view fastx_toolkit-0.0.6/galaxy/tools/fastx_toolkit/fasta_clipping_histogram.xml @ 3:997f5136985f draft default tip

Uploaded
author xilinxu
date Thu, 14 Aug 2014 04:52:17 -0400
parents
children
line wrap: on
line source

<tool id="cshl_fasta_clipping_histogram" name="Length Distribution">
	<description>chart</description>
	<command>fasta_clipping_histogram.pl $input $outfile</command>
	
	<inputs>
		<param format="fasta" name="input" type="data" label="Library to analyze" />
	</inputs>

	<outputs>
		<data format="png" name="outfile" metadata_source="input" />
	</outputs>
<help>

**What it does**

This tool creates a histogram image of sequence lengths distribution in a given fasta data set file.

**TIP:** Use this tool after clipping your library (with **FASTX Clipper tool**), to visualize the clipping results.

-----

**Output Examples**


In the following library, most sequences are 24-mers to 27-mers. 
This could indicate an abundance of endo-siRNAs (depending of course of what you've tried to sequence in the first place).

.. image:: ./static/fastx_icons/fasta_clipping_histogram_1.png


In the following library, most sequences are 19,22 or 23-mers. 
This could indicate an abundance of miRNAs (depending of course of what you've tried to sequence in the first place).

.. image:: ./static/fastx_icons/fasta_clipping_histogram_2.png

</help>
</tool>
<!-- FASTA-Clipping-Histogram is part of the FASTX-toolkit, by A.Gordon (gordon@cshl.edu) -->