annotate bio_hansel.xml @ 5:6396d043667b draft

planemo upload for repository https://github.com/phac-nml/bio_hansel commit f9ecb9b31d5cace7e1a1ded13dd2e5eb8ef7759e-dirty
author nml
date Wed, 21 Feb 2018 12:13:32 -0500
parents c0d08ba0c6ee
children 5a42e475436e
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1 <tool id="bio_hansel" name="Bio Hansel" version="1.2.0">
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2 <description>SNV Subtyping with genome assemblies or reads</description>
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3 <requirements>
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4 <requirement type="package" version="1.2.0">bio_hansel</requirement>
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5 <requirement type="package" version="17.2.0">attrs</requirement>
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6 </requirements>
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7 <command detect_errors="exit_code"><![CDATA[
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8 ## Preparing file input.
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9 #if $data_type.type == "paired":
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5
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11 ln -s '$data_type.fastq_input1' '$data_type.fastq_input1.name'.fastq &&
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12 ln -s '$data_type.fastq_input2' '$data_type.fastq_input2.name'.fastq &&
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14 #elif $data_type.type == "collection":
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5
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16 ln -s '$data_type.fastq_input1.forward' '$data_type.fastq_input1.name'_1.fastq &&
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17 ln -s '$data_type.fastq_input1.reverse' '$data_type.fastq_input1.name'_2.fastq &&
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19 #elif $data_type.type == "single":
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1
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21 #if $data_type.fastq_input1.is_of_type('fastqsanger') or $data_type.fastq_input1.is_of_type('fastq'):
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22 ln -s '$data_type.fastq_input1' '$data_type.fastq_input1.name'.fastq &&
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23 #end if
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25 #if $data_type.fastq_input1.is_of_type('fasta'):
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26 ln -s '$data_type.fastq_input1' '$data_type.fastq_input1.name'.fasta &&
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27 #end if
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29 #end if
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32 ## Checking for custom scheme.
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33 #if $type_of_scheme.scheme_type == "custom":
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34 #if $type_of_scheme.scheme_input.is_of_type('fasta'):
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35 ln -s '$type_of_scheme.scheme_input' '$type_of_scheme.scheme_input.name' &&
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36 #end if
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37 #end if
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39 ## Start the actual command here
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40 hansel
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43 ## Select the scheme
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44 -s
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46 #if $type_of_scheme.scheme_type == "heidelberg":
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47 heidelberg
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48 #elif $type_of_scheme.scheme_type == "enteritidis":
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49 enteritidis
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50 #elif $type_of_scheme.scheme_type == "custom":
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51 '$type_of_scheme.scheme_input.name'
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52 #end if
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53
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54 ## Use Json output
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55 #if $dev_args.use_json == "yes":
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56 --json
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57 #end if
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58
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59 #if $kmer_vals.kmer_min
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60 --min-kmer-freq $kmer_vals.kmer_min
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61 #end if
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63 #if $kmer_vals.kmer_max
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64 --max-kmer-freq $kmer_vals.kmer_max
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65 #end if
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66
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67 #if $qc_vals.low_cov_depth_freq
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68 --low-cov-depth-freq $qc_vals.low_cov_depth_freq
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69 #end if
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71 #if $qc_vals.max_missing_tiles
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72 --max-missing-tiles $qc_vals.max_missing_tiles
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73 #end if
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75 #if $qc_vals.min_ambiguous_tiles
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76 --min-ambiguous-tiles $qc_vals.min_ambiguous_tiles
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77 #end if
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79 #if $qc_vals.max_intermediate_tiles
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80 --max-intermediate-tiles $qc_vals.max_intermediate_tiles
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81 #end if
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82
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83 ## Adding more parameters to the command.
