annotate staramr_search.xml @ 11:5f43703968cf draft

"planemo upload for repository https://github.com/phac-nml/galaxy_tools/tree/master/tools/staramr commit 65ffe1cd11ac6108adf483d3e83856a5466df79c"
author nml
date Wed, 18 Dec 2019 16:14:07 -0500
parents 7573a6c11c4d
children aeaa20b96ef2
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1 <tool id="staramr_search" name="staramr" version="@VERSION@">
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2 <description>Scans genome assemblies against the ResFinder, PlasmidFinder, and PointFinder databases searching for AMR genes.</description>
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3 <macros>
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4 <token name="@VERSION@">0.7.1</token>
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5 </macros>
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6 <requirements>
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7 <requirement type="package" version="@VERSION@">staramr</requirement>
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8 </requirements>
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9 <version_command>staramr --version</version_command>
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10 <command detect_errors="exit_code"><![CDATA[
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11 #import re
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13 #set $named_genomes = ''
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14 #for $genome in $genomes
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15 #set $_named_genome = re.sub(r'(\s|\(|\)|\/)', '_', '"{}.fasta"'.format($genome.element_identifier))
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16 ln -s '$genome' $_named_genome &&
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17 #set $named_genomes = $named_genomes + ' ' + $_named_genome
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18 #end for
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20 #set $excel_proper_extension = '"{}.xlsx"'.format($excel)
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21 ln -s "$excel" $excel_proper_extension &&
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23 staramr search
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25 --nprocs "\${GALAXY_SLOTS:-1}"
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26
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27 --genome-size-lower-bound $advanced.genome_size_lower_bound
7573a6c11c4d "planemo upload for repository https://github.com/phac-nml/galaxy_tools/tree/master/tools/staramr commit 69b939934ff9f503c7314cdbe6e3cb08e5f84db5"
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28 --genome-size-upper-bound $advanced.genome_size_upper_bound
7573a6c11c4d "planemo upload for repository https://github.com/phac-nml/galaxy_tools/tree/master/tools/staramr commit 69b939934ff9f503c7314cdbe6e3cb08e5f84db5"
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29 --minimum-N50-value $advanced.minimum_N50_value
7573a6c11c4d "planemo upload for repository https://github.com/phac-nml/galaxy_tools/tree/master/tools/staramr commit 69b939934ff9f503c7314cdbe6e3cb08e5f84db5"
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30 --minimum-contig-length $advanced.minimum_contig_length
7573a6c11c4d "planemo upload for repository https://github.com/phac-nml/galaxy_tools/tree/master/tools/staramr commit 69b939934ff9f503c7314cdbe6e3cb08e5f84db5"
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31 --unacceptable-number-contigs $advanced.unacceptable_number_contigs
7573a6c11c4d "planemo upload for repository https://github.com/phac-nml/galaxy_tools/tree/master/tools/staramr commit 69b939934ff9f503c7314cdbe6e3cb08e5f84db5"
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32
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33 --pid-threshold $advanced.pid_threshold
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34 --percent-length-overlap-resfinder $advanced.plength_resfinder
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35 --percent-length-overlap-plasmidfinder $advanced.plength_plasmidfinder
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36 --percent-length-overlap-pointfinder $advanced.plength_pointfinder
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38 $advanced.report_all_blast
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39 $advanced.exclude_negatives
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40 $advanced.exclude_resistance_phenotypes
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41
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42 #if str($advanced.exclude_genes.exclude_genes_condition) == 'custom'
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43 --exclude-genes-file '${advanced.exclude_genes.exclude_genes_file}'
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44 #elif str($advanced.exclude_genes.exclude_genes_condition) == 'none'
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45 --no-exclude-genes
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46 #end if
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47
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48 #if str($advanced.plasmidfinder_type) != 'include_all'
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49 --plasmidfinder-database-type '${advanced.plasmidfinder_type}'
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50 #end if
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51
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52 --output-summary $summary
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53 --output-detailed-summary $detailed_summary
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54 --output-resfinder $resfinder
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55 --output-plasmidfinder $plasmidfinder
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56 #if str($use_pointfinder) != 'disabled'
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57 --output-pointfinder $pointfinder
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58 #end if
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59 --output-settings $settings
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60 --output-excel $excel_proper_extension
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62 --output-hits-dir staramr_hits
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63
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64 #if str($use_pointfinder) != 'disabled'
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65 --pointfinder-organism '${use_pointfinder}'
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66 #end if
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67
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68 $named_genomes
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69 ]]></command>
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70 <inputs>
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71 <param type="data" name="genomes" format="fasta" multiple="true"/>
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72 <param name="use_pointfinder" type="select" label="Enable scanning for point mutations using the PointFinder database">
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73 <option value="disabled" selected="true">Disable PointFinder</option>
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74 <option value="salmonella">Salmonella</option>
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75 <option value="campylobacter">Campylobacter</option>
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76 </param>
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77 <section name="advanced" title="Advanced options" expanded="false">
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78 <param name="pid_threshold" type="float" label="Percent identity threshold for BLAST" value="98.0"
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79 min="0" max="100" help="(--pid-threshold)" />
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80 <param name="plength_resfinder" type="float" label="Percent length overlap of BLAST hit for ResFinder database" value="60.0"
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81 min="0" max="100" help="(--percent-length-overlap-resfinder)" />
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82 <param name="plength_pointfinder" type="float" label="Percent length overlap of BLAST hit for PointFinder database" value="95.0"
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83 min="0" max="100" help="(--percent-length-overlap-pointfinder)" />
