annotate cluster_profiler.xml @ 3:ad1244024bd8 draft

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date Wed, 14 Mar 2018 12:40:35 -0400
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1 <tool id="cluter_profiler" name="clusterProfiler" version="0.1.0">
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2 <description>
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3 GO terms classification and enrichment analysis
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4 </description>
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5 <requirements>
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6 <requirement type="package" version="3.4.1">R</requirement>
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7 <requirement type="package" version="3.5.0">bioconductor-org.hs.eg.db</requirement>
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8 <requirement type="package" version="3.5.0">bioconductor-org.mm.eg.db</requirement>
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9 <requirement type="package" version="3.2.0">bioconductor-dose</requirement>
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10 <requirement type="package" version="3.4.4">bioconductor-clusterprofiler</requirement>
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11 </requirements>
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12 <command detect_errors="exit_code"><![CDATA[
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13 Rscript "$__tool_directory__/GO-enrich.R"
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14 #if $input.ids == "text"
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15 --input_type="text"
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16 --input="$input.text"
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17 #else
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18 --input_type="file"
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19 --input="$input.file"
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20 --ncol="$input.ncol"
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21 --header="$input.header"
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22 #end if
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23
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24 --id_type="$idti.idtypein"
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25
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26 --species="$species"
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27
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28 #if $ggo.go_represent == "true"
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29 --go_represent="true"
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30 --level="$ggo.level"
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31 #else
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32 --go_represent="false"
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33 #end if
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34
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35 #if $ego.go_enrich == "true"
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36 --go_enrich="true"
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37 --pval_cutoff="$ego.pval"
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38 --qval_cutoff="$ego.qval"
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39 #else
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40 --go_enrich="false"
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41 #end if
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42
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43 --onto_opt="$ontology"
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44
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45 --text_output="$text_output"
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46 ]]></command>
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47 <inputs>
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48 <conditional name="input" >
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49 <param name="ids" type="select" label="Provide your identifiers" help="Copy/paste or ID list from a file (e.g. table)" >
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50 <option value="text">Copy/paste your identifiers</option>
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51 <option value="file">Input file containing your identifiers</option>
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52 </param>
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53 <when value="text" >
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54 <param name="txt" type="text" label="Copy/paste your identifiers" help='IDs must be separated by spaces into the form field, for example: P31946 P62258' >
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55 <sanitizer>
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56 <valid initial="string.printable">
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57 <remove value="&apos;"/>
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58 </valid>
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59 <mapping initial="none">
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60 <add source="&apos;" target="__sq__"/>
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61 </mapping>
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62 </sanitizer>
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63 </param>
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64 </when>
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65 <when value="file" >
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66 <param name="file" type="data" format="txt,tabular" label="Choose a file that contains your list of IDs" help="" />
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67 <param name="header" type="boolean" checked="true" truevalue="true" falsevalue="false" label="Does your input file contain header?" />
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68 <param name="ncol" type="text" value="c1" label="The column number of IDs to map" help='For example, fill in "c1" if it is the first column, "c2" if it is the second column and so on' />
0
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69 </when>
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70 </conditional>
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71 <conditional name="idti" >
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72 <param name="idtypein" type="select" label="Select type/source of identifier of your list" help="Please see example of IDs in help section" >
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73 <option value="Uniprot">UniProt accession number</option>
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74 <option value="Entrez">Entrez Gene ID</option>
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75 </param>
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76 <when value="Uniprot"/>
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77 <when value="Entrez"/>
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78 </conditional>
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79 <param name="species" type="select" label="Select a species" >
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80 <option value="human">Human</option>
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81 <option value="mouse">Mouse</option>
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82 <option value="rat">Rat</option>
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83 </param>
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84 <conditional name="ggo">
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85 <param name="go_represent" type="boolean" checked="true" truevalue="true" falsevalue="false" label="Do you want to perform GO categories representation analysis?"/>
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86 <when value="true">
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87 <param name="level" type="select" label="Level of the ontology at which the profile has to be built (the higher this number, the deeper the GO level)">
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88 <option value="1">1</option>
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89 <option value="2">2</option>
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90 <option value="3" selected="True">3</option>
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91 </param>
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92 </when>
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93 <when value="false"/>
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94 </conditional>
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95 <conditional name="ego">
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96 <param name="go_enrich" type="boolean" checked="true" truevalue="true" falsevalue="false" label="Do you want to perform GO categories enrichment analysis?"/>
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97 <when value="true">
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98 <param name="pval" type="float" value="0.01" label="P-value cut off"/>
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99 <param name="qval" type="float" value="0.05" label="Q-value cut off"/>
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100 </when>
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101 <when value="false"/>
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102 </conditional>
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103 <!--conditional name="fun" >
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104 <param name="go_function" type="select" display="checkboxes" multiple="true" label="Please select analyses to perform">