3
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84 -vvv -t "\${GALAXY_SLOTS:-1}" -o results.tab -O match_results.tab -S tech_results.tab
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86
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87 ## Entering the file inputs
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88
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89 #if $data_type.type == "single":
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91 #if $data_type.fastq_input1.is_of_type('fastqsanger') or $data_type.fastq_input1.is_of_type('fastq'):
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92 '$data_type.fastq_input1.name'.fastq
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93 #end if
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95 #if $data_type.fastq_input1.is_of_type('fasta'):
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96 '$data_type.fastq_input1.name'.fasta
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97 #end if
0
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98
1
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99 #elif $data_type.type =="collection":
5
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100 -p '$data_type.fastq_input1.name'_1.fastq '$data_type.fastq_input1.name'_2.fastq
1
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101
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102 #elif $data_type.type =="paired":
5
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103 -p '$data_type.fastq_input1.name'.fastq '$data_type.fastq_input2.name'.fastq
0
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104
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105 #end if
1
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106
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107
0
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108 ]]></command>
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109 <inputs>
1
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110 <conditional name="data_type">
0
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111 <param name="type" type="select" label="Specify the read type.">
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112 <option value="single">Single-end Data</option>
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113 <option value="paired">Paired-end Data</option>
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114 <option value="collection">Collection Paired-end Data</option>
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115 </param>
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116 <when value="single">
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117 <param name="fastq_input1" type="data" format="fastqsanger, fastq, fasta" label="Single end read file(s)"/>
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118 </when>
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119 <when value="paired">
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120 <param name="fastq_input1" type="data" format="fastqsanger, fastq" label="Forward paired-end read file"/>
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121 <param name="fastq_input2" type="data" format="fastqsanger, fastq" label="Reverse paired-end read file"/>
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122 </when>
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123 <when value="collection">
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124 <param name="fastq_input1" type="data_collection" label="Paired-end reads collection" optional="false" format="fastqsanger, fastq" collection_type="paired" />
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125 </when>
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126 </conditional>
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127 <conditional name="type_of_scheme">
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128 <param name="scheme_type" type="select" label="Specify scheme to use. (Heidelberg is default)">
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129 <option value="heidelberg">Heidelberg scheme</option>
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130 <option value="enteritidis">Enteritidis scheme</option>
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131 <option value="custom">Specify your own custom scheme</option>
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132 </param>
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133 <when value="heidelberg"/>
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134 <when value="enteritidis"/>
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135 <when value="custom">
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136 <param name="scheme_input" type="data" format="fasta" label="Scheme Input"/>
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137 </when>
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138 </conditional>
3
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139 <!-- K-mer frequencies. -->
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140 <section name="kmer_vals" title="K-mer Frequency Thresholds" expanded="False">
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141 <param name="kmer_min" argument="--min-kmer-freq" optional="True" type="integer" min="0" label="Min k-mer freq/coverage" value="8" help="default = 8"/>
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142 <param name="kmer_max" argument="--max-kmer-freq" optional="True" type="integer" min="1" label="Max k-mer freq/coverage" value="1000" help="default = 1000"/>
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143 </section>
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144 <!-- Quality Checking Parameters -->
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145 <section name="qc_vals" title="Quality Checking Thresholds" expanded="False">
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146 <param name="low_cov_depth_freq" argument="--low-cov-depth-freq" optional="True" type="integer" min="0" label="QC: Frequency below this coverage are considered low coverage" value="20" help="default = 20"/>
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147 <param name="min_ambiguous_tiles" argument="--min-ambiguous-tiles" optional="True" type="integer" min="0" label="QC: Min number of tiles missing for Ambiguous Result" value="3" help="default = 3"/>
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148 <param name="max_missing_tiles" argument="--max-missing-tiles" optional="True" type="float" min="0" max="1" label="QC: Decimal Proportion of max allowed missing tiles" value="0.05" help="default = 0.05, valid values {0.0 - 1.0}"/>
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149 <param name="max_intermediate_tiles" argument="--max-intermediate-tiles" optional="True" type="float" min="0" max="1" label="QC: Decimal Proportion of max allowed missing tiles for an intermediate subtype" value="0.05" help="default = 0.05, valid values {0.0 - 1.0}"/>
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150 </section>
5
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151 <section name="dev_args" title="Developer Options" expanded="False">
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152 <param name="use_json" type="select" label="Enable JSON output" help="Use this option of you need json representations of analysis' details">