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84 <param name="plength_plasmidfinder" type="float" label="Percent length overlap of BLAST hit for PlasmidFinder database" value="60.0"
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85 min="0" max="100" help="(--percent-length-overlap-plasmidfinder)" />
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7573a6c11c4d "planemo upload for repository https://github.com/phac-nml/galaxy_tools/tree/master/tools/staramr commit 69b939934ff9f503c7314cdbe6e3cb08e5f84db5"
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86 <param name="genome_size_lower_bound" type="integer" label="The lower bound for our genome size for the quality metrics" value="4000000"
7573a6c11c4d "planemo upload for repository https://github.com/phac-nml/galaxy_tools/tree/master/tools/staramr commit 69b939934ff9f503c7314cdbe6e3cb08e5f84db5"
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87 help="(--genome-size-lower-bound)" />
7573a6c11c4d "planemo upload for repository https://github.com/phac-nml/galaxy_tools/tree/master/tools/staramr commit 69b939934ff9f503c7314cdbe6e3cb08e5f84db5"
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88 <param name="genome_size_upper_bound" type="integer" label="The upper bound for our genome size for the quality metrics" value="6000000"
7573a6c11c4d "planemo upload for repository https://github.com/phac-nml/galaxy_tools/tree/master/tools/staramr commit 69b939934ff9f503c7314cdbe6e3cb08e5f84db5"
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89 help="(--genome-size-upper-bound)" />
7573a6c11c4d "planemo upload for repository https://github.com/phac-nml/galaxy_tools/tree/master/tools/staramr commit 69b939934ff9f503c7314cdbe6e3cb08e5f84db5"
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90 <param name="minimum_N50_value" type="integer" label="The minimum N50 value for the quality metrics" value="10000"
7573a6c11c4d "planemo upload for repository https://github.com/phac-nml/galaxy_tools/tree/master/tools/staramr commit 69b939934ff9f503c7314cdbe6e3cb08e5f84db5"
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91 help="(--minimum-N50-value)" />
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92 <param name="minimum_contig_length" type="integer" label="The minimum contig length for the quality metrics" value="300"
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93 help="(--minimum-contig-length)" />
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94 <param name="unacceptable_number_contigs" type="integer" label="The number of contigs, under the minimum contig length which is unacceptable for the quality metrics" value="1000"
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95 help="(--unacceptable-number-contigs)" />
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96 <param name="report_all_blast" type="boolean" label="Report all BLAST results" truevalue="--report-all-blast" falsevalue=""
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97 help="Report all BLAST results (includes overlapping hits, mainly for debugging)" />
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98 <param name="exclude_negatives" type="boolean" label="Exclude negative (non-resistant) results" truevalue="--exclude-negatives" falsevalue="" />
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99 <param name="exclude_resistance_phenotypes" type="boolean" label="Exclude resistance phenotypes" truevalue="--exclude-resistance-phenotypes" falsevalue="" />
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100 <conditional name="exclude_genes">
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101 <param name="exclude_genes_condition" type="select" label="Exclude certain AMR genes from the results">
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102 <option value="default" selected="true">Exclude default list of AMR genes</option>
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103 <option value="custom">Provide a custom list of AMR genes to exclude</option>
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104 <option value="none">Do not exclude any AMR genes</option>
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105 </param>
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106 <when value="default" />
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107 <when value="custom">
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108 <param type="data" name="exclude_genes_file" format="txt,tabular" label="Exclude genes file" help="Pass a file containing a list of genes to exclude from the ResFinder/PointFinder results" />
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109 </when>
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110 <when value="none" />
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111 </conditional>
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112 <param name="plasmidfinder_type" type="select" label="Specify PlasmidFinder Database type">
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113 <option value="include_all" selected="true">Include all available database types</option>
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114 <option value="gram_positive">Gram Positive</option>
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115 <option value="enterobacteriaceae">Enterobacteriaceae</option>
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116 </param>
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117 </section>
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118 </inputs>
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119 <outputs>
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120 <data format="tabular" name="mlst" label="${tool.name} on ${on_string}: mlst.tsv" />
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121 <data format="tabular" name="summary" label="${tool.name} on ${on_string}: summary.tsv" />
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122 <data format="tabular" name="detailed_summary" label="${tool.name} on ${on_string}: detailed_summary.tsv" />
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123 <data format="tabular" name="resfinder" label="${tool.name} on ${on_string}: resfinder.tsv" />
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124 <data format="tabular" name="plasmidfinder" label="${tool.name} on ${on_string}: plasmidfinder.tsv" />
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125 <data format="tabular" name="pointfinder" label="${tool.name} on ${on_string}: pointfinder.tsv">
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126 <filter>use_pointfinder != "disabled"</filter>
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127 </data>
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128 <data format="txt" name="settings" label="${tool.name} on ${on_string}: settings.txt" />
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129 <data format="xlsx" name="excel" label="${tool.name} on ${on_string}: results.xlsx" />
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130 <collection name="blast_hits" type="list" label="${tool.name} on ${on_string}: hits">
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131 <discover_datasets pattern="__name_and_ext__" directory="staramr_hits" />
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132 </collection>
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133 </outputs>
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134 <tests>
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135 <test>
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136 <param name="genomes" value="16S-rc_gyrA-rc_beta-lactam.fsa" />
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137 <param name="use_pointfinder" value="salmonella" />
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138
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139 <output name="summary" file="test1-summary.tsv" ftype="tabular" />
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140 <output name="detailed_summary" file="test1-detailed-summary.tsv" ftype="tabular" />
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141 <output name="resfinder" file="test1-resfinder.tsv" ftype="tabular" />
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142 <output name="plasmidfinder" file="test1-plasmidfinder.tsv" ftype="tabular" />
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143 <output name="pointfinder" file="test1-pointfinder.tsv" ftype="tabular" />
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144 <output_collection name="blast_hits" type="list">
