annotate cutadapt.xml @ 23:c4b82dce8335 draft

"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit d1e907795fa943b505723dd6f7bf0a61723d8818"
author iuc
date Fri, 30 Apr 2021 10:13:37 +0000
parents 093678460093
children 288f97432497
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c4b82dce8335 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit d1e907795fa943b505723dd6f7bf0a61723d8818"
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1 <tool id="cutadapt" name="Cutadapt" version="1.16.9" profile="17.09">
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2 <description>Remove adapter sequences from Fastq/Fasta</description>
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
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6 <requirements>
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7 <requirement type="package" version="1.16">cutadapt</requirement>
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8 </requirements>
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9
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2d6671b10919 Updated to support cutadapt version 1.1 (also include automatic dependency installation)
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10 <version_command>cutadapt --version</version_command>
0
8b064ea16722 Initial version with multiple adapter support
Lance Parsons <lparsons@princeton.edu>
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12 <command detect_errors="exit_code"><![CDATA[
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13 ## Link in the input and output files, so Cutadapt can tell their type
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14
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15 #import re
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16 #set read1 = "input_f"
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17 #set read2 = "input_r"
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18 #set paired = False
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19 #set library_type = str($library.type)
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20 #if $library_type == 'paired':
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21 #set paired = True
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22 #set read1 = re.sub('[^\w\-\s]', '_', str($library.input_1.element_identifier))
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23 #set read2 = re.sub('[^\w\-\s]', '_', str($library.input_2.element_identifier))
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24 #set input_1 = $library.input_1
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25 #set input_2 = $library.input_2
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26 #else if $library_type == 'paired_collection'
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27 #set paired = True
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28 #set input_1 = $library.input_1.forward
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29 #set input_2 = $library.input_1.reverse
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30 #set read1 = re.sub('[^\w\-\s]', '_', str($library.input_1.name)) + "_1"
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31 #set read2 = re.sub('[^\w\-\s]', '_', str($library.input_1.name)) + "_2"
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32 #else
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33 #set input_1 = $library.input_1
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34 #set read1 = re.sub('[^\w\-\s]', '_', str($library.input_1.element_identifier))
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35 #end if
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36
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37 #if $input_1.is_of_type("fastq.gz", "fastqsanger.gz"):
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38 #set ext = ".fq.gz"
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39 #else if $input_1.is_of_type("fastq.bz2", "fastqsanger.bz2"):
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40 #set ext = ".fq.bz2"
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41 #else if $input_1.is_of_type('fasta'):
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42 #set ext = ".fa"
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43 #else:
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44 #set ext = ".fq"
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45 #end if
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46 #set read1 = $read1 + $ext
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47 #set out1 = "out1" + $ext
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48 #set rest_output = "rest_output" + $ext
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49 #set wild_output = "wild_output" + $ext
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50 #set too_short_output = "too_short_output" + $ext
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51 #set too_long_output = "too_long_output" + $ext
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52 #set untrimmed_output = "untrimmed_output" + $ext
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53 ln -f -s '${input_1}' '$read1' &&
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54
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55 #if $paired:
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56 #if $input_2.is_of_type("fastq.gz", "fastqsanger.gz"):
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57 #set ext2 = ".fq.gz"
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58 #else if $input_2.is_of_type("fastq.bz2", "fastqsanger.bz2"):
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59 #set ext2 = ".fq.gz"
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60 #else if $input_2.is_of_type('fasta'):
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61 #set ext2 = ".fa"
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62 #else:
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63 #set ext2 = ".fq"
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64 #end if
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65 #set read2 = $read2 + $ext2
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66 #set out2 = "out2" + $ext2
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67 #set too_short_paired_output = "too_short_paired_output" + $ext2
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68 #set too_long_paired_output = "too_long_paired_output" + $ext2
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69 #set untrimmed_paired_output = "untrimmed_paired_output" + $ext2
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70 ln -f -s '${input_2}' '$read2' &&
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71 #end if
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72
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73 ## Run Cutadapt
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74
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75 #if $output_options.multiple_output:
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76 mkdir split &&
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77 #end if
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78
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79 cutadapt
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80
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81 ## cutadapt (up to version 1.16) can't be run in multicore mode with these options
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82 #if not any(($output_options.info_file, $output_options.rest_file, $output_options.wildcard_file, $output_options.too_short_file, $output_options.too_long_file, $output_options.untrimmed_file))
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83 -j \${GALAXY_SLOTS:-1}
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84 #end if
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85
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86 #if str( $library.type ) == "single":
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87 @read1_options@
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88 #if $output_options.multiple_output:
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89 --output='split/{name}.${input_1.ext}'
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90 #else:
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91 --output='$out1'
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92 #end if
13
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93 #else:
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94 @read1_options@
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95 @read2_options@
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96 #if $library.type == "paired"
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97 --output='$out1'
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98 --paired-output='$out2'
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99 #else
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100 --output='$out_pairs.forward'
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101 --paired-output='$out_pairs.reverse'
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102 #end if
13
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103 #end if
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104
13
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105 --error-rate=$adapter_options.error_rate
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106 --times=$adapter_options.count
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107 --overlap=$adapter_options.overlap
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108 $adapter_options.no_indels
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109 $adapter_options.match_read_wildcards
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110 $adapter_options.no_trim
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111 $adapter_options.mask_adapter
11
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112
13
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113 $filter_options.discard
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114 $filter_options.discard_untrimmed
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115 #if str($filter_options.min):
13
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116 --minimum-length=$filter_options.min
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117 #end if