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153 <option value="no">no</option>
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154 <option value="yes">yes</option>
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155 </param>
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156 </section>
0
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157 </inputs>
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158 <outputs>
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159 <data format="tabular" name="results.tab" from_work_dir="results.tab" label="results.tab"/>
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160 <data format="tabular" name="match_results.tab" from_work_dir="match_results.tab" label="match_results.tab"/>
3
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161 <data format="tabular" name="tech_results.tab" from_work_dir="tech_results.tab" label="tech_results.tab"/>
5
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162 <data format="json" name="results.json" from_work_dir="results.tab.json" label="results.json">
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163 <filter>dev_args['use_json'] == "yes"</filter>
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164 </data>
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165 <data format="json" name="match_results.json" from_work_dir="match_results.tab.json" label="match_results.json">
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166 <filter>dev_args['use_json'] == "yes"</filter>
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167 </data>
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168 <data format="json" name="tech_results.json" from_work_dir="tech_results.tab.json" label="tech_results.json">
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169 <filter>dev_args['use_json'] == "yes"</filter>
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170 </data>
0
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171 </outputs>
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172 <tests>
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173 <test>
1
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174 <param name="type" value="single"/>
0
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175 <param name="type_of_scheme" value="heidelberg"/>
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176 <param name="fastq_input1" value="SRR1002850_SMALL.fasta"/>
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177 <output name="results.tab">
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178 <assert_contents>
5
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179 <has_text_matching expression="sample\s+scheme\s+scheme_version\s+subtype\s+all_subtypes\s+tiles_matching_subtype\s+are_subtypes_consistent\s+n_tiles_matching_all\s+n_tiles_matching_all_expected\s+n_tiles_matching_positive\s+n_tiles_matching_positive_expected\s+n_tiles_matching_subtype\s+n_tiles_matching_subtype_expected\s+file_path\s+qc_status\s+qc_message"/>
0
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180 </assert_contents>
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181 </output>
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182 <output name="match_results.tab">
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183 <assert_contents>
3
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184 <has_text_matching expression="tilename\s+seq\s+is_revcomp\s+contig_id\s+match_index\s+refposition\s+subtype\s+is_pos_tile\s+sample\s+file_path\s+scheme\s+scheme_version\s+qc_status\s+qc_message"/>
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185 </assert_contents>
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186 </output>
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187 <output name="tech_results.tab">
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188 <assert_contents>
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189 <has_text_matching expression="sample\s+subtype\s+qc_status\s+qc_message"/>
0
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190 </assert_contents>
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191 </output>
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192 </test>
1
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193 <test>
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194 <param name="type" value="paired"/>
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195 <param name="type_of_scheme" value="heidelberg"/>
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196 <param name="fastq_input1" value="SRR5646583_SMALL_1.fastq"/>
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197 <param name="fastq_input2" value="SRR5646583_SMALL_2.fastq"/>
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198 <output name="results.tab">
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199 <assert_contents>
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200 <has_text_matching expression="sample\s+scheme\s+scheme_version\s+subtype\s+all_subtypes\s+tiles_matching_subtype\s+are_subtypes_consistent\s+n_tiles_matching_all\s+n_tiles_matching_all_expected\s+n_tiles_matching_positive\s+n_tiles_matching_positive_expected\s+n_tiles_matching_subtype\s+n_tiles_matching_subtype_expected\s+file_path"/>
3
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201 </assert_contents>
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202 </output>
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203 <output name="match_results.tab">
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204 <assert_contents>
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205 <has_text_matching expression="tilename\s+seq\s+freq\s+refposition\s+subtype\s+is_pos_tile\s+is_kmer_freq_okay\s+sample\s+scheme\s+scheme_version\s+qc_status\s+qc_message"/>
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206 </assert_contents>
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207 </output>
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208 <output name="tech_results.tab">
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209 <assert_contents>
5
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210 <has_text_matching expression="sample\s+subtype\s+avg_tile_coverage\s+qc_status\s+qc_message"/>
1
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211 </assert_contents>
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212 </output>
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213 </test>
0
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214 </tests>
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215 <help><![CDATA[
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216 ***********************************************************
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217 bio_hansel - Heidelberg And eNteritidis Snp ELucidation
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218 ***********************************************************
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219
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220 Subtype *Salmonella enterica* subsp. enterica serovar Heidelberg and Enteritidis genomes using *in-silico* 33 bp k-mer SNP subtyping schemes developed by Genevieve Labbe et al.
