annotate test-data/HC_Amp_ccdB.gb @ 2:dad2c4c3450a draft default tip

planemo upload for repository https://github.com/brsynth/galaxytools/tree/main/tools commit db4ac861e1d03fcdfe94321d858839124e493930-dirty
author tduigou
date Wed, 23 Jul 2025 09:44:33 +0000
parents 9f845ccb1a9f
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1 LOCUS Exported 2721 bp ds-DNA circular SYN 14-SEP-2017
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2 DEFINITION synthetic circular DNA.
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3 ACCESSION .
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4 VERSION .
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5 KEYWORDS HC_Amp_ccdB(1-25).gb
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6 SOURCE synthetic DNA construct
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7 ORGANISM synthetic DNA construct
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8 REFERENCE 1 (bases 1 to 2721)
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9 AUTHORS Trial User
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10 TITLE Direct Submission
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11 JOURNAL Exported Sep 14, 2017 from SnapGene Viewer 4.0.2
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12 http://www.snapgene.com
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13 FEATURES Location/Qualifiers
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14 source 1..2721
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15 /organism="synthetic DNA construct"
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16 /mol_type="other DNA"
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17 misc_feature complement(73..78)
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18 /label=BsmBI
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19 misc_feature 129..462
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20 /label=*ccdB promoter*
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21 exon 463..768
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22 /label=ccdB
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23 /note="ccdB"
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24 terminator 809..880
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25 /note="rrnB T1 terminator
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26 transcription terminator T1 from the E. coli rrnB gene"
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27 terminator 896..923
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28 /note="T7Te terminator
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29 phage T7 early transcription terminator"
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30 misc_feature 930..942
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31 /label=BioBrick suffix
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32 /note="universal suffix for all parts"
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33 misc_feature 943..948
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34 /label=BsmBI
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35 terminator 1027..1056
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36 /note="T3Te terminator
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37 phage T3 early transcription terminator"
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38 rep_origin 1078..1665
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39 /direction=RIGHT
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40 /label=ori
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41 /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
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42 replication"
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43 terminator 1677..1704
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44 /note="T7Te terminator
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45 phage T7 early transcription terminator"
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46 CDS complement(1728..2588)
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47 /codon_start=1
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48 /gene="bla"
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49 /product="beta-lactamase"
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50 /note="AmpR
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51 confers resistance to ampicillin, carbenicillin, and
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52 related antibiotics"
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53 /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
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54 ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
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55 PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
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56 EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
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57 LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
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58 LIKHW"
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59 promoter complement(2589..2691)
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60 /note="cat promoter
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61 promoter of the E. coli cat gene"
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62 ORIGIN
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63 1 ctttctgcta tggaggtcag gtatgattta aatggtcagt attgagcgat atctagagaa
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64 61 ttcgtcatag gagagacgca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
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65 121 aatgcaggga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt
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66 181 ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct
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67 241 atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat
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68 301 atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc
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69 361 gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg
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70 421 aaatgaacgg ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac
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71 481 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac
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72 541 acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc
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73 601 ccccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc
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74 661 gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc
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75 721 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataagc tgatagtgct
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76 781 agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
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77 841 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
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78 901 accttcgggt gggcctttct gcgtttatat actagtagcg gccgtctcaa cgataacgaa
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79 961 ttcaagcttg atatcattca ggacgagcct cagactccag cgtaactgga ctgcaatcaa