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118 #if str($filter_options.max):
13
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119 --maximum-length=$filter_options.max
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120 #end if
22
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121 #if str($filter_options.max_n):
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122 --max-n=$filter_options.max_n
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123 #end if
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124 #if str( $library.type ) != "single":
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125 #if $filter_options.pair_filter:
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126 --pair-filter=$filter_options.pair_filter
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127 #end if
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128 #end if
9
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129
93d58ffe39f1 Updated to version 1.6
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130
13
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131 #if str($read_mod_options.quality_cutoff) != '0':
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132 --quality-cutoff=$read_mod_options.quality_cutoff
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133 #end if
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134 #if str($read_mod_options.nextseq_trim) != '0':
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135 --nextseq-trim=$read_mod_options.nextseq_trim
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136 #end if
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137 $read_mod_options.trim_n
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138 #if $read_mod_options.prefix != '':
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139 --prefix='$read_mod_options.prefix'
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140 #end if
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141 #if $read_mod_options.suffix != '':
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142 --suffix='$read_mod_options.suffix'
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143 #end if
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144 #if str($read_mod_options.length) != '0':
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145 --length=$read_mod_options.length
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146 #end if
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147 #if $read_mod_options.length_tag != '':
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148 --length-tag='$read_mod_options.length_tag'
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149 #end if
8
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150
13
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151 '${read1}'
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152 #if $paired:
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153 '${read2}'
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154 #end if
0
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155
13
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156 #if $output_options.report:
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157 > report.txt
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158 #end if
11
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159 ]]></command>
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160 <inputs>
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161
11
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162 <!-- Reads -->
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163 <conditional name="library">
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164 <param name="type" type="select" label="Single-end or Paired-end reads?">
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165 <option value="single">Single-end</option>
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166 <option value="paired">Paired-end</option>
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167 <option value="paired_collection">Paired-end Collection</option>
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168 </param>
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169
11
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170 <when value="single">
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171 <param name="input_1" format="fastq.gz,fastq,fastqsanger,fastqsanger.gz,fastqsanger.bz2,fasta" type="data" label="FASTQ/A file" help="Should be of datatype &quot;fastq.gz&quot; or &quot;fasta&quot;" />
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172 <expand macro="single_end_options" />
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173 </when>
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174
11
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175 <when value="paired">
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176 <param name="input_1" format="fastqsanger,fastqsanger.gz,fastqsanger.bz2,fasta" type="data" label="FASTQ/A file #1" help="Should be of datatype &quot;fastq.gz&quot;or &quot;fasta&quot;" />
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177 <param name="input_2" format="fastqsanger,fastqsanger.gz,fastqsanger.bz2,fasta" type="data" label="FASTQ/A file #2" help="Should be of datatype &quot;fastq.gz&quot;or &quot;fasta&quot;" />
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178 <expand macro="paired_end_options" />
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179 </when>
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180
11
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181 <when value="paired_collection">
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182 <param name="input_1" format="fastqsanger,fastqsanger.gz,fastqsanger.bz2,fasta" type="data_collection" collection_type="paired" label="Paired Collection" help="Should be of datatype &quot;fastq.gz&quot; or &quot;fasta&quot;" />
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183 <expand macro="paired_end_options" />
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184 </when>
12
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185
8
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186 </conditional>
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187
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188 <!-- Adapter Options -->
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189 <section name="adapter_options" title="Adapter Options">
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190 <param name="error_rate" argument="--error-rate" type="float" min="0" max="1" value="0.1" label="Maximum error rate" help="Maximum allowed error rate (no. of errors divided by the length of the matching region)." />
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191 <param name="no_indels" argument="--no-indels" type="boolean" value="False" truevalue="--no-indels" falsevalue="" label="Do not allow indels (Use ONLY with anchored 5' (front) adapters)." help="Do not allow indels in the alignments. That is, allow only mismatches. This option is currently only supported for anchored 5' adapters ('^ADAPTER') (default: both mismatches and indels are allowed)." />
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192 <param name="count" argument="--times" type="integer" min="1" value="1" label="Match times" help="Try to remove adapters at most COUNT times. Useful when an adapter gets appended multiple times." />
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193 <param name="overlap" argument="--overlap" type="integer" min="1" value="3" label="Minimum overlap length" help="Minimum overlap length. If the overlap between the adapter and the sequence is shorter than LENGTH, the read is not modified. This reduces the number of bases trimmed purely due to short random adapter matches." />
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194 <param name="match_read_wildcards" type="select" label="Match Wildcards" help="Allow 'N's as matches. Default: In the adapters but not in the reads">
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195 <option value=" " selected="True">In the adapters but not in the reads</option>
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196 <option value="--match-read-wildcards">In the adapters and in the reads</option>
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197 <option value="--no-match-adapter-wildcards">Nowhere</option>
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198 </param>
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199 <param name="no_trim" argument="--no-trim" type="boolean" value="False" truevalue="--no-trim" falsevalue="" label="Do not trim adapters" help="Match and redirect reads to output/untrimmed-output as usual, but don't remove the adapters (default: trim the adapters)." />
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200 <param name="mask_adapter" argument="--mask-adapter" type="boolean" value="False" truevalue="--mask-adapter" falsevalue="" label="Mask Adapters" help="Mask adapter bases with 'N' instead of trimming them (default: trim adapters)." />
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201 </section>
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202
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203 <!-- Filter Options -->
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204 <section name="filter_options" title="Filter Options">
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205 <param name="discard" argument="--discard-trimmed" type="boolean" value="False" truevalue="--discard-trimmed" falsevalue="" label="Discard Trimmed Reads" help="Discard reads that contain the adapter instead of trimming them. Use the 'Minimum overlap length' option in order to avoid throwing away too many randomly matching reads!" />
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206 <param name="discard_untrimmed" argument="--discard_untrimmed" type="boolean" value="False" truevalue="--discard-untrimmed" falsevalue="" label="Discard Untrimmed Reads" help="Discard reads that do not contain the adapter." />
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207 <param name="min" argument="--minimum-length" type="integer" min="0" optional="True" value="" label="Minimum length" help="Discard trimmed reads that are shorter than LENGTH. Reads that are too short even before adapter removal are also discarded. In colorspace, an initial primer is not counted." />