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221 Subtype *Salmonella* genome assemblies (FASTA files) and/or whole-genome sequencing reads (FASTQ files)!
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222
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223 Usage
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224 =====
1
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225 1) Enter your FASTA/FASTQ file(s)
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226 2) Select which scheme you would like to use (e.g. heidelberg, enteritidis, or specify your own)
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227 3) Click Execute
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228
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229 For more information visit `https://github.com/phac-nml/bio_hansel`
0
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230
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231
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232 Example Usage
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233 =============
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234
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235 Analysis of a single FASTA file
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236 -------------------------------
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237
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238
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239 Contents of ``results.tab``:
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240
1
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241 +------------+------------+-------------+------------------------------------------------+---------------------------------------------------------------+-------------------------+-----------------------+----------------------+----------------------------+---------------------------+---------------------------------+--------------------------+--------------------------------+------------+
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242 | sample | scheme | subtype | all_subtypes | tiles_matching_subtype | are_subtypes_consistent | inconsistent_subtypes | n_tiles_matching_all | n_tiles_matching_all_total | n_tiles_matching_positive | n_tiles_matching_positive_total | n_tiles_matching_subtype | n_tiles_matching_subtype_total | file_path |
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243 +------------+------------+-------------+------------------------------------------------+---------------------------------------------------------------+-------------------------+-----------------------+----------------------+----------------------------+---------------------------+---------------------------------+--------------------------+--------------------------------+------------+
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244 | file.fasta | heidelberg | 2.2.2.2.1.4 | 2; 2.2; 2.2.2; 2.2.2.2; 2.2.2.2.1; 2.2.2.2.1.4 | 1037658-2.2.2.2.1.4; 2154958-2.2.2.2.1.4; 3785187-2.2.2.2.1.4 | True | | 202 | 202 | 17 | 17 | 3 | 3 | file.fasta |
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245 +------------+------------+-------------+------------------------------------------------+---------------------------------------------------------------+-------------------------+-----------------------+----------------------+----------------------------+---------------------------+---------------------------------+--------------------------+--------------------------------+------------+
0
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246
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247
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248
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249 Contents of ``match_results.tab``:
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250
1
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251 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+
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252 | tilename | stitle | pident | length | mismatch | gapopen | qstart | qend | sstart | send | evalue | bitscore | qlen | slen | seq | coverage | is_trunc | refposition | subtype | is_pos_tile | sample | file_path | scheme |
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253 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+
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254 | 775920-2.2.2.2 | NODE_2_length_512016_cov_46.4737_ID_3 | 100 | 33 | 0 | 0 | 1 | 33 | 474875 | 474907 | 2E-11 | 62.1 | 33 | 512016 | GTTCAGGTGCTACCGAGGATCGTTTTTGGTGCG | 1 | False | 775920 | 2.2.2.2 | True | out | file.fasta | heidelberg |
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255 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+