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145 <element name="resfinder_16S-rc_gyrA-rc_beta-lactam.fsa" file="test1-hits/resfinder_16S-rc_gyrA-rc_beta-lactam.fsa" />
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146 <element name="pointfinder_16S-rc_gyrA-rc_beta-lactam.fsa" file="test1-hits/pointfinder_16S-rc_gyrA-rc_beta-lactam.fsa" />
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147 </output_collection>
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148 </test>
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149 <test>
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150 <param name="genomes" value="16S-rc_gyrA-rc_beta-lactam.fsa" />
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151
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152 <output name="summary" file="test2-summary.tsv" ftype="tabular" />
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153 <output name="resfinder" file="test2-resfinder.tsv" ftype="tabular" />
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154 <output_collection name="blast_hits" type="list">
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155 <element name="resfinder_16S-rc_gyrA-rc_beta-lactam.fsa" file="test2-hits/resfinder_16S-rc_gyrA-rc_beta-lactam.fsa" />
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156 </output_collection>
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157 </test>
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158 <test>
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159 <param name="genomes" value="16S-rc_gyrA-rc_beta-lactam.fsa" />
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160 <param name="pid_threshold" value="99.8" />
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161
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162 <output name="summary" file="test3-summary.tsv" ftype="tabular" />
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163 </test>
1
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164 <test>
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165 <param name="genomes" value="16S rc_gyrA rc_beta-lactam spaces (extra:characters).fsa" />
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166 <param name="pid_threshold" value="99.8" />
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167
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168 <output name="summary" file="test4-summary.tsv" ftype="tabular" />
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169 </test>
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170 <test>
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171 <param name="genomes" value="test-aminoglycoside.fsa" />
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172
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173 <output name="summary" file="test5-summary.tsv" ftype="tabular" />
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174 </test>
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175 <test>
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176 <param name="genomes" value="test-aminoglycoside.fsa" />
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177 <param name="exclude_genes_condition" value="none" />
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178
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179 <output name="summary" file="test6-summary.tsv" ftype="tabular" />
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180 </test>
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181 <test>
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182 <param name="genomes" value="test-aminoglycoside.fsa" />
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183 <param name="exclude_genes_condition" value="custom" />
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184 <param name="exclude_genes_file" value="genes_to_exclude.tsv" />
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185
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186 <output name="summary" file="test5-summary.tsv" ftype="tabular" />
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187 </test>
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188 <test>
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189 <param name="genomes" value="test-aminoglycoside.fsa" />
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190 <param name="genome_size_lower_bound" value="1" />
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191 <param name="genome_size_upper_bound" value="10" />
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192
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193 <output name="summary" file="test8-summary.tsv" ftype="tabular"/>
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194 </test>
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195 </tests>
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196 <help><![CDATA[
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197 staramr
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198 =======
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199
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200 staramr_ scans bacterial genome contigs against both the ResFinder_, PlasmidFinder_ and PointFinder_ databases (used by the ResFinder webservice_) and compiles a summary report of detected antimicrobial resistance genes.
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201
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202 Usage
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203 -----
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204
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205 1. Select your genome contigs (in FASTA format).
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206 2. Select whether or not you wish to scan your genome for point mutations giving antimicrobial resistance using the PointFinder database. This requires you to specify the specific organism you are scanning (currently only *salmonella* and *campylobacter* is supported).
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207 3. Run the tool.
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208
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209 Input
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210 -----
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211
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212 Genomes
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213 ```````
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214
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215 staramr_ takes as input one or more assembled genomes (in FASTA format) to search for AMR genes.
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216
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217 Exclude genes file
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218 ``````````````````
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219
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220 Setting **Provide a custom list of AMR genes to exclude** in the **Advanced options** allows you to pass a file containing a list of genes to exclude from the results. The file must start with a line **#gene_id** and the gene names must correspond to the sequence IDs in the ResFinder/PointFinder databases. For example:
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221
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222 ::
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223
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224 #gene_id
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225 aac(6')-Iaa_1_NC_003197
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226 ColpVC_1__JX133088
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227
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228 Output
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229 ------
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230
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231 There are 6 different output files produced by `staramr` as well as a collection of additional files.
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232
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233 mlst.tsv
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234 ````````````````````
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235
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236 A tabular file of each multi-locus sequence type (MLST) and it's corresponding locus/alleles, one genome per line.
