapter_source_list" value="user"/> |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents:
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diff
changeset
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413 <param name="front_adapter_name" value="A1" /> |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents:
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diff
changeset
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414 <param name="front_adapter" value="^GTCGGTAA" /> |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents:
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diff
changeset
|
415 </conditional> |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents:
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diff
changeset
|
416 </repeat> |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents:
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diff
changeset
|
417 <repeat name="front_adapters"> |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents:
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diff
changeset
|
418 <conditional name="front_adapter_source"> |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents:
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diff
changeset
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419 <param name="front_adapter_source_list" value="user"/> |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents:
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diff
changeset
|
420 <param name="front_adapter_name" value="A2" /> |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents:
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diff
changeset
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421 <param name="front_adapter" value="^AGGTCACT" /> |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents:
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diff
changeset
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422 </conditional> |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
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423 </repeat> |
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424 </section> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
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425 </conditional> |
18
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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426 <param name="report" value="False" /> |
16
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427 <param name="info_file" value="False" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
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428 <param name="multiple_output" value="True" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
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429 <output_collection name="split_output" type="list" count="3"> |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
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430 <element name="A1" value="A1.fastq" ftype="fastq"> |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
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431 </element> |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
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432 <element name="A2" value="A2.fastq" ftype="fastq"> |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
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433 </element> |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
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434 <element name="unknown" value="unknown.fastq" ftype="fastq"> |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
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435 </element> |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
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436 </output_collection> |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
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437 </test> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
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438 |
8f1df515eb87
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
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439 |
18
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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440 <test> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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441 <conditional name="library"> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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442 <param name="type" value="single" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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443 <param name="input_1" ftype="fastq.gz" value="cutadapt_in_split.fastq.gz" /> |
5c7e7d905b33
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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444 <section name="r1" > |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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445 <repeat name="front_adapters"> |
5c7e7d905b33
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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446 <conditional name="front_adapter_source"> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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447 <param name="front_adapter_source_list" value="user"/> |
5c7e7d905b33
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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448 <param name="front_adapter_name" value="A1" /> |
5c7e7d905b33
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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449 <param name="front_adapter" value="^GTCGGTAA" /> |
5c7e7d905b33
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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450 </conditional> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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451 </repeat> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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452 <repeat name="front_adapters"> |
5c7e7d905b33
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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453 <conditional name="front_adapter_source"> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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454 <param name="front_adapter_source_list" value="user"/> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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455 <param name="front_adapter_name" value="A2" /> |
5c7e7d905b33
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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456 <param name="front_adapter" value="^AGGTCACT" /> |
5c7e7d905b33
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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457 </conditional> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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458 </repeat> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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459 </section> |
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460 </conditional> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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461 <param name="report" value="False" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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462 <param name="info_file" value="False" /> |
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463 <param name="multiple_output" value="True" /> |
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464 <output_collection name="split_output" type="list" count="3"> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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465 <element name="A1" decompress="True" file="A1.fastq.gz" ftype="fastq.gz"> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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466 </element> |
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467 <element name="A2" decompress="True" file="A2.fastq.gz" ftype="fastq.gz"> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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468 </element> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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469 <element name="unknown" decompress="True" file="unknown.fastq.gz" ftype="fastq.gz"> |
5c7e7d905b33
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470 </element> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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471 </output_collection> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
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472 </test> |
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473 |
9 | 474 </tests> |
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475 |
11
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476 <help><![CDATA[ |
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477 |
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478 .. class:: infomark |
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479 |
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480 **What it does** |
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481 |
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482 ------------------- |
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483 |
8665bcc8b847
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484 **Cutadapt** finds and removes adapter sequences, primers, poly-A tails and other types of unwanted sequence from your high-throughput sequencing reads. |
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485 |
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486 Cleaning your data in this way is often required: Reads from small-RNA sequencing contain the 3’ sequencing adapter because the read is longer than the molecule that is sequenced, such as in microRNA, or CRISPR data, or Poly-A tails that are useful for pulling out RNA from your sample but often you don’t want them to be in your reads. |
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487 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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488 Cutadapt_ helps with these trimming tasks by finding the adapter or primer sequences in an error-tolerant way. It can also modify and filter reads in various ways. Cutadapt searches for the adapter in all reads and removes it when it finds it. Unless you use a filtering option, all reads that were present in the input file will also be present in the output file, some of them trimmed, some of them not. Even reads that were trimmed entirely (because the adapter was found in the very beginning) are output. All of this can be changed with options in the tool form above. |