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256 | negative3305400-2.1.1.1 | NODE_3_length_427905_cov_48.1477_ID_5 | 100 | 33 | 0 | 0 | 1 | 33 | 276235 | 276267 | 2E-11 | 62.1 | 33 | 427905 | CATCGTGAAGCAGAACAGACGCGCATTCTTGCT | 1 | False | negative3305400 | 2.1.1.1 | False | out | file.fasta | heidelberg |
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257 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+
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258 | negative3200083-2.1 | NODE_3_length_427905_cov_48.1477_ID_5 | 100 | 33 | 0 | 0 | 1 | 33 | 170918 | 170950 | 2E-11 | 62.1 | 33 | 427905 | ACCCGGTCTACCGCAAAATGGAAAGCGATATGC | 1 | False | negative3200083 | 2.1 | False | out | file.fasta | heidelberg |
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259 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+
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260 | negative3204925-2.2.3.1.5 | NODE_3_length_427905_cov_48.1477_ID_5 | 100 | 33 | 0 | 0 | 1 | 33 | 175760 | 175792 | 2E-11 | 62.1 | 33 | 427905 | CTCGCTGGCAAGCAGTGCGGGTACTATCGGCGG | 1 | False | negative3204925 | 2.2.3.1.5 | False | out | file.fasta | heidelberg |
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261 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+
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262 | negative3230678-2.2.2.1.1.1 | NODE_3_length_427905_cov_48.1477_ID_5 | 100 | 33 | 0 | 0 | 1 | 33 | 201513 | 201545 | 2E-11 | 62.1 | 33 | 427905 | AGCGGTGCGCCAAACCACCCGGAATGATGAGTG | 1 | False | negative3230678 | 2.2.2.1.1.1 | False | out | file.fasta | heidelberg |
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263 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+
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264 | negative3233869-2.1.1.1.1 | NODE_3_length_427905_cov_48.1477_ID_5 | 100 | 33 | 0 | 0 | 1 | 33 | 204704 | 204736 | 2E-11 | 62.1 | 33 | 427905 | CAGCGCTGGTATGTGGCTGCACCATCGTCATTA | 1 | False | negative3233869 | 2.1.1.1.1 | False | out | file.fasta | heidelberg |
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265 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+
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266 | negative3254229-2.2.3.1.3 | NODE_3_length_427905_cov_48.1477_ID_5 | 100 | 33 | 0 | 0 | 1 | 33 | 225064 | 225096 | 2E-11 | 62.1 | 33 | 427905 | CGCCACCACGCGGTTAGCGTCACGCTGACATTC | 1 | False | negative3254229 | 2.2.3.1.3 | False | out | file.fasta | heidelberg |
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267 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+
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268 | negative3257074-2.2.1 | NODE_3_length_427905_cov_48.1477_ID_5 | 100 | 33 | 0 | 0 | 1 | 33 | 227909 | 227941 | 2E-11 | 62.1 | 33 | 427905 | CGGCAACCAGACCGACTACGCCGCCAAGCAGAC | 1 | False | negative3257074 | 2.2.1 | False | out | file.fasta | heidelberg |
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269 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+
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270 | negative3264474-2.2.2.1.1.1 | NODE_3_length_427905_cov_48.1477_ID_5 | 100 | 33 | 0 | 0 | 1 | 33 | 235309 | 235341 | 2E-11 | 62.1 | 33 | 427905 | AATGGCGCCGATCGTCGCCAGATAACCGTTGCC | 1 | False | negative3264474 | 2.2.2.1.1.1 | False | out | file.fasta | heidelberg |
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271 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+
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272 | negative3267927-2.2.2.2.2.1 | NODE_3_length_427905_cov_48.1477_ID_5 | 100 | 33 | 0 | 0 | 1 | 33 | 238762 | 238794 | 2E-11 | 62.1 | 33 | 427905 | AAAGAGAAATATGATGCCAGGCTGATACATGAC | 1 | False | negative3267927 | 2.2.2.2.2.1 | False | out | file.fasta | heidelberg |
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273 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+
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274 | negative3278067-1.1 | NODE_3_length_427905_cov_48.1477_ID_5 | 100 | 33 | 0 | 0 | 1 | 33 | 248902 | 248934 | 2E-11 | 62.1 | 33 | 427905 | TGTGAGTAAGTTGCGCGATATTCTGCTGGATTC | 1 | False | negative3278067 | 1.1 | False | out | file.fasta | heidelberg |
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275 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+
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276 | negative3299717-2.2.3.1.4 | NODE_3_length_427905_cov_48.1477_ID_5 | 100 | 33 | 0 | 0 | 1 | 33 | 270552 | 270584 | 2E-11 | 62.1 | 33 | 427905 | ATGCCGGACAGCAGGCGAAACTCGAACCGGATA | 1 | False | negative3299717 | 2.2.3.1.4 | False | out | file.fasta | heidelberg |
ba271365095e planemo upload for repository https://github.com/phac-nml/bio_hansel commit a0204b99a722240fe9b03b78a0786b30aa8ecc96
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277 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+
