Mercurial > repos > peterjc > seq_filter_by_id
annotate tools/seq_filter_by_id/README.rst @ 8:2d4537dbf0bc draft
v0.2.6 Depend on Biopython 1.67 from Tool Shed or (Bio)conda
author | peterjc |
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date | Wed, 10 May 2017 13:18:01 -0400 |
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1 Galaxy tool to filter FASTA, FASTQ or SFF sequences by ID |
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2 ========================================================= |
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4 This tool is copyright 2010-2017 by Peter Cock, The James Hutton Institute |
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5 (formerly SCRI, Scottish Crop Research Institute), UK. All rights reserved. |
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6 See the licence text below. |
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7 |
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8 This tool is a short Python script (using both Biopython library functions) |
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9 which divides a FASTA, FASTQ, or SFF file in two, those sequences with or |
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10 without an ID present in the specified column(s) of a tabular file. Example |
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11 uses include filtering based on search results from a tool like NCBI BLAST |
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12 before assembly. |
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13 |
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14 This tool is available from the Galaxy Tool Shed at: |
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15 |
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16 * http://toolshed.g2.bx.psu.edu/view/peterjc/seq_filter_by_id |
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17 |
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18 See also sister tools: |
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19 |
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20 * http://toolshed.g2.bx.psu.edu/view/peterjc/seq_select_by_id |
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21 * http://toolshed.g2.bx.psu.edu/view/peterjc/seq_rename |
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22 * http://toolshed.g2.bx.psu.edu/view/peterjc/seq_filter_by_mapping |
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23 |
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24 |
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25 Automated Installation |
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26 ====================== |
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27 |
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28 This should be straightforward using the Galaxy Tool Shed, which should be |
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29 able to automatically install the dependency on Biopython, and then install |
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30 this tool and run its unit tests. |
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31 |
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32 |
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33 Manual Installation |
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34 =================== |
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35 |
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36 There are just two files to install to use this tool from within Galaxy: |
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37 |
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38 * ``seq_filter_by_id.py`` (the Python script) |
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39 * ``seq_filter_by_id.xml`` (the Galaxy tool definition) |
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40 |
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41 The suggested location is a dedicated ``tools/seq_filter_by_id`` folder. |
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42 |
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43 You will also need to modify the ``tools_conf.xml`` file to tell Galaxy to offer the |
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44 tool. One suggested location is in the filters section. Simply add the line:: |
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45 |
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46 <tool file="seq_filter_by_id/seq_filter_by_id.xml" /> |
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47 |
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48 If you wish to run the unit tests, also move/copy the ``test-data/`` files |
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49 under Galaxy's ``test-data/`` folder. Then:: |
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50 |
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51 $ ./run_tests.sh -id seq_filter_by_id |
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52 |
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53 You will also need to install Biopython 1.54 or later. That's it. |
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54 |
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55 |
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56 History |
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57 ======= |
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58 |
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59 ======= ====================================================================== |
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60 Version Changes |
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61 ------- ---------------------------------------------------------------------- |
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62 v0.0.1 - Initial version, combining three separate scripts for each file format. |
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63 v0.0.4 - Record script version when run from Galaxy. |
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64 - Faster FASTA code which preserves the original line wrapping. |
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65 - Basic unit test included. |
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66 v0.0.5 - Check for errors using Python script's return code. |
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67 - Cope with malformed FASTA entries without an identifier. |
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68 v0.0.6 - Link to Tool Shed added to help text and this documentation. |
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69 - Automated installation of the Biopython dependency. |
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70 - Use reStructuredText for this README file. |
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71 - Adopt standard MIT License. |
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72 - Updated citation information (Cock et al. 2013). |
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73 - Development moved to GitHub, https://github.com/peterjc/pico_galaxy |
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74 - Renamed folder and adopted README.rst naming. |
4 | 75 v0.0.7 - Correct automated dependency definition. |
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76 v0.0.8 - Simplified XML to apply input format to output data. |
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77 v0.2.0 - Can supply ID list as a text parameter (instead of in a file) |
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78 - Using ``optparse`` for the Python command line API. |
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79 - Advanced option to ignore paired read suffices. |