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105 <option value="ggo">GO categories representation</option>
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106 <option value="ego">GO categories enrichment (compared to a background/reference)</option>
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107 </param>
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108 <when value="ggo" >
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109 <param name="level" type="select" label="Level of the ontology at which the profile has to be built (the higher this number, the deeper the GO level)">
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110 <option value="1">1</option>
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111 <option value="2">2</option>
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112 <option value="3" selected="True">3</option>
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113 </param>
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114 </when>
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115 <when value="ego" >
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116 <param name="pval" type="float" value="0.01" label="P-value cut off"/>
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117 <param name="qval" type="float" value="0.05" label="Q-value cut off"/>
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118 </when>
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119 </conditional-->
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120
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121 <param name="ontology" type="select" display="checkboxes" multiple="true" label="Please select GO terms category">
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122 <option value="CC">Cellular Component</option>
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123 <option value="BP">Biological Process</option>
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124 <option value="MF">Molecular Function</option>
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125 </param>
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126
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127
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128
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129 </inputs>
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130 <outputs>
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131 <data name="text_output" format="tabular" label="clusterProfiler text output" />
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132 <collection type="list" label="clusterProfiler diagram outputs" name="output" >
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133 <discover_datasets pattern="(?P&lt;designation&gt;.+\.png)" ext="png" />
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134 </collection>
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135 </outputs>
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136 <tests>
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137 <test>
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138 <conditional name="input">
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139 <param name="ids" value="file"/>
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140 <param name="file" value="Lacombe_et_al_2017_OK.txt"/>
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141 <param name="header" value="true"/>
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142 <param name="ncol" value="c1"/>
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143 </conditional>
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144 <conditional name="idti">
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145 <param name="idtypein" value="Uniprot"/>
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146 </conditional>
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147 <param name="species" value="human"/>
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148 <conditional name="ggo">
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149 <param name="go_represent" value="true"/>
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150 <param name="level" value="3"/>
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151 </conditional>
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152 <conditional name="ego">
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153 <param name="go_enrich" value="false"/>
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154 </conditional>
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155 <param name="ontology" value="CC"/>
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156 <output name="text_output" file="clusterProfiler_text_output.tabular"/>
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157 <output_collection name="output">
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158 <element name="clusterProfiler_diagram_outputs__GGO.CC.png" file="clusterProfiler_diagram_outputs__GGO.CC.png" ftype="png"/>
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159 </output_collection>
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160 </test>
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161 </tests>
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162 <help><![CDATA[
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163
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164 **Galaxy component based on R package clusterProfiler (see ref below)**
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165
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166 This component allows to perform GO enrichment-analyses.
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167 Given a list of IDs, the tool either
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168 (i) performs gene classification based on GO distribution at a specific level, or
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169 (ii) calculates GO categories enrichment (over- or under-representation) for the IDs of the input list,
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170 compared to a background (whole organism or user-defined list).
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171
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172 **Input required**
1
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173
0
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174 This component works with Gene ids (e.g : 4151, 7412) or Uniprot accession number (e.g. P31946).
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175 You can copy/paste these identifiers or supply a tabular file (.csv, .tsv, .txt, .tab) where there are contained.
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176
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177
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178 **Output**
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179
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180 Text (tables) and graphics representing the repartition and/or enrichment of GO categories.
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181
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182 **User manual / Documentation** of the clusterProfiler R package (functions and parameters):
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183 https://bioconductor.org/packages/3.7/bioc/vignettes/clusterProfiler/inst/doc/clusterProfiler.html
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184 (Very well explained)
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185
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186 **Reference**
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187
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188 clusterProfiler R package reference :
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189 G Yu, LG Wang, Y Han, QY He. clusterProfiler: an R package for comparing biological themes among gene clusters.
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190 OMICS: A Journal of Integrative Biology 2012, 16(5):284-287.
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191 doi:[10.1089/omi.2011.0118](http://dx.doi.org/10.1089/omi.2011.0118)
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192
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193
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194 **Galaxy integration**
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195
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196 T.P. Lien Nguyen, Florence Combes, Yves Vandenbrouck CEA, INSERM, CNRS, Grenoble-Alpes University, BIG Institute, FR
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197
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198 Sandra Dérozier, Olivier Rué, Christophe Caron, Valentin Loux INRA, Paris-Saclay University, MAIAGE Unit, Migale Bioinformatics platform
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199
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200 This work has been partially funded through the French National Agency for Research (ANR) IFB project.
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201
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202 Contact support@proteore.org for any questions or concerns about the Galaxy implementation of this tool.
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203
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204
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205 ]]></help>
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206 <citations>
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207 </citations>
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208 </tool>