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278 | negative3373069-2.2.2.2.1.1 | NODE_3_length_427905_cov_48.1477_ID_5 | 100 | 33 | 0 | 0 | 1 | 33 | 344011 | 344043 | 2E-11 | 62.1 | 33 | 427905 | CTCTCCAGAAGATGAAGCCCGTGATGCGGCGCA | 1 | False | negative3373069 | 2.2.2.2.1.1 | False | out | file.fasta | heidelberg |
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279 +-----------------------------+---------------------------------------+--------+--------+----------+---------+--------+------+--------+--------+--------+----------+------+--------+-----------------------------------+----------+----------+-----------------+-------------+-------------+--------+------------+------------+
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280
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281 Next 196 lines omitted.
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282
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283
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284
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285 Analysis of a single FASTQ readset
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286 ----------------------------------
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287
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288 Contents of ``results.tab``:
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289
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290 +--------+------------+-------------+------------------------------------------------+------------------------------------------+-------------------------+-----------------------+----------------------+----------------------------+---------------------------+---------------------------------+--------------------------+--------------------------------+------------------------------------------+
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291 | sample | scheme | subtype | all_subtypes | tiles_matching_subtype | are_subtypes_consistent | inconsistent_subtypes | n_tiles_matching_all | n_tiles_matching_all_total | n_tiles_matching_positive | n_tiles_matching_positive_total | n_tiles_matching_subtype | n_tiles_matching_subtype_total | file_path |
ba271365095e planemo upload for repository https://github.com/phac-nml/bio_hansel commit a0204b99a722240fe9b03b78a0786b30aa8ecc96
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292 +--------+------------+-------------+------------------------------------------------+------------------------------------------+-------------------------+-----------------------+----------------------+----------------------------+---------------------------+---------------------------------+--------------------------+--------------------------------+------------------------------------------+
ba271365095e planemo upload for repository https://github.com/phac-nml/bio_hansel commit a0204b99a722240fe9b03b78a0786b30aa8ecc96
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293 | 564 | heidelberg | 2.2.1.1.1.1 | 2; 2.2; 2.2.1; 2.2.1.1; 2.2.1.1.1; 2.2.1.1.1.1 | 1983064-2.2.1.1.1.1; 4211912-2.2.1.1.1.1 | True | | 202 | 202 | 20 | 20 | 2 | 2 | forward.fastqsanger; reverse.fastqsanger |
ba271365095e planemo upload for repository https://github.com/phac-nml/bio_hansel commit a0204b99a722240fe9b03b78a0786b30aa8ecc96
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294 +--------+------------+-------------+------------------------------------------------+------------------------------------------+-------------------------+-----------------------+----------------------+----------------------------+---------------------------+---------------------------------+--------------------------+--------------------------------+------------------------------------------+
0
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295
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297 Contents of ``match_results.tab``:
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298
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299 +-----------------------------------+------+--------+------------------------------------------+------------------+-------------+-----------+-------------+-------------------+------------+
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300 | seq | freq | sample | file_path | tilename | is_pos_tile | subtype | refposition | is_kmer_freq_okay | scheme |
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301 +-----------------------------------+------+--------+------------------------------------------+------------------+-------------+-----------+-------------+-------------------+------------+
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302 | ACGGTAAAAGAGGACTTGACTGGCGCGATTTGC | 68 | 564 | forward.fastqsanger; reverse.fastqsanger | 21097-2.2.1.1.1 | True | 2.2.1.1.1 | 21097 | True | heidelberg |
