LOCUS       .                       2523 bp    DNA              UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     terminator      392..419
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(431..1018)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      1040..1069
                     /note="T3Te terminator"
                     /note="phage T3 early transcription terminator"
     misc_feature    1143..1148
                     /note="BsmBI"
     source          5..1149
                     /source="Exported"
     CDS             1154..1861
                     /codon_start=1
                     /note="mRuby2"
                     /product="monomeric red fluorescent protein derived from
                     mRuby, with improved photophysical properties (Lam et al.,
                     2012)"
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHM
                     ALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVA
                     KFAGLGGGMDELYK"
     source          1154..1863
                     /source="Exported"
     misc_feature    complement(1869..1874)
                     /note="BsmBI"
     terminator      1941..1972
                     /note="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     promoter        1973..2075
                     /note="cat promoter"
                     /note="promoter of the E. coli cat gene"
     source          1868..2523
                     /source="Exported"
ORIGIN
        1 ctcaggcgca atcacgaatg aataacggtt tggttggtgc gagtgatttt gatgacgagc
       61 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg ccattctcac
      121 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
      181 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
      241 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
      301 aatatggtat tgataatcct gatatgaata aattgcagtt tcacttgatg ctcgatgagt
      361 ttttctaatg agggcccaaa tgtaatcacc tggctcacct tcgggtgggc ctttctgcgt
      421 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgatgctcaa
      481 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
      541 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
      601 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
      661 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
      721 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
      781 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
      841 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
      901 agccagttac ctcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
      961 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1021 agatcctttg attttctacc gaagaaaggc ccacccgtga aggtgagcca gtgagttgat
     1081 tgcagtccag ttacgctgga gtctgaggct cgtcctgaat gatatcaagc ttgaattcgt
     1141 tacgtctcgc agcgtgtcta agggcgaaga gctgatcaag gaaaatatgc gtatgaaggt
     1201 ggtcatggaa ggttcggtca acggccacca attcaaatgc acaggtgaag gagaaggcaa
     1261 tccgtacatg ggaactcaaa ccatgaggat caaagtcatc gagggaggac ccctgccatt
     1321 tgcctttgac attcttgcca cgtcgttcat gtatggcagc cgtactttta tcaagtaccc
     1381 gaaaggcatt cctgatttct ttaaacagtc ctttcctgag ggttttactt gggaaagagt
     1441 tacgagatac gaagatggtg gagtcgtcac cgtcatgcag gacaccagcc ttgaggatgg
     1501 ctgtctcgtt taccacgtcc aagtcagagg ggtaaacttt ccctccaatg gtcccgtgat
     1561 gcagaagaag accaagggtt gggagcctaa tacagagatg atgtatccag cagatggtgg
     1621 tctgagggga tacactcata tggcactgaa agttgatggt ggtggccatc tgtcttgctc
     1681 tttcgtaaca acttacaggt caaaaaagac cgtcgggaac atcaagatgc ccggtatcca
     1741 tgccgttgat caccgcctgg aaaggttaga ggaaagtgac aatgaaatgt tcgtagtaca
     1801 acgcgaacac gcagttgcca agttcgccgg gcttggtggt gggatggacg agctgtacaa
     1861 gtcaggccga gacgaagacg aattctctag atatcgctca atactgacca tttaaatcat
     1921 acctgacctc catagcagaa agtcaaaagc ctccgaccgg aggcttttga cttgatcggc
     1981 acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg
     2041 agttatcgag attttcagga gctaaggaag ctaaaatgag ccatattcaa cgggaaacgt
     2101 cttgctcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
     2161 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg
     2221 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
     2281 tggtcaggct aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
     2341 gtactcctga tgatgcatgg ttactcacca ctgcgatccc agggaaaaca gcattccagg
     2401 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
     2461 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cggcgatcgc gtatttcgtc
     2521 tcg
//