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208 <param name="max" argument="--maximum-length" type="integer" min="0" optional="True" value="" label="Maximum length" help="Discard trimmed reads that are longer than LENGTH. Reads that are too long even before adapter removal are also discarded. In colorspace, an initial primer is not counted." />
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209 <param name="max_n" argument="--max-n" type="float" min="0" optional="True" label="Max N" help="Discard reads with more than this number of 'N' bases. A number between 0 and 1 is interpreted as a fraction of the read length." />
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210 <param name="pair_filter" argument="--pair-filter" type="select" optional="True" label="Pair filter" help="Which of the reads in a paired-end read have to match the filtering criterion in order for the pair to be filtered. Default: any">
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211 <option value="any" selected="True">any</option>
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212 <option value="both">both</option>
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213 </param>
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214 </section>
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215
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216 <!-- Read Modification Options -->
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217 <section name="read_mod_options" title="Read Modification Options">
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218 <param name="quality_cutoff" argument="--quality-cutoff" type="text" value="0" label="Quality cutoff" help=" Trim low-quality bases from 5' and/or 3' ends of each read before adapter removal. Applied to both reads if data is paired. If one value is given, only the 3' end is trimmed. If two comma-separated cutoffs are given, the 5' end is trimmed with the first cutoff, the 3' end with the second.">
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219 <sanitizer>
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220 <valid initial="string.digits"><add value="," /></valid>
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221 </sanitizer>
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222 </param>
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223 <param name="nextseq_trim" argument="--nextseq-trim" type="integer" value="0" label="NextSeq trimming" help="Experimental option for quality trimming of NextSeq data. This is necessary because that machine cannot distinguish between G and reaching the end of the fragment (it encodes G as ‘black’). This option works like regular quality trimming (where one would use -q 20 instead), except that the qualities of G bases are ignored." />
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224 <param name="trim_n" argument="--trim-n" type="boolean" truevalue="--trim-n" falsevalue="" checked="False" label="Trim Ns" help="Trim N's on ends of reads." />
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225 <param name="prefix" argument="--prefix" label="Prefix" type="text" help="Add this prefix to read names" />
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226 <param name="suffix" argument="--suffix" label="Suffix" type="text" help="Add this suffix to read names" />
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227 <param name="strip_suffix" argument="--strip-suffix" label="Strip suffix" type="text" help="Remove this suffix from read names if present." />
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228 <param name="length" argument="--length" type="integer" value="0" label="Length" help="Shorten reads to this length. This modification is applied after adapter trimming." />
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229 <param name="length_tag" argument="--length-tag" label="Length Tag" type="text" help="Search for TAG followed by a decimal number in the name of the read (description/comment field of the FASTA or FASTQ file). Replace the decimal number with the correct length of the trimmed read. For example, use --length-tag 'length=' to search for fields like 'length=123'." />
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230 </section>
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231
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232 <!-- Output Options -->
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233 <section name="output_options" title="Output Options">
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234 <param name="report" type="boolean" value="False" label="Report" help="Cutadapt's per-adapter statistics. You can use this file with MultiQC."/>
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235 <param name="info_file" argument="--info-file" type="boolean" value="False" label="Info File" help="Write information about each read and its adapter matches to a file."/>
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236 <param name="rest_file" argument="--rest-file" type="boolean" value="False" label="Rest of Read" help="When the adapter matches in the middle of a read, write the rest (after the adapter) into a file."/>
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237 <param name="wildcard_file" argument="--wildcard-file" type="boolean" value="False" label="Wildcard File" help="When the adapter has wildcard bases ('N's) write adapter bases matching wildcard positions to file."/>
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238 <param name="too_short_file" argument="--too-short-output" type="boolean" value="False" label="Too Short Reads" help="Write reads that are too short (according to minimum length specified) to a file. (default: discard reads)"/>
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239 <param name="too_long_file" argument="--too-long-output" type="boolean" value="False" label="Too Long Reads" help="Write reads that are too long (according to maximum length specified) to a file. (default: discard reads)"/>
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240 <param name="untrimmed_file" argument="--untrimmed-output" type="boolean" value="False" label="Untrimmed Reads" help="Write reads that do not contain the adapter to a separate file, instead of writing them to the regular output file. (default: output to same file as trimmed)"/>
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241 <param name="multiple_output" argument="" type="boolean" value="False" label="Multiple output" help="Create a separate file for each adapter trimmed (default: all trimmed reads are in a single file)"/>
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242 </section>
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243
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244 </inputs>
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245
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246 <outputs>
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247 <data name="out1" format="fastqsanger" metadata_source="input_1" from_work_dir="out1*" label="${tool.name} on ${on_string}: Read 1 Output">
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248 <filter>(output_options['multiple_output'] is False and library['type'] != 'paired_collection')</filter>
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249 <expand macro="inherit_format_1" />
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250 </data>
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251 <data name="out2" format="fastqsanger" metadata_source="input_2" from_work_dir="out2*" label="${tool.name} on ${on_string}: Read 2 Output" >
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252 <filter>(output_options['multiple_output'] is False and library['type'] == 'paired')</filter>
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253 <expand macro="inherit_format_2" />
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254 </data>
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255
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256 <collection name="out_pairs" type="paired" format_source="input_1" label="${tool.name} on ${on_string}: Reads">
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257 <filter>(output_options['multiple_output'] is False and library['type'] == 'paired_collection')</filter>
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258 </collection>
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259
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260 <data name="report" format="txt" from_work_dir="report.txt" label="${tool.name} on ${on_string}: Report">
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261 <filter>(output_options['report'] is True)</filter>
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262 </data>
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263
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264 <data name="info_file" format="txt" metadata_source="input_1" label="${tool.name} on ${on_string}: Info File" >
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265 <filter>(output_options['info_file'] is True)</filter>
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266 </data>
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267
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268 <data name="rest_output" format="fastqsanger" metadata_source="input_1" from_work_dir="rest_output*" label="${tool.name} on ${on_string}: Rest of Reads (R1 only)" >
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269 <filter>(output_options['rest_file'] is True)</filter>
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270 <expand macro="inherit_format_1" />
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271 </data>
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272
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273 <data name="wild_output" format="txt" metadata_source="input_1" from_work_dir="wild_output*" label="${tool.name} on ${on_string}: Wildcard File" >
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274 <filter>(output_options['wildcard_file'] is True)</filter>
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275 </data>
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276
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277 <data name="untrimmed_output" format="fastqsanger" metadata_source="input_1" from_work_dir="untrimmed_output*" label="${tool.name} on ${on_string}: Untrimmed Read 1" >