ba271365095e planemo upload for repository https://github.com/phac-nml/bio_hansel commit a0204b99a722240fe9b03b78a0786b30aa8ecc96
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303 +-----------------------------------+------+--------+------------------------------------------+------------------+-------------+-----------+-------------+-------------------+------------+
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304 | AACCGGCGGTATTGGCTGCGGTAAAAGTACCGT | 77 | 564 | forward.fastqsanger; reverse.fastqsanger | 157792-2.2.1.1.1 | True | 2.2.1.1.1 | 157792 | True | heidelberg |
ba271365095e planemo upload for repository https://github.com/phac-nml/bio_hansel commit a0204b99a722240fe9b03b78a0786b30aa8ecc96
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305 +-----------------------------------+------+--------+------------------------------------------+------------------+-------------+-----------+-------------+-------------------+------------+
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306 | CCGCTGCTTTCTGAAATCGCGCGTCGTTTCAAC | 67 | 564 | forward.fastqsanger; reverse.fastqsanger | 293728-2.2.1.1 | True | 2.2.1.1 | 293728 | True | heidelberg |
ba271365095e planemo upload for repository https://github.com/phac-nml/bio_hansel commit a0204b99a722240fe9b03b78a0786b30aa8ecc96
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307 +-----------------------------------+------+--------+------------------------------------------+------------------+-------------+-----------+-------------+-------------------+------------+
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308 | GAATAACAGCAAAGTGATCATGATGCCGCTGGA | 91 | 564 | forward.fastqsanger; reverse.fastqsanger | 607438-2.2.1 | True | 2.2.1 | 607438 | True | heidelberg |
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309 +-----------------------------------+------+--------+------------------------------------------+------------------+-------------+-----------+-------------+-------------------+------------+
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310 | CAGTTTTACATCCTGCGAAATGCGCAGCGTCAA | 87 | 564 | forward.fastqsanger; reverse.fastqsanger | 691203-2.2.1.1 | True | 2.2.1.1 | 691203 | True | heidelberg |
ba271365095e planemo upload for repository https://github.com/phac-nml/bio_hansel commit a0204b99a722240fe9b03b78a0786b30aa8ecc96
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311 +-----------------------------------+------+--------+------------------------------------------+------------------+-------------+-----------+-------------+-------------------+------------+
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312 | CAGGAGAAAGGATGCCAGGGTCAACACGTAAAC | 33 | 564 | forward.fastqsanger; reverse.fastqsanger | 944885-2.2.1.1.1 | True | 2.2.1.1.1 | 944885 | True | heidelberg |
ba271365095e planemo upload for repository https://github.com/phac-nml/bio_hansel commit a0204b99a722240fe9b03b78a0786b30aa8ecc96
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313 +-----------------------------------+------+--------+------------------------------------------+------------------+-------------+-----------+-------------+-------------------+------------+
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314
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315 Next 200 lines omitted.
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316
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317 Galaxy wrapper written by Matthew Gopez at the Public Health Agency of Canada, National Microbiology Laboratory.
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318
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319 ]]></help>
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320 <citations>
1
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321 <citation type="bibtex">@ARTICLE{a1,
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322 title = {A robust genotyping scheme for *Salmonella enterica* serovar Heidelberg clones circulating in North America.},
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323 author = {Geneviève Labbé, James Robertson, Peter Kruczkiewicz, Chad R. Laing, Kim Ziebell, Aleisha R. Reimer, Lorelee Tschetter, Gary Van Domselaar, Sadjia Bekal, Kimberley A. MacDonald, Linda Hoang, Linda Chui, Danielle Daignault, Durda Slavic, Frank Pollari, E. Jane Parmley, Philip Mabon, Elissa Giang, Lok Kan Lee, Jonathan Moffat, Marisa Rankin, Joanne MacKinnon, Roger Johnson, John H.E. Nash.},
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324 url = {https://github.com/phac-nml/bio_hansel}
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325 }
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326 }</citation>
0
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327 </citations>
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328 </tool>