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comparison fastx_toolkit-0.0.6/galaxy/tools/fastx_toolkit/fasta_clipping_histogram.xml @ 3:997f5136985f draft default tip
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| author | xilinxu |
|---|---|
| date | Thu, 14 Aug 2014 04:52:17 -0400 |
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| 2:dfe9332138cf | 3:997f5136985f |
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| 1 <tool id="cshl_fasta_clipping_histogram" name="Length Distribution"> | |
| 2 <description>chart</description> | |
| 3 <command>fasta_clipping_histogram.pl $input $outfile</command> | |
| 4 | |
| 5 <inputs> | |
| 6 <param format="fasta" name="input" type="data" label="Library to analyze" /> | |
| 7 </inputs> | |
| 8 | |
| 9 <outputs> | |
| 10 <data format="png" name="outfile" metadata_source="input" /> | |
| 11 </outputs> | |
| 12 <help> | |
| 13 | |
| 14 **What it does** | |
| 15 | |
| 16 This tool creates a histogram image of sequence lengths distribution in a given fasta data set file. | |
| 17 | |
| 18 **TIP:** Use this tool after clipping your library (with **FASTX Clipper tool**), to visualize the clipping results. | |
| 19 | |
| 20 ----- | |
| 21 | |
| 22 **Output Examples** | |
| 23 | |
| 24 | |
| 25 In the following library, most sequences are 24-mers to 27-mers. | |
| 26 This could indicate an abundance of endo-siRNAs (depending of course of what you've tried to sequence in the first place). | |
| 27 | |
| 28 .. image:: ./static/fastx_icons/fasta_clipping_histogram_1.png | |
| 29 | |
| 30 | |
| 31 In the following library, most sequences are 19,22 or 23-mers. | |
| 32 This could indicate an abundance of miRNAs (depending of course of what you've tried to sequence in the first place). | |
| 33 | |
| 34 .. image:: ./static/fastx_icons/fasta_clipping_histogram_2.png | |
| 35 | |
| 36 </help> | |
| 37 </tool> | |
| 38 <!-- FASTA-Clipping-Histogram is part of the FASTX-toolkit, by A.Gordon (gordon@cshl.edu) --> |