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view fastx_toolkit-0.0.6/galaxy/tools/fastx_toolkit/fasta_clipping_histogram.xml @ 3:997f5136985f draft default tip
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author | xilinxu |
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date | Thu, 14 Aug 2014 04:52:17 -0400 |
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<tool id="cshl_fasta_clipping_histogram" name="Length Distribution"> <description>chart</description> <command>fasta_clipping_histogram.pl $input $outfile</command> <inputs> <param format="fasta" name="input" type="data" label="Library to analyze" /> </inputs> <outputs> <data format="png" name="outfile" metadata_source="input" /> </outputs> <help> **What it does** This tool creates a histogram image of sequence lengths distribution in a given fasta data set file. **TIP:** Use this tool after clipping your library (with **FASTX Clipper tool**), to visualize the clipping results. ----- **Output Examples** In the following library, most sequences are 24-mers to 27-mers. This could indicate an abundance of endo-siRNAs (depending of course of what you've tried to sequence in the first place). .. image:: ./static/fastx_icons/fasta_clipping_histogram_1.png In the following library, most sequences are 19,22 or 23-mers. This could indicate an abundance of miRNAs (depending of course of what you've tried to sequence in the first place). .. image:: ./static/fastx_icons/fasta_clipping_histogram_2.png </help> </tool> <!-- FASTA-Clipping-Histogram is part of the FASTX-toolkit, by A.Gordon (gordon@cshl.edu) -->