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237
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238 +------------+--------------+-----------------------------------+------------+------------+------------+------------+------------+------------+------------+
61a3e97aa865 "planemo upload for repository https://github.com/phac-nml/galaxy_tools/tree/master/tools/staramr commit 07b1134fbf48f0f94e6060b861651e1ab48cde71"
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239 | Isolate ID | Scheme | Sequence Type | Locus 1 | Locus 2 | Locus 3 | Locus 4 | Locus 5 | Locus 6 | Locus 7 |
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240 +============+==============+===================================+============+============+============+============+============+============+============+
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241 | SRR1952908 | senterica | 11 | aroC(5) | dnaN(2) | hemD(3) | hisD(7) | purE(6) | sucA(6) | thrA(11) |
61a3e97aa865 "planemo upload for repository https://github.com/phac-nml/galaxy_tools/tree/master/tools/staramr commit 07b1134fbf48f0f94e6060b861651e1ab48cde71"
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242 +------------+--------------+-----------------------------------+------------+------------+------------+------------+------------+------------+---+--------+
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243 | SRR1952926 | senterica | 11 | aroC(5) | dnaN(2) | hemD(3) | hisD(7) | purE(6) | sucA(6) | thrA(11) |
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244 +------------+--------------+-----------------------------------+------------+------------+------------+------------+------------+------------+------------+
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245
0
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246
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247 summary.tsv
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248 ```````````
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249
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250 A summary of all detected AMR genes/mutations in each genome, one genome per line.
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251
11
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252 | Isolate ID | Quality Module | Genotype | Predicted Phenotype | Plasmid | Scheme | Sequence Type | Genome Length | N50 value | Number of Contigs Greater Than Or Equal To 300 bp | Quality Module Feedback |
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253 |------------|----------------|-----------------------------------------------------------|-----------------------------------------------------------------------------------------------------------|-------------------------------------|-----------|---------------|---------------|-----------|---------------------------------------------------|-------------------------|
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254 | SRR1952908 | Passed | aadA1, aadA2, blaTEM-57, cmlA1, gyrA (S83Y), sul3, tet(A) | streptomycin, ampicillin, chloramphenicol, ciprofloxacin I/R, nalidixic acid, sulfisoxazole, tetracycline | ColpVC, IncFIB(S), IncFII(S), IncI1 | senterica | 11 | 4796082 | 225419 | 59 | |
5f43703968cf "planemo upload for repository https://github.com/phac-nml/galaxy_tools/tree/master/tools/staramr commit 65ffe1cd11ac6108adf483d3e83856a5466df79c"
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255 | SRR1952926 | Passed | blaTEM-57, gyrA (S83Y), tet(A) | ampicillin, ciprofloxacin I/R, nalidixic acid, tetracycline | ColpVC, IncFIB(S), IncFII(S), IncI1 | senterica | 11 | 4794071 | 225380 | 50 | |
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256
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257
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258 detailed_summary.tsv
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259 ````````````````````
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260
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261 A detailed summary of all detected AMR genes/mutations/plasmids in each genome/type, one gene/type per line.
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262
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263 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
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264 | Isolate ID | Gene/Plasmid | Predicted Phenotype | %Identity | %Overlap | HSP Length/Total Length | Contig | Start | End | Accession | Data Type |
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265 +============+==============+===================================+===========+==========+=========================+=============+========+========+===========+============+
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266 | SRR1952908 | ColpVC | | 98.96 | 100 | 193/193 | contig00038 | 1618 | 1426 | JX133088 | Plasmid |
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267 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
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268 | SRR1952908 | IncFIB(S) | | 98.91 | 100 | 643/643 | contig00024 | 10302 | 9660 | FN432031 | Plasmid |
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269 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
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270 | SRR1952908 | IncFII(S) | | 100 | 100 | 262/262 | contig00024 | 54294 | 54555 | CP000858 | Plasmid |
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
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parents: 6
diff changeset
271 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
nml
parents: 6
diff changeset
272 | SRR1952908 | IncI1 | | 100 | 100 | 142/142 | contig00020 | 3907 | 3766 | AP005147 | Plasmid |
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
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parents: 6
diff changeset
273 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
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parents: 6
diff changeset
274 | SRR1952908 | aadA1 | streptomycin | 100 | 100 | 792/792 | contig00030 | 5355 | 4564 | JQ414041 | Resistance |
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
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parents: 6
diff changeset
275 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
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parents: 6
diff changeset
276 | SRR1952908 | aadA2 | streptomycin | 100 | 100 | 780/780 | contig00030 | 7748 | 6969 | X68227 | Resistance |