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278 <filter>(output_options['untrimmed_file'] is True)</filter>
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279 <expand macro="inherit_format_1" />
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280 </data>
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281 <data name="untrimmed_paired_output" format="fastqsanger" metadata_source="input_2" from_work_dir="untrimmed_paired_output*" label="${tool.name} on ${on_string}: Untrimmed Read 2" >
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282 <filter>(library['type'] == 'paired' or library['type'] == 'paired_collection')</filter>
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283 <filter>(output_options['untrimmed_file'] is True)</filter>
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284 <expand macro="inherit_format_2" />
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285 </data>
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286
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287 <data name="too_short_output" format="fastqsanger" metadata_source="input_1" from_work_dir="too_short_output*" label="${tool.name} on ${on_string}: Too Short Read 1" >
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288 <filter>(output_options['too_short_file'] is True)</filter>
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289 <expand macro="inherit_format_1" />
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290 </data>
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291 <data name="too_short_paired_output" format="fastqsanger" metadata_source="input_2" from_work_dir="too_short_paired_output*" label="${tool.name} on ${on_string}: Too Short Read 2" >
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292 <filter>(library['type'] == 'paired' or library['type'] == 'paired_collection')</filter>
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293 <filter>(output_options['too_short_file'] is True)</filter>
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294 <expand macro="inherit_format_2" />
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295 </data>
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296
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297 <data name="too_long_output" format="fastqsanger" metadata_source="input_1" from_work_dir="too_long_output*" label="${tool.name} on ${on_string}: Too Long Read 1" >
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298 <filter>(output_options['too_long_file'] is True)</filter>
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299 <expand macro="inherit_format_1" />
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300 </data>
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301 <data name="too_long_paired_output" format="fastqsanger" metadata_source="input_2" from_work_dir="too_long_paired_output*" label="${tool.name} on ${on_string}: Too Long Read 2" >
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302 <filter>(library['type'] == 'paired' or library['type'] == 'paired_collection')</filter>
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303 <filter>(output_options['too_long_file'] is True)</filter>
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304 <expand macro="inherit_format_2" />
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305 </data>
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306
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307 <collection name="split_output" type="list" label="${tool.name} on ${on_string}: Split outputs" >
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308 <discover_datasets pattern="(?P&lt;designation&gt;.+)\.(?P&lt;ext&gt;fastq.*)" directory="split" />
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309 <filter>(output_options['multiple_output'] is True)</filter>
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310 </collection>
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311
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312 </outputs>
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313
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314 <tests>
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315 <!-- Ensure fastq works -->
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316 <test expect_num_outputs="1">
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317 <param name="type" value="single" />
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318 <param name="input_1" ftype="fastq" value="cutadapt_small.fastq" />
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319 <param name="adapter_source_list" value="user"/>
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320 <param name="adapter" value="AGATCGGAAGAGC"/>
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321 <output name="out1" file="cutadapt_small.out" ftype="fastq"/>
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322 </test>
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323 <!-- Ensure single end fastq.gz works -->
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324 <test expect_num_outputs="1">
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325 <param name="type" value="single" />
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326 <param name="input_1" ftype="fastq.gz" value="bwa-mem-fastq1.fq.gz" />
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327 <param name="adapter_source_list" value="user"/>
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328 <param name="adapter" value="AGATCGGAAGAGC"/>
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329 <output name="out1" decompress="True" file="cutadapt_out1.fq.gz" ftype="fastq.gz"/>
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330 </test>
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331 <!-- Ensure paired end fastq.gz works -->
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332 <test expect_num_outputs="2">
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333 <param name="type" value="paired" />
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334 <param name="input_1" ftype="fastq.gz" value="bwa-mem-fastq1.fq.gz" />
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335 <param name="input_2" ftype="fastq.gz" value="bwa-mem-fastq2.fq.gz" />
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336 <param name="adapter_source_list" value="user"/>
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337 <param name="adapter" value="AGATCGGAAGAGC"/>
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338 <param name="adapter_source_list2" value="user"/>
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339 <param name="adapter2" value="AGATCGGAAGAGC"/>
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340 <output name="out1" decompress="True" file="cutadapt_out1.fq.gz" ftype="fastq.gz"/>
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341 <output name="out2" decompress="True" file="cutadapt_out2.fq.gz" ftype="fastq.gz"/>
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342 <assert_command>
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343 <not_has_text text="--discard-trimmed"/>
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344 <not_has_text text="--discard-untrimmed"/>
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345 <not_has_text text="--minimum-length"/>
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346 <not_has_text text="--maximum-length"/>
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347 <not_has_text text="--max-n"/>
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348 <has_text text="--pair-filter=any"/>
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349 </assert_command>
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350 </test>
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351 <!-- Ensure paired collection works -->
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352 <test expect_num_outputs="3">
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353 <param name="type" value="paired_collection" />
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354 <param name="input_1">
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355 <collection type="paired">
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356 <element name="forward" ftype="fastq.gz" value="bwa-mem-fastq1.fq.gz" />
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357 <element name="reverse" ftype="fastq.gz" value="bwa-mem-fastq2.fq.gz" />
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358 </collection>
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359 </param>
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360 <param name="adapter_source_list" value="user"/>
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361 <param name="adapter" value="AGATCGGAAGAGC"/>
78e1cf88d133 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
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362 <param name="adapter_source_list2" value="user"/>
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363 <param name="adapter2" value="AGATCGGAAGAGC"/>
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364 <output_collection name="out_pairs" type="paired">
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365 <element name="forward" decompress="True" file="cutadapt_out1.fq.gz" ftype="fastq.gz" />
c4b82dce8335 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit d1e907795fa943b505723dd6f7bf0a61723d8818"
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366 <element name="reverse" decompress="True" file="cutadapt_out2.fq.gz" ftype="fastq.gz" />
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367 </output_collection>
11
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368 </test>
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369 <!-- Ensure built-in adapters work -->
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370 <test expect_num_outputs="1">
11
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371 <param name="type" value="single" />
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372 <param name="input_1" ftype="fastq" value="cutadapt_small.fastq" />
8665bcc8b847 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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373 <param name="adapter_source_list" value="builtin"/>