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80 - Updated dependencies to use Biopython 1.64. |
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81 v0.2.1 - Use Biopython instead of Galaxy for FASTQ handling. |
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82 - Tool definition now embeds citation information. |
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83 - Include input dataset name in output dataset names. |
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84 - If white space is found in the requested tabular field then only |
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85 the first word is used as the identifier (with a warning to stderr). |
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86 v0.2.2 - Use the ``format_source=...`` tag. |
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87 - Reorder XML elements (internal change only). |
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88 - Planemo for Tool Shed upload (``.shed.yml``, internal change only). |
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89 v0.2.3 - Ignore blank lines in ID file (contributed by Gildas Le Corguillé). |
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90 - Defensive quoting of filenames etc in the command definition |
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91 (internal change only). |
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92 v0.2.4 - Corrected error message wording. |
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93 v0.2.5 - Ignore empty names, common in R output (Gildas Le Corguillé). |
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94 v0.2.6 - Depends on Biopython 1.67 via legacy Tool Shed package or bioconda. |
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95 ======= ====================================================================== |
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96 |
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97 |
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98 Developers |
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99 ========== |
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100 |
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101 This script and related tools were initially developed on the following hg branches: |
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102 http://bitbucket.org/peterjc/galaxy-central/src/fasta_filter |
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103 http://bitbucket.org/peterjc/galaxy-central/src/tools |
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104 |
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105 Development has now moved to a dedicated GitHub repository: |
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106 https://github.com/peterjc/pico_galaxy/tree/master/tools |
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107 |
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108 For pushing a release to the test or main "Galaxy Tool Shed", use the following |
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109 Planemo commands (which requires you have set your Tool Shed access details in |
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110 ``~/.planemo.yml`` and that you have access rights on the Tool Shed):: |
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111 |
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112 $ planemo shed_update -t testtoolshed --check_diff tools/seq_filter_by_id/ -m "New release" |
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113 ... |
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114 |
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115 or:: |
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116 |
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117 $ planemo shed_update -t toolshed --check_diff tools/seq_filter_by_id/ -m "New release" |
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118 ... |
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119 |
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120 To just build and check the tar ball, use:: |
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121 |
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122 $ planemo shed_upload --tar_only tools/seq_filter_by_id/ |
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123 ... |
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124 $ tar -tzf shed_upload.tar.gz |
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125 test-data/empty_file.dat |
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126 test-data/k12_hypothetical.fasta |
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127 test-data/k12_hypothetical.tabular |
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128 test-data/k12_hypothetical_alt.tabular |
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129 test-data/k12_ten_proteins.fasta |
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130 test-data/sanger-pairs-mixed.fastq |
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131 test-data/sanger-pairs-names.tabular |
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132 test-data/sanger-sample.fastq |
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133 tools/seq_filter_by_id/README.rst |
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134 tools/seq_filter_by_id/seq_filter_by_id.py |
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135 tools/seq_filter_by_id/seq_filter_by_id.xml |
4 | 136 tools/seq_filter_by_id/tool_dependencies.xml |
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137 |
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138 |
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139 Licence (MIT) |
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140 ============= |
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141 |
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142 Permission is hereby granted, free of charge, to any person obtaining a copy |
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143 of this software and associated documentation files (the "Software"), to deal |
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144 in the Software without restriction, including without limitation the rights |
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145 to use, copy, modify, merge, publish, distribute, sublicense, and/or sell |
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146 copies of the Software, and to permit persons to whom the Software is |
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147 furnished to do so, subject to the following conditions: |
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148 |
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149 The above copyright notice and this permission notice shall be included in |
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150 all copies or substantial portions of the Software. |
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151 |
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152 THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR |
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153 IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, |
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154 FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE |
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155 AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER |
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156 LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, |
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157 OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN |
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158 THE SOFTWARE. |