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
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parents: 6
diff changeset
277 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
nml
parents: 6
diff changeset
278 | SRR1952908 | blaTEM-57 | ampicillin | 99.88 | 100 | 861/861 | contig00032 | 6247 | 5387 | FJ405211 | Resistance |
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
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parents: 6
diff changeset
279 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
nml
parents: 6
diff changeset
280 | SRR1952908 | cmlA1 | chloramphenicol | 99.92 | 100 | 1260/1260 | contig00030 | 6707 | 5448 | M64556 | Resistance |
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
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parents: 6
diff changeset
281 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
nml
parents: 6
diff changeset
282 | SRR1952908 | gyrA (S83Y) | ciprofloxacin I/R, nalidixic acid | 99.96 | 100 | 2637/2637 | contig00008 | 22801 | 20165 | | Resistance |
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
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parents: 6
diff changeset
283 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
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parents: 6
diff changeset
284 | SRR1952908 | sul3 | sulfisoxazole | 100 | 100 | 792/792 | contig00030 | 2091 | 2882 | AJ459418 | Resistance |
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
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parents: 6
diff changeset
285 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
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parents: 6
diff changeset
286 | SRR1952908 | tet(A) | tetracycline | 99.92 | 97.8 | 1247/1275 | contig00032 | 1476 | 2722 | AF534183 | Resistance |
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
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parents: 6
diff changeset
287 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
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parents: 6
diff changeset
288 | SRR1952926 | ColpVC | | 98.96 | 100 | 193/193 | contig00037 | 657 | 849 | JX133088 | Plasmid |
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
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parents: 6
diff changeset
289 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
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parents: 6
diff changeset
290 | SRR1952926 | IncFIB(S) | | 98.91 | 100 | 643/643 | contig00021 | 10302 | 9660 | FN432031 | Plasmid |
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
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parents: 6
diff changeset
291 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
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parents: 6
diff changeset
292 | SRR1952926 | IncFII(S) | | 100 | 100 | 262/262 | contig00021 | 54294 | 54555 | CP000858 | Plasmid |
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
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parents: 6
diff changeset
293 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
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parents: 6
diff changeset
294 | SRR1952926 | IncI1 | | 100 | 100 | 142/142 | contig00017 | 3907 | 3766 | AP005147 | Plasmid |
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
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parents: 6
diff changeset
295 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
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parents: 6
diff changeset
296 | SRR1952926 | blaTEM-57 | ampicillin | 99.88 | 100 | 861/861 | contig00027 | 6176 | 5316 | FJ405211 | Resistance |
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
nml
parents: 6
diff changeset
297 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
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parents: 6
diff changeset
298 | SRR1952926 | gyrA (S83Y) | ciprofloxacin I/R, nalidixic acid | 99.96 | 100 | 2637/2637 | contig00011 | 157768 | 160404 | | Resistance |
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
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parents: 6
diff changeset
299 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
nml
parents: 6
diff changeset
300 | SRR1952926 | tet(A) | tetracycline | 99.92 | 97.8 | 1247/1275 | contig00027 | 1405 | 2651 | AF534183 | Resistance |
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
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parents: 6
diff changeset
301 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
6
3c09557ac425 planemo upload for repository https://github.com/phac-nml/staramr commit 52535d98ad1e03c83c93f230abf966613b4b8fa2
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diff changeset
302
0
fef5aff062c7 planemo upload for repository https://github.com/phac-nml/staramr commit 8aafd6d7140b7d8aeb097c80fbb7632397ba5e64
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303
fef5aff062c7 planemo upload for repository https://github.com/phac-nml/staramr commit 8aafd6d7140b7d8aeb097c80fbb7632397ba5e64
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diff changeset
304 resfinder.tsv
fef5aff062c7 planemo upload for repository https://github.com/phac-nml/staramr commit 8aafd6d7140b7d8aeb097c80fbb7632397ba5e64
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diff changeset
305 `````````````
fef5aff062c7 planemo upload for repository https://github.com/phac-nml/staramr commit 8aafd6d7140b7d8aeb097c80fbb7632397ba5e64
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306
fef5aff062c7 planemo upload for repository https://github.com/phac-nml/staramr commit 8aafd6d7140b7d8aeb097c80fbb7632397ba5e64
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diff changeset
307 A tabular file of each AMR gene and additional BLAST information from the **ResFinder** database, one gene per line.
fef5aff062c7 planemo upload for repository https://github.com/phac-nml/staramr commit 8aafd6d7140b7d8aeb097c80fbb7632397ba5e64
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308
fef5aff062c7 planemo upload for repository https://github.com/phac-nml/staramr commit 8aafd6d7140b7d8aeb097c80fbb7632397ba5e64
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diff changeset
309 +------------+------------+----------------------+------------+-----------+--------------------------+--------------+--------+-------+-----------+