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374 <param name="adapter" value="TGTAGGCC"/>
12
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375 <output name="out1" file="cutadapt_builtin.out" ftype="fastq"/>
11
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376 </test>
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377 <!-- Ensure discard file output works -->
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378 <test expect_num_outputs="1">
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379 <param name="input_1" ftype="fastq" value="cutadapt_small.fastq" />
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380 <param name="adapter_source_list" value="user"/>
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381 <param name="adapter" value="TTAGACATATCTCCGTCG"/>
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diff changeset
382 <param name="output_filtering" value="filter"/>
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383 <section name="filter_options">
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384 <param name="discard" value="True"/>
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
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385 </section>
9
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386 <param name="read_modification" value="none"/>
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387 <param name="output_type" value="default"/>
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388 <output name="out1" file="cutadapt_discard.out" ftype="fastq"/>
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389 <assert_command>
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390 <has_text text="--discard-trimmed"/>
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
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391 </assert_command>
9
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392 </test>
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393 <!-- Ensure rest file output works -->
23
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diff changeset
394 <test expect_num_outputs="2">
11
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395 <param name="input_1" ftype="fasta" value="cutadapt_rest.fa" />
9
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diff changeset
396 <param name="adapter_source_list" value="user"/>
11
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diff changeset
397 <param name="adapter" value="AAAGATG"/>
9
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diff changeset
398 <param name="output_filtering" value="default"/>
93d58ffe39f1 Updated to version 1.6
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diff changeset
399 <param name="read_modification" value="none"/>
93d58ffe39f1 Updated to version 1.6
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parents: 8
diff changeset
400 <param name="output_type" value="additional"/>
11
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parents: 10
diff changeset
401 <param name="rest_file" value="True"/>
12
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parents: 11
diff changeset
402 <output name="out1" file="cutadapt_rest.out" ftype="fasta"/>
78e1cf88d133 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 195c6112eff237b20a6744e82e2c4e7641f7c488
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parents: 11
diff changeset
403 <output name="rest_output" file="cutadapt_rest2.out" ftype="fasta"/>
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parents: 8
diff changeset
404 </test>
11
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parents: 10
diff changeset
405 <!-- Ensure nextseq-trim option works -->
23
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iuc
parents: 22
diff changeset
406 <test expect_num_outputs="1">
11
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parents: 10
diff changeset
407 <param name="type" value="single" />
8665bcc8b847 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents: 10
diff changeset
408 <param name="input_1" ftype="fastq.gz" value="bwa-mem-fastq1.fq.gz" />
8665bcc8b847 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents: 10
diff changeset
409 <param name="adapter_source_list" value="user"/>
8665bcc8b847 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents: 10
diff changeset
410 <param name="adapter" value="AGATCGGAAGAGC"/>
8665bcc8b847 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents: 10
diff changeset
411 <param name="read_modification" value="modify"/>
8665bcc8b847 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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parents: 10
diff changeset
412 <param name="nextseq_trim" value="20" />
12
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diff changeset
413 <output name="out1" decompress="True" file="cutadapt_nextseq_out.fq.gz" ftype="fastq.gz"/>
11
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diff changeset
414 </test>
8665bcc8b847 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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parents: 10
diff changeset
415 <!-- Ensure Report and Info file output work -->
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iuc
parents: 22
diff changeset
416 <test expect_num_outputs="3">
11
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parents: 10
diff changeset
417 <param name="type" value="single" />
8665bcc8b847 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents: 10
diff changeset
418 <param name="input_1" ftype="fastq" value="cutadapt_small.fastq" />
8665bcc8b847 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents: 10
diff changeset
419 <param name="adapter_source_list" value="user"/>
8665bcc8b847 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents: 10
diff changeset
420 <param name="adapter" value="AGATCGGAAGAGC"/>
8665bcc8b847 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
iuc
parents: 10
diff changeset
421 <param name="report" value="True" />
8665bcc8b847 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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parents: 10
diff changeset
422 <param name="info_file" value="True" />
12
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diff changeset
423 <output name="out1" value="cutadapt_small.out" ftype="fastq"/>
11
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diff changeset
424 <output name="report">
8665bcc8b847 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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diff changeset
425 <assert_contents>
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iuc
parents: 10
diff changeset
426 <has_text text="Summary"/>
8665bcc8b847 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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parents: 10
diff changeset
427 </assert_contents>
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diff changeset
428 </output>
12
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diff changeset
429 <output name="info_file" value="cutadapt_info_out.txt" ftype="txt"/>
11
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diff changeset
430 </test>
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parents: 15
diff changeset
431
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diff changeset
432
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parents: 22
diff changeset
433 <test expect_num_outputs="1">
16
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parents: 15
diff changeset
434 <conditional name="library">
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
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parents: 15
diff changeset
435 <param name="type" value="single" />
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents: 15
diff changeset
436 <param name="input_1" ftype="fastq" value="cutadapt_in_split.fastq" />
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents: 15
diff changeset
437 <section name="r1" >
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents: 15
diff changeset
438 <repeat name="front_adapters">
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents: 15
diff changeset
439 <conditional name="front_adapter_source">
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents: 15
diff changeset
440 <param name="front_adapter_source_list" value="user"/>
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents: 15
diff changeset
441 <param name="front_adapter_name" value="A1" />
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
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diff changeset
442 <param name="front_adapter" value="^GTCGGTAA" />
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
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diff changeset
443 </conditional>
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parents: 15
diff changeset
444 </repeat>
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents: 15
diff changeset
445 <repeat name="front_adapters">
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents: 15
diff changeset
446 <conditional name="front_adapter_source">
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents: 15
diff changeset
447 <param name="front_adapter_source_list" value="user"/>
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents: 15
diff changeset
448 <param name="front_adapter_name" value="A2" />
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents: 15
diff changeset
449 <param name="front_adapter" value="^AGGTCACT" />
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents: 15
diff changeset
450 </conditional>
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents: 15
diff changeset
451 </repeat>
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents: 15
diff changeset
452 </section>
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents: 15
diff changeset
453 </conditional>
18
5c7e7d905b33 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
iuc
parents: 17
diff changeset
454 <param name="report" value="False" />
16
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents: 15
diff changeset
455 <param name="info_file" value="False" />
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents: 15
diff changeset
456 <param name="multiple_output" value="True" />
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents: 15
diff changeset
457 <output_collection name="split_output" type="list" count="3">
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents: 15
diff changeset