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489 |
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490 The tool is based on the **Open Source** Cutadapt_ tool. See the complete `Cutadapt documentation`_ for additional details. If you use Cutadapt, please cite *Marcel, 2011* under **Citations** below. |
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491 |
8665bcc8b847
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492 ------------------- |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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493 |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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494 **Inputs** |
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495 |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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496 ------------------- |
0
8b064ea16722
Initial version with multiple adapter support
Lance Parsons <lparsons@princeton.edu>
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497 |
11
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498 Input files for Cutadapt need to be: |
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499 |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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500 - FASTQ.GZ, FASTQ.BZ2, FASTQ or FASTA |
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501 |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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502 To trim an adapter, input the ADAPTER sequence e.g. AACCGGTT (with the characters: **$**, **^**, **...**, if anchored or linked). |
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503 |
11
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504 ============================================= =================== |
8665bcc8b847
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505 **Option** **Sequence** |
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506 --------------------------------------------- ------------------- |
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507 3’ (End) Adapter ADAPTER |
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508 Anchored 3’ Adapter ADAPTER$ |
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509 |
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510 5’ (Front) Adapter ADAPTER |
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511 Anchored 5’ Adapter ^ADAPTER |
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512 |
8665bcc8b847
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513 5’ or 3’ (Both possible) ADAPTER |
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514 |
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515 Linked Adapter - 3' (End) only ADAPTER1...ADAPTER2 |
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516 Non-anchored Linked Adapter - 5' (Front) only ADAPTER1...ADAPTER2 |
8665bcc8b847
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517 ============================================= =================== |
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518 |
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519 Below is an illustration of the allowed adapter locations relative to the read and depending on the adapter type: |
8665bcc8b847
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520 |
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521 .. image:: $PATH_TO_IMAGES/adapters.svg |
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522 |
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523 |
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524 ------------------- |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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525 |
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526 *Example: Illumina TruSeq Adapters* |
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|
527 |
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528 ------------------- |
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529 |
11
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530 If you have reads containing Illumina TruSeq adapters, for example, follow these steps. |
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531 |
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532 |
11
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533 For Single-end reads as well as the first reads of Paired-end data: |
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534 |
11
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535 **Read 1** |
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536 |
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537 In the **3' (End) Adapters** option above, insert A + the “TruSeq Indexed Adapter” prefix that is common to all Indexed Adapter sequences, e.g insert: |
8665bcc8b847
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538 |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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539 AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC |
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540 |
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541 |
11
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542 For the second reads of Paired-end data: |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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543 |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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544 **Read 2** |
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545 |
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546 In the **3' (End) Adapters** option above, insert the reverse complement of the “TruSeq Universal Adapter”: |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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547 |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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548 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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549 |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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550 The adapter sequences can be found in the document `Illumina TruSeq Adapters De-Mystified`_. |
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551 |
11
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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|
552 ----------- |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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|
553 |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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|
554 **Outputs** |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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555 |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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556 ----------- |
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Initial version with multiple adapter support
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parents:
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changeset
|
557 |
11
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558 - Trimmed reads |
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|
559 |
11
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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560 Optionally, under **Output Options** you can choose to output |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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|
561 |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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562 * Report |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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563 * Info file |
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564 |
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|
565 |
11
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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|
566 **Report** |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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|
567 |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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|
568 Cutadapt can output per-adapter statistics if you select to output the report above. |
8665bcc8b847
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iuc
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|
569 |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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|
570 Example: |
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|
571 |
11
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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572 *This is cutadapt 1.16 with Python 3.6.4* |
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Initial version with multiple adapter support
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parents:
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|
573 |
11
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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574 *Command line parameters: -j 1 --format=fastq -a AGATCGGAAGAGC --info-file=/tmp/tmpX0DlY1/files/000/dataset_21.dat --output=out1.fq --error-rate=0.1 --times=1 --overlap=3 input_f.fastq* |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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|
575 *Running on 1 core* |
8665bcc8b847
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576 *Trimming 1 adapter with at most 10.0% errors in single-end mode ...* |
8665bcc8b847
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577 *Finished in 0.00 s (1426 us/read; 0.04 M reads/minute).* |
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Initial version with multiple adapter support
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|
578 |
11
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|
579 *=== Summary ===* |
0
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|
580 |
11
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581 * Total reads processed: 3* |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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582 * Reads with adapters: 0 (0.0%)* |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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583 * Reads written (passing filters): 3 (100.0%)* |