fef5aff062c7 planemo upload for repository https://github.com/phac-nml/staramr commit 8aafd6d7140b7d8aeb097c80fbb7632397ba5e64
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diff changeset
310 | Isolate ID | Gene | Predicted Phenotype | %Identity | %Overlap | HSP Length/Total Length | Contig | Start | End | Accession |
fef5aff062c7 planemo upload for repository https://github.com/phac-nml/staramr commit 8aafd6d7140b7d8aeb097c80fbb7632397ba5e64
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parents:
diff changeset
311 +============+============+======================+============+===========+==========================+==============+========+=======+===========+
fef5aff062c7 planemo upload for repository https://github.com/phac-nml/staramr commit 8aafd6d7140b7d8aeb097c80fbb7632397ba5e64
nml
parents:
diff changeset
312 | SRR1952908 | sul3 | sulfisoxazole | 100.00 | 100.00 | 792/792 | contig00030 | 2091 | 2882 | AJ459418 |
fef5aff062c7 planemo upload for repository https://github.com/phac-nml/staramr commit 8aafd6d7140b7d8aeb097c80fbb7632397ba5e64
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parents:
diff changeset
313 +------------+------------+----------------------+------------+-----------+--------------------------+--------------+--------+-------+-----------+
fef5aff062c7 planemo upload for repository https://github.com/phac-nml/staramr commit 8aafd6d7140b7d8aeb097c80fbb7632397ba5e64
nml
parents:
diff changeset
314 | SRR1952908 | tet(A) | tetracycline | 99.92 | 100.00 | 1200/1200 | contig00032 | 1551 | 2750 | AJ517790 |
fef5aff062c7 planemo upload for repository https://github.com/phac-nml/staramr commit 8aafd6d7140b7d8aeb097c80fbb7632397ba5e64
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parents:
diff changeset
315 +------------+------------+----------------------+------------+-----------+--------------------------+--------------+--------+-------+-----------+
fef5aff062c7 planemo upload for repository https://github.com/phac-nml/staramr commit 8aafd6d7140b7d8aeb097c80fbb7632397ba5e64
nml
parents:
diff changeset
316 | SRR1952908 | cmlA1 | chloramphenicol | 99.92 | 100.00 | 1260/1260 | contig00030 | 6707 | 5448 | M64556 |
fef5aff062c7 planemo upload for repository https://github.com/phac-nml/staramr commit 8aafd6d7140b7d8aeb097c80fbb7632397ba5e64
nml
parents:
diff changeset
317 +------------+------------+----------------------+------------+-----------+--------------------------+--------------+--------+-------+-----------+
fef5aff062c7 planemo upload for repository https://github.com/phac-nml/staramr commit 8aafd6d7140b7d8aeb097c80fbb7632397ba5e64
nml
parents:
diff changeset
318 | SRR1952908 | aadA1 | streptomycin | 100.00 | 100.00 | 792/792 | contig00030 | 5355 | 4564 | JQ414041 |
fef5aff062c7 planemo upload for repository https://github.com/phac-nml/staramr commit 8aafd6d7140b7d8aeb097c80fbb7632397ba5e64
nml
parents:
diff changeset
319 +------------+------------+----------------------+------------+-----------+--------------------------+--------------+--------+-------+-----------+
fef5aff062c7 planemo upload for repository https://github.com/phac-nml/staramr commit 8aafd6d7140b7d8aeb097c80fbb7632397ba5e64
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parents:
diff changeset
320 | SRR1952908 | aadA2 | streptomycin | 99.75 | 100.00 | 792/792 | contig00030 | 7760 | 6969 | JQ364967 |
fef5aff062c7 planemo upload for repository https://github.com/phac-nml/staramr commit 8aafd6d7140b7d8aeb097c80fbb7632397ba5e64
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parents:
diff changeset
321 +------------+------------+----------------------+------------+-----------+--------------------------+--------------+--------+-------+-----------+
fef5aff062c7 planemo upload for repository https://github.com/phac-nml/staramr commit 8aafd6d7140b7d8aeb097c80fbb7632397ba5e64
nml
parents:
diff changeset
322 | SRR1952908 | blaTEM-57 | ampicillin | 99.88 | 100.00 | 861/861 | contig00032 | 6247 | 5387 | FJ405211 |
fef5aff062c7 planemo upload for repository https://github.com/phac-nml/staramr commit 8aafd6d7140b7d8aeb097c80fbb7632397ba5e64
nml
parents:
diff changeset
323 +------------+------------+----------------------+------------+-----------+--------------------------+--------------+--------+-------+-----------+
fef5aff062c7 planemo upload for repository https://github.com/phac-nml/staramr commit 8aafd6d7140b7d8aeb097c80fbb7632397ba5e64
nml
parents:
diff changeset
324 | SRR1952926 | tet(A) | tetracycline | 99.92 | 100.00 | 1200/1200 | contig00027 | 1480 | 2679 | AJ517790 |
fef5aff062c7 planemo upload for repository https://github.com/phac-nml/staramr commit 8aafd6d7140b7d8aeb097c80fbb7632397ba5e64
nml
parents:
diff changeset
325 +------------+------------+----------------------+------------+-----------+--------------------------+--------------+--------+-------+-----------+
fef5aff062c7 planemo upload for repository https://github.com/phac-nml/staramr commit 8aafd6d7140b7d8aeb097c80fbb7632397ba5e64
nml
parents:
diff changeset
326 | SRR1952926 | blaTEM-57 | ampicillin | 99.88 | 100.00 | 861/861 | contig00027 | 6176 | 5316 | FJ405211 |
fef5aff062c7 planemo upload for repository https://github.com/phac-nml/staramr commit 8aafd6d7140b7d8aeb097c80fbb7632397ba5e64
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parents:
diff changeset
327 +------------+------------+----------------------+------------+-----------+--------------------------+--------------+--------+-------+-----------+
fef5aff062c7 planemo upload for repository https://github.com/phac-nml/staramr commit 8aafd6d7140b7d8aeb097c80fbb7632397ba5e64
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diff changeset
328
6
3c09557ac425 planemo upload for repository https://github.com/phac-nml/staramr commit 52535d98ad1e03c83c93f230abf966613b4b8fa2
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diff changeset
329 plasmidfinder.tsv
3c09557ac425 planemo upload for repository https://github.com/phac-nml/staramr commit 52535d98ad1e03c83c93f230abf966613b4b8fa2
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diff changeset
330 `````````````````
3c09557ac425 planemo upload for repository https://github.com/phac-nml/staramr commit 52535d98ad1e03c83c93f230abf966613b4b8fa2
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diff changeset
331
7
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
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diff changeset
332 A tabular file of each AMR plasmid type and additional BLAST information from the **PlasmidFinder** database, one plasmid type per line.