458 <element name="A1" value="A1.fastq" ftype="fastq">
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents: 15
diff changeset
459 </element>
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents: 15
diff changeset
460 <element name="A2" value="A2.fastq" ftype="fastq">
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents: 15
diff changeset
461 </element>
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents: 15
diff changeset
462 <element name="unknown" value="unknown.fastq" ftype="fastq">
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents: 15
diff changeset
463 </element>
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents: 15
diff changeset
464 </output_collection>
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents: 15
diff changeset
465 </test>
8f1df515eb87 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 034c93600ce3279644d05f3675b4820ef7180851
iuc
parents: 15
diff changeset
466
23
c4b82dce8335 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit d1e907795fa943b505723dd6f7bf0a61723d8818"
iuc
parents: 22
diff changeset
467 <test expect_num_outputs="1">
21
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
468 <conditional name="library">
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
469 <param name="type" value="single" />
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
470 <param name="input_1" ftype="fastq.gz" value="cutadapt_in_split.fastq.gz" />
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
471 <section name="r1" >
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
472 <repeat name="front_adapters">
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
473 <conditional name="front_adapter_source">
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
474 <param name="front_adapter_source_list" value="file"/>
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
475 <param name="front_adapter_file" ftype="fasta" value="barcodes.fasta" />
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
476 </conditional>
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
477 </repeat>
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
478 </section>
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
479 </conditional>
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
480 <param name="report" value="False" />
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
481 <param name="info_file" value="False" />
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
482 <param name="multiple_output" value="True" />
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
483 <output_collection name="split_output" type="list" count="3">
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
484 <element name="A1" decompress="True" file="A1.fastq.gz" ftype="fastq.gz">
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
485 </element>
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
486 <element name="A2" decompress="True" file="A2.fastq.gz" ftype="fastq.gz">
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
487 </element>
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
488 <element name="unknown" decompress="True" file="unknown.fastq.gz" ftype="fastq.gz">
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
489 </element>
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
490 </output_collection>
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
491 </test>
18
5c7e7d905b33 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit b58d6ff959a1d920fd0d9b02e43d2482667d61e8
iuc
parents: 17
diff changeset
492
20
e4691e1589d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 59d42345d86e33e01aa50013f00f4dc0612d3de8
iuc
parents: 19
diff changeset
493 <!-- Ensure untrimmed file output works -->
23
c4b82dce8335 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit d1e907795fa943b505723dd6f7bf0a61723d8818"
iuc
parents: 22
diff changeset
494 <test expect_num_outputs="2">
20
e4691e1589d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 59d42345d86e33e01aa50013f00f4dc0612d3de8
iuc
parents: 19
diff changeset
495 <param name="type" value="single" />
e4691e1589d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 59d42345d86e33e01aa50013f00f4dc0612d3de8
iuc
parents: 19
diff changeset
496 <param name="input_1" ftype="fastq" value="cutadapt_small.fastq" />
e4691e1589d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 59d42345d86e33e01aa50013f00f4dc0612d3de8
iuc
parents: 19
diff changeset
497 <param name="adapter_source_list" value="user"/>
e4691e1589d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 59d42345d86e33e01aa50013f00f4dc0612d3de8
iuc
parents: 19
diff changeset
498 <param name="adapter" value="AAAT"/>
e4691e1589d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 59d42345d86e33e01aa50013f00f4dc0612d3de8
iuc
parents: 19
diff changeset
499 <param name="untrimmed_file" value="True" />
e4691e1589d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 59d42345d86e33e01aa50013f00f4dc0612d3de8
iuc
parents: 19
diff changeset
500 <output name="out1" file="cutadapt_trimmed.out" ftype="fastq"/>
e4691e1589d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 59d42345d86e33e01aa50013f00f4dc0612d3de8
iuc
parents: 19
diff changeset
501 <output name="untrimmed_output" file="cutadapt_untrimmed.out" ftype="fastq"/>
e4691e1589d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 59d42345d86e33e01aa50013f00f4dc0612d3de8
iuc
parents: 19
diff changeset
502 </test>
e4691e1589d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 59d42345d86e33e01aa50013f00f4dc0612d3de8
iuc
parents: 19
diff changeset
503 <!-- Ensure untrimmed gzip file output works -->
23
c4b82dce8335 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit d1e907795fa943b505723dd6f7bf0a61723d8818"
iuc
parents: 22
diff changeset
504 <test expect_num_outputs="2">
20
e4691e1589d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 59d42345d86e33e01aa50013f00f4dc0612d3de8
iuc
parents: 19
diff changeset
505 <param name="type" value="single" />
e4691e1589d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 59d42345d86e33e01aa50013f00f4dc0612d3de8
iuc
parents: 19
diff changeset
506 <param name="input_1" ftype="fastq.gz" value="bwa-mem-fastq1.fq.gz" />
e4691e1589d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 59d42345d86e33e01aa50013f00f4dc0612d3de8
iuc
parents: 19
diff changeset
507 <param name="adapter_source_list" value="user"/>
e4691e1589d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 59d42345d86e33e01aa50013f00f4dc0612d3de8
iuc
parents: 19
diff changeset
508 <param name="adapter" value="AGATCGGAAGAGC"/>
e4691e1589d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 59d42345d86e33e01aa50013f00f4dc0612d3de8
iuc
parents: 19
diff changeset
509 <param name="untrimmed_file" value="True" />
e4691e1589d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 59d42345d86e33e01aa50013f00f4dc0612d3de8
iuc
parents: 19
diff changeset
510 <output name="out1" decompress="True" file="cutadapt_trimmed.out.gz" ftype="fastq.gz"/>
e4691e1589d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 59d42345d86e33e01aa50013f00f4dc0612d3de8
iuc
parents: 19
diff changeset
511 <!--
e4691e1589d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 59d42345d86e33e01aa50013f00f4dc0612d3de8
iuc
parents: 19
diff changeset
512 Do not use the decompress option for this assertion, since it does NOT test that the file is compressed
e4691e1589d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 59d42345d86e33e01aa50013f00f4dc0612d3de8
iuc
parents: 19
diff changeset
513 See discussion at https://github.com/galaxyproject/galaxy/issues/7671
e4691e1589d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 59d42345d86e33e01aa50013f00f4dc0612d3de8
iuc
parents: 19
diff changeset
514 `delta="4000" is more than the difference between gzip level 1 and gzip level 9, but much less than the
e4691e1589d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 59d42345d86e33e01aa50013f00f4dc0612d3de8
iuc
parents: 19
diff changeset
515 difference between level 1 compression and no compression
e4691e1589d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 59d42345d86e33e01aa50013f00f4dc0612d3de8
iuc
parents: 19
diff changeset
516 -->
e4691e1589d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 59d42345d86e33e01aa50013f00f4dc0612d3de8
iuc
parents: 19
diff changeset
517 <output name="untrimmed_output" file="cutadapt_untrimmed.out.gz" compare="sim_size" delta="4000" ftype="fastq.gz"/>
e4691e1589d3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 59d42345d86e33e01aa50013f00f4dc0612d3de8
iuc
parents: 19
diff changeset
518 </test>
21
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
519 <!-- same as 1st test with paired data + filter options (because of discard_untrimmed no comparison is done) -->
23
c4b82dce8335 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit d1e907795fa943b505723dd6f7bf0a61723d8818"
iuc
parents: 22
diff changeset
520 <test expect_num_outputs="2">
21
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
521 <param name="type" value="paired" />
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
522 <param name="input_1" ftype="fastq.gz" value="bwa-mem-fastq1.fq.gz" />
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
523 <param name="input_2" ftype="fastq.gz" value="bwa-mem-fastq2.fq.gz" />
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
524 <param name="adapter_source_list" value="user"/>
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
525 <param name="adapter" value="AGATCGGAAGAGC"/>
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
526 <param name="adapter_source_list2" value="user"/>
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
527 <param name="adapter2" value="AGATCGGAAGAGC"/>
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
528 <section name="filter_options">
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
529 <param name="discard_untrimmed" value="true"/>
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
530 <param name="min" value="1"/>
104d86c98776 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 1e4c2d05b7f1052a16101cb4fc0d9ca4393e9e96"
iuc
parents: 20
diff changeset
531 <param name="max" value="1000"/>
22
093678460093 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 916aaa04992e36f9324299949b86cccd12d3081d"
iuc
parents: 21
diff changeset
532 <param name="max_n" value="0"/>
21
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533 <param name="pair_filter" value="both"/>