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80 1021 ctcactggct caccttcacg ggtgggcctt tcttcggtag aaaatcaaag gatcttcttg
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81 1081 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
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82 1141 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaggtaac tggcttcagc
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83 1201 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
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84 1261 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
9f845ccb1a9f planemo upload for repository https://github.com/brsynth/galaxytools/tree/main/tools commit 3401816c949b538bd9c67e61cbe92badff6a4007-dirty
tduigou
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85 1321 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
9f845ccb1a9f planemo upload for repository https://github.com/brsynth/galaxytools/tree/main/tools commit 3401816c949b538bd9c67e61cbe92badff6a4007-dirty
tduigou
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86 1381 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
9f845ccb1a9f planemo upload for repository https://github.com/brsynth/galaxytools/tree/main/tools commit 3401816c949b538bd9c67e61cbe92badff6a4007-dirty
tduigou
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87 1441 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
9f845ccb1a9f planemo upload for repository https://github.com/brsynth/galaxytools/tree/main/tools commit 3401816c949b538bd9c67e61cbe92badff6a4007-dirty
tduigou
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diff changeset
88 1501 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
9f845ccb1a9f planemo upload for repository https://github.com/brsynth/galaxytools/tree/main/tools commit 3401816c949b538bd9c67e61cbe92badff6a4007-dirty
tduigou
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diff changeset
89 1561 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
9f845ccb1a9f planemo upload for repository https://github.com/brsynth/galaxytools/tree/main/tools commit 3401816c949b538bd9c67e61cbe92badff6a4007-dirty
tduigou
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diff changeset
90 1621 catcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgca
9f845ccb1a9f planemo upload for repository https://github.com/brsynth/galaxytools/tree/main/tools commit 3401816c949b538bd9c67e61cbe92badff6a4007-dirty
tduigou
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91 1681 gaaaggccca cccgaaggtg agccaggtga ttacatttgg gccctcatta ccaatgctta
9f845ccb1a9f planemo upload for repository https://github.com/brsynth/galaxytools/tree/main/tools commit 3401816c949b538bd9c67e61cbe92badff6a4007-dirty
tduigou
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92 1741 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
9f845ccb1a9f planemo upload for repository https://github.com/brsynth/galaxytools/tree/main/tools commit 3401816c949b538bd9c67e61cbe92badff6a4007-dirty
tduigou
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93 1801 cccgtcgtgt agataactac gatgcgggag ggcttaccat ctggccccag tgctgcaatg
9f845ccb1a9f planemo upload for repository https://github.com/brsynth/galaxytools/tree/main/tools commit 3401816c949b538bd9c67e61cbe92badff6a4007-dirty
tduigou
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94 1861 ataccgcgag aaccacgctc accggctcca gatttatcag caataaacca gccagccggg
9f845ccb1a9f planemo upload for repository https://github.com/brsynth/galaxytools/tree/main/tools commit 3401816c949b538bd9c67e61cbe92badff6a4007-dirty
tduigou
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95 1921 agggccgagc gcagaagtga tcctgcaact ttatccgcct ccatccagtc tattaattgt
9f845ccb1a9f planemo upload for repository https://github.com/brsynth/galaxytools/tree/main/tools commit 3401816c949b538bd9c67e61cbe92badff6a4007-dirty
tduigou
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96 1981 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
9f845ccb1a9f planemo upload for repository https://github.com/brsynth/galaxytools/tree/main/tools commit 3401816c949b538bd9c67e61cbe92badff6a4007-dirty
tduigou
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97 2041 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
9f845ccb1a9f planemo upload for repository https://github.com/brsynth/galaxytools/tree/main/tools commit 3401816c949b538bd9c67e61cbe92badff6a4007-dirty
tduigou
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98 2101 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
9f845ccb1a9f planemo upload for repository https://github.com/brsynth/galaxytools/tree/main/tools commit 3401816c949b538bd9c67e61cbe92badff6a4007-dirty
tduigou
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diff changeset
99 2161 ggtcctccga tcgttgccag aagtaagttg gccgcagtgt tatcactcat ggttatggca
9f845ccb1a9f planemo upload for repository https://github.com/brsynth/galaxytools/tree/main/tools commit 3401816c949b538bd9c67e61cbe92badff6a4007-dirty
tduigou
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100 2221 gcactgcata attctcttac tgtcatgcca tccgtgagat gcttttctgt gactggtgag
9f845ccb1a9f planemo upload for repository https://github.com/brsynth/galaxytools/tree/main/tools commit 3401816c949b538bd9c67e61cbe92badff6a4007-dirty
tduigou
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101 2281 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
9f845ccb1a9f planemo upload for repository https://github.com/brsynth/galaxytools/tree/main/tools commit 3401816c949b538bd9c67e61cbe92badff6a4007-dirty
tduigou
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102 2341 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
9f845ccb1a9f planemo upload for repository https://github.com/brsynth/galaxytools/tree/main/tools commit 3401816c949b538bd9c67e61cbe92badff6a4007-dirty
tduigou
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103 2401 cgttcttcgg ggcgtaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
9f845ccb1a9f planemo upload for repository https://github.com/brsynth/galaxytools/tree/main/tools commit 3401816c949b538bd9c67e61cbe92badff6a4007-dirty
tduigou
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104 2461 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
9f845ccb1a9f planemo upload for repository https://github.com/brsynth/galaxytools/tree/main/tools commit 3401816c949b538bd9c67e61cbe92badff6a4007-dirty
tduigou
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105 2521 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
9f845ccb1a9f planemo upload for repository https://github.com/brsynth/galaxytools/tree/main/tools commit 3401816c949b538bd9c67e61cbe92badff6a4007-dirty
tduigou
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106 2581 atactcattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
9f845ccb1a9f planemo upload for repository https://github.com/brsynth/galaxytools/tree/main/tools commit 3401816c949b538bd9c67e61cbe92badff6a4007-dirty
tduigou
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107 2641 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aagtcaaaag
9f845ccb1a9f planemo upload for repository https://github.com/brsynth/galaxytools/tree/main/tools commit 3401816c949b538bd9c67e61cbe92badff6a4007-dirty
tduigou
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diff changeset
108 2701 cctccggtcg gaggcttttg a
9f845ccb1a9f planemo upload for repository https://github.com/brsynth/galaxytools/tree/main/tools commit 3401816c949b538bd9c67e61cbe92badff6a4007-dirty
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109 //