6
3c09557ac425 planemo upload for repository https://github.com/phac-nml/staramr commit 52535d98ad1e03c83c93f230abf966613b4b8fa2
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diff changeset
333
3c09557ac425 planemo upload for repository https://github.com/phac-nml/staramr commit 52535d98ad1e03c83c93f230abf966613b4b8fa2
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diff changeset
334 +------------+-----------+-----------+----------+-------------------------+-------------+-------+-------+-----------+
7
f1adc47e017a planemo upload for repository https://github.com/phac-nml/staramr commit dad82f0ddadb5e106a54d5aa6609918325e49d23
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parents: 6
diff changeset
335 | Isolate ID | Plasmid | %Identity | %Overlap | HSP Length/Total Length | Contig | Start | End | Accession |
6
3c09557ac425 planemo upload for repository https://github.com/phac-nml/staramr commit 52535d98ad1e03c83c93f230abf966613b4b8fa2
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parents: 5
diff changeset
336 +============+===========+===========+==========+=========================+=============+=======+=======+===========+
3c09557ac425 planemo upload for repository https://github.com/phac-nml/staramr commit 52535d98ad1e03c83c93f230abf966613b4b8fa2
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parents: 5
diff changeset
337 | SRR1952908 | ColpVC | 98.96 | 100 | 193/193 | contig00038 | 1618 | 1426 | JX133088 |
3c09557ac425 planemo upload for repository https://github.com/phac-nml/staramr commit 52535d98ad1e03c83c93f230abf966613b4b8fa2
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parents: 5
diff changeset
338 +------------+-----------+-----------+----------+-------------------------+-------------+-------+-------+-----------+
3c09557ac425 planemo upload for repository https://github.com/phac-nml/staramr commit 52535d98ad1e03c83c93f230abf966613b4b8fa2
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parents: 5
diff changeset
339 | SRR1952908 | IncFIB(S) | 98.91 | 100 | 643/643 | contig00024 | 10302 | 9660 | FN432031 |
3c09557ac425 planemo upload for repository https://github.com/phac-nml/staramr commit 52535d98ad1e03c83c93f230abf966613b4b8fa2
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parents: 5
diff changeset
340 +------------+-----------+-----------+----------+-------------------------+-------------+-------+-------+-----------+
3c09557ac425 planemo upload for repository https://github.com/phac-nml/staramr commit 52535d98ad1e03c83c93f230abf966613b4b8fa2
nml
parents: 5
diff changeset
341 | SRR1952908 | IncFII(S) | 100 | 100 | 262/262 | contig00024 | 54294 | 54555 | CP000858 |
3c09557ac425 planemo upload for repository https://github.com/phac-nml/staramr commit 52535d98ad1e03c83c93f230abf966613b4b8fa2
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parents: 5
diff changeset
342 +------------+-----------+-----------+----------+-------------------------+-------------+-------+-------+-----------+
3c09557ac425 planemo upload for repository https://github.com/phac-nml/staramr commit 52535d98ad1e03c83c93f230abf966613b4b8fa2
nml
parents: 5
diff changeset
343 | SRR1952908 | IncI1 | 100 | 100 | 142/142 | contig00020 | 3907 | 3766 | AP005147 |
3c09557ac425 planemo upload for repository https://github.com/phac-nml/staramr commit 52535d98ad1e03c83c93f230abf966613b4b8fa2
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parents: 5
diff changeset
344 +------------+-----------+-----------+----------+-------------------------+-------------+-------+-------+-----------+
3c09557ac425 planemo upload for repository https://github.com/phac-nml/staramr commit 52535d98ad1e03c83c93f230abf966613b4b8fa2
nml
parents: 5
diff changeset
345 | SRR1952926 | ColpVC | 98.96 | 100 | 193/193 | contig00037 | 657 | 849 | JX133088 |
3c09557ac425 planemo upload for repository https://github.com/phac-nml/staramr commit 52535d98ad1e03c83c93f230abf966613b4b8fa2
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parents: 5
diff changeset
346 +------------+-----------+-----------+----------+-------------------------+-------------+-------+-------+-----------+
3c09557ac425 planemo upload for repository https://github.com/phac-nml/staramr commit 52535d98ad1e03c83c93f230abf966613b4b8fa2
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parents: 5
diff changeset
347 | SRR1952926 | IncFIB(S) | 98.91 | 100 | 643/643 | contig00021 | 10302 | 9660 | FN432031 |
3c09557ac425 planemo upload for repository https://github.com/phac-nml/staramr commit 52535d98ad1e03c83c93f230abf966613b4b8fa2
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parents: 5
diff changeset
348 +------------+-----------+-----------+----------+-------------------------+-------------+-------+-------+-----------+
3c09557ac425 planemo upload for repository https://github.com/phac-nml/staramr commit 52535d98ad1e03c83c93f230abf966613b4b8fa2
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parents: 5
diff changeset
349 | SRR1952926 | IncFII(S) | 100 | 100 | 262/262 | contig00021 | 54294 | 54555 | CP000858 |
3c09557ac425 planemo upload for repository https://github.com/phac-nml/staramr commit 52535d98ad1e03c83c93f230abf966613b4b8fa2
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parents: 5
diff changeset
350 +------------+-----------+-----------+----------+-------------------------+-------------+-------+-------+-----------+
3c09557ac425 planemo upload for repository https://github.com/phac-nml/staramr commit 52535d98ad1e03c83c93f230abf966613b4b8fa2
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parents: 5
diff changeset
351 | SRR1952926 | IncI1 | 100 | 100 | 142/142 | contig00017 | 3907 | 3766 | AP005147 |
3c09557ac425 planemo upload for repository https://github.com/phac-nml/staramr commit 52535d98ad1e03c83c93f230abf966613b4b8fa2
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352 +------------+-----------+-----------+----------+-------------------------+-------------+-------+-------+-----------+
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353
0
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354 pointfinder.tsv
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355 ```````````````
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356
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357 A tabular file of each AMR point mutation and additional BLAST information from the **PointFinder** database, one gene per line.