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534 </section>
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535 <assert_command>
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536 <has_text text="--discard-untrimmed"/>
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537 <has_text text="--minimum-length=1"/>
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538 <has_text text="--maximum-length=1000"/>
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539 <has_text text="--max-n=0"/>
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540 <has_text text="--pair-filter=both"/>
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541 </assert_command>
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542 </test>
9
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543 </tests>
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544
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545 <help><![CDATA[
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546
8665bcc8b847 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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547 .. class:: infomark
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548
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549 **What it does**
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550
8665bcc8b847 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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551 -------------------
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552
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553 **Cutadapt** finds and removes adapter sequences, primers, poly-A tails and other types of unwanted sequence from your high-throughput sequencing reads.
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554
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555 Cleaning your data in this way is often required: Reads from small-RNA sequencing contain the 3’ sequencing adapter because the read is longer than the molecule that is sequenced, such as in microRNA, or CRISPR data, or Poly-A tails that are useful for pulling out RNA from your sample but often you don’t want them to be in your reads.
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556
8665bcc8b847 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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557 Cutadapt_ helps with these trimming tasks by finding the adapter or primer sequences in an error-tolerant way. It can also modify and filter reads in various ways. Cutadapt searches for the adapter in all reads and removes it when it finds it. Unless you use a filtering option, all reads that were present in the input file will also be present in the output file, some of them trimmed, some of them not. Even reads that were trimmed entirely (because the adapter was found in the very beginning) are output. All of this can be changed with options in the tool form above.
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558
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559 The tool is based on the **Open Source** Cutadapt_ tool. See the complete `Cutadapt documentation`_ for additional details. If you use Cutadapt, please cite *Marcel, 2011* under **Citations** below.
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560
8665bcc8b847 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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561 -------------------
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562
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563 **Inputs**
8665bcc8b847 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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564
8665bcc8b847 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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565 -------------------
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566
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567 Input files for Cutadapt need to be:
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568
8665bcc8b847 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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569 - FASTQ.GZ, FASTQ.BZ2, FASTQ or FASTA
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570
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571 To trim an adapter, input the ADAPTER sequence in plain text or in a FASTA file e.g. AACCGGTT (with the characters: **$**, **^**, **...**, if anchored or linked).
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572
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573 ============================================= ===================
8665bcc8b847 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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574 **Option** **Sequence**
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575 --------------------------------------------- -------------------
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576 3’ (End) Adapter ADAPTER
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577 Anchored 3’ Adapter ADAPTER$
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578
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579 5’ (Front) Adapter ADAPTER
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580 Anchored 5’ Adapter ^ADAPTER
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581
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582 5’ or 3’ (Both possible) ADAPTER
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583
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584 Linked Adapter - 3' (End) only ADAPTER1...ADAPTER2
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585 Non-anchored Linked Adapter - 5' (Front) only ADAPTER1...ADAPTER2
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586 ============================================= ===================
8665bcc8b847 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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587
8665bcc8b847 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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588 Below is an illustration of the allowed adapter locations relative to the read and depending on the adapter type:
8665bcc8b847 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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589
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590 .. image:: $PATH_TO_IMAGES/adapters.svg
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591
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592
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593 -------------------
8665bcc8b847 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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594
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595 *Example: Illumina TruSeq Adapters*
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596
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597 -------------------
0
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Lance Parsons <lparsons@princeton.edu>
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598
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599 If you have reads containing Illumina TruSeq adapters, for example, follow these steps.
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600
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601
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602 For Single-end reads as well as the first reads of Paired-end data:
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603
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604 **Read 1**
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605
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606 In the **3' (End) Adapters** option above, insert A + the “TruSeq Indexed Adapter” prefix that is common to all Indexed Adapter sequences, e.g insert:
8665bcc8b847 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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607
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608 AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
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609
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610
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611 For the second reads of Paired-end data:
8665bcc8b847 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cutadapt commit 07c4e473990f522bbe8254ddeded47ed5f3b2fe4
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612
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613 **Read 2**
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614
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615 In the **3' (End) Adapters** option above, insert the reverse complement of the “TruSeq Universal Adapter”:
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616
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617 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT
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618
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619 The adapter sequences can be found in the document `Illumina TruSeq Adapters De-Mystified`_.
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620
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621 -----------
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622
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623 **Outputs**
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624
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625 -----------
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626
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627 - Trimmed reads
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628
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629 Optionally, under **Output Options** you can choose to output
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630
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631 * Report
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diff changeset
632 * Info file
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633
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634
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635 **Report**
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636
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637 Cutadapt can output per-adapter statistics if you select to output the report above.
