Mercurial > repos > mheinzl > fsd_bvsa
annotate fsd_beforevsafter.xml @ 6:92c80d62d8e2 draft
planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit c5adfd9bcda9cd7a6ba6b7fe824dcf765f7afbee
author | mheinzl |
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date | Wed, 23 May 2018 15:02:12 -0400 |
parents | 1eae0524b285 |
children | c357ce2783a4 |
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planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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1 <?xml version="1.0" encoding="UTF-8"?> |
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planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit c5adfd9bcda9cd7a6ba6b7fe824dcf765f7afbee
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2 <tool id="fsd_beforevsafter" name="Duplex Sequencing Analysis: fsd_beforevsafter" version="0.0.8"> |
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planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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3 <requirements> |
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planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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4 <!-- galaxy version 16.04 --> |
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planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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5 <requirement type="package" version="2.7">python</requirement> |
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planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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6 <requirement type="package" version="1.4">matplotlib</requirement> |
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planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 434a4c969d587d4b14cc1642e38b806af2151953
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7 <requirement type="package" version="1.71">biopython</requirement> |
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planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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8 |
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planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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9 </requirements> |
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planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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10 <description>Family size distribution (FSD) of tags from various steps of the Du Novo pipeline</description> |
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planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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11 <command> |
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12 python2 $__tool_directory__/fsd_beforevsafter.py --inputFile_SSCS "$file1" --inputName1 "$file1.name" --makeDCS "$makeDCS" --afterTrimming "$afterTrimming" --alignedTags "$alignedTags" --sep $separator --output_pdf $output_pdf --output_csv $output_csv |
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planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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13 </command> |
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planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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14 <inputs> |
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planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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15 <param name="file1" type="data" format="tabular" label="Dataset 1: input tags of whole dataset" optional="false" help="Input in tabular format with the family size, tags and the direction of the strand ('ab' or 'ba') for each family."/> |
6716b1cddf3e
planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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16 <param name="makeDCS" type="data" format="fasta" label="Dataset 2: tags after making DCSs" help="Input in fasta format with the tags of the reads, which were aligned to DCSs, and their family sizes of both strands (reverse and forward) in the header, as well as the read itself in the next line."/> |
6716b1cddf3e
planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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17 <param name="afterTrimming" type="data" format="fasta" optional="true" label="Dataset 3: tags after trimming" help="Input in fasta format with the tags of the reads, which were not filtered out after trimming, and their family sizes of both strands (reverse and forward) in the header, as well as the read itself in the next following line."/> |
6716b1cddf3e
planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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18 <param name="alignedTags" type="data" format="txt" optional="true" label="Dataset 4: input tags aligned to the reference genome" help="Input in txt format with the regions and the tags, which were aligned to the reference genome."/> |
6716b1cddf3e
planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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19 <param name="separator" type="text" label="Separator of the CSV file." help="can be a single character" value=","/> |
6716b1cddf3e
planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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20 </inputs> |
6716b1cddf3e
planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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21 <outputs> |
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planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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22 <data name="output_pdf" format="pdf" /> |
6716b1cddf3e
planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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23 <data name="output_csv" format="csv"/> |
6716b1cddf3e
planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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24 </outputs> |
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planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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25 <help> <![CDATA[ |
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planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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26 |
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planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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27 **What it does** |
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planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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28 |
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planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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29 This tool will create a distribution of family sizes of various datasets obtained from different steps of the Du Novo pipeline. |
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planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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30 |
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planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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31 **Input** |
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32 |
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planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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33 **Dataset 1:** This tools expects a tabular file with the tags of all families, their sizes and information about forward (ab) and reverse (ba) strands. |
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planemo upload for repository https://github.com/monikaheinzl/galaxyProject/tree/master/tools/fsd_beforevsafter commit 6055f8c5c052f528ff85fb5e0d43b4500830637a
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34 |
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35 +-----+----------------------------+----+ |
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36 | 1 | AAAAAAAAAAAATGTTGGAATCTT | ba | |
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37 +-----+----------------------------+----+ |
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38 | 10 | AAAAAAAAAAAGGCGGTCCACCCC | ab | |
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39 +-----+----------------------------+----+ |
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40 | 28 | AAAAAAAAAAATGGTATGGACCGA | ab | |
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41 +-----+----------------------------+----+ |
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42 |
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43 |
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44 |
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45 **Dataset 2:** And a fasta file with all tags and their family sizes of both strands (forward and reverse) in the header and the read itself in the next line is required. This input file can be obtained by Du Novo: make consensus reads. |
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46 |
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47 |
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48 **Dataset 3 (optional):** In addition, the fasta file with all tags, which were not filtered out after trimming, can be given. |
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49 For both input files, only one file from both tools are necessary (these tools give for both forward and reverse strands an output file), since both files have the same tags and family sizes, but different reads, which are not required in this tool. |
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50 |
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51 +-------------------------------------------+ |
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52 | >AAAAAAAAATAGATCATAGACTCT 7-10 | |
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53 | | |
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54 | CTAGACTCACTGGCGTTACTGACTGCGAGACCCTCCACGTG | |
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55 +-------------------------------------------+ |
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56 | >AAAAAAAAGGCAGAAGATATACGC 11-3 | |
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57 | | |
0
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58 | CNCNGGCCCCCCGCTCCGTGCACAGACGNNGCNACTGACAA | |
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59 +-------------------------------------------+ |
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60 |
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61 |
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62 |
2
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63 |
5
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64 **Dataset 4 (optional):** Finally, a TXT file with the regions and all tags that were aligned to the reference genome can be given as input. This file can obtained from "Duplex Sequencing Analysis: range2tag" |
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65 |
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66 +-----------+------------------------------+ |
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67 | 87_636 | AAATCAAAGTATGAATGAAGTTGCCT | |
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68 +-----------+------------------------------+ |
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69 | 87_636 | AAATTCATAGCATTAATTTCAACGGG | |
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70 +-----------+------------------------------+ |
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71 | 656_1143 | GGGGCAGCCATATTGGCAATTATCAT | |
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72 +-----------+------------------------------+ |
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73 |
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74 **Output** |
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75 |
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76 The output is a PDF file with the plot and a CSV with the data of the plot. |
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77 |
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78 |
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79 **About Author** |
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80 |
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81 Author: Monika Heinzl |
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82 |
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83 Department: Institute of Bioinformatics, Johannes Kepler University Linz, Austria |
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84 |
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85 Contact: monika.heinzl@edumail.at |
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86 |
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87 ]]> |
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88 |
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89 </help> |
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90 <citations> |
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91 <citation type="bibtex"> |
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92 @misc{duplex, |
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93 author = {Heinzl, Monika}, |
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94 year = {2018}, |
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95 title = {Development of algorithms for the analysis of duplex sequencing data} |
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96 } |
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97 </citation> |
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98 </citations> |
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99 </tool> |