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Initial version with multiple adapter support
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parents:
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changeset
|
584 |
11
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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585 * Total basepairs processed: 102 bp* |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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586 * Total written (filtered): 102 bp (100.0%)* |
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Initial version with multiple adapter support
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|
587 |
11
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changeset
|
588 *=== Adapter 1 ===* |
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|
589 |
11
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|
590 *Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 0 times.* |
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parents:
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|
591 |
8b064ea16722
Initial version with multiple adapter support
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parents:
diff
changeset
|
592 |
11
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents:
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|
593 **Info file** |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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|
594 |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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changeset
|
595 The info file contains information about the found adapters. The output is a tab-separated text file. Each line corresponds to one read of the input file. |
8665bcc8b847
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iuc
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|
596 |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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|
597 Columns contain the following data: |
9 | 598 |
11
8665bcc8b847
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changeset
|
599 * **1st**: Read name |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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|
600 * **2nd**: Number of errors |
8665bcc8b847
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
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|
601 * **3rd**: 0-based start coordinate of the adapter match |
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602 * **4th**: 0-based end coordinate of the adapter match |
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603 * **5th**: Sequence of the read to the left of the adapter match (can be empty) |
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604 * **6th**: Sequence of the read that was matched to the adapter |
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605 * **7th**: Sequence of the read to the right of the adapter match (can be empty) |
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606 * **8th**: Name of the found adapter |
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607 * **9th**: Quality values corresponding to sequence left of the adapter match (can be empty) |
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608 * **10th**: Quality values corresponding to sequence matched to the adapter (can be empty) |
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609 * **11th**: Quality values corresponding to sequence to the right of the adapter (can be empty) |
9 | 610 |
11
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611 The concatenation of columns 5-7 yields the full read sequence. Column 8 identifies the found adapter. Adapters without a name are numbered starting from 1. Fields 9-11 are empty if quality values are not available. Concatenating them yields the full sequence of quality values. |
9 | 612 |
613 If no adapter was found, the format is as follows: | |
614 | |
11
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615 #. Read name |
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616 #. The value -1 |
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617 #. The read sequence |
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618 #. Quality values |
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619 |
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620 When parsing the file, be aware that additional columns may be added in the future. Note also that some fields can be empty, resulting in consecutive tabs within a line. |
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621 |
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622 If the --times option is used and greater than 1, each read can appear more than once in the info file. There will be one line for each found adapter, all with identical read names. Only for the first of those lines will the concatenation of columns 5-7 be identical to the original read sequence (and accordingly for columns 9-11). For subsequent lines, the shown sequence are the ones that were used in subsequent rounds of adapter trimming, that is, they get successively shorter. |
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623 |
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624 -------------------- |
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625 |
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626 **More Information** |
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627 |
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628 -------------------- |
9 | 629 |
11
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630 See the excellent `Cutadapt documentation`_ |
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631 |
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632 .. _Cutadapt: https://cutadapt.readthedocs.io/en/stable/ |
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633 .. _`Cutadapt documentation`: https://cutadapt.readthedocs.io/en/latest/index.html |
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634 .. _`Illumina TruSeq Adapters De-Mystified`: http://tucf-genomics.tufts.edu/documents/protocols/TUCF_Understanding_Illumina_TruSeq_Adapters.pdf |
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635 |
9 | 636 |
11
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637 -------------------- |
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638 |
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639 **Galaxy Wrapper Development** |
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640 |
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641 -------------------- |
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642 |
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643 Author: Lance Parsons <lparsons@princeton.edu> |
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644 |
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645 ]]></help> |
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646 |
10
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647 <citations> |
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648 <citation type="bibtex"> |
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649 @article{marcel_cutadapt_2011, |
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650 title = {Cutadapt removes adapter sequences from high-throughput sequencing reads}, |
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651 volume = {17}, |
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652 copyright = {Authors who publish with this journal agree to the following terms: Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access ).}, |
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653 url = {http://journal.embnet.org/index.php/embnetjournal/article/view/200}, |
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654 abstract = {When small RNA is sequenced on current sequencing machines, the resulting reads are usually longer than the RNA and therefore contain parts of the 3' adapter. That adapter must be found and removed error-tolerantly from each read before read mapping. Previous solutions are either hard to use or do not offer required features, in particular support for color space data. As an easy to use alternative, we developed the command-line tool cutadapt, which supports 454, Illumina and SOLiD (color space) data, offers two adapter trimming algorithms, and has other useful features. |
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655 |
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656 Cutadapt, including its MIT-licensed source code, is available for download at http://code.google.com/p/cutadapt/}, |
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657 number = {1}, |
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658 urldate = {2011-08-02}, |
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659 journal = {EMBnet.journal}, |
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660 author = {Marcel, Martin}, |
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661 year = {2011}, |
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662 note = {When small RNA is sequenced on current sequencing machines, the resulting reads are usually longer than the RNA and therefore contain parts of the 3' adapter. That adapter must be found and removed error-tolerantly from each read before read mapping. Previous solutions are either hard to use or do not offer required features, in particular support for color space data. As an easy to use alternative, we developed the command-line tool cutadapt, which supports 454, Illumina and SOLiD (color space) data, offers two adapter trimming algorithms, and has other useful features. Cutadapt, including its MIT-licensed source code, is available for download at http://code.google.com/p/cutadapt/}, |
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663 keywords = {Adapter removal;, fastq, MicroRNA, Sequencing, Small RNA, software}, |
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664 file = {Cutadapt removes adapter sequences from high-throughput sequencing reads | Martin | EMBnet.journal:/Users/lparsons/Library/Application Support/Firefox/Profiles/thd2t4je.default/zotero/storage/ZXZT4PSE/200.html:text/html} |
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665 } |
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666 </citation> |
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667 </citations> |
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668 |
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669 </tool> |