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638
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639 Example:
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640
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641 *This is cutadapt 1.16 with Python 3.6.4*
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642
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643 *Command line parameters: -j 1 --format=fastq -a AGATCGGAAGAGC --info-file=/tmp/tmpX0DlY1/files/000/dataset_21.dat --output=out1.fq --error-rate=0.1 --times=1 --overlap=3 input_f.fastq*
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644 *Running on 1 core*
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645 *Trimming 1 adapter with at most 10.0% errors in single-end mode ...*
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646 *Finished in 0.00 s (1426 us/read; 0.04 M reads/minute).*
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647
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648 *=== Summary ===*
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649
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650 * Total reads processed: 3*
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651 * Reads with adapters: 0 (0.0%)*
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652 * Reads written (passing filters): 3 (100.0%)*
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653
11
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654 * Total basepairs processed: 102 bp*
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655 * Total written (filtered): 102 bp (100.0%)*
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656
11
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657 *=== Adapter 1 ===*
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658
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659 *Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 0 times.*
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660
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661
11
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662 **Info file**
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663
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664 The info file contains information about the found adapters. The output is a tab-separated text file. Each line corresponds to one read of the input file.
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665
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666 Columns contain the following data:
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667
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668 * **1st**: Read name
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669 * **2nd**: Number of errors
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670 * **3rd**: 0-based start coordinate of the adapter match
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671 * **4th**: 0-based end coordinate of the adapter match
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672 * **5th**: Sequence of the read to the left of the adapter match (can be empty)
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673 * **6th**: Sequence of the read that was matched to the adapter
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674 * **7th**: Sequence of the read to the right of the adapter match (can be empty)
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675 * **8th**: Name of the found adapter
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676 * **9th**: Quality values corresponding to sequence left of the adapter match (can be empty)
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677 * **10th**: Quality values corresponding to sequence matched to the adapter (can be empty)
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678 * **11th**: Quality values corresponding to sequence to the right of the adapter (can be empty)
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679
11
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680 The concatenation of columns 5-7 yields the full read sequence. Column 8 identifies the found adapter. Adapters without a name are numbered starting from 1. Fields 9-11 are empty if quality values are not available. Concatenating them yields the full sequence of quality values.
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681
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682 If no adapter was found, the format is as follows:
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683
11
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684 #. Read name
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685 #. The value -1
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686 #. The read sequence
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687 #. Quality values
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688
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689 When parsing the file, be aware that additional columns may be added in the future. Note also that some fields can be empty, resulting in consecutive tabs within a line.
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690
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691 If the --times option is used and greater than 1, each read can appear more than once in the info file. There will be one line for each found adapter, all with identical read names. Only for the first of those lines will the concatenation of columns 5-7 be identical to the original read sequence (and accordingly for columns 9-11). For subsequent lines, the shown sequence are the ones that were used in subsequent rounds of adapter trimming, that is, they get successively shorter.
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692
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693 --------------------
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694
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695 **More Information**
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696
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697 --------------------
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698
11
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699 See the excellent `Cutadapt documentation`_
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700
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701 .. _Cutadapt: https://cutadapt.readthedocs.io/en/stable/
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702 .. _`Cutadapt documentation`: https://cutadapt.readthedocs.io/en/latest/index.html
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703 .. _`Illumina TruSeq Adapters De-Mystified`: http://tucf-genomics.tufts.edu/documents/protocols/TUCF_Understanding_Illumina_TruSeq_Adapters.pdf
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704
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705
11
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706 --------------------
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707
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708 **Galaxy Wrapper Development**
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709
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710 --------------------
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711
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712 Author: Lance Parsons <lparsons@princeton.edu>
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713
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714 ]]></help>
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715
10
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716 <citations>
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717 <citation type="bibtex">
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718 @article{marcel_cutadapt_2011,
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719 title = {Cutadapt removes adapter sequences from high-throughput sequencing reads},
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720 volume = {17},
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721 copyright = {Authors who publish with this journal agree to the following terms: Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access ).},
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722 url = {http://journal.embnet.org/index.php/embnetjournal/article/view/200},
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723 abstract = {When small RNA is sequenced on current sequencing machines, the resulting reads are usually longer than the RNA and therefore contain parts of the 3' adapter. That adapter must be found and removed error-tolerantly from each read before read mapping. Previous solutions are either hard to use or do not offer required features, in particular support for color space data. As an easy to use alternative, we developed the command-line tool cutadapt, which supports 454, Illumina and SOLiD (color space) data, offers two adapter trimming algorithms, and has other useful features.
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724
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725 Cutadapt, including its MIT-licensed source code, is available for download at http://code.google.com/p/cutadapt/},
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726 number = {1},
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727 urldate = {2011-08-02},
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728 journal = {EMBnet.journal},
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729 author = {Marcel, Martin},
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730 year = {2011},
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731 note = {When small RNA is sequenced on current sequencing machines, the resulting reads are usually longer than the RNA and therefore contain parts of the 3' adapter. That adapter must be found and removed error-tolerantly from each read before read mapping. Previous solutions are either hard to use or do not offer required features, in particular support for color space data. As an easy to use alternative, we developed the command-line tool cutadapt, which supports 454, Illumina and SOLiD (color space) data, offers two adapter trimming algorithms, and has other useful features. Cutadapt, including its MIT-licensed source code, is available for download at http://code.google.com/p/cutadapt/},
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732 keywords = {Adapter removal;, fastq, MicroRNA, Sequencing, Small RNA, software},
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733 file = {Cutadapt removes adapter sequences from high-throughput sequencing reads | Martin | EMBnet.journal:/Users/lparsons/Library/Application Support/Firefox/Profiles/thd2t4je.default/zotero/storage/ZXZT4PSE/200.html:text/html}
01d94df2e32a planemo upload for repository https://bitbucket.org/lance_parsons/cutadapt_galaxy_wrapper
lparsons
parents: 9
diff changeset
734 }
01d94df2e32a planemo upload for repository https://bitbucket.org/lance_parsons/cutadapt_galaxy_wrapper
lparsons
parents: 9
diff changeset
735 </citation>
01d94df2e32a planemo upload for repository https://bitbucket.org/lance_parsons/cutadapt_galaxy_wrapper
lparsons
parents: 9
diff changeset
736 </citations>
01d94df2e32a planemo upload for repository https://bitbucket.org/lance_parsons/cutadapt_galaxy_wrapper
lparsons
parents: 9
diff changeset
737
0
8b064ea16722 Initial version with multiple adapter support
Lance Parsons <lparsons@princeton.edu>
parents:
diff changeset
738 </tool>