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3
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1 <tool id="cshl_fasta_clipping_histogram" name="Length Distribution">
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2 <description>chart</description>
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3 <command>fasta_clipping_histogram.pl $input $outfile</command>
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4
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5 <inputs>
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6 <param format="fasta" name="input" type="data" label="Library to analyze" />
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7 </inputs>
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8
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9 <outputs>
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10 <data format="png" name="outfile" metadata_source="input" />
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11 </outputs>
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12 <help>
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13
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14 **What it does**
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15
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16 This tool creates a histogram image of sequence lengths distribution in a given fasta data set file.
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17
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18 **TIP:** Use this tool after clipping your library (with **FASTX Clipper tool**), to visualize the clipping results.
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19
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20 -----
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21
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22 **Output Examples**
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23
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24
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25 In the following library, most sequences are 24-mers to 27-mers.
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26 This could indicate an abundance of endo-siRNAs (depending of course of what you've tried to sequence in the first place).
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27
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28 .. image:: ./static/fastx_icons/fasta_clipping_histogram_1.png
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29
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30
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31 In the following library, most sequences are 19,22 or 23-mers.
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32 This could indicate an abundance of miRNAs (depending of course of what you've tried to sequence in the first place).
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33
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34 .. image:: ./static/fastx_icons/fasta_clipping_histogram_2.png
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35
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36 </help>
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37 </tool>
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38 <!-- FASTA-Clipping-Histogram is part of the FASTX-toolkit, by A.Gordon (gordon@cshl.edu) -->
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