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358
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359 +-------------+--------------+------------------------------------+--------+-----------+----------------------+------------+-----------+--------------------------+--------------+---------+--------+
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360 | Isolate ID | Gene | Predicted Phenotype | Type | Position | Mutation | %Identity | %Overlap | HSP Length/Total Length | Contig | Start | End |
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361 +=============+==============+====================================+========+===========+======================+============+===========+==========================+==============+=========+========+
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362 | SRR1952908 | gyrA (S83Y) | ciprofloxacin I/R, nalidixic acid | codon | 83 | TCC -> TAC (S -> Y) | 99.96 | 100.00 | 2637/2637 | contig00008 | 22801 | 20165 |
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363 +-------------+--------------+------------------------------------+--------+-----------+----------------------+------------+-----------+--------------------------+--------------+---------+--------+
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364 | SRR1952926 | gyrA (S83Y) | ciprofloxacin I/R, nalidixic acid | codon | 83 | TCC -> TAC (S -> Y) | 99.96 | 100.00 | 2637/2637 | contig00011 | 157768 | 160404 |
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365 +-------------+--------------+------------------------------------+--------+-----------+----------------------+------------+-----------+--------------------------+--------------+---------+--------+
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366
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367 settings.txt
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368 ````````````
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369
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370 The command-line, database versions, and other settings used to run `staramr`.
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371
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372 ::
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373
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374 command_line = staramr search -o out --pointfinder-organism salmonella SRR1952908.fasta SRR1952926.fasta
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5f43703968cf "planemo upload for repository https://github.com/phac-nml/galaxy_tools/tree/master/tools/staramr commit 65ffe1cd11ac6108adf483d3e83856a5466df79c"
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375 version = 0.7.1
6
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376 start_time = 2019-04-25 16:01:32
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377 end_time = 2019-04-25 16:01:34
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378 total_minutes = 0.04
0
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379 resfinder_db_dir = staramr/databases/data/dist/resfinder
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380 resfinder_db_url = https://bitbucket.org/genomicepidemiology/resfinder_db.git
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381 resfinder_db_commit = e8f1eb2585cd9610c4034a54ce7fc4f93aa95535
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382 resfinder_db_date = Mon, 16 Jul 2018 16:58
0
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383 pointfinder_db_dir = staramr/databases/data/dist/pointfinder
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384 pointfinder_db_url = https://bitbucket.org/genomicepidemiology/pointfinder_db.git
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385 pointfinder_db_commit = 8706a6363bb29e47e0e398c53043b037c24b99a7
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386 pointfinder_db_date = Wed, 04 Jul 2018 14:27
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387 plasmidfinder_db_dir = staramr/databases/data/dist/plasmidfinder
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388 plasmidfinder_db_url = https://bitbucket.org/genomicepidemiology/plasmidfinder_db.git
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389 plasmidfinder_db_commit = 81919954cbedaff39056610ab584ab4c06011ed8
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390 plasmidfinder_db_date = Tue, 20 Nov 2018 08:51
0
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391 pointfinder_gene_drug_version = 050218
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392 resfinder_gene_drug_version = 050218.1
0
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393
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394 results.xlsx
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395 ````````````
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396
6
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397 An Excel spreadsheet containing the previous 5 files as separate worksheets.
0
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398
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399 BLAST Hits
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400 ``````````
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401
8
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402 The dataset collection **hits** stores fasta files of the specific blast hits.
0
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403
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404 Galaxy wrapper written by Aaron Petkau at the National Microbiology Laboratory, Public Health Agency of Canada.
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405
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406 .. _staramr: https://github.com/phac-nml/staramr
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407 .. _ResFinder: https://bitbucket.org/genomicepidemiology/resfinder_db
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408 .. _PlasmidFinder: https://bitbucket.org/genomicepidemiology/plasmidfinder_db
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409 .. _PointFinder: https://bitbucket.org/genomicepidemiology/pointfinder_db
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410 .. _webservice: https://cge.cbs.dtu.dk/services/ResFinder/
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411 ]]></help>
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412 <citations>
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413 <citation type="bibtex">
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414 @misc{githubstaramr,
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415 author = {Petkau, Aaron},
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416 year = {2018},
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417 title = {staramr},
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418 publisher = {GitHub},
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419 journal = {GitHub repository},
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420 url = {https://github.com/phac-nml/staramr},
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421 }</citation>
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422 </citations>